*Corresponding author, Phone/Fax: +62-251-8314371;
E-mail: sajuthi@indo.net.id
Fig 1 The days of viremia in pigtail challenged with DEN viruses.
Viral detection methods were C6/36 isolation, mosquito inoculation, and RT-PCR. Each bar represents days of viremia from individual animal.
C6/36 Isolation
DEN 4
DEN 3
DEN 2
DEN 1
Days post-injection
0 1 2 3 4 5 6 7 8 9 10
Days post-injection Mosquito Inoculation
DEN 4
DEN 3
DEN 2
DEN 1
0 1 2 3 4 5 6 7 8 9 10
RT-PCR Detection
DEN 4
DEN 3
DEN 2
DEN 1
Days post-injection
0 1 2 3 4 5 6 7 8 9 10
1243 1229 1248 1236 1231 1234 1244 1242 1247 1238 1246 1237 1241 1228 1233 1230
Fig 2 IgM ( ), IgG ( ), and avidity responses ( ) after primary infection and secondary infection . Bars represent the average and standard deviation.
(a) (b)
Fig 3 Anti-DEN IgG subclasses after primary infection with DEN-4.
Total IgG ( ), IgG 1( ), IgG 2 ( ), IgG 3 ( ), IgG 4 ( ).
Primary Secondary
Animal
Number Day 14 Day 28 Day 0 Day 14 Day 28
DEN-1
1230 >640 320 160 >640
1233 >640 160 160 >640
1228 >640
1241 320
DEN-2
1237 >640 320 160 320 320
1246 >640 >640 160 >640 320
1238 >640 320
1247 >640 >640 DEN-3
1242 320 320 >640 >640 160
1244 >640 80 >640 >640 >640
1234 320 20
1231 160 20
1245 320 40
DEN-4
1236 >640 320 320 >640 160
1248 160 160 160 >640 160
1229 320 40
1243 > 640 40
Table 1 Homologous anti-DEN neutralizing antibody responses after primary and secondary infections
IgMandIgGindex
a 7
6
5
4
1 2 3
0
0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0
Avidityindex
Day 0 Day 14 Day 28 Day 87
IgMandIgGindex
b 7
6
5
4
1 2 3
0
1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2
0
Avidityindex
Day 0 Day 14 Day 28 Day 87
0.1
Optical 1
0.5
0
Day 14 Day 28 Day 87
