View of Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp.

Volume 4, Number 2, August 2010 p 84-89 ISSN 1978-3477

I N D O N E S I A

*Corresponding author, Phone/Fax: +62-21-7560208;

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Microbiol Indones

Table 1 Eight isolates ofActinomycetes(Banten West Java Coast) producing antimicrobial active

Isolate Sample

treatment

Diameter of inhibition (mm)

Escherichia coli Staphylococcus aureus Bacillus subtilis Pseudomonas aeruginosa Candida albicans Aspergillus niger

A64 HS - - - 15.00

A610 A - 12.00 - - - -

A11 HS 18.00 15.00 14.00 14.00 - -

A21 HS - - - 7.00 - 9.00

A32 HS - 12.00 - - 7.00 -

A43 A 10.16 - 8.67 9.51 - -

A44 A - - - - 10.61 -

A54 HS - 8,56 - - 8.67 -

Positive control rifamp icin 500 ppm nystatin 1000 ppm

23.52 9.16 15.98 15.18 - -

- - - - 14.84 16.38

HS, heat shock treatment; A, acid treatment; Diameter of paper disc 6 mm

A11

Streptomyces parastreptomyces abscessus Streptomyces streptoallomorpha polyantibiotica

Streptomyces microfla

vus strain HBUM174133 Streptomyces a fricanus

Streptomyces microflavus Streptomyces paresii Streptomyces afghaniensis Streptomyces roseoviolaceus Streptomyces kitasatospora Streptomyces streptacidiphilus Streptomyces sp Ls247 Nocardioides the rmolilacinus Streptomyces malaysiensis

Nocardia abscessus Streptomyces sp QM- B814

Candidatus streptomyces philanthi biovar Streptomyces indonesiensis

Streptomyces brasiliensis 17

57

31 7

5

100 45

100 99

4 2

51 39

18

8

19

24 2

Streptomyces tanashiensis subsp.

cephalomyceticus

Actinomadura Fig 1 Polygenetic tree of isolate A11 shown asStreptomycessp.

Table 2 Biological activity of biomass and supernatant extract from isolate A11

Sample Diameter of inhibition/clear zone (mm)

Staphylococcus aureus

Biomass extract - - - -

Supernatant extract 10.39 24.43 9.64 9.55 - -

Positive control (rifampicin 500 ppm) 21.27 44.57 10.08 10.12 - -

0.00 0.20 0.40 0.60 0.80 1.00 1.20

Time (min)

0 5 10 15 20 25 30 35 40 45 50 55

Fig 2 Analysis HPLC chromatogram of supernatant extract.

0.00 0.10 0.20 0.30 0.40 0.50 0.60

2 4 6 8 10 12 14 16 18

Time (min)

Fig 3 Analysis HPLC chromatogram of biomass extract.

0.00 0.50 1.00 1.50 2.00 2.50

2 4 6 8 10 12 14 16

4.970 6.105 8.691 9.603 10.111 10.641 11.528 12.01012.241 13.283 13.843

Active fraction

Time (min)

Fig 4 Chromatogram of preparative HPLC.

0.00 1.00 2.00 3.00

0 5 10 15 20 25 30 35

8.623

Time (min)

Fig 5 Analysis HPLC chromatogram of active fraction.

Pseudomonas aeruginosa Candidaalbicans Aspergillus niger

Bacillus subtilis Escherichia coli

Absorbanceunit AbsorbanceunitAbsorbanceunitAbsorbanceunit 8.623

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Microbiol Indones

0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40

250 300 350

274.5

(nm)

Fig 6 UV visible spectrum of active fraction.

Absorbanceunit