LAMPIRAN
Lampiran 1. Gambar Penelitian
Lampiran 2 . Komposisi Media MS (Murashige & Skoog) 1962
Bahan Kimia Makro Nutrien
NH4NO3
Mikro Nutrien MnSO4 . 4H2O
Vitamin Glycine Nicotine Acid Pyrodoxin HCl Thyamine HCl Myo-inositol pH
Gula Agar
Konsentrasi Dalam Media (mg/l)
1650,000 1900,000 440,000 370,000 170,000
37,000 100,000
5,8 3g 7g
Sum of Squares
df Mean
Square
F Sig.
Between Groups
Within Groups Total
Deviation
Std.
Levene Statistic
df1 df2 Sig.
4,960 3 20 ,010
33
Lampiran 3. Data Berat Kering Kalus (g) dan Uji Statistik
Perlakuan U1 U2 U3 U4 U5 U6 Jumlah Rata-rata
Uji Statistik
Descriptives Ulangan
Test of Homogeneity of Variances Ulangan
Perlakuan_bb s
N Subset for alpha = 0.05
1 2 3
K1 K3 K2 K0 Sig.
6 6 6 6
,1833 ,2067
,237
,2067 ,2400 ,097
,2400 ,2617 ,271 Ulangan
Duncana
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
Sum of Squares
df Mean
Square
F Sig.
Between Groups
Within Groups Total
Deviation
Std.
Levene Statistic
df1 df2 Sig.
,833 3 20 ,491
35
Lampiran 4. Indeks Pertumbuhan (g) dan Uji Statistik
Perlakuan U1 U2 U3 U4 U5 U6 Jumlah Rata-rata Uji Statistik
Descriptives Ulangan
Test of Homogeneity of Variances Ulangan
Perlakuan_bb s
N Subset for alpha = 0.05
1 2 3
K1 K3 K2 K0 Sig.
6 6 6 6
,1100 ,1300
,125
,1300 ,1483 ,158
,1483 ,1683 ,125 Ulangan
Duncana
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
37
Lampiran 5. Luas Area Standar, Retention Time Linear
dan Uji Statistik Regresi
Konsentrasi ( ppm )
250 500 750 1000
Rata-rata
Uji Statistik
SUMMARY OUTPUT
Regression Statistics
Rata-Rata Luas Area
1210266,5 2142538,5 3643158 6249108,5
Rata-Rata Retention Time
Multiple R R Square Adjusted R Square
Standard Error Observations
0,97506 0,950742 0,926114 598077,7 4
ANOVA
Significance
df SS MS F F
Regression Residual Total
1 2 3
1,38E+13 1,38E+13 38,60289 7,15E+11 3,58E+11
1,45E+13
0,02494
Standard
L
uas A
re
a
Lampiran 6. Kurva Analisis Regresi Linear Standar Katekin
7000000
6000000 y = 8215,14x - 2146753 R²= 0,97729 5000000
4000000
3000000
2000000
1000000
0
0 200 400 600 800 1000 1200
Konsentrasi ( ppm )
39
Lampiran 7. Luas Area Perlakuan dan Retention Time
Perlakuan
K0 K1 K2 K3
Rata-Rata Luas Area
536592 1168564 5913723 874057
Rata-Rata Retention Time
Lampiran 8. Retention Time Standar dan Sampel
Standar (ppm) Retention time
Ulangan 1 Ulangan 2
Rata- Rata
250 500 750 1000 Rata-Rata
Sampel
K0 K1 K2 K3 Rata-Rata
3,3010 3,3090 3,3080 3,3300
Retention time Ulangan 1
3,3840 3,3890 3,5410 3,3920
3,3040 3,3160 3,3180 3,3200
Ulangan 2 3,3970 3,3820 3,5400 3,3920
3,3025 3,3125 3,3130 3,2800 3,3020
Rata- Rata
3,3905 3,3855 3,5405 3,3920 3,4271
41
Lampiran 9. Analisis Kandungan Katekin Secara Kuantitatif dan Uji Statistik
Perlakuan
K0 K1 K2 K3
Rata-Rata Luas Area Sampel
536592 1168564 5913723 874057
Kadar Katekin (ppm)
Statistic
N Mean
Std. Deviation Std. Error Kontrol
Lower Bound 95% Confidence Interval for Mean
Upper Bound Minimum
Maximum N
Mean
Std. Deviation Std. Error UV C 15
Lower Bound 95% Confidence Interval for Mean
Upper Bound Minimum
Maximum N
Mean
Std. Deviation Std. Error UV C 30
Lower Bound 95% Confidence Interval for Mean
Upper Bound Minimum
Maximum N
Mean
Std. Deviation Std. Error UV C 45
Lower Bound 95% Confidence Interval for Mean
Upper Bound Minimum
Maximum N
Mean
Std. Deviation Std. Error Total
Lower Bound 95% Confidence Interval for Mean
Upper Bound Minimum
Maximum
2 619697,0000 117528,21810 83105,00000 -436252,1446 1675646,1446 536592,00 702802,00 2 1137573,5000 43827,18540 30990,50000 743801,8621 1531345,1379 1,11E+006 1,17E+006 2 5817846,5000 135589,84661 95876,50000 4599620,0616 7036072,9384 5,72E+006 5,91E+006 2 957563,0000 118095,31774 83506,00000 -103481,3327 2018607,3327 874057,00 1,04E+006 8 2133170,0000 2284402,89467 807658,38889 223361,3866 4042978,6134 536592,00 5,91E+006
Uji Statistik
Descriptives Ulangan
Sum of Squares
df Mean Square F Sig.
Between Groups
Within Groups
Total
36481411281 285,000 48064814807,
000 36529476096
092,000
3
4
7
121604704270 95,000 12016203701,
750
1012,006 ,000
Perlakuan N Subset for alpha = 0.05
1 2 3
Kontrol UV C 45
619697,0000
1,000
957563,0000 1137573,5000
,176
5817846,5000
1,000 Levene
Statistic
df1 df2 Sig.
116511859656 4764,000
3 4 ,000
43
Test of Homogeneity of Variances Ulangan
ANOVA Ulangan
Ulangan Duncana
Lampiran 10. Out Put High Performance Liquid Chromatography ( HPLC )
a. Out put Standar Katekin 250 Ppm
b. Out put Ektrak Katekin Disinari UV C 30 menit
45
Lampiran 11. Perhitungan Kandungan Katekin Sampel Secara Kuantitatif
Y = bx + a
Y = 8215,14 x - 2146753 Keterangan:
Y = Luas Area Sampel b = koefisien regresi
a = konstansta luas area standar x = kadar katekin
Kandungan katekin kontrol Y = bx + a
536592 = 8215,14 x – 2146753 -8215,14 x = -536592 – 2146753
