• Tidak ada hasil yang ditemukan

information of genomics quantitative lecture outline

N/A
N/A
Protected

Academic year: 2017

Membagikan "information of genomics quantitative lecture outline"

Copied!
1
0
0

Teks penuh

(1)

Lecture One: Principles of Genetic Linkage and Map Creation  Background – Thomas Hunt and Alfred Sturtevant with the

idea that mutations can be in linkage. Recombination between traits - crossing over during meiosis leading to disruption between traits. The use of number of recombinations to separate traits, distance in centimorgans.

 Genetic markers used for analysis, types of markers available. Crossing between two populations. The idea of a genetic map.  Methods for measuring genetic distances. Use of three (and

more) markers to establish gene order and distance (travelling salesman problem). Mapping functions (Kosambi, Haldane). Practical One: Genetic Map construction.

 Use R (maybe crimap?) to perform a map construction on either a simulated or real (but limited) dataset.

 Try different methods of map construction. Counting the number of recombinations.

Lecture Two: Principles of QTL analysis

 Introduction to QTL analysis, definition of a QTL. Differences between Mendelian (simple) and quantitative (complex) traits.  Crossing populations – types of QTL crosses (backcross, F2

intercross, advanced intercross, recombinant inbred lines).  Measuring phenotypes and the regression of phenotypes on

marker genotypes. Placing traits on the genetic map.  Statistical methods for single marker analysis (T test!).

Problems with this approach (close and distant linkage confounded with large and small effect size).

 Interval mapping and its advantages.

 Composite Interval Mapping, Multiple Interval Mapping and beyond. Introduction to these only!

 Introduction to eQTL analysis and gene networks.  Advantages and disadvantages of QTL analysis.

Practical Two: QTL analysis in R

 Use of R/QTL to test mapping using an experimental dataset. Types of analysis.

 Preparation of a dataset. Plotting phenotypes, checking for outliers, data cleaning.

 Segregation distortion (possibly practical one?).  Single marker analysis using regression.

 Interval mapping analysis using Haley-Knott regression and ML.

Referensi

Dokumen terkait

Berdasarkan Penetapan Hasil Kualifikasi Nomor : 602.06.01.d/PPBJ.KONS - PU.BM/2013 tanggal 17 Mei 2013 atas Pengadaan Jasa Konsultansi Pengawasan Teknis DAK + DAU (Kegiatan

diskusi kelompok kecil, keterampilan mengajar perorangan dan keterampilan mengelola kelas. Keterampilan dasar mengajar guru tersebut merupakan satu

1. Sejarah Pendirian Masjid Al-Bantani ... Error! Bookmark not defined. Letak Masjid Al-Bantani ... Error! Bookmark not defined. Konstruksi Bangunan Masjid Al-Bantani Error!

In many varieties of Syrian Arabic, the suffix –tun (pronounced / t(a/e)n/ in the vernacular) is used for both genders, a common process known as simplification or

Sehubungan dengan telah dilakukan evaluasi dokumen kualifikasi maka sesuai dengan jadwal pada SPSE, dengan ini kami mengundang saudara untuk mengikuti pembuktian kualifikasi

Soekamo, Ende mengumumkan Rencana Umum Pengadaan Barangl Jasa untuk pelaksanaan kegiatan Tahun Anggaran 2012, seperti tersebut dibawah

Seperti pada Pondok Pesantren Modern Al- Mu’awanah yang tentunya belum menggunakan sistem informasi pada proses kerjanya, Khususnya pada proses kerja keuangan sekolah

Pada Hari ini, Jumat Tanggal Empat Belas Bulan Agustus Tahun Dua Ribu Lima Belas , Panitia Pengadaan Barang/Jasa Satker Loka Pemeriksaan Penyakit Ikan dan