Paenibacillus yonginensis Abstract 2014

MicrobiologicalResearch172(2015)7–15

ContentslistsavailableatScienceDirect

Microbiological

Research

jo u r n al ho me p a g e :w w w . e l s e v i e r . c o m / l o c a t e / m i c r e s

Paenibacillus

yonginensis

DCY84

T

induces

changes

in

Arabidopsis

thaliana

gene

expression

against

aluminum,

drought,

and

salt

stress

Johan

Sukweenadhi

a,1

_,

_Yeon-Ju

_Kim

a,∗,1

_,

_Eul-Su

_Choi

b

_,

_Sung-Cheol

_Koh

c

_,

_Sang-Won

_Lee

d

_,

Yu-Jin

Kim

a

_,

_Deok

_Chun

_Yang

a,e,∗

a_{DepartmentofOrientalMedicinalMaterial&Processing,KyungHeeUniversity,RepublicofKorea}

b_{GuyeonggiDoAgriculturalResearch&ExtensionServices,Gyeonggi-do,RepublicofKorea}

c_{DepartmentofEnvironmentalEngineering,KoreaMaritimeandOceanUniversity,Busan,RepublicofKorea}

d_{DepartmentofPlantMolecularSystemsBiotechnologyandCropBiotechInstitute,KyungHeeUniversity,RepublicofKorea}

e_{GraduateSchoolofBiotechnologyandGinsengBank,KyungHeeUniversity,RepublicofKorea}

a

r

t

i

c

l

e

i

n

f

o

Articlehistory:

Received11December2014

Receivedinrevisedform9January2015 Accepted11January2015

Availableonline19January2015

Keywords:

Plantgrowth-promotingbacteria

Paenibacillus

Saltstress Droughtstress Aluminumstress

a

b

s

t

r

a

c

t

Currentagriculturalproductionmethods,forexampletheimproperuseofchemicalfertilizersand pesti-cides,createmanyhealthandenvironmentalproblems.Useofplantgrowth-promotingbacteria(PGPB) foragriculturalbenefitsisincreasingworldwideandalsoappearstobeatrendforthefuture.Thereis possibilitytodevelopmicrobialinoculantsforuseinagriculturalbiotechnology,basedonthese benefi-cialplant–microbeinteractions.Forthisstudy,tenbacterialstrainswereisolatedfromYonginforestsoil forwhichinvitroplant-growthpromotingtraitscreenings,suchasindoleaceticacid(IAA)production, aphosphatesolubilizationtest,andasiderophoreproductiontestwereusedtoselecttwoPGPB candi-dates.ArabidopsisthalianaplantswereinoculatedwithPaenibacillusyonginensisDCY84T_{andMicrococcus}

yunnanensisPGPB7.Saltstress,droughtstressandheavymetal(aluminum)stresschallengesindicated thatP.yonginensisDCY84T_{-inoculatedplantsweremoreresistantthancontrolplants.AtRSA1,AtVQ9and}

AtWRKY8wereusedasthesalinityresponsivegenes.TheAtERD15,AtRAB18,andAtLT178wereselected tocheckA.thalianaresponsestodroughtstress.AluminumstressresponsewascheckedusingAtAIP, AtALS3andAtALMT1.TheqRT-PCRresultsindicatedthatP.yonginensisDCY84T_{canpromoteplant}

tol-eranceagainstsalt,drought,andaluminumstress.P.yonginensisDCY84T_{alsoshowedpositiveresults}

duringinvitrocompatibilitytestingandvirulenceassayagainstX.oryzaepv.oryzaePhilippinerace 6(PXO99).BettergerminationratesandgrowthparameterswerealsorecordedfortheP.yonginensis DCY84T_{ChuchungcultivarriceseedwhichwasgrownoncoastalsoilcollectedfromSuncheon.Basedon}

theseresults,P.yonginensisDCY84T_{canbeusedasapromisingPGPBisolateforcropimprovement.}

©2015ElsevierGmbH.Allrightsreserved.

1. Introduction

Plantgrowth-promotingbacteria(PGPB)includespecieswhich formspecificsymbioticrelationshipswithplants(e.g.,Rhizobiaspp. andFrankiaspp.),thosewhicharefree-living,bacterialendophytes whichcancolonizesomeoraportionofaplant’sinteriortissues, andcyanobacteria(Bashanetal.2004).PGPBarebacterialstrains isolatedfromdiverseenvironmentswhichareabletobeneficially

∗ Corresponding authors at: Department of Oriental Medicinal Material& Processing,KyungHeeUniversity,RepublicofKorea.Tel.:+82312012100; fax:+82312022688.

E-mailaddresses:yeonjukim@khu.ac.kr(Y.-J.Kim),deokchunyang@khu.ac.kr

(D.C.Yang).

1 _{Theseauthorscontributedequallytothiswork.}

affectmanyparametersofplantgrowthandyield,directlyor indi-rectly(Diaz-ZoritaandFernandez-Canigia2009).Plantgrowthis directlypromotedbyPGPBeitherbyfacilitatingnutritionuptake orbymodulatingtheplanthormonelevels.Indirectpromotionof plantgrowthoccursviaPGPBsdecreasingtheinhibitoryeffects ofvariouspathogenicagentsonplantgrowthand development (i.e.,actingasabiocontrolagent).Theprotectionistypically mani-festedasbothareductioninabioticstresssymptomsandinhibition ofpathogengrowth(bioticstress),which canbephenotypically similartopathogen-inducedsystemic acquiredresistance(SAR) or inductionof systemic resistance(ISR,Ross1961).ThisPGPB effecthasbeendemonstratedin differentplantspecies,suchas bean, carnation, cucumber, radish, tobacco, tomato, and in the modelplantA.thaliana(VanLoonetal.1998).Althoughitiswell knownthatSARorISRtriggeredbyPGPBconfersresistanceagainst pathogen-inducedplantdiseases,afewpublishedreportssuggest