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ABSTRAK
Isolasi senyawa flavonoida yang terkandung di dalam daun tumbuhan Jambu Air (Syzygium aquea (Burm.f.) Alston ) dilakukan dengan ekstraksi maserasi dengan pelarut metanol. Ekstrak metanol dirotarievaporator dan diuapkan hingga seluruh metanol menguap lalu dilarutkan dengan etil asetat kemudian dipekatkan dan
diuapkan. Ekstrak etil asetat dilarutkan dengan metanol dan diekstraksi partisi dengan n-heksana. Fraksi metanol diuapkan dan dihidrolisa dengan HCl 6% sambil
dipanaskan, kemudian filtrat diekstraksi partisi dengan kloroform, Ekstrak pekat kloroform merupakan flavonoida total kemudian dianalisis KLT, lalu dipisahkan dengan kromatografi kolom dengan fase diam silika gel dan fase gerak n-heksana : etil asetat (80:20 v/v) dan dimurnikan dengan preparatif menghasilkan kristal kuning sebanyak 7 mg dengan Rf = 0,35. Selanjutnya diidentifikasi dengan spektrofotometer UV-Visible, Inframerah (FT-IR), dan spektrometer Resonansi Magnetik Inti Proton (H1-NMR). Hasil analisis data dari interpretasi spektroskopi diduga bahwa senyawa yang diisolasi adalah Flavonoida golongan Flavanon.
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ISOLATION OF FLAVONOIDA COMPOUND FROM LEAVES OF JAMBU AIR (Syzygium aquea (Burm.f.) Alston)
ABSTRACT
Isolation of flavonoid compounds from leaves of Jambu Air (Syzygium aquea (Burm,f.) Alston) has been done by maceration technique with methanol solvent. Methanol extract obtained was evaporated and dissolved with ethyl acetate. The solution was than concentrated and evaporated. Ethyl acetate fraction was dissolved with methanol and partitioned with n-hexane solvent. Methanol layer was evaporated and then acidified with HCl 6% while heated, It was then extract partition with chloroform. The concentrated chloroform extract, which is total flavonoid was analysed with thin layer chromatography and then separated using Column Chromatography with silica gel as the stationary phase and n-hexane : ethyl acetate (80:20 v/v) as the mobile phase. The compound was purified with TLC preparative yielding yellow crystal with weight 7 mg with Rf= 0,35. The compound was further identified by spectroscopy analysis using UV-Visible, FT-IR and 1-H-NMR. Spectroscopy data was estimated as flavonoida is flavanone.
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