14 PERBEDAAN EKSPRESI PROTEIN 53
TERHADAP RESPON TERAPI RADIASI PADA KANKER SERVIKS
Lydia Irtifany Lubis, Sarah Dina, Roy Yustin Simanjuntak, Hotma Partogi Pasaribu, Henry Salim Siregar, Riza Rivany
Departemen Obstetri dan Ginekologi
Fakultas Kedokteran Universitas Sumatera Utara
Medan, Februari 2017
Abstrak
Tujuan: Untuk mengetahui perbedaan ekspresi protein 53 terhadap respon terapi radiasi pada kanker serviks.
Metode: Penelitian analitik dengan rancangan case control dilakukan di Departemen Obstetri dan Ginekologi FK USU RSUP H. Adam Malik dan Departemen Patologi Anatomi RSUP H. Adam Malik Medan mulai bulan Januari sampai Februari 2017. Sebanyak 15 blok parafin yang terdiri dari 15 blok parafin kanker serviks respon tidak komplit sebagai kasus dan 15 blok parafin kanker serviks respon komplit sebagai kontrol yang belum menjalani terapi radiasi dikumpulkan secara konsekutif dan dilakukan penilaian imunohistokimia ekspresi p53 dinilai terhadap jumlah apoptosis dan respon radiasi. Respon radiasi dinilai berdasarkan pemeriksaan klinis dan kriteria RECIST dan untuk jumlah apoptosis dilakukan dengan pewarnaan Hematoxylin - Eosin dan dihitung jumlah sel apoptosis per 5 LPB dengan menggunakan mikroskop cahaya. Analisa data menggunakan uji Kruskal – Wallis. Interval kepercayaan 83.5% dan p<0.05 bermakna secara statistik.
Hasil: Dari 30 blok parafin diperoleh 15 blok parafin dengan respon komplit dan 15 blok parafin dengan respon tidak komplit. Peningkatan ekspresi p53 dijumpai pada jumlah apoptosis banyak (≥ 5 sel apoptosis/5 LPB) dengan nilai p = 0.033, dan juga dijumpai peningkatan ekspresi p53 pada respon radiasi tidak komplit (nilai p = 0.046).
Kesimpulan: Ekspresi p53 sebelum terapi radiasi dapat dijadikan pertanda awal dalam keberhasilan respon terapi radiasi pada kanker serviks.
15 THE DIFFERENCE OF PROTEIN 53 EXPRESSION
TO RESPONSE OF RADIATION THERAPY IN CERVICAL CANCER
Lydia Irtifany Lubis, Sarah Dina, Roy Yustin Simanjuntak, Hotma Partogi Pasaribu, Henry Salim Siregar, Riza Rivany
Obstetric and Gynecology Department
Faculty of Medicine of Sumatera Utara University
Medan, February 2017
Abstract
Objective: The aim of this study was to know the difference of protein 53 expression to response of radiation therapy in cervical cancer .
Methods: The analytics and case control study, conducted in the Obstetric and Gynecology Department, Faculty of Medicine of Sumatera Utara University, Adam Malik General Hospital and Pathology Anatomy Department, Adam Malik General Hospital started January until February 2017. We analyzed 15 paraffin-embedded blocks of cervical biopsy tissues with complete response as a control and 15 paraffin-embedded blocks with incomplete response as cases which has not been undergo the radiation therapy were collected consecutively and analyzed the p53 expression. Response to treatment was evaluated based on clinical examination and RECIST criteria and for apoptotic count was assessed by using Hematoxylin and eosin staining and was calculated apoptotic cell in 5 fields by used light microscopy. Kruskal – Wallis test performed to analyze statistically. Confidence interval is 83.5% and p value <0.05 is significance statistically.
Result: Total of 30 embedded that consists of 15 paraffin-embedded blocks with complete response and 15 paraffin-paraffin-embedded blocks with incomplete response. The stronger p53 expression were found in apoptotic count more than 5 apoptotic cells in 5 fields and p value = 0.033, and also the stronger p53 expression were found in incomplete response to radiation and p value = 0.046.
