Staff Site Universitas Negeri Yogyakarta

(1)

Cell Structure and Function

evy yulianti

(2)

Cell Types

• _Prokaryotic

(3)

Eukaryotic cells have internal

membranes that compartmentalize

their functions

• _{The basic structural and functional unit of every}

organism is one of two types of cells: prokaryotic or eukaryotic

• _{Only organisms of the domains Bacteria and Archaea}

consist of prokaryotic cells

• _{Protists, fungi, animals, and plants all consist of}

(4)

Cell Structure

•

All Cells have:

–

an outermost plasma membrane

(5)

Cell Structure

•

All Cells have:

–

an outermost plasma membrane……

•

Structure – phospholipid bilayer with

embedded proteins

•

Function – isolates cell contents, controls

(6)

Cell Structure

•

All Cells have:

–

genetic material in the form of DNA

•

Eukaryotes – DNA is within a membrane

(nucleus)

•

Prokaryotes – no membrane around the

(7)

Cell Structure

•

All Cells have:

–

cytoplasm with ribosomes……

•

Cytoplasm – fluid area inside outer

plasma membrane and outside DNA

region

(8)

Why Are Cells So Small?

•

Cells need sufficient surface area to allow

adequate transport of nutrients in and wastes

out.

•

As cell volume increases, so does the need for

(9)

Why Are Cells So Small?

•

However, as cell volume increases the surface

area of the cell does not expand as quickly.

– _{If the cell gets too large a volume it cannot}

transport enough wastes out or nutrients in.

(10)

Comparing Prokaryotic and

Eukaryotic Cells

• _{Basic features of all cells:} – _{Plasma membrane}

– _{Semifluid substance called the cytosol} – _{Chromosomes (carry genes)}

(11)

• _{Prokaryotic cells have no nucleus}

• _{In a prokaryotic cell, DNA is in an unbound region}

called the nucleoid

(12)

A typical rod-shaped bacterium

A thin section through the bacterium Bacillus

coagulans (TEM)

(13)

• Eukaryotic cells have DNA in a nucleus that is bounded by a membranous nuclear envelope

• _{Eukaryotic cells have membrane-bound organelles} • _{Eukaryotic cells are generally much larger than}

prokaryotic cells

• _{The logistics of carrying out cellular metabolism sets}

(14)

Prokaryotic Cell Structure

•

Prokaryotic Cells are smaller and simpler

in structure than eukaryotic cells.

•

Typical prokaryotic cell is ________

•

Prokaryotic cells do NOT have:

–

Nucleus

(15)

Prokaryotic Cells

•

No membrane bound nucleus

•

Nucleoid = region of DNA concentration

(16)

(17)

Prokaryotic Cell Structure

•

Structures

– _{Plasma membrane} – _{Cell wall}

– _{Cytoplasm with ribosomes} – _Nucleoid

– _Capsule

(18)

Eukaryotic Cells

•

Structures in all eukaryotic cells

– _Nucleus

– _Ribosomes

– _{Cytomembrane System}

• _{Endoplasmic reticulum} • _{Golgi body}

• _Vesicles

(19)

Eukaryotic Cells

•

Nucleus bound by membrane

•

Include fungi, protists, plant,

and animal cells

•

Possess many organelles

(20)

(21)

(22)

Cytoplasm

• _{Viscous fluid containing organelles} • _{components of cytoplasm}

– _{Interconnected filaments & fibers} – _{Fluid = cytosol}

– _{Organelles (not nucleus)} – _{storage substances}

(23)

Organelles

•

Cellular machinery

•

Two general kinds

(24)

Bacteria-Like Organelles

•

Derived from symbiotic bacteria

•

Ancient association

•

Endosymbiotic theory

– _{Evolution of modern cells from cells}

(25)

Membranous Organelles

•

Functional components within cytoplasm

(26)

Nucleus

•

Function

– isolates DNA, controls movement

of substances in/out of nucleus

•

Structure

–

Nuclear envelope

•

Two Phospholipid bilayers with protein

lined pores ……

(27)

Nucleus

•

Structure,

continued

–

Nucleolus

• _{Area of condensed DNA}

(28)

The eukaryotic cell’s genetic instructions are housed in the nucleus and carried out by the ribosomes

• _{The nucleus contains most of the DNA in a eukaryotic}

cell

• _{Ribosomes use the information from the DNA to make}

(29)

The Nucleus: Genetic Library of the

Cell

• _{The nucleus contains most of the cell’s genes and is}

usually the most conspicuous organelle

• _{The nuclear envelope encloses the nucleus, separating}

(30)

Close-up of nuclear envelope Nucleus Nucleolus Chromatin Nuclear envelope: Inner membrane Outer membrane Nuclear pore Pore complex Ribosome

Pore complexes (TEM) Nuclear lamina (TEM)

1 µm Rough ER

Nucleus 1 µm

0.25 µm

(31)

Nucleus

• _{Control center of cell}

• _{Double membrane}

• _Contains

(32)

Nuclear Envelope

•

Separates nucleus from rest of cell

•

Double membrane

(33)

DNA

•

Hereditary material

•

Chromosomes

– _DNA

– _Protiens

– _{Form for cell division}

(34)

Nucleolus

•

Most cells have 2 or more

•

Directs synthesis of RNA

(35)

Nucleus

•

DNA arranged in chromosomes

–

Chromosome – single

molecule

of DNA and

its associated proteins

–

Chromatin – all of the cell’s DNA and the

(36)

Ribosomes: Protein Factories in the

Cell

• _{Ribosomes are particles made of ribosomal RNA and}

protein

• _{Ribosomes carry out protein synthesis in two locations:} – _{In the cytosol (free ribosomes)}

– _{On the outside of the endoplasmic reticulum (ER) or}

(37)

Ribosomes 0.5 µm ER Cytosol Endoplasmic reticulum (ER) Free ribosomes Bound ribosomes Large subunit Small subunit Diagram of a ribosome TEM showing ER

(38)

The Endomembrane System:

A

Review

•

The endomembrane system is a complex and

(39)

Nuclear envelope

Nucleus

Rough ER

(40)

Nuclear envelope

Nucleus

Rough ER

Smooth ER

Transport vesicle cis Golgi

(41)

Nuclear envelope

Nucleus

Rough ER

Smooth ER

Transport vesicle cis Golgi

trans Golgi

(42)

The endomembrane system regulates protein traffic and performs metabolic functions in the cell

• _{Components of the endomembrane system:}

– _{Nuclear envelope}

– _{Endoplasmic reticulum} – _{Golgi apparatus}

– _Lysosomes – _Vacuoles

– _{Plasma membrane}

• _{These components are either continuous or connected}

(43)

The Endoplasmic Reticulum:

Biosynthetic Factory

• _{The endoplasmic reticulum (ER) accounts for more}

than half of the total membrane in many eukaryotic cells

• _{The ER membrane is continuous with the nuclear}

envelope

• _{There are two distinct regions of ER:} – _{Smooth ER, which lacks ribosomes}

(44)

Ribosomes Smooth ER Rough ER ER lumen Cisternae Transport vesicle

Smooth ER Rough ER

Transitional ER 200 nm Nuclear

(45)

Endo/Cytomembrane System

•

Series of organelles responsible for:

–

Modifying protein chains into their final

form

–

Synthesis of lipids

–

Packaging fully processed proteins and

(46)

Endoplasmic Reticulum

•

Helps move substances within cells

•

Network of interconnected membranes

•

Two types

(47)

Rough Endoplasmic Reticulum

•

Ribosomes attached to surface

– _{Manufacture protiens}

– _{Not all ribosomes attached to rough ER}

(48)

Endo/Cytomembrane System

•

Endoplasmic Reticulum (ER)

– _{Rough ER (RER)}

• _{Flattened membrane sacs create a “maze”….} • _{Many ribosomes attached to the outside}

• _{Where proteins are modified and packaged for}

(49)

Functions of Rough ER

•

The rough ER

– _{Has bound ribosomes}

– _{Produces proteins and membranes, which are}

distributed by transport vesicles

(50)

Smooth Endoplasmic Reticulum

•

No attached ribosomes

•

Has enzymes that help build molecules

(51)

•

Smooth ER (SER)

– _{Tubular membrane structure} – _{Often continuous with RER} – _{No ribosomes attached}

– _{Where lipids are made and packaged for transport}

(52)

Functions of Smooth ER

•

The smooth ER

– _{Synthesizes lipids}

– _{Metabolizes carbohydrates} – _{Stores calcium}

(53)

•

Golgi Body

– _{Stack of flattened membrane sacs}

– _{Completes the processing of proteins and lipids} – _{Sorts and packages fully processed proteins and}

(54)

Golgi Apparatus

•

Involved in synthesis of plant cell wall

(55)

Golgi Apparatus Function

1. Molecules come in vesicles

2. Vesicles fuse with Golgi membrane

(56)

Golgi Apparatus Function (Continued)

4. Molecules pinched-off in separate vesicle

5. Vesicle leaves Golgi apparatus

(57)

• _{The Golgi apparatus consists of flattened membranous}

sacs called cisternae

• _{Functions of the Golgi apparatus:} – _{Modifies products of the ER}

– _{Manufactures certain macromolecules}

– _{Sorts and packages materials into transport vesicles}

(58)

trans face

(“shipping” side of

Golgi apparatus) TEM of Golgi apparatus 0.1 µm Golgi

apparatus

cis face

(“receiving” side of Golgi apparatus)

Vesicles coalesce to form new cis Golgi cisternae Vesicles also

transport certain proteins back to ER

Vesicles move from ER to Golgi

Vesicles transport specific proteins backward to newer Golgi cisternae

Cisternal maturation: Golgi cisternae move in a cis -to-trans

direction

Vesicles form and leave Golgi, carrying specific proteins to other locations or to the plasma mem-brane for secretion

(59)

(60)

Endo/Cytomembrane System

•

Transport Vesicles

– _{Vesicle = small membrane bound sac}

– _{Transport modified proteins and lipids from the ER}

(61)

•

Putting it all together

–

DNA



RNA



nuclear pore



ribosome



Protein made



protein with proper code

enters RER



proteins modified in RER and

lipids made in SER



proteins and lipids bud

(62)

Cytomembrane System

•



ER vesicles merge with Golgi body



proteins and lipids enter Golgi



each is fully

modified as it passes through layers of Golgi



modified products bud off in Golgi

(63)

Cytomembrane System

•



Golgi vesicles either merge with the plasma

(64)

Vesicles

•

Vesicles

– _{Small membrane bound sacs} – _Examples

• _{Golgi and ER transport vesicles} • _Lysosome

(65)

Lysosomes

•

Contain digestive enzymes

•

Functions

– _{Aid in cell renewal}

(66)

Lysosomes: Digestive

Compartments

• _{A lysosome is a membranous sac of hydrolytic enzymes} • _{Lysosomal enzymes can hydrolyze proteins, fats,}

polysaccharides, and nucleic acids

• _{Lysosomes also use enzymes to recycle organelles and}

(67)

(68)

Autophagy: lysosome breaking down damaged organelle 1 µm Vesicle containing damaged mitochondrion Mitochondrion fragment Lysosome containing two damaged organelles

Digestion

Lysosome

(69)

Vacuoles

•

Membrane bound storage sacs

•

More common in plants than animals

•

Contents

(70)

Vacuoles: Diverse Maintenance

Compartments

• _{Vesicles and vacuoles (larger versions of vacuoles) are}

membrane-bound sacs with varied functions

• _{A plant cell or fungal cell may have one or several}

(71)

• _{Food vacuoles are formed by phagocytosis}

• _{Contractile vacuoles, found in many freshwater}

protists, pump excess water out of cells

• _{Central vacuoles, found in many mature plant cells,}

(72)

5 µm Central vacuole

Cytosol

Tonoplast

Central vacuole Nucleus

Cell wall

(73)

Central Vacuole

•

Function – storage area for water, sugars, ions,

(74)

Central Vacuole

•

Structure

– _{Large membrane bound sac}

– _{Occupies the majority of the volume of the plant}

cell

– _{Increases cell’s surface area for transport of}

(75)

Plant Cell Structures

•

Structures found in plant, but not animal cells

– _Chloroplasts – _Plastids

(76)

Bacteria-Like Organelles

•

Release & store energy

•

Types

– _Mitochondria

(release energy)

– _Chloroplasts

(77)

Origin of Mitochondria and Chloroplasts

•

Both organelles are believed to have once

(78)

Proposed Origin of Mitochondria and

Chloroplasts

•

Evidence:

– _{Each have their own DNA}

– _{Their ribosomes resemble bacterial ribosomes} – _{Each can divide on its own}

(79)

Mitochondria and chloroplasts

change energy from one form to

another

• Mitochondria are the sites of cellular respiration

• _{Chloroplasts, found only in plants and algae, are the}

sites of photosynthesis

• _{Mitochondria and chloroplasts are not part of the}

endomembrane system

(80)

Mitochondria: Chemical Energy

Conversion

• _{Mitochondria are in nearly all eukaryotic cells}

• _{They have a smooth outer membrane and an inner}

membrane folded into cristae

• _{The inner membrane creates two compartments:}

intermembrane space and mitochondrial matrix

• _{Some metabolic steps of cellular respiration are}

catalyzed in the mitochondrial matrix

• _{Cristae present a large surface area for enzymes that}

(81)

Mitochondria

•

Function – synthesis of ATP

•

Structure:

– _{~1-5 microns}

– _{Outer membrane}

– _{Inner membrane - Highly folded}

• _{Folds called cristae}

– _{Outer and inner compartment (or matrix)}

(82)

(83)

(84)

Mitochondria

(85)

(86)

Mitochondria

•

Have their own DNA

(87)

Mitochondria

•

Break down fuel molecules (

cellular respiration)

– _Glucose – _{Fatty acids}

•

Release energy

(88)

Chloroplasts

•

Derived form photosynthetic bacteria

(89)

Chloroplasts: Capture of Light

Energy

• _{The chloroplast is a member of a family of organelles}

called plastids

• _{Chloroplasts contain the green pigment chlorophyll, as}

well as enzymes and other molecules that function in photosynthesis

• _{Chloroplasts are found in leaves and other green}

organs of plants and in algae

(90)

Chloroplast

Chloroplast DNA

Ribosomes

Stroma

Inner and outer membranes

Granum

(91)

Photosynthesis

•

Takes place in the chloroplast

(92)

Chloroplasts

•

Function – site of photosynthesis

•

Structure

– _{2 outer membranes}

– _{Thylakoid membrane system}

• _{Stacked membrane sacs called granum}

– _{Chlorophyll in granum} – _Stroma

(93)

(94)

Plastids

•

Plastids are similar to vesicles

•

Examples

– _Chloroplasts

– _{Chromoplasts – contain colored pigments}

• _{Pigments called carotenoids}

(95)

Peroxisomes: Oxidation

• _{Peroxisomes are specialized metabolic compartments}

bounded by a single membrane

• _{Peroxisomes produce hydrogen peroxide and convert it}

(96)

Chloroplast Peroxisome

Mitochondrion

(97)

The cytoskeleton is a network of fibers that organizes structures and activities in the cell

• _{The cytoskeleton is a network of fibers extending}

throughout the cytoplasm

• _{It organizes the cell’s structures and activities,}

anchoring many organelles

• _{It is composed of three types of molecular structures:} – _Microtubules

– _{Microfilaments}

(98)

Cytoskeleton

•

Function

– _{gives cells internal organization, shape, and ability}

to move

•

Structure

– _{Interconnected system of microtubules,}

microfilaments, and intermediate filaments (animal only)

(99)

Microtubule

(100)

Roles of the Cytoskeleton: Support, Motility, and Regulation

• _{The cytoskeleton helps to support the cell and maintain}

its shape

• _{It interacts with motor proteins to produce motility} • _{Inside the cell, vesicles can travel along “monorails”}

provided by the cytoskeleton

• _{Recent evidence suggests that the cytoskeleton may}

(101)

Cytoskeleton

• _{Filaments & fibers}

• _{Made of 3 fiber types}

– _{Microfilaments} – _Microtubules

– _{Intermediate filaments}

• _{3 functions:}

– _{mechanical support} – _{anchor organelles}

(102)

(103)

Vesicle

Receptor for motor protein

Microtubule

of cytoskeleton Motor protein

(104)

(105)

Components of the Cytoskeleton

• _{Microtubules are the thickest of the three components}

of the cytoskeleton

• _{Microfilaments, also called actin filaments, are the}

thinnest components

• _{Intermediate filaments are fibers with diameters in a}

(106)

(107)

(108)

(109)

Microtubules

• _{Microtubules are hollow rods about 25 nm in diameter}

and about 200 nm to 25 microns long

• _{Functions of microtubules:} – _{Shaping the cell}

– _{Guiding movement of organelles}

(110)

Centrosomes and Centrioles

• _{In many cells, microtubules grow out from a}

centrosome near the nucleus

• _{The centrosome is a “microtubule-organizing center”} • _{In animal cells, the centrosome has a pair of centrioles,}

(111)

0.25 µm

Microtubule Centrosome

Centrioles

Longitudinal section

(112)

Cilia & Flagella

• _{Provide motility} • _Cilia

– _Short

– _{Used to move substances}

outside human cells

• _Flagella

– _{Whip-like extensions} – _{Found on sperm cells}

(113)

Cilia & Flagella Structure

•

Bundles of microtubules

(114)

Cilia and Flagella

• _{Microtubules control the beating of cilia and flagella,}

locomotor appendages of some cells

(115)

5 µm Direction of swimming

(116)

15 µm Direction of organism’s movement

Motion of cilia Direction of active stroke

(117)

• _{Cilia and flagella share a common ultrastructure:} – _{A core of microtubules sheathed by the plasma}

membrane

– _{A basal body that anchors the cilium or flagellum} – _{A motor protein called dynein, which drives the}

(118)

0.5 µm Microtubules Plasma membrane Basal body Plasma membrane 0.1 µm

(119)

• _{How dynein “walking” moves flagella and cilia:}

– _{Dynein arms alternately grab, move, and release the}

outer microtubules

– _{Protein cross-links limit sliding}

– _{Forces exerted by dynein arms cause doublets to}

(120)

Dynein “walking”

Microtubule

doublets

ATP

(121)

Wavelike motion Cross-linking

proteins inside

outer doublets ATP

Anchorage in cell

(122)

Centrioles

•

Pairs of microtubular structures

(123)

Microfilaments (Actin Filaments)

• _{Microfilaments are solid rods about 7 nm in diameter,}

built as a twisted double chain of actin subunits

• _{The structural role of microfilaments is to bear tension,}

resisting pulling forces within the cell

• _{They form a 3D network just inside the plasma}

membrane to help support the cell’s shape

• _{Bundles of microfilaments make up the core of}

(124)

Microfilaments (actin filaments)

Microvillus

Plasma membrane

Intermediate filaments

(125)

• _{Microfilaments that function in cellular motility contain}

the protein myosin in addition to actin

• _{In muscle cells, thousands of actin filaments are}

arranged parallel to one another

• _{Thicker filaments composed of myosin interdigitate}

(126)

Muscle cell

Actin filament

Myosin filament Myosin arm

(127)

• Localized contraction brought about by actin and myosin also drives amoeboid movement

• Pseudopodia (cellular extensions) extend and contract through the reversible assembly and contraction of

(128)

Cortex (outer cytoplasm): gel with actin network

Amoeboid movement Inner cytoplasm: sol with actin subunits Extending

(129)

• _{Cytoplasmic streaming is a circular flow of cytoplasm}

within cells

• _{This streaming speeds distribution of materials within}

the cell

• _{In plant cells, actin-myosin interactions and sol-gel}

(130)

Nonmoving

cytoplasm (gel)

Cytoplasmic streaming in plant cells

(131)

Intermediate Filaments

• _{Intermediate filaments range in diameter from 8–12}

nanometers, larger than microfilaments but smaller than microtubules

• _{They support cell shape and fix organelles in place} • _{Intermediate filaments are more permanent}

(132)

Plasma Membrane

•

Contains cell contents

(133)

Phospholipids

• _Polar

– _{Hydrophylic head} – _{Hydrophobic tail}

(134)

Movement Across the Plasma Membrane

•

A few molecules move freely

– _{Water, Carbon dioxide, Ammonia, Oxygen}

•

Carrier proteins transport some molecules

– _{Proteins embedded in lipid bilayer}

– _{Fluid mosaic model – describes fluid nature of a}

(135)

(136)

Membrane Proteins

1. Channels or transporters

– _{Move molecules in one direction}

2. Receptors

(137)

Membrane Proteins

3. Glycoproteins

– _{Identify cell type}

4. Enzymes

(138)

Molecule Movement & Cells

•

Passive Transport

•

Active Transport

•

Endocytosis

(

phagocytosis & pinocytosis)

(139)

Passive Transport

•

No energy required

•

Move due to gradient

– _{differences in concentration, pressure, charge}

•

Move to equalize gradient

(140)

Types of Passive Transport

1. Diffusion

2. Osmosis

(141)

Diffusion

(142)

Osmosis

•

Special form of diffusion

•

Fluid flows from lower solute concentration

•

Often involves movement of water

(143)

Solution Differences & Cells

•

solvent + solute = solution

•

Hypotonic

– _{Solutes in cell more than outside} – _{Outside solvent will flow into cell}

•

Isotonic

– _{Solutes equal inside & out of cell}

•

Hypertonic

(144)

(145)

Facilitated Diffusion

•

Differentially permeable membrane

•

Channels (are specific) help molecule or

ions enter or leave the cell

•

Channels usually are transport proteins

(aquaporins facilitate the movement of

water)

(146)

Process of Facilitated Transport

•

Protein binds with molecule

•

Shape of protein changes

(147)

Active Transport

•

Molecular movement

•

Requires energy (against gradient)

(148)

Endocytosis

•

Movement of large material

– _Particles – _Organisms

– _{Large molecules}

•

Movement is into cells

•

Types of endocytosis

– _{bulk-phase (nonspecific)}

(149)

Process of Endocytosis

•

Plasma membrane surrounds material

•

Edges of membrane meet

(150)

Forms of Endocytosis

•

Phagocytosis – cell eating

(151)

Exocytosis

•

Reverse of endocytosis

(152)

Exocytosis

•

Vesicle moves to cell surface

•

Membrane of vesicle fuses

(153)

Cell Walls

•

Found in plants, fungi, & many protists

(154)

Cell Wall Differences

•

Plants – mostly cellulose

(155)

Cell Wall

•

Function – provides structure and protection

– _{Never found in animal cells}

– _{Present in plant, bacterial, fungus, and protists}

(156)

Cell Wall

•

Structure

– _{Made of cellulose and other polysaccharides}

• _{May be reinforced by pectin and lignin}

(157)

Extracellular components and connections between cells help coordinate cellular activities

• _{Most cells synthesize and secrete materials that are}

external to the plasma membrane

• _{These extracellular structures include:} – _{Cell walls of plants}

(158)

Cell Walls of Plants

• _{The cell wall is an extracellular structure that}

distinguishes plant cells from animal cells

• _{The cell wall protects the plant cell, maintains its}

shape, and prevents excessive uptake of water

• _{Plant cell walls are made of cellulose fibers embedded}

(159)

Cell Walls of Plants

• _{Plant cell walls may have multiple layers:}

– _{Primary cell wall: relatively thin and flexible}

– _{Middle lamella: thin layer between primary walls of}

adjacent cells

– _{Secondary cell wall (in some cells): added between}

the plasma membrane and the primary cell wall

(160)

Central vacuole of cell Plasma membrane Secondary cell wall Primary cell wall Middle lamella 1 µm Central vacuole of cell Central vacuole Cytosol Plasma membrane

Plant cell walls

(161)

(162)

(163)

The Extracellular Matrix (ECM) of

Animal Cells

• _{Animal cells lack cell walls but are covered by an}

elaborate extracellular matrix (ECM)

• _{The ECM is made up of glycoproteins and other}

macromolecules

• _{Functions of the ECM:} – _Support

(164)

(165)

(166)

Intercellular Junctions

• _{Neighboring cells in tissues, organs, or organ systems}

often adhere, interact, and communicate through direct physical contact

(167)

Plants: Plasmodesmata

• _{Plasmodesmata are channels that perforate plant cell}

walls

• _{Through plasmodesmata, water and small solutes (and}

(168)

Interior of cell

Interior of cell

0.5 µm Plasmodesmata Plasma membranes

(169)

Animals: Tight Junctions, Desmosomes, and Gap Junctions

• _{At tight junctions, membranes of neighboring cells are}

pressed together, preventing leakage of extracellular fluid

• _{Desmosomes (anchoring junctions) fasten cells together}

into strong sheets

• _{Gap junctions (communicating junctions) provide}

(170)

Tight junctions prevent fluid from moving across a layer of cells

Tight junction 0.5 µm 1 µm 0.1 µm Gap junction Extracellular matrix Space between cells Plasma membranes of adjacent cells

(171)

The Cell: A Living Unit Greater Than the Sum of Its Parts

• _{Cells rely on the integration of structures and}

organelles in order to function

• _{For example, a macrophage’s ability to destroy bacteria}

(172)

(173)

(174)