PROCEEDING IBC V Hadiyanto Combine

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The 5

th

Indonesia

Biotechnology

Conference

An Interna tiona l Forum

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The 5

th

Indonesia

Biotechnology

Conference

An Internationa l Forum

P R O C E E D I N G S

Ma ta ra m , July 4th-7th 2012

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The 5

th

Indonesia

Biotechnology

Conference

An Internationa l Forum

P R O C E E D I N G S

July 4

th

_-7

th

₂₀₁₂

Sa ntosa Ho tel, Seng g ig i Bea c h

M a ta ra m , Lo m b ok Isla nd

Provinc e of We st Nusa Teng g a ra , Ind o nesia

Hosted by:

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7 Invited Spea kers (Plenary Session)

... 27

Lunch Symposia ... 57

Concurrent Session & Post er Present at ions ... 60

Agricult ure & Forest ry Biot echnology ... 61

Energy & Environm ent al Biot echnology ... 463

Healt h & M edical Biot echnology ... 702

Indust rial Biot echnology ... 919

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LIST OF PARTICIPANT ... 1009

LIST OF THE 5

th

_{IBC COMMITTEE... 1047}

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9

The 5th_{Ind o nesia Bio tec hno lo g y C onferenc e}

M a ta ra m , July 4-7th₂₀₁₂

Foreword from Cha irma n of Orga nizing

Committee

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11 Foreword from Rector of Universita s

Indonesia

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13 Genera l Sche dule : Da y 1

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17 Genera l Sche dule : Da y 2

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M a ta ra m , July 4-7th₂₀₁₂ << Back t o t able of cont ent s

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The Utilization of Palm Oil M ill Effluent (POM E) for

Bioenergy through W ild Algae Treatment

Hadiyant o

Cent er of Biom ass and Renew able Energy (CBIORE), Depart m ent of Chem ical Engineering, Facult y of Engineering, Diponegoro Universit y,

Jl. Prof. Sudhart o, SH-Tem balang Sem arang 50239 hady.hadiyant o@gmail.com filt ered and m easured as dry w eight . Result indicat ed t hat Chlorella sp at 50% POM E 1gr/ L urea show ed higher specif ic grow t h rat e (0.066/ day). Fact or affect ing grow t h rat e of m icroalgae is CNP rat io, POM E concent rat ion, and urea concent rat ion

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1. Introduction

Table 1. Com m odit ies of Indonesia agricult ure 2010-2014

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can not be avoided because wit hin 15 years, t he oil reserve w ill be dim inished; t he exploring of new renew able energy resources is a vit al. M icroalgae can be considered as t he one w hile POM E w hich has high COD is also pot ent ial as biogas sources. To be used as nut rient of m icroalgae, POM E m ust have low BOD cont ent s.

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2.2. Algae cult ure

Wild algae was obt ained from pond area in PTPN VII Lam pung and Bioprocess Laborat ory, Diponegoro Universit y.The w ild algae

w ere ident ified for t heir species at Bioprocess Laborat ory. Chlorella

sp and Spirulina w ere collect ed from BBPAP Jepara and cult ivat ed w hich w as cont aining Spirulina and Chlorella.

The Chlorella and Spirulina w ere cult ivat ed in different POM E concent rat ion (20%, 50%, and 70% ) and different urea concent rat ion (0.1gr/ l, and 1gr/ l). Light int ensit y w as m aint ained in 3000 lux, pH 6.8-7.2, 280C t em perat ure, and aerat ed using air pum p t o m ix t he m edium in 2 L flask disk glass. The biom ass concent rat ion was m easured by using spect rophot om et er.

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w as calculat ed using biom ass produced divided by cult ivat ion t im e.

3. Result and Discussion

3.1. Wild algae

The wild algae w as grow n w ildly in an open t ank, w it hout addit ion of ext ernal nut rient s. The m ost species appeared in t he cult ure w ere Chlorella and Scenedesmus. The grow t h of w ild algae

w as evaluat ed under POM E m edium (Table 3) Chlorella and Scenedesm us have been recognized as

m icroalgae t hat produce lipid cont ent bet w een 20-30%.

3.2. Biomass vs Opt ical densit y for algae

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Figure 2. Correlat ion bet w een OD and Dry M ass Chlorella sp

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M a ta ra m , July 4-7th₂₀₁₂ << Back t o t able of cont ent s

Table 5. Concent rat ion carbon, nit rogen, and phosphat e in POM E

in dif ferent concent rat ion POM E 1980) w it h recom mended rat io of 56:9:1. According t o equat ion of POM E aft er adding urea, CNP rat io has changed (Table 6):

Table 6 CNP ratio in POM E after additional urea

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Figure 3. Grow t h phase Chlorella sp at 1gr/ l (a) and 0.1

gr/ L (b) urea in diff erent POM E concent rat ion

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vulgaris, Chlorella pyrenoidosa, Bot rycoccus sudet icus, Tet raselmis sp). The result s in Specific grow t h rat e were 0.099/ day, 0.084/ day,

0.048/ day, 0.083/ day and 0.065/ day, respect ively. In t he research, dilut ed POM E could have low er t annic acid, so it did not inhibit phot osynt het ic react ion. Com pared by biom ass product ion, t he result show ed t hat highest biom ass can reached in 8.0 m g/ l/ day. In t his experim ent , highest biom ass w as reached in 58.4 m g/ l/ day. POM E concent rat ion m ay lim it in biom ass form ing, due t o high carbon, nit rogen, and phosphor source.

According t o t his research, higher urea concent rat ion also influences in specific grow t h rat e of Chlorella sp. Urea influences in Chlorella `s grow t h (El Sayed et al., 2011) and t he concent rat ion

also inf luences in biom ass product ion (Choochot e et al., 2010, M andalam & Palsson, 1998). W ijanarko (2011) in his research also st udied influences urea as nit rogen source and conclude t hat urea has pot ent ial nut rient for Chlorella t han nit rat e. It also can increase

grow t h rat e and biom ass product ivit y. The biom ass form ing

Chlorella vulgaris based t he research is:

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Wijanarko (2011) also report ed t hat high urea concent rat ion t hat concent rat ion, and urea concent rat ion.

Acknow ledgement

This research is part of project Indonesian Aquat ic Biom ass, w it h consort ium mem bers: PT Wirana, M aris Project BV and KU Leuven belgium

References

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Choochot e, W eena., Paiboonsin, Kerkkiat ., Ruangpan, Siripong., and Pharuang, Akkaphop. 2010. Effect s of Urea and Light Int ensit y on t he Grow t h of Chlorella sp. The 8t h Int ernat ional

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M andalam , R.K., Palsson, B.Ø., 1998. Elem ent al balancing of biom ass and m edium com posit ion enhances grow t h capacit y in high-densit y Chlorella vulgaris cult ures. Biot echnol. Bioeng. 59,

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