Synthesis of N-4-methoxybenzoyl-N’phenylurea compound and activiy test of anticancer against HeLa cell line Repository - UNAIR REPOSITORY

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PHYTOCHEMICAL AND ANTIBACTERIAL ANALYSIS OF AQUEOUS AND ALCOHOLIC EXTRACTS OF VERNONIA AMYGDALINA (DEL.) LEAF

*Tsaku Alumbugu Paul, Ibrahim Taibat, Ekeleme Ike Kenneth, Nkenne Istifanus Haruna, Oti Victor Baba and Abimiku Rejoice Helma

ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11567 ]

DETECTIONS OF SOME RESPIRATORY VIRUSES BY MOLECULAR TECHNIQUES AMONG TWO SUDANESE TARGETS INDIVIDUALS

Ibrahim H. S.*, Kafi S. K., Musa H. A., Karsany M. S. and Abdel Hamid M. M. ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11543 ]

POLYUNSATURATED FATTY ACIDS INTAKE AND ITS ASSOCIATION WITH BREAST CANCER AMONG PATIENTS ATTENDING THE RADIATION AND ISOTOPE CENTER IN KHARTOUM STATE

Hafsa A. Elsafi* and Hassan A/Aziz Musa

ASSESSMENT OF CHRONIC KIDNEY FAILURE PATIENTS' AWARENESS ABOUT DISEASE AND HEMODIALYSIS COMPLICATIONS IN ALHASA REGION OF SAUDI ARABIA

Dr. Amal Khaleel Abu Alhommos* and Fahad Sami Al-Fahaid ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11644 ]

EVALUATION OF RISK FACTORS IN BREAST CANCER AMONG IRAQI FEMALE PATIENTS

Bushra Hasan Hashim*, Khulood Kareem Abed, Lina Ziyad Tareq, Arwa Ra’id Mishaal and Haneen Sedqi Ismael

SYNTHESIS OF N-4-METHOXYBENZOYL-N'-PHENYLUREA COMPOUND AND ACTIVITY TEST OF ANTICANCER AGAINST HeLa CELL LINE

Bambang Tri Purwanto*

DOCKING, SYNTHESIS AND CYTOTOXIC TEST ON HUMAN BREAST CANCER CELL LINE T47D OF N- (PHENYLCARBAMOTHIOYL)-BENZAMIDE

Dini Kesuma*, Siswandono, Bambang Tri Purwanto and Marcellino Rudyanto ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11474 ]

ASSESSMENT OF CINNAMON EFFECT TO REDUCE PRIMARY DYSMENORRHEA AND IMPROVE QUALITY OF LIFE AMONG FEMALES IN EASTERN REGION OF SAUDI ARABIA: A CROSS SECTIONAL SURVEY

Dr. Amal Khaleel Abu Alhommos* and Muneerah Al-Hadi ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11682 ]

ASSESSMENT OF SAUDI WOMEN AWARENESS ABOUT PAIN RELIEF AGENTS IN LABOR IN AL-HASA CITY OF SAUDI ARABIA

Dr. Amal Khaleel Abu Alhommos* and Malak AlDossary

2018- VOLUME 7, APRIL ISSUE 7

Research Article

World Journal of Pharmaceutical

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ANALYSIS OF PRESCRIPTION PATTERN OF ANTIBIOTICS USED FOR THE MANAGEMENT OF URINARY TRACT INFECTION IN A KIDNEY CENTER

Dr. Maqsood Ahmed Khan*, Syed Imran Ali, Sadia S. Kashif, Dr. Rabia Bushra, Aqsa Fatima Wali, Aiman Ashraf, Summaiya Mukhtar, Maryam Nasar, Aruba Hassan and Saba Shahid

PROTECTIVE ROLE OF MORINGA OLEIFERA LEAF EXTRACT AS A NATURAL ANTIOXIDANT AGAINST TOXIC EFFECT OF PARACETAMOL ON MALE MICE

Samia A. El-Fiky, Nagwa A. Hassan, Hassab El-Nabi S. E., Hasnaa A. Radwan* and Elsayad R. I. ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11581 ]

LOCAL SURVEY FOR AWARENESS OF HYPERACIDITY AND/OR OTHER ASSOCIATED PROBLEMS

Dr. Geeta D. Parulkar*, Dr. Manjushri Kharat, Dr. Abhishek Taksale, Dr. Ajitkumar Sahu, Dr. Vaishali Kokani, Dr. Ganesh Patare and Dr. Minal Bhusewar

CHARACTERIZATION OF A NOVEL TAYLOR-COUETTE ULTRAVIOLET REACTOR FOR NON-THERMAL PASTEURIZATION OF MILK

Rawa Abdul Redha Aziz* and Keith Warriner

TO EVALUATE GANDHRVAHASTYADI CHURNA IN MOOTRASHMARI

*Vd. Santosh Yashwant Solaskara nd Dr. Umesh Avinash Vaidya

TOTAL PHENOLIC CONTENTS OF SOME SEAWEED COLLECTED FROM RAIGAD COASTS OF KONKAN (MS)

J. S. Ambhore and *Whankatte V. R.

CHOLINE CHLORIDE-UREA: AN EFFICIENT, GREEN MEDIA FOR PARTIAL HYDROLYSIS OF DI- AND TRI-PROTECTED GANCICLOVIR AND SELECTIVE O-ACYLATION IN THE SYNTHESIS OF ANTIVIRAL DRUG VALGANCICLOVIR

Anita Goswami-Giri* and Pranaya P. Dhawle

ASSESSMENT OF QUALITY OF LIFE IN ADULT DIABETIC PATIENTS: PHARMACOLOGICAL THERAPY AND NON PHARMACOLOGICAL THERAPY

G. Prasanth*, R. Yogananda and N. J. Suba Sree

SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF THIADIAZOLE DERIVATIVES

*Dr. Rashmi Kumari, Dr. Vachaspati Dubey, Dr. S. K. Mishra and Anshu Raj ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11417 ]

FORMULATION AND EVALUATION OF FLOATING PULSATILE TABLET IN TABLET DRUG DELIVERY SYSTEM FOR HYPERTENSION

Mansi Shah* and Dipti Patel

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N. Vijaya Lakshmi*, V. Sandhya Reddy, S. K. Johny Basha, S. K. Shakeela Begum and D. Pavana Deepthi

SCREENING OF PHYTOCHEMICALS, ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF ELEPHANTOPUS SCABER LEAVES

Abarna N., Stephy Verghese and Jasmine R.*

MICROPROPAGATION OF AN ENDANGERED TERRESTRIAL ORCHID GEODORUM DENSIFLORUM (LAM.) SCHLTR. OF KANYAKUMARI DISTRICT, INDIA

G.V. Gegi, Dr. B. Christudhas Williams & Dr. R. Mary Suja ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11520 ]

SCREENING OF RHIZOMICROFLORA ISOLATED FROM THE RHIZOSPHERE OF AEGLE MARMELOS FOR MULTIPLE PLANT GROWTH PROMOTING AND ANTIMICROBIAL ACTIVITIES

Damle N. R.* and S. W. Kulkarni

SCREENING OF FUNGAL PATHOGENS FOR BIOLOGICAL MANAGEMENT OF NOXIOUS WEED EICHHORNIA CRASSIPES

R. P. Mishra and Sohni Jain*

EVALUATION OF ANTIMICROBIAL ACTIVITY OF EXTRACTS OF PIPER BETEL LEAVES AGAINST SOME COMMON PATHOGENS

V. P. Sutar*

SYNTHESIS AND CHARACTERIZATION OF NICKEL OXIDE BY USING UV/VIS SPECTROMETRY, SCANNING ELECTRON MICRSCOPY AND ITS ANTIMICROBIAL ACTIVITY AGAINST BACILLUS SPP., PSEUDOMONAS SPP. ESCHERICHIA COLI

J. C. Pradeep Kumar* and Dr. F. V. Dandawate

NON INVASIVE ASSESSMENT OF NUTRITIONAL STATUS AMONG SCHOOL CHILDREN OF A SEMI-URBAN AREA IN TAMILNADU

Suja Pandian R.*

CROSS MATCHING TRIBAL ETHNO MEDICINAL PRACTICES IN DIARRHEA & DYSENTERY BY BHIL- MINA TRIBE OF BANSWARA, RAJASTHAN WITH PUBLISHED PHARMACOLOGICAL RESEARCH

Dr. P. K. Dam* and Pankaj Kumar

EFFECT OF BIOCHAR AMENDMENT AND AGEING ON SORPTION AND DISSIPATION OF TETRACYCLINE’S IN SOILS

*Dr. O. P. Bansal

KNOWLEDGE AND AWARENESS ABOUT PHARMACOVIGILANCE AMONG 2ND YEAR MBBS STUDENTS AT GOVERNMENT MEDICAL COLLEGE AND HOSPITAL

Dr. Rajat Mishra*, Syed Meraj Fatmi and Dr. Sunil Kumar Singh ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11557 ]

EFFICACY OF JALANETI AND PRANAYAMA IN MANAGEMENT OF VATAJA PRATISHYAYA (ALLERGIC RHINITIS)

Dr. Manish Arora*, Dr. Patwardhan Ravindra, Dr. Shrikant G. Ingole and Dr. Rahul Gujarati ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11559 ]

CLINICAL EVALUATION OF BILVADI CHURAN IN THE MANAGEMENT OF GRAHANI ROG W.S.R. TO IBS (IRRITABLE BOWEL SYNDROME)

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IN VIVO STUDIES & SEM OF CONTROLLED POROSITY OSMOTIC PUMP (CPOP) TABLET OF ATENOLOL

Ayesha Sultana* and D. Varun

DEVELOPMENT AND VALIDATION OF AN UVSPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF TENELIGLIPTIN AND METFORMIN

Leena Ajay Sawaikar*

DEVELOPMENT OF POLYHERBAL PREPARATION FOR HAIR GROWTH PRMOTING ACTIVITY

Roshni Sahu*, Narendra Vyas, Shivendra S. Raghuwanshi, Sapna Malviya and Anil Khariya ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11570 ]

STUDY AND DESIGN OF BENZAMIDE AND PYRIDINECARBOXAMIDE DERIVATIVES AS A GLUCOKINASE ACTIVATOR

Dhanraj Patidar*, Sailesh Narayan, Rajeev Malviya and Phool Singh Yaduwanshi ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11574 ]

PURIFICATION OF BETA GALACTOSIDASE ENZYME FROM DAIRY EFFLUENT BACILLUS SPECIES

V. Pavithra and *Dr. J. Thirumagal

NEW AND EFFICIENT GREEN CATALYST ONE-POT SYNTHESIS OF BENZIMIDAZOLE AND DERIVATIVES

Gurumeet C. Wadhawa*, Vitthal S. Shivankar, Yashwant A. Gaikwad, Nilam S. Dhumale, Charansingh H. Gill and Laxman V. Gavali

IN VITRO ANTICANCER ACTIVITY OF ETHANOLIC LEAF EXTRACT OF Acampe praemorsa (Roxb.)

G. Soumiya*, Dr. B. Christudhas Williams and Dr. R. Mary Suja ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11585 ]

ANTIBIOTIC SUSCEPTIBILITY PATTERN OF EXTENDED SPECTRUM Β-LACTAMASE PRODUCING ESCHERICHIA COLI

Parveen W.* and Subhashini A.

PHYTOCHEMICALS AND ANTIOXIDANT ACTIVITIES OF APPLE BER, A HYBRID VARIETY OF ZIZIPHUS MAURITIANA

Rajni Nigam*

FORMULATION AND EVALUATION OF GELRITE BASED PHASE CHANGE OPHTHALMIC SOLUTION OF NEPAFENAC- As QBD APPROACH

Deepak G. Wagh* and Dr. Sadhana R. Shahi

SCREENING FOR CONGENITAL HYPOTHYROIDISM IN GREATER NOIDA BY MEASUREMENT OF UMBILICAL CORD THYROXINE (FREE) AND THYROID STIMULATING HORMONE: A MULTICENTRIC HOSPITAL BASED STUDY

Chandra Prakash Sharma, Widhi Dubey and Dr. Suryakant Nagtilak* ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11632 ]

IN-VITRO ANTIHELMINTHIC ACTIVITY, PHOTO CHEMICAL SCREENING AND TLC STUDIES OF METHANOL EXTRACTION ON CELOSIA CRISTATA FLOWER USING IN - STATE FESTIVAL OF TELANGANA (BATHUKAMMA)

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ANTIBACTERIAL SCREENING OF AMORPHOPHALLUS CAMPANULATUS ROXB. AGAINST SELECTED HUMAN PATHOGENIC BACTERIA

Dr. T. Francis Xavier, G. Dhanasekaran* and D. Sathish Kumar ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11636 ]

ISOLATION AND MOLECULAR IDENTIFICATION OF HALOPHILIC ARCHAEA (Part –I) FROM SALINE SOIL

Arpita R. Dave* and Dr. Ajit V. Pandya

GREEN SYNTHESIS OF GOLD NANOPARTICLES USING CASSIA ALATA LEAF EXTRACT AND EVALUATION OF ANTIMICROBIAL ACTIVITIES

Le Thi Thanh, Thejesh Kumar M. P. and Rajkumar H. Garampalli* ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11640 ]

N-LINKED GLYCOSYLATION AND CHARGE VARIANT ANALYSIS OF BIOSIMILAR ANTI HER2 ANTIBODY IN TREATMENT OF BREAST CANCER

Pratik R. Gajjar*, Dasharath M. Patel, Amar K. Shrivastava, Umesh S. Shaligram, Noelle Sunstrom ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11645 ]

GC-MS AND ANTIOXIDANT POTENTIAL OF METHANOLIC LEAF EXTRACT OF PUTRANJIVA ROXBURGHII WALL. (PUTRANJIVACEAE)

Emasushan M.* and John Britto S.

ISOLATION AND IDENTIFICATION OF XYLANASE PRODUCING MICROORGANISMS FROM SOIL

Rajakumar R.* and Rameshwara Reddy Naru

DESIGN AND PHARMACOLOGICAL STUDIES OF NEW COUMARIN DERIVED CHALCONE SYNTHESIZED COMPOUNDS

Sai Chandra J.*

A NOVEL VALIDATED STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATIONOF NALOXEGOL

Y. Sirisha* and Anilkumar Y.

STUDY OF THYROID FUNCTIONS IN TYPE 2 DIABETES MELLITUS AND RELATIONSHIP WITH OBESITY IN A TERTIARY CARE HOSPITAL, BANKURA, WEST BENGAL

Dr. Sumanta Banerjee*, Dr. Santa Saha (Roy), Dr. Pinaki Sarkar, Dr. Biswanath Sharma Sarkar and Dr. Dibakar Haldar

A COMPARISON OF VESTIBULAR REHABILITATION APPROACHES ON BALANCE IN HEARING IMPAIRED CHILDREN WITH VESTIBULAR DYSFUNCTION

Shilpa Khandare, Nigel Gonsalves*, Dr. Tushar Palekar PhD., Vidhi Shah and Trupti Siddapur ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11669 ]

VALIDATION OF STABILITY INDICATING HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF ASSAY OF CARBAMAZEPINE DRUG IN THE PHARMACEUTICALS TABLET FORMULATIONS USING PHENYTOIN AS AN INTERNAL STANDARD

Shaikh Javed Shaikh Afzal*, Suresh C. Ameta and Pathan Mohd Arif Ali Khan ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11670 ]

STUDY OF POLYPHARMACY IN GERIATRIC PATIENTS IN A TERTIARY CARE HOSPITAL: A PROSPECTIVE OBSERVATIONAL STUDY

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CHARACTERIZATION OF SALMONELLA SPP– PRODUCING EXTENDED-SPECTRUM Β-LACTAMASE (ESBL) ISOLATED FROM CHICKEN SLAUGHTERHOUSES PROCESSING LINE IN NAMAKKAL

*R. Sowmiyadevi, M. Anitha, D. Jegadeeshkumar and B. Madhumathi ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11677 ]

AN ASSESSMENT OF GROUND WATER QUALITY IN RURAL AREA OF ARAVALLI DISTRICT IN WINTER SEASON BY PHYSICOCHEMICAL PARAMETERS

Preksha Patel, Shiv Singh Dulawat* and Mangal Shree Dulawat ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11680 ]

SYNTHESIS AND ANTIMICROBIAL EVALUATION OF SOME SUBSTITUTED-BENZOYL HYDRAZINO DERIVATIVES OF PYRIMIDINE

Darshitkumar M. Dave* and Dr. A. H. Bapodra

EFFICACY OF KANTAKARI MARICHA LEHA ON KAPHAJA KASA IN CHILDRENOF AGE GROUP 1 TO 5 YEARS

Dr. Shital Sawant*, Dr. J. A. Nandgaonkar and Dr. Rahul H. Gujarathi ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11693 ]

CHEMOMETRICS ASSISTED SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF ALOGLIPTIN AND METFORMIN IN PHARMACEUTICAL DOSAGE FORM

Premakumari K. B.*, Sandhyavali M. S., Murugan V. and Shanaz Banu ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11697 ]

ASSESSMENT OF BIOMOLECULES FROM LEAVES EXTRACT OF ETHNIC PLANTS

B. D. Nagle, Anil Kumar*, K Khasdeo, S. R. Gayakwad, A. D. Lazrus ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11698 ]

EXPLORATION OF ACTINOBACTERIA FROM MANGROVE ECOSYSTEM OF KRISHNA DISTRICT, ANDHRA PRADESH

Krishna Naragani*

METABOLIC SYNDROME AND RISK OF CARDIOVASCULAR DISEASE IN RURAL AND URBAN PATIENTS IN NORTH INDIA

Manoj Kumar Sharma, Sonali Pandey and Suryakant Nagtilak* ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11707 ]

METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC FOR SIMULTANEOUS ESTIMATION OF ETODOLAC & THIOCOLCHICOSIDE

N. Sai Prudhvi*, M. Prasadarao and G. Anil Kumar

POTENTIAL USE OF WILD GANODERMA SP BASED PETROLATUM PASTE AGAINST SOME MIXED DERMATOLOGICAL CONDITIONS IN GOATS IN MAIDUGURI, NIGERIA

*Shamaki B. U., Sandabe U. K., Abdulrahman F.I., Sa’idu S. N. A., Tekdek L. B. and Ya’uba A. M. ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11716 ]

SYNTHESIS OF GOLD NANOPARTICLES FROM NATURAL HONEY AND ITS APPLICATION IN ANTIFUNGAL ACTIVITY

Dr. R. Manju* and D. Savi

FORMULATION AND EVALUATION OF NANOPARTICLES OF EPROSARTAN MESYLATE

Nihar Ranjan Kar* and Manisa Patel

PREVALENCE OF COLOR BLINDNESS AMONG THE STUDENTS OF RIYADH COLLEGES OF DENTISTRY AND PHARMACY

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ASSESSMENT OF THE KNOWLEDGE, ATTITUDE, AND PRACRTICE OF SUDANESE MOTHERS TOWARD CHILDHOOD VACCINATION, AT KHARTOUM NORTH HEATH CENTERS: SUDAN

Tahany Mohamed Osman Mohamed and *Kamal Addin Mohammad Ahmad Idris ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11739 ]

ASSESSMENT OF THE KNOWLEDGE, ATTITUDE AND PRACTICE OF SUDANESE ADOLESCENT SECONDARY SCHOOL STUDENTS ABOUT THE APPROPRIATE AND SAFE HANDLING OF MEDICINES

Sahar Mohamed Osman Mohamed and Kamal Addin Mohammad Ahmad Idris* ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11740 ]

ASSESSMENT OF IN-PATIENTS’ SATISFACTION WITH THE QUALITY OF HEALTH CARE SERVICES PROVIDED IN SOBA UNIVERISTY TEACHING HOSPITAL, KHARTOUM, SUDAN

Fatima Ismail Hussein Badri and *Kamal Addin Mohammad Ahmad Idris ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11741 ]

EVALUATION OF IN VITRO CYTOTOXICITY OF DIMETHOATE ON HUMAN PERIPHERAL BLOOD LYMPHOCYTES USING MTT ASSAY

Dr. Daya Shankar Gautam*, Meenal Raikwar, Roshni Soni and Sheeba Begum ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11773 ]

SCREENING OF PRE-HYPERTENSION RISK FACTORS AMONG TEACHING PROFESSIONALS

Nigel Gonsalves*, Amita Aggarwal, Dr. Tushar J. Palekar PhD. and Chetna Jadhav ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11775 ]

DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATION OF ROSUVASTATIN AND EZETIMIBE IN CAPSULE DOSAGE FORM

Patel Aesha A.* and Dr. Paresh Patel

HEPATOPROTECTIVE ACTIVITY OF METHANOLIC EXTRACT OF GRACILARIA CORTICATA J.AG. IN HARE ISLAND, THOOTHUKUDI, TAMIL NADU, INDIA

*John Peter Paul J. and Raja P.

MONONUCLEAR LINEAR-SHAPED ASSEMBLIES OF N,N’-DIETHYLBENZIMIDAZOLYDINE BASED IONIC LIQUID COORDINATED TO AG(I)-AND CU(I)-NHC AG(I)-AND THEIR ANTIBACTERIAL AG(I)-AND ANTIOXIDANT APPLICATIONS

A. G. Bharathi Dileepan, R. Mathumidha, D. Maruthamuthu, R. Ranjith, S. Priscilla Prabavathi and Shameela Rajam*

RESIDUE DEPLETION OF TILMICOSIN IN CHICKEN TISSUES

Abdelrahman Mostafa*, Nuha M. A. Agbna, Samia A. Wahab and Sania Shaddad ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11046 ]

ETHANOBOTANICAL CHARACTERIZATION OF COCCINIA INDICA AGAINST URINARY TRACT INFECTION

Deepa M. and Sharly Elgal N*.

EVALUATION OF ANXIOLYTIC ACTIVITY OF SUBSTITUTED 1, 2, 4-TRIAZOLE BEARING IMINO, FIVE MEMBERED HETEROCYCLIC MOIETY

D. Kumudha*, T. Kalavathi and B. A. Viswanath

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AN OBSERVATIONAL STUDY ON THE ASSOCIATION OF LOW BACK PAIN AND MID-LINE FISSURE IN TONGUE

R. Saranya*, G. J. Christian, S. Elansekaran and M. Ramamoorthy ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11477 ]

EVALUATION OF IN VITRO ANTIOXIDANT PROPERTIES OF ETHANOLIC AND AQUEOUS EXTRACTS OF ACHYRANTHES ASPERA L.

Thafshila Aafrin A. M. and Anuradha R.*

GREENER APPROACH OF SYNTHESIS OF AZO DERIVATIVES AND BIS- PYRAZOLE DERIVATIVES ALONG WITH ANTIMICROBIAL SCREENING

Prashant P. Chaudhari* and Shankarsing S. Rajput

COMPARATIVE PHYTOCHEMICAL AND SPECTROPHOTOMETRIC ANTIOXIDANT EVALUATION OF THE ROOT BARK, STEM BARK, LEAF AND SEED OF COLA NITIDA (STERCULACEAE)

Ukwueze Stanley Ejike* and Ugwu Nneka Njideka

ANTIPYRETIC EFFECT OF ORAL VERSUS RECTAL ACETAMINOPHEN IN CHILDREN - A RANDOMIZED COMPARATIV STUDY

Nobo Krishna Ghosh, Farhana Tanzin, Forhad Manjur, Farhana Rizwan, Morsheda Khanam and S. M. Shatil Shahriar

ESTABLISHMENT OF RICE HUSK BY-PRODUCT AS PHARMACEUTICAL EXCIPIENTS

Anil Kumar*, Durga Prasad Patel*, Gajendra Kumar Patel, Deepika Singh, Anand Kumar Prasad and Dr. Khomendra Sarva

IN-VITRO CONSERVATION OF MOMORDICA CYMBALARIA HOOK. F.

S. V. Madhale* and N. S. Chavan

HALALI RESERVOIR RESTUDY THE SEASONAL VARIATIONS IN PHYSICO-CHEMICAL CHARACTERISTICS OF WATER

Dr. Reena Yadav* and Dr. Pramod Patil

A STUDY ON ANTI-PUTREFACTION PROPERTY OF DODONAE ANGUSTIFOLIA

J. K. Alphonsa Juliet Helina* and A. Peter Pascal Regis ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11761 ]

OXIDATIVE HYDROXYLATION OF OMEPRAZOLE IN HEALTHY SUBJECTS OF NIGER DELTA REGION BY THIN LAYER CHROMATOGRAPHY

Benjamin U. Ebeshi*, Vaikosen N. Edebi and Jonathan O. Onajemo ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11764 ]

MATERNAL RISK FACTORS- AN OBSERVATIONAL STUDY ON DEMOGRAPHIC VARIABLES FOR NEONATAL CONJUCTIVITIS

Dr. Rahul Ghoti* and Dr. Rahul H. Gujarathi

PREPARATION, CHARACTERIZATION AND CELL UPTAKE STUDIES OF MULTI-DRUG POLYMERIC NANOPARTICLES LOADED PATCHES FOR MANAGEMENT OF AIDS AND TUBERCULOSIS IN PAEDIATRIC POPULATION

Mathur M., Raichur V. and V. Kusumdevi*

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P. S. Chinnasamy, S. Parimala and M. Kandhasamy* ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11770 ]

QUENCHING METHOD - A NOVEL TECHNIQUE IN THE FORMULATION AND EVALUATION OF SOLID LIPID NANOPARTICLES TAKING QUETIAPIN FUMARATE AS A MODEL DRUG

P. Tripura Sundari* and Hari Anushree

QUALITY PARAMETERS FOR STANDARDIZATION OF FICUS RELIGIOSA LINN. STEM BARK: AN UPDATE

Shiva and Sumitra Singh*

USE OF DRAGENDORFFS REAGENT FOR THIN LAYER CHROMATOGRAPHIC DETECTION OF TRIAZINE CLASS HERBICIDE ATRAZINE

Umakant D. Pawar*, Chandrakant D. Pawar, Ulka K. Kulkarni, Devanand B. Shinde and Rajendra K. Pardeshi*

PREVALENCE OF HBeAg IN CHRONIC HEPATITIS B PATIENTS IN KHARTOUM, SUDAN

Shamsoun Kafi, Abosufyan Salama* and Sarra Alfadil ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11793 ]

MOLECULAR STUDY OF SLC2A9 GENE IN DIABETIC ASTHENOZOOSPERMIC IRAQI PATIENTS

Sara Harith Abdul-Razaaq* and Prof. Dr. Waleed Hameed Yousif ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11796 ]

SIMULTANEOUS ESTIMATION OF LEVOSULPIRIDE AND ILAPRAZOLE IN CAPSULE DOSAGE FORM BY SIMULTANEOUS EQUATION SPECTROPHOTOMETRIC METHOD AND QABSORBANCE RATIO METHOD

*Devanshi H. Rami and Dr. Sejal K. Patel

TO STUDY THE PREVALENCE OF STRESS IN SCHOOL GOING CHILDREN AGED 10-16 YEARS OLD

Dr. Shilpa Khandare*, Sanjana Chakrabarty, Dr. Preeti Gazbare, Dr. Mayura Deshmukh, Dr. Tanpreet Kaur Bagga and Dr. Tushar Palekar

USES OF LEAVES IN TRADITIONAL MEDICINE IN AURANGABAD DISTRICT (MAHARASHTRA) INDIA

I. H. Zahid and *Rafiuddin Naser

OPTIMIZATION STUDIES ON ALPHA AMYLASE PRODUCTION BY BACILLUS LICHENIFORMIS DS3 AND BACILLUS SUBTILIS DS7 USING SUBMERGED FERMENTATION

D. Silpa, P. Brahmaji Rao* and G. Kranthi Kumar

SPECTROPHOTOMETRIC AND RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF LEVOFLOXACIN

Kauser Fatema*, Sadhana Shahi, Tauqeer Shaikh and Nityanand Zadbuke ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11821 ]

ACIDIC CHITINASE PRODUCTION BY NEISSERIA SPECIES

R. S. Mane*, G. S. Patil and B. R. Choradiya

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DESTAINING POTENTIAL OF BACTERIAL LIPASE ENZYME ISOLATED FROM PROVIDENCIA RETTGERI INHABITING THE FRESH WATER FISH MYSTUS BLEEKERI

N. Deepa, R. Usha, A. Anbarasu, K. Anuanandhi, P. Karnan and K. Elumalai* ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11855 ]

OPTIMIZATION AND EVALUATION OF TELMISARTAN FAST DISSOLVING TABLETS EMPLOYING STARCH GLUTARATE -A NEW SUPERDISINTEGRANT

*A. Bharathi and D. Chandra Sekhar Naik

DEVELOPMENT OF WHEY BASED PROBIOTIC MANGO BEVERAGE

Lakshmikanth A. V.*, Dr. D. B. Puranik and Soumya Shree T. C. ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11884 ]

PLANT PATHOLOGY: A NEW HORIZON

Dr. Teena Agrawal*

ANTICANCER ACTIVITY AND ANTI DIABETIC ACTIVITY OF STREPTOMYCES SPECIES JKCM1

*M. Guravaiah

SPECIES OF ALTERNARIA WERE REPORTED FIRST TIME FROM HATAIKHEDA DAM OF BHOPAL (M.P.)

Kusum Lata*, Dr. Aparna Alia, Dr. Pramod Patil, Dr. Ranjana Singh ABSTRACT PDF [ DOI : 10.20959/wjpr20187-12663 ]

PHYTO ANALYSIS AND COMPARATIVE ESTIMATION OF TFC RAUVOLFIA SERPENTINA QUALITY PARAMETERS IN MARKETED AND FOREST SAMPLES.

Rahnuma Khan*, Dr. Manish Mishra and Dr. Mukta Shrivastav ABSTRACT PDF [ DOI : 10.20959/wjpr20187-12664 ]

PHYTO ANALYSIS AND COMPARATIVE ESTIMATION OF TPC IN EMBLICA OFFICINALIS QUALITY PARAMETERS IN MARKETED AND FOREST SAMPLES.

Rahnuma Khan*, Dr. Manish Mishra and Dr. Mukta Shrivastav ABSTRACT PDF [ DOI : 10.20959/wjpr20187-12665 ]

COMPARATIVE ANALYSIS OF PHYSICO-CHEMICAL PROPERTIES OF WATER OF FIVE LAKES OF BHOPAL, INDIA

P62 CROSSTALK IN CANCER THROUGH SELECTIVE AUTOPHAGY

Md. Ariful Islam and Pinghu Zhang*

ABSTRACT Article View (10) Article Download (4) [ DOI : 10.20959/wjpr20187-11531 ]

THE EARLY INFANTILE EPILEPTIC ENCEPHALOPATHY ASSOCIATED WITH MUTATION IN GABRB3 GENE

T.V. Kozhanova*, S.S. Zhilina, T.I. Mescheryakova, E.G. Lukyanova, E.S. Bolshakova, K.V.Osipova, S.O. Ayvazyan and A.G. Prityko

ALGAE IN THE BIOTIC ASSOCIATIONS

*Dr. Teena Agarawal

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DISCOMYCETES OF THE ASCOMYCETES GROUP

*Dr. Teena Agarwal

ROLE AND OVERVIEW OF DRUG REGULATORY AFFAIRS IN PHARMACEUTICAL INDUSTRY WITH IMPLEMENTATION OF CTD AND ECTD

Sonali P. Mahaparale* and Bhakti R. Desai

B. RATHINA NAICKER & SONS – ONE OF THE PIONEERS IN SIDDHA LITERATURE PUBLICATION

R. Manickavasagam*, C. Senthamil Rajam and B. Purosothaman

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Prajapati Anil*, Urvashi Sharma, Muley Preeti, Malviya Sapna and Kharia Anil

RECENT ADVANCE IN ANTI-CANCER ACTIVITY OF INDOLE DERIVATIVES

C. Buvana*, R. Suresh, Y. Haribabu and P. K. Manna

TRANSDERMAL DRUG DELIVERY SYSTEM: A REVIEW

Anchal Sharma*, Rajeev Garg, L. Raju and Sachin Goyal

DRUG DELIVERY THROUGH NAILS

Patil P. B.*, Gadkari P. N. and Saudagar R. B.

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Rakshith U. R.*, Balaji M. N. and M. Ramesh

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DIARRHOEA IN CHILDREN AND ITS AYURVEDIC MANAGEMENT: A REVIEW

Vd. Promila Mohar Singh Thakur* and Vd. V. U. Gawai

NANOROBOTS A FUTURE DEVICE FOR DIAGNOSIS AND TREATMENT

Sarath Kumar S.*, Sneha Sabu, Anjana S., Alfiya Ali, Dr. Beena P. Nasim and Dr. Elessy Abraham ABSTRACT Article View (24) Article Download (7) [ DOI : 10.20959/wjpr20187-11521 ]

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Heta K. Patel* and Dr. Dhiren P. Shah

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TRANSDERMAL DRUG DELIVERY SYSTEM: A REVIEW

Akshay H. Bhoye*, AbdulKhalique A. Shaikh, Dr. Dhiren P. Shah and Tejas J. Patel

A RECENT REVIEW ON INTRANASAL MICROEMULSION: A NOVEL DRUG CARRIER SYSTEM

Halde B. R.*, Darekar A. B. and Saudagar R. B.

OVER REVIEW OF NASAL IN SITU GELS

P. Jagadeesh*, P. Jyothi, M. Bhargav, T. S. Nazma, M. Neeraja, N. Pushpalatha, T. Sharath Babu, S. Shamshad and B. Yellasivamma

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SKIN AND ACNE RELATED ISSUES AMONG GROWING CHILDREN

Mangwal Ketan, Sharma Pragya and Mishra Dhruv*

STUDY ON REGULATORY REQUIREMENTS OF SAFETY REPORT IN US, EUROPE, JAPAN AND INDIA

Dr. Dilip G. Maheshwari* and Ramprakash G. Parmar

A REVIEW ON BIOFILM MEDIATED BIOREMEDIATION

Shweta Nakul* and Nisha Kanwar

LARGE SCALE PRODUCTION OF MICRO ALGAE AND EXTRACTION OF BIOOIL BY TRANS ESTERIFICATION METHOD

G. Rajkumar and *Dr. J. Thirumagal

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Gurumeet C. Wadhawa*, Vitthal S. Shivankar, Yashwant A. Gaikwad Charansingh H. Gill and Laxman V. Gavali

SHADA-AGRYA: PRE-EMINENT FACTORS FOR PROTECTION OF THE HEART

Vd. Yadav Varsha Jagannath* and Dr. Madhuri A. Pachghare

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Dr. Onkar V. Hanashi*, Dr. Mahantesh B. Rudharapuri and Dr. Vinay Mohan

PHARMACEUTICAL CRIME: A REVIEW

Deepanshu Gupta*, Chandrakanta Kushwah, Ankur Joshi, Sapna Malviya and Anil Kharia ABSTRACT Article View (21) Article Download (4) [ DOI : 10.20959/wjpr20187-11611 ]

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CONCEPT AND MANAGEMENT OF SCABIES (JARB) IN UNANI SYSTEM OF MEDICINE

Abdul Nasir*, Gazala Fatma and Mohd Akhtar Siddiqui

BIOSENSORS AND ITS APPLICATIONS

Roshni B. Nair*

REVIEW ON HERBAL COSMETICS

Saudagar R. B.* and Sisodiya M. H.

CHILDHOOD MENTAL DISORDER AND COMPLEMENTARY MEDICINE: A REVIEW STUDY

Dr. Neha Udainiya* and Dr. Nitin Sharma

EFFECT OF VIRECHANA KARMA AND ORAL AYURVEDIC TREATMENT IN THE MANAGEMENT OF PADASHOTH DUE TO VARIOSE VEINS

*Vd. Sunil A. Bhaskare and Vd. Madhuri S. Jadhav

REVIEW ON PELLETIZATION: TECHNIQUES, CHARACTERIZATION AND APPLICATIONS

Harshada Dattatraya Dalvi*, Dr. Nilesh Khutle, Priyanka Pawar and Abhijeet Kunwarpuriya ABSTRACT Article View (23) Article Download (12) [ DOI : 10.20959/wjpr20187-11668 ]

COMPREHENSIVE REVIEW OF SROTAS IN AYURVEDA AND ITS IMPORTANCE IN SHARIR

Dr. Sona Rani*, Dr. Sunil Kumar Yadav and Dr. Jaivardhan Singh

A REVIEW ARTICLE ON PATHO-CLINICAL STUDY ON DISORDERS OF MUTRAVAHA SROTAS & TYPES OF MUTRASHMARI

Dr. Ashish Arun Madavi* and Dr. R. H. Amilkanthwar

BAYLIS-HILLMAN REACTION IN ORGANIC CHEMISTRY

Dandamudi V. Lenin*

ROLE OF SADVRITT AND AACHAR RASAYANA IN PSYCHOSOMATIC DISEASES

Shekhawat Suman*, Mishra Pramod Kumar, Soni Anamika, Sharma Brahmanand, Singh Balraj ABSTRACT Article View (17) Article Download (3) [ DOI : 10.20959/wjpr20187-11690 ]

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*Dr. Mangal Singh, Dr. Chandan Singh, Dr. Jyoti Dhakar, Dr. Surendra Kumar Verma and Dr. Jayanat Nagar

SYNTHESIS OF CHITOSAN-POLYANILINE-TIO2 POLYMER BASED NANO PARTICLES AND THEIR CHARACTERIZATION

R. Ranjith, *Dr. Shameela Rajam and R. Pandiyan

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KAUMARBHRITYA AS A PREVENTIVE PEDIATRICS FOR CHILDHOOD DISEASES

Dr. Dattatrya G. Parde*, Dr. Laxmikant G. Thakare and Dr. Vidya S. Ingole

APPROACH IN AYURVEDA TO KEEP HEALTHY EYE – PREVENTIVE ASPECTS

Dr. Shailendra Singh*, Dr. Mayank Bhatkoti and Dr. Payal Thakur

LIFESTYLE DISORDERS AND THEIR PREVENTION THROUGH AYURVEDA

Dr. Ashwini Diliprao Ghuge* and Dr. V. E. Gogate

MANAGEMENT OF PAIN OF OCULAR AND ENT ORIGIN THROUGH AYURVED

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QUANTIFICATION OF IRINOTECAN (CPT-11) AND ITS METABOLITE, SN-38, IN RAT PLASMA AND BILE SAMPLES: APPLICATION TO PHARMACOKINETIC STUDIES

Ranjan Kumar Singh* and Narsingh Rajpoot

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF BEDAQUILINE IN BULK AND PHARMACEUTICAL FORMULATIONS

B. S. Pooja* and Dr. A Sathish Kumar Shetty

UREDINALES THE RUST FUNGI

Dr. Teena Agrawal*

NIOSOME: A NANO-TARGETED DRUG DELIVERY SYSTEM

Sadhana Noothi*

JATAMANSI (NARDOSTACHYA JATAMANSI): A COMPREHENSIVE REVIEW

*Dr. Ankita Goyal, Dr. Sudipt Rath and Dr. Sachin Mittal

MECHANISMS OF VANCOMYCIN RESISTANCE IN STAPHYLOCOCCUS AUREUS, ARTICLE REVIEW

Riad A. Dellol*

SOLUBILITY ENHANCEMENT OF POORLY WATER SOLUBLE DRUG BY USING VARIOUS SOLUBILITY ENHANCEMENT TECHNIQUES

Priyanka Hanumant Pawar*, Dr. Nilesh Khutle and Harshada Dalvi

RADIO-PROTECTIVE POTENTIAL AND ANTIMICROBIAL ACTIVITY OF PUDINA. (MENTHA SPP. /MINT)

Sadaf Shaikh and Mansi Shah*

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THE ROLE OF VIRECHAN KARMA AND INDIGENOUS DRUG WITH BAKUCHI-TUVRAK OIL IN THE MANAGEMENT OF SHVITRA W.S.R TO VITILIOGO

Dr. Himani Khajuria* and Dr. Monika Gupta

COLON TARGETED DRUG DELIVERY – A REVIEW ON PRIMARY AND NOVEL APPROACHES

Nehal M. Bhatt* and Rakesh P. Patel

PHARMACOLOGICAL EVALUATION OF PALASHBEEJADI YOGA AGAINST WORMS INFESTATION

Gupta Shilpy* and Kumar Vijendra

A REVIEW ON HERBAL APPROACH TOWARDS URTICARIA AN ALLERGIC SKIN DISORDER

Kushal Shah, Vedshree Raole, Namrata Parikh, Shachi Engineer, Ritav Bhrambhatt, Bhavik Chauhan*

ANTI-DIABETIC ACTIVITY OF REPORTED MEDICAL AND AROMATIC PLANTS: A REVIEW

Venugopala Reddy Mekala*

STANDARDIZATION OF MODIFIED DHUM NETRA

Dr. Hemangi B. Shukla*

PHARMACOLOGICAL EVALUATION AND ACTION OF KARANJA PATRA KALKA SIDDHA TAILA IN STRIAE GRAVIDARUM

Kumar Vijendra* and Gupta Shilpy

GENETIC AND ENVIRONMENTAL BASIS OF DISEASES: AN AYURVEDIC PERSPECTIVE

Dr. Gupta Shilpy* and Kumar Vijendra

MYXOMYCETE A AMAZING SLIME MOLDS GROUP

Dr. Teena Agrawal*

CHRONOTHERAPY: NEW APPROACH FOR TREATMENT

Dr. A. Bharathi*

METHOD DEVELOPMENT AND VALIDATION OF NORETHINDRONE BY UV-VISIBLE SPECTROPHOTOMETER IN BULK AND PHARMACEUTICAL DOSAGE FORM

Merugu Manasa*, P. Siva Kumar, N. Sahani, N. Sujatha and P. Sahiti

PHOTOTHERAPY: A MODERN EXTENSION OF SWEDANA CHIKITSA FOR NEONATAL JAUNDICE

Srivastava Mayank*

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A CASE REPORT ON DENGUE FEVER IN PREGNANT WOMEN

Bushra Saba*, Dr. Shaheda Siddiqui, Minhaj Sultana and Sana Rafeeq ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11506 ]

AYURVEDIC APPROACH IN RHEUMATOID ARTHRITIS (AMAVATA) A CASE REPORT

Dr. Jyoti Khandare*, Dr. V. E. Gogate and Dr. S. V. Suryavanshi ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11519 ]

MANAGEMENT OF ASCITES THROUGH AYUREDA: A CASE STUDY

*Dr. Upasna Gulati and Dr. Sanjay Kumar Tripathi

EFFECT OF AYURVEDIC FORMULATION ON SEASONAL RESPIRATORY DISEASE: A SINGLE CASE STUDY

*Vd. Manche Yogesh R., Vd. Prasad P. Deshpande and Vd. V. G. Patrikar ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11575 ]

ANU TAIL NASYA AND SHIRAH SHOOLADI VAJRA RASA IN THE MANAGEMENT OF ARDHAVABHEDAK ROGA (COMMON MIGRAINE) - A CASE STUDY

Ajay Kumar Pandey*, Sanjay Kumar Tripathi and Priyaranjan Tiwari ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11631 ]

DENGUE FEVER AND ATYPICAL KAWASAKI DISEASE: A RARE AND CHALLENGING COMBINATION

Pragnadyuti Mandal*, Ajay Kumar Shit, Alak Kumar Das, Mihir Sarkar and Gobinda Mondal ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11694 ]

A CRITICAL STUDY ABOUT DEMONETIZATION – ITS MERITS, DEMERITS AND IMPACTS ON THE SOCIETY

K. L. Rathi* and Touseef Hussain Trak

EFFECT OF NEEM DECOCTION IN PIT & FISSURE DENTAL CARIES- A SINGLE CASE STUDY

*Dr. Avneet Kaur Dr. Anilkumar Chauhan, Dr. Radhakrishan Biswal and Dr. Manu Sharma ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11705 ] AGANTUJA VRANA (POST INSECT BITE WOUND) - CASE STUDY

Dr. Kalpesh Dattani and Dr. Joyal Patel*

A CASE STUDY: MANEGMENT OF EKAKUSHTHA W.S.R. TO GUTTATE PSORISIS BY PANCHTIKTA GHRITA AND RAKTAMOKSHAN

Dr. Kailash Honde* and Dr. Raman R. Ghugralekar

A CASE REPORT ON DICLOFENAC INDUCED ERYTHEMA MULTIFORME

Dr. Bejjanki Amrutha*, Dr. C.N. Harshitha, Dr. Garsha Anusha and Dr. Andugula Kavya ABSTRACT PDF [ DOI : 10.20959/wjpr20187-11582 ]

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www.wjpr.net Vol 7, Issue 07, 2018. 56 Purwanto. _{World Journal of Pharmaceutical Research}

SYNTHESIS OF

N

_{-4-METHOXYBENZOYL-}

N

_'-PHENYLUREA

COMPOUND AND ACTIVITY TEST OF ANTICANCER AGAINST

HeLa CELL LINE

Bambang Tri Purwanto*

Departement of Pharmaceutical Chemistry, Faculty of Pharmacy, Airlangga University.

ABSTRACT

Research in the search for cancer drug compounds continues to be

developed, considering that specific anticancer compound has not been

obtained. Some of the urea derivative compounds are also being

developed in the search for an anti-cancer compound which is potent

with minimal side effects. Related to the explanation above, a urea

derivative, which is N-phenylurea, would like to be developed. It

would be reacted with 4-methoxybenzoyl chloride, thus

N-4-methoxybenzoyl-N'-phenylurea compound would be obtained. The

synthesis of N-4-methoxybenzoyl-N'-phenylurea was carried out by

Schotten-Baumman method that had been modified, and then a test of

purity was done by thin layer chromatography using 3 different kinds of eluent. The next

phase was structure characterization which was carried out by using UV and IR

spectrophotometry, then 1H-NMR spectrometry also MS, so that the structure from

N-4-methoxybenzoyl-N'-phenylurea would be obtained. Anticancer activation test was conducted

on HeLa cell line by using MTT assay, and the value of IC50 would be obtained. The

compound that has been successfully synthesized is N-4-methoxybenzoyl-N'-phenylurea,

with a yield of 51,23%. The purity test of N-4-methoxybenzoyl-N'-phenylurea was done by

thin layer chromatography with 3 different types of eluent (hexane: ethyl acetate: methanol =

2: 3: 1; Hexane: acetone = 4: 2; Hexane: ethyl acetate = 4: 2) one single spot was obtained,

which its value of Rf is different than the original compound, N-phenylurea. While the

melting point of the compound is 158oC, thus it was seen that the compound has been formed

and was different from the original compound, N-phenylurea. Anticancer activation test has

been performed with MTT assay method by using HeLa cell line, the result obtained of IC50

is 6.50 mM more active than hydroxyurea is 170.57 mM as a standard. The conclusion is that

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www.wjpr.net Vol 7, Issue 07, 2018. 57 N-4-methoxybenzoyl-N'phenylurea, have been successfully synthesized and has anticancer

activity.

KEYWORDS: synthesis; N-4-methoxybenzoyl-N'-phenylurea; anticancer activity.

INTRODUCTION

Cancer is one of the diseases which is the main cause of death in developing countries and

also worldwide. From 58 million worldwide death in 2005, there were 7.6 million (13%)

which were caused by cancer. Death which is caused by cancer was expected to increase in

2015 and 11.4 million people in 2030. In Indonesia, cancer became the third largest

contributor to death after heart disease.[1] Nowadays, the attempt to find new anticancer drug

continue to be done because the old drugs which have long been used gradually become less

effective and there is a tendency of resistance.[2]

Classical anticancer drugs which are used in chemotherapy are a group of the alkylating

compound, antimetabolite, anthracycline antibiotics, plant alkaloids, topoisomerase

inhibitors. The drugs in those groups inhibit the cell division or the synthesis and function of

DNA in some ways. New anticancer drugs do not affect DNA directly, but they affect

specific target molecule which is needed for carcinogenesis and the growth of cancer cells,

known as molecularly targeted therapy.[3] Anticancer which are included in this group are

tyrosine kinase inhibitors (small molecule tyrosine kinase inhibitor) and monoclonal

antibodies. Tyrosine kinase inhibitors which have become the anticancer drugs are imatinib,

gefitinib, and erlotinib. Oncologists believe that the target molecule therapy is the future

chemotherapy. Hydroxy Urea (Hydrea®) also used as anticancer has distinctive features that

are able to produce nitric oxide, which is a vasodilator may cause antitumor effects. RNR

inhibition by hydroxy urea possibility for it has an unpaired electron that can extinguish

tyrosine radicals.[4]

Modern drug development is conducted through a long process and also spends great costs

because in the beginning, it was just a trial (trial and error). To minimize the nature of the

trial, the drug designing is performed (drug design), which is developing drugs which

molecular structure and biological activity have been known, based on the reasoning that is

systemic and rational by Quantitative Relationships Structure and Activity method

(QSAR).[5] In the era of a computer nowadays, the approach of structure and activity

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www.wjpr.net Vol 7, Issue 07, 2018. 58 The first step in drug designing is determining the lead compound which structure will be

modified to obtain the desired activity, to increase the activity or selectivity, or to decrease

the toxicity. After that the modification of the lead compound structure was done. Urea is one

of the lead compounds, which is the parent of many derivatives which are potential as

anticancer. Urea derivative, which is well known as an anticancer drug, is carmustine or

BCNU (bis-chloroethylnitrosourea) and ENU (N-ethyl-N-nitrosourea) which include

alkylating compounds, and hydroxyurea (HU) which works by inhibiting ribonucleotide

reductase enzyme.[7] ENU can induce mutations and also toxic at the high dose. The structure

of these compounds is shown in Figure 1.

Figure 1. Structure of Carmustine (a), ENU (b), hydroxyurea (c), and N_{-phenylurea (d)}

Siswandono has done the synthesis of a new compound which is derived from benzoylurea

that has activity on the central nervous system in the form of movement disorder in mice.[8]

Susilowati and Diyah reported that N-benzoylurea, N-4-methoxy-benzoylurea, and

2-chlorobenzoylurea showed a cytotoxic effect based on BST (Brine Shrimp Lethality Test) so

that it is potential as an anticancer.[9] The cytotoxic activity of those compounds is higher

than hydroxyurea, which has been known as an anticancer drug. Hydroxyurea and its

hydroxamic acid derivatives are reported to work by affecting the specific enzyme target

involved in the cancer development such as histone deacetylase, matrix metalloproteinase

(MMP), and RNR.[10,11] The cytotoxic activity by Brine Shrimp Test is also indicated of the

cells die, this method is also used to determine the cytotoxic activity of the 11 bases ketone

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www.wjpr.net Vol 7, Issue 07, 2018. 59 of the structure of urea by synthesizing 9 derivatives 1- (benzoyloxy) urea and concluded that

there is a linear relationship between in vitro cytotoxic activity against HeLa cell with the

activity which is predicted to be conducted in silico through molecular modeling. The activity

of cytotoxic derivative 1- (benzoyloxy) urea is higher than hydroxyurea.[14]

In the attempt to get a new anticancer compound, urea structural modifications are done. The

structure of anticancer compounds contains NH-CO-NH moiety, which is a pharmacophoric

group. In some compounds, such pharmacophore is in an aromatic heterocyclic ring or in the

nonaromatic heterocyclic ring, as -NH or -N-.[15] By maintaining pharmacophore

-NH-CO-NH- and also adding an alkyl group in form of ethyl and an aromatic group, N-phenylurea

derivative compound will be made (figure 2).

For the optimization of anticancer activity, modification of the structure of phenylurea

compound based on the change of the nature of lipophilic, steric and compound electronic is

done. The modification of lipophilic and electronic properties is done based on the Topliss

model[16], which is by including the substituent in the benzene ring, which is predicted to

produce compounds with higher activity than the parent compounds. Substituent methyl

(-CH3) will increase the non-polar lipophilic properties, while the electronegative substituents

such as methoxy, chloro, and trifluoromethyl will change the electronic properties of

compounds. The modification of steric properties results from the change in the size of

benzoyl group which has been substituted.[17] Compound and N-phenylurea derivatives can

be synthesized through the reaction between N-phenylurea compound with benzoyl chloride

based on Schotten-Baumann method.[18]

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www.wjpr.net Vol 7, Issue 07, 2018. 60 To test the anticancer activity, the determination of cell growth inhibition activity by in vitro

is done.[19] Tumor cell culture which is used is cervical carcinoma cell (HeLa cells). The

activity of cell growth inhibition is expressed in IC50, namely the concentration of drug that

can result in the death of 50% of tumor cells.[20]

From this research, a new compound which is derived from N-phenylurea which can be used

as an anticancer drug will be obtained.

RESEARCH METHOD

Materials

The materials for synthesis and physicochemical analysis have grades of pro analysis:

N-phenylurea, pyridine, various organic solvents (acetone, ethyl acetate, n-hexane, chloroform,

ethanol, and methanol), Kieselgel 60 F254, DMSO-d6. And were purchased from the Sigma

Aldrich and E.Merck.

The materials for the anticancer activation test: synthesized compound, HeLa cell culture,

Culture Media DMEM, DMSO solution, phosphate buffer saline (PBS), MTT (3-

(4,5-dimetiltiazol-2-il) -2,5-diphenyltetrazolium bromide), SDS 10% in 0.1 N HCl.

Tools

The tools used for the synthesis and structural analysis of compounds: glassware in the

laboratory, Spectrophotometer UV-vis Shimadzu HP, 8452 A, Spectrophotometer Jasco

FT-IR 5300, Spectrometers, 1H-NMR Hitachi R-1900, Electrothermal Mel-Temp, Corning Hot

Plate P 351, Analytical balance Shimadzu LM-20. The tools for cytotoxic test: Micropipette

200, 1000 μL and tip, test tube, microplate, conical tube, ELISA-Reader.

Synthesis procedure of N_{-phenylurea derivate}

In a round bottom flask of 200 ml, 0.03 mol of N-phenylurea is mixed with 40 ml of

tetrahydrofuran and 4 ml of pyridine. At a temperature of 5º C, 4-methoxybenzoyl chloride

derivative solution of 0.01 mol is added to 20 ml of tetrahydrofuran, bit by bit while stirred

with a magnetic stirrer. After the benzoyl chloride derivative solution is out, the mixture is

refluxed and stirred for 3 hours.

After the reaction is stopped, tetrahydrofuran is evaporated on a rotavapor (rotary

evaporator). On the result of the reaction, saturated sodium bicarbonate solution is added

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www.wjpr.net Vol 7, Issue 07, 2018. 61 funnel, the solids are washed with 50 ml of water 2 times, then they are washed with 10 ml of

ethanol two times.

Recrystallization is done by dissolving the solids with ethanol as necessary while it is stirred

above the heater (hot plate). The solution is filtered in hot condition, the filtrate is left at room

temperature until it is cool and then left it for a night. A crystal which is formed is filtered

through a Buchner funnel, and then it is washed with 10 ml of ethanol two times. Depending

on the synthesized compounds obtained, recrystallization can be done with another solvent

which is suitable, such as acetone-water. A crystal which is formed is moved into a petri dish,

and then it is dried in an oven.

Purity Test and Compound Structure Identification

The purity test of the synthesized compounds is done by thin layer chromatography, through

stationary phase Silica gel GF254 plate aluminum E. Merck and UV lamp stain appeared. A

melting point determination is done by electrothermal melting point apparatus. The

identification of the structure of the synthesized compound is performed by a

spectrophotometer ultra-violet (UV) and infrared (IR), nucleus magnetic resonance

spectrometry (1H-NMR) and mass spectrometry (MS).[21]

Anticancer Activity Test

Anticancer activity is determined through the cytotoxic test in vitro by using cancer cells

(HeLa cell line) with MTT method. Parent solution from 10 test compounds at the level of

5000 μg/ mL in DMSO solvent. From each of the parent solution, a series of working

standard solution with concentrations of 250, 500, 750, 1000, 2000 μg/ mL are made by

dilution. The standard solution of anticancer drug (hydroxyurea) is also made as a positive

control and solvent blank as a negative control.

The culture of cancer cell and the normal cell is prepared in the form of a cell suspension

with a density of 105-2.106. Then the cell is inserted into Microplate wells, each is 100 L,

except the well used as a control media. A total of 0.2 mL of each standard work, positive

control, and negative control, is included in the microplate. For each concentration of the

working standard solution, three replications are made. The microplate is incubated in an

incubator of 5% CO2 for 24 hours at a temperature of 37º C and pH of 7.4 to 7.7. MTT

reagent is prepared for treatment (0.5 mg/ ml) by diluting 1 mL of stock MTT (50 mg MTT

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www.wjpr.net Vol 7, Issue 07, 2018. 62 After incubation, the cell media is removed, and the cell is washed with PBS. Then, 100 μL

of MTT reagent is added to each well, including media controls (without cells). The plate is

incubated for 2-4 hours in a CO2 incubator. After formazan is formed, 100μL SDS 10% is

added in 0.1 N HCl. The plate is wrapped in aluminum foil and incubated in a dark place at

room temperature for a night. Furthermore, the absorbance of each of the wells is observed

by ELISA reader at 595 nm. The more the number of cells live, the greater the absorbance.

IC50 of the test compound is determined by probit regression analysis. The same procedure is

also performed for normal cells.

RESULT

Synthesis of N_{-4-methoxybenzoyl-}N_'-phenylurea

Synthesis result in the form of white needle-shaped crystals with a yield of 51,23 %, it shows

that the method of Schotten-Bauman was the elected method of the synthesis process to

produced the N-4-methoxybenzoyl-N'-phenylurea compound. Identification of the structure

of the active compound N-4-methoxybenzoyl-N'-phenylurea can be seen in the following

analysis.

In the next stage test, thin-layer chromatography on compounds synthesized by using 2

different solvents (n-hexane: acetone = 4:2 and n-hexane: ethyl acetate = 4:2) gave a single

spot with different Rf compound with the N-phenylurea as the parent compound. The above

shows that the desired compounds synthesized have been formed and relatively pure

compounds also have different from the parent compound. At the melting point analysis of

test compound that had been synthesized had a melting point (158oC) and had a difference

with the parent compound which had the melting point (145oC). In this test has proven that

the compound which was synthesized has been formed and has a relative purity because there

were no other impurities in it.

Characterization of the compound

N-4methoxybenzoyl-N'-phenylurea, λ max (nm)(Ethanol) = 252; IR (KBr pellet), 3446 cm-1

(NH secondary), 1685 cm-1 (-CO), 1538 cm-1 (C=C arom); 1-HNMR (DMSO-d6), 6.90–8, 10,

m, (C6H5), 12.00-13.00, d, (NH); 3.70-4.10, m, (OCH3); MS (EI), 270 (M)+, 151

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www.wjpr.net Vol 7, Issue 07, 2018. 63 Anticancer activation test of N_{-methoxybenzoyl-}N_{'-phenylurea compound and Hidroxy}

Urea

After the anticancer test is conducted in N-4-methoxybenzoyl-N'-phenylurea compound and

hydroxyl urea by using MTT assay method, the result can be seen in Figure 1 and 2 below.

Figure 1: The result of anticancer activation test of N_{-4-methoxybenzoyl-}N_'-phenylurea

compound.

IC50 = 1757 ug/mL = 6.50 mM

Figure 2: The result of anticancer activation test of hidroxy urea as a standard

compound.

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www.wjpr.net Vol 7, Issue 07, 2018. 64 DISCUSSION

The synthesis of N-4-methoxybenzoyl-N'-phenylurea is carried out by reacting the primary

amine group of N-phenylurea with 4-methoxybenzoyl chloride group of benzoyl chloride.

Several researchers have done some method of reaction between primary amine group with

the derivative of benzoyl chloride. Reksohadiprodjo,1987 and Tjiptasurasa,1991 have done

acylation reaction between the derivative of urea with an acyl chloride, the method used is by

mixing and heating at the temperature of 60 - 80oC.[22;23] It turns out that the result obtained

has a relatively small yield (9-31%), this can be due to the lack of contact between the

compounds because there are no solvent media which is used. The method above is used by

researchers because urea derivative has different solubility properties with acyl chloride

derivative. Urea derivative is a compound that is water soluble, while the derivative of acyl

chloride is not water soluble. On the implementation of acylation reaction, water is avoided

because the presence of water can react with benzoyl chloride derivatives and it will form

benzoic acid. Alcohol solvent also should not be used because it will form ester compound if

it is reacted with benzoyl chloride derivative. In the acylation reaction, benzoyl chloride

derivative can react quickly and perfectly with primary, secondary, or tertiary amine

compounds in the use of pyridine solvent and gives the percentage of result which is

relatively good.[24]

In the acylation reaction between urea compound with a derivative of acyl chloride,

theoretically both primary amine groups can react with the derivative of acyl chloride, but the

result of research that has been done by Reksohadiprodjo, 1987 and Tjiptasurasa, 1991 shows

that only one primary amine group of urea compound reacts with acyl chloride derivative.

This is due to the influence of the space obstacle of aromatic nucleus so that it will interfere

the next reaction. In the acylation reaction, HCl will be released. It can interfere the process

of the reaction because the amide group which is formed will be parsed back, therefore it can

be overcome with the addition of 2 equivalents of the amine compound. Another way to

overcome the problems above is by neutralizing HCl which is formed by adding a strong base

such as sodium hydroxide or potassium hydroxide solution. The reaction between amine

compound with acyl chloride using organic solvent is known as Schotten-Baumann

reaction.[18;24]

Bambang Soekardjo (1989) has conducted the acylation reaction between ampicillin and