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ABSTRAK
Jesanta Ansera Tarigan. 2015. “Entomopatogenik Beauveria Bassiana Vuill. dari Berbagai Media Tumbuh Terhadap Hama Ulat Grayak (Spodoptera litura F.) (Lepidoptera : Noctuidae) Pada Tanaman Tembakau Di Rumah Kasa”. Di bawah bimbingan M. Chyccu Tobing dan Syahrial Oemry.
Penelitian dilaksanakan di Laboratorium Penyakit Tumbuhan dan Rumah Kasa, Fakultas Pertanian, Universitas Sumatra Utara. Penelitian dilaksanakan pada bulan Maret-April 2015. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) factorial. Faktor 1 jenis media tumbuh Beauveria bassiana M0: Kontrol (Medium DOC PDA 02), M1: serbuk kayu rambung, M2: serbuk kayu mahoni, M3: serbuk kayu ubi kayu dan faktor 2 waktu aplikasi Beauveria bassiana S1: Beauveria bassiana di aplikasian setelah Spodoptera, S2: Beauveria bassiana di aplikasian sebelum Spodoptera. Hasil penelitian menunjukkan: Preverensi medium dengan konsentrasi spora tertinggi terdapat pada medium serbuk kayu ubi yaitu sebesar 9.913,333 cfu spora B. bassiana/ 10 g medium aplikasi. Medium serbuk kayu ubi kayu secara konstan memiliki nilai mortalitas tertinggi 4-7 Has. Medium serbuk ubi kayu memiliki nilai keparahan serangan terkecil yaitu 10,270%. Lethal time 50 (LT 50) tercepat terhadap medium terdapat pada perlakuan medium serbuk kayu ubi yaitu sebesar 4,131 hari.
Kata kunci : Beauveria Bassiana, media tumbuh, waktu aplikasi dan
Spodoptera litura.
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ABSTRACT
Jesanta Ansera Tarigan. 2015. Entomophatogenict of Beauveria Bassiana Vuill. from Various Medium to Control Oriental Lifeworm Moth (Spodoptera litura F.) (Lepidoptera : Noctuidae) on Tobacco in Screen House”. Under direction M. Chyccu Tobing and Syahrial Oemry.
The research was conducted in Plant Disease Laboratory and Screen House, Agriculture Faculty, University of Sumatra Utara from March to April 2015. It was done by using Factorial Completely Randomized Design (FCRD) with first factor, various medium culture B. bassiana, treatments: M0: control (DOC PDA 02), M1: dust of rubber plant, M2: dust of mahogany, M3: dust of cassava and second factor application time Beauveria bassiana S1: Beauveria bassiana was applicated after Spodoptera, S2: Beauveria bassiana was applicated before Spodoptera. The result showed : Preverention medium with concentration maximum spores in dust cassava media was 9.913,333 cfu spores B. bassiana/ 10 g application medium. Cassava dust media had constant maximum mortality 4-7 days after application. Cassava dust media had minimum value of severity attack was 10,270%. Lethal time 50 (LT 50) efectivities on cassava dust media was
4.131 days.
Word key: Beauveria Bassiana, culture medium, time application and Spodoptera litura.
