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LAMPIRAN
Lampiran1 Penghitungan% kematian dan nilai LC50 BSLT ekstrak kasar metabolit bakteri yang berasosiasi dengan spons
HAL-74
Konsentrasi ekstrak
(µg/mL) Log
konsentrasi Rata-rata %
kematian Rata-rata probit
0 0 0 0
10 1 0 0
100 2 15 3.94
250 2.39 43.33 5.36
500 2.69 100 8.09
1000 3 100 8.09
HAA-01
Konsentrasi ekstrak
(µg/mL) Log
konsentrasi Rata-rata %
kematian Rata-rata probit
0 0 0 0
10 1 0 0
100 2 6.66 2.24
250 2.39 13.33 3.88
500 2.69 36.66 4.65
1000 3 100 8.09
HAL-13
Konsentrasi ekstrak
(µg/mL) Log
konsentrasi Rata-rata %
kematian Rata-rata probit
0 0 0 0
10 1 6.66 3.48
100 2 16.66 4.02
250 2.39 40 4.75
500 2.69 58.33 5.21
1000 3 100 8.09
Contoh penghitungan persentase kematian larva A. salina Jumlah larva awal (C) = 20
Jumlah larva mati perlakuan (A) = 8 Jumalah larva mati pada kontrol (B) = 0
% kematian = x 100%
= x 100 %
= 40 % Contoh Penghitungan LC50
y = 3.032x - 1.352
-2 0 2 4 6 8 10
0 1 2 3 4
Mortalitas Probit
Log 10 Konsentrasi