Isolat-isolat lokal terdiri atas 16 biotipe berdasarkan sifat biokimianya. Parameter yang digunakan dalam proses biotyping ini adalah 10 dari 19 uji biokimia pada RapID onE.
Dari 17 isolat yang diamplifikasi gen infBnya, maka ditemukan 14 isolat memiliki gen tersebut. Tiga isolat yang tidak memiliki gen infB, dimana ketiga isolat tersebut teridentifikasi sebagai C. turicensis berdasarkan 4 reaksi biokimia Iversen.
Dari 6 isolat lokal Cronobacter spp. yang dianalisa keragaman Gen infBnya, maka isolat lokal dibedakan atas dua kelompok. Isolat DES b7a, DES b10, DES c13, YR t2a dan E6 memiliki kekerabatan yang dekat dengan isolat C. sakazakii ATCC BAA-894 dan C. sakazakii ES15. Isolat YR c3a memiliki
hubungan kekerabatan yang dekat dengan isolat C. malonaticus 681 WGS dan C. dublinensis 582 WGS. Gen infB dapat digunakan untuk mengkonfirmasi hasil
identifikasi berdasarkan gen 16S rRNA yakni dengan mampu memisahkan spesies C. sakazakii dan C. malonaticus.
Saran
Perlu dilakukannya penelitian lanjutan untuk validasi parameter uji biokimia Iversen ditambah uji pyrohydonyl-β-naphthylamide dan 10 uji biokimia RapID onE dalam melakukan biotyping isolat Cronobacter spp. Sedangkan uji karakterisasi genetik berdasarkan gen infB masih perlu dikembangkan dengan menggunakan primer berbeda sehingga dapat diperoleh peran gen ini secara lengkap.
DAFTAR
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