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The Title Goes Here with Each Initial Letter Capitalized

First Author a, *, Second Author b, Third Author c

aFirst affiliation, Address, City and Postcode, Country

bSecond affiliation, Address, City and Postcode, Country

cThird affiliation, Address, City and Postcode, Country

Abstract

Click here and insert your abstract text. The abstract should consist of a single paragraph containing 100-200 words. It should be a summary of the paper and not an introduction. It should present concisely the objectives, methods used, results obtained, and their significance.

Key Words: Type your keywords (3-5) here, separated by semicolons (;)

1. Introduction

This template is intended to be a tool to improve manuscript clarity for the reviewers. The final layout of the typeset paper will not match this template layout [1].

In this section, provide a description of the background and aims of the manuscript [2]. Avoid a detail literature review or summary of the results. You can mention the appropriate location of the figure(s) here (Fig. 1.). However, 1

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please supply your figure(s) separately from this text [3]. Here follows further author guidelines.

Fig. 1. First picture, please type the title of figure 1 here.

2.

Materials and methods

2.1. Chemicals

Provide sufficient detail methods to allow the work to be reproduced.

Methods already published should be indicated by a reference. Only relevant modifications should be described. Please state specific material and equipment used in the research. Sub section titles in this template are given as examples only. Please write your own sub section titles in accordance with your research.

2.2.

Plant materials

………

……….

……….

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2.3. Method development and validation 2.3.1. HPLC instrumentation and condition

………

………

……….

2.4. Data analysis

………

………...

………

………..

………

……….

3.

Results and discussion

3.1.

Isolation and identification of compound AA

Results should be clear and concise. Discuss the significance of the results of the work. Avoid extensive citations and discussion of published literature.

1 2 3 4 5 6

7 8 9 10 11 12 13 14 15 16 17 18 19 20

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3.2.

Method validation

3.2.1. Linearity, limit of detection (LOD) and limit of quantification (LOQ

………

………

……… (Table 1).

3.3.

Quantification of compound AA in crude extract of plant A

………

………

………

……… (Fig. 2).

Fig. 2. Second picture, please type the title of figure 2 here.

4.

Conclusions

Present the conclusion which is the answer of research questions in a short paragraph.

Acknowledgement

This unnumbered section is used to identify people who have aided the authors in accomplishing the work presented, to state conflicts of interest, and 1

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References

[1] Avanti C, Oktaviani NA, Hinrichs WL, Frijlink HW, Mulder FA. Aspartate buffer and divalent metal ions affect oxytocin in aqueous solution and protect it from degradation. International journal of pharmaceutics.

2013;444(1):139-45.

[2] Yunita O, Yuwono M, Rantam FA. In vitro cytotoxicity assay of Sauropus androgynus on human mesenchymal stem cells. Toxicological &

Environmental Chemistry. 2013;95(4):679-86.

[3] Sutarjadi, Rahman A, Indrawati NL. Jamu: Obat asli Indonesia pusaka leluhur warisan nasional bangsa. Jakarta: PT Gramedia Pustaka Utama;

2012.

Table 1

An example of a table

An example of a column heading Column A (t) Column B (T)

And an entry 1 2

And another entry 3 4

And another entry 5 6

Figure captions

Fig. 1. First picture, please type the title of figure 1 here.

Fig. 2. Second picture, please type the title of figure 2 here.

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16 17 18 19 20 21 22

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1 2 3

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