No. Time Code Name Title
1. 13.00-13.15 ABS-97 Zarani Mat Taher
Optimization, Scaling-up and establishment of herbal processing technology for the
production of bioactive extracts from selected Malaysia medicinal plant 2. 13.15-13.30 ABS-18 Neneng Siti
Silfi Ambarwati
In Silico Molecular Docking Study of Isolated Compounds
from Clusiaceae Family as SARS-COV2 Main Protease
Inhibitor
3. 13.30-13.45 ABS-40 Annisa Auliya Electrodeposition of Gold Nanoparticles from a Low Electrolyte Concentration and
Its Application for Ethanol Electrooxidation 4. 13.45-14.00 ABS-50 Kurniawanti Antioxidant Activities of
Biflavonoids from Indonesia Araucaria columnaris Leaves
5. 14.00-14.15 ABS-53 Wisnu
Widikdo
Optimization of Silver Nanoparticle Synthesis Using Fingerroot (Curcuma rotunda
L.) Rhizome Extract as a Bioreduction agent 6. 14.15-14.30 ABS-69 Syifa Aulia
Mumpuni
Microwave Assisted Synthesis of Coumarin 3-carboxylate
Through Knoevenagel Condensation with Aluminum
Chloride Catalyst 7. 14.30-14.45 ABS-70 Nabila Seflini Synthesis of Chalcone
Derivatives from Vanillin and Acetophenone
8. 14.45-15.00 ABS-71 Pandu Rizki Triananda
Synthesis Of A Chalcone Derivative From Vanillin Using Microwave Irradiation
Method
9. 15.00-15.15 ABS-73 Reyhan Syifa Synthesis 3-Acetyl Coumarin via Knoevenagel Condensation
Under Microwave Irradiation
56
10. 15.15-15.30 ABS-96 Dandy Akbar Juliansyah
grxB gene as a potential target for the development of a
Cronobacter sakazakii detection method in infant
formula milk using PCR 11. 15.30-15.45 ABS-98 Jefferson
Lynford Declan
The Potential of inlA Gene as a Target Detection for Listeria monocytogenes in Vegetables
Sample using Polymerase Chain Reaction Method 12. 15.45-16.00 ABS-99 Ismaya
Krisdawati
The Potential of The ToxR Gene As A Detection Tool for
Vibrio parahaemolyticus in Seafood
13. 16.00-16.15 ABS-184 Fera Kurniadewi
Structure- antioxidant Activity Relationship of Flavonoids
from Three Species of Indonesian Artocarpus 14. 16.15-16.30 ABS-211 Nurul Nadiah
Abu Bakar
Optimization of roselle (Hibiscus sabdariffa) anthocyanin extraction parameter by response surface
modeling and comparison of anthocyanin content from calyces, stems, leaves and
calyces residue.
57
58
59
Genetic Diversity, Heritability and Path Analysis of Sweet Cassava Promising Clones Sholihin
IAARD / BRIN, Jakarta, Indonesia
Abstract
The goal of the trial was to determine the genetic diversity of sweet cassava promising clones and to measure the heritability and the closeness of relationship between morphological trait and tuber and starch yield. Place of trial was in Kalipare, Malang, Indoensia establish in 357 m from see level with Entisol soil. The trial was carried out during 2018 with a RCBD, 3 replications. Plants distance was a 100 cm x 80 cm. Twelve promising clones and released variety as check were tested in this trial. The results showed that the genetic variability for fresh tuber yield, starch content, long of tuber, diameter of tuber, tuber number/plant in eight months and plant height in 4 months. Degree of the genotypic correlations was higher than the phenotypic correlations. The fresh tuber yield was positively and significantly correlated with tuber number/plant and tuber long genetically and phenotypically and tuber diameter genetically. The fresh tuber yield was negatively significantly correlated with starch content and plant height in 4 months genetically and phenotypically. Tuber diameter had largest direct effect on fresh tuber yield genetically. High broad sense heritability estimates were recorded in tuber diameter, tuber long, starch content, fresh tuber yield.
Keywords: Genotypic diversity, heritability, path analysis, sweet cassava promising
60
Topic : Biology [ABS-39]
Correlation Between Macroalgae Abundance with Echinodermates at Sepanjang Beach, Gunung Kidul-Yogyakarta
Ratna Komala, Hanum Isfaeni, Muhamad Rizal, Dwi Angelita ,Bina Rahayu Setyasih Master of Biology Education Faculty of Mathematics and Natural Sciences-Jakarta State
University, Indonesia
Abstract
Macroalgae and echinoderms are marine biological resources whose presence can be found in the intertidal zone. One of the beaches that supports the life of macroalgae and echinoderms is the Sepanjang Beach. These beach is generally rocky coastal waters overgrown with various macroalgae and home to various types of echinoderms such as the Ophiuroidea (snaking stars), Echinoidea (sea urchins) and Holothuroidea (sea cucumbers) groups. The existence of tourism activities on the Sepanjang Beach which is open to the general public has an indirect impact on macroalgae and echinoderms. Therefore, the purpose of this study was to determine the abundance, diversity, dominance and correlation between the abundance of macroalgae and echinoderms on the Sepanjang beach. The research method uses a descriptive method with direct observation. Data analysis was carried out descriptive through the calculation of abundance, diversity, dominance and statistically through the calculation of Pearson correlation. Based on the results of the correlation coefficient at the observation station at Sepanjang Beach, it shows a significant positive correlation between the abundance of macroalgae and echinoderms.
Keywords : Echinoderms, Macroalgae, Sepanjang Beach
61
Vegetation Analysis and Diversity of Seed Plants (Spermatophyta) in the Area Around Porok Beach, Yogyakarta
Rizhal Hendi Ristanto 1,a), Khalida Zia 2),Helda Dumayanti 3,b), Clavinzky Anggita Pasaribu 4) and Mieke Miarsyah5)
12345) Department of Biology Education, Faculty of Mathematics and Sciences State University of Jakarta, Indonesia
a) Corresponding author: [email protected] b)[email protected]
Abstract
Seed plants (Spermatophyta) as biodiversity need to be preserved because they have many benefits. This study aims to determine the analysis of vegetation & diversity of seed plants (Spermatophyta) in the Porok Beach Forest, Yogyakarta. The research method used is a survey method by direct observation and quantitative descriptive of the vegetation area. The research instrument is in the form of observation sheets for the analysis of vegetation and diversity of seed plants (Spermatophyta). The population in this study is all seed plants (Spermatophyta) found in the Porok Beach Forest, Yogyakarta. The sample in this study is seed plants (Spermatophyta) with tree habitus, taken by purposive sampling on a quadratic transect. This type of research is used in line transect and quadratic transect methods. The research design is made with a 70 m long transect line in the tree vegetation area. On the transect line, 3 plots/square are made with a plot size of 10 m x 10 m and a distance between plots of 10 m.
Each plot/square was repeated 3 times, so there were 9 plots/square. Vegetation analysis is carried out by calculating Important Value Index (INP), Shannon-Wienner diversity index (H^), and environmental parameters. Based on the results of research on seed plants (Spermatophyta) that has been carried out in the area around Porok Beach, Yogyakarta with tree habitus found as many as 5 families, 5 species and 33 individuals. There were 5 (five) types of vegetation composition, namely Casuarina equisetifolia (Cemara Udang), Terminalia catappa (Ketapang), Pandanus odorifer (Sea pandanus), Cocos nucifera (Coconut) and Phempis acidula (Stigi). The species diversity in the area around Porok Beach, Yogyakarta is classified as moderate, namely 1.49 which has a diversity index of 1 < H^ 3 where the diversity index is moderate.
Keywords : Vegetation Analysis, Diversity, Porok Beach, Seed Plants
62
Topic : Biology [ABS-49]
Gastropod Abundance and Diversity at Panjang Beach, Yogyakarta Hanum Isfaeni, Rusdi, Reza Dino Mahardika, Risda Putri Indriani, Feni Oetari
Universitas Negeri Jakarta
Abstract
Sepanjang Beach Beach is located in Gunungkidul Regency, Yogyakarta. This beach is composed of coral reefs which are one of the habitats of mollusks, including gastropods.
Gastropods in the ecosystem act as decomposers and provide micro-ecosystem nutrients for microorganisms. Gastropods also play a role in the food chain in coastal ecosystems. Gastropod diversity and abundance can affect this. So it is necessary to analyze the diversity and abundance index of gastropods in coastal ecosystems. This study uses an exploratory descriptive method with data collection techniques using quadratic transects. The data obtained were analyzed for abundance and diversity index.
Keywords: Mollucs, Quadrant Transect, Sepanjang Beach
63
Coastal Plants Transpiration of Porok Beach Yogyakarta, Indonesia Supriyatin*, Sri Rahayu, Anggi Putri Suhadi, Remli Nelmian Simarmata, Alfi Lailatul
Qodriyah
Department of Biology Education, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia
Abstract
Water vapor collection is one of the measurements of transpiration. The characteristics of coastal forests with strong winds, full light intensity, and sandy soil will affect plant transpiration, under these conditions plants have their own way to grow and adapt to the environment. This study aims to analyzing transpiration of coastal forest plants using the method of collecting water vapor. Descriptive method is applied by measuring amount of water vapor, leaf surface area, number and density of stomata, wind speed, temperature, air, humidity, light intensity and salinity. Sample used is determined using purposive sampling of coastal forest plant along Porok beach, Gunung kidul, DIY, three types of plants were obtained with the categories of small single leaf, large single leaf and compound leaf, samples were taken based on the plant part (top, middle, bottom). Data is analyzed with quantitative descriptive technique, using tables of observations, graphs of transpiration development in three different time periods, photo documentation of observations and statistical analysis on leaf area measurements, and stomatal density
Keywords: Transpiration, Measurement, Coastal Forest, Plants
64 Topic: Biology
[ABS-56]
Different Sources of Diet To Captive Sulphur-Crested Cockatoo (Cacatua galerita) On Nutrient Digestibility And Blood Cholesterol
Sinta Maharani* 1,2), Rita Mutia 2), Siti Nuramaliati Prijono1), Rini Rachmatika1), Tri Hadi Handayani1), R. Lia Rahadian Amalia1), Suparno1)
1)Research Center of Applied Zoology, National Research and Inovation Agency (BRIN) 2)Faculty of Animal Science, IPB University, Indonesia
Abstract
Captive bird diets are commonly based on offering vegetables and fruits. This research aimed to analyze the varied diets of seeds, fruits and vegetables, and the addition of commercial pellets on nutrient digestibility, apparent metabolic energy, and blood cholesterol. The study was conducted using 12 C.galerita consisting of six males and six females in Bird Captivity, LIPI for six weeks. Feed treatment for the birds consisting of control diet (P0), control diet + 50%
pellet (P1), control diet + 75% pellet (P2). The design used in this study is a randomized block design (RBD). The use of additional pellets on sulphur-crested cockatoo diet shows a significant effect (P>0.05) on crude protein, crude fiber, and ether extract nutrient digestibility.
The digestibility of dry matter, crude protein, and crude fiber at P0 were smaller than P1 and P2, while the digestibility of crude fat in P0 was higher than P1 and P2. P2 treatment with pellet addition was 75% higher than treatment P0 and P1, namely 1912.98 cal kg-1. The lowest apparent metabolic energy was treated P0 with EMS 1826,83 cal kg-1. The addition of pellet to the sulphur-crested cockatoo diets did not show a significant effect (P>0.05) on the total cholesterol, HDL, LDL, and triglyceride levels C.galerita.
Keywords: Cacatuidae, captivity, cholesterol, nutrition, parrot diet
65
Single Rearing by Female on Sulphur Crest Cockatoo (Cacatua Galerita, Latham 1790) Rini Rachmatika*1), Sinta Maharani1), Siti Nuramaliati Prijono1)
Applied Zoology Research Center, National Research and Innovation Agency (BRIN)
Abstract
Parrots are often systematically hand-reared to satisfy the pet trade demand. Success breeding can not be separated from the feed and behavioral aspects. Behaviour of Cacatua galerita is necessarily observed to support captive breeding in Research Facility for Bird Captive Breeding, National Research and Innovation Agency (BRIN). The study aim to saw sulphur crest cockatoo^s behaviour during the rearing season without males. Observation of activity and feed intake have been conducted during two periods of rearing season. Activity observation for seven days/season and feed intake for 20 days/season. The feed is given consisting of boiled egg, bird cake, carrot (Daucus carota), fresh corn (Zea mays), guava (Psidium guajava), kale (Ipomoea aquatica), coconut (Cocos nucifera), peanut (Arachis hypogaea), ambarella (Spondias dulcis) mung bean sprouts (Spigna radiata), yardlong bean (Vigna unguiculata subsp.
sesquipedalis). Method of behaviour using focal animal sampling. The result showed nesting behaviour is the highest duration (41.77%) of the rearing season by a female. The highest feed intake is fresh sweet corn as carbohidrat source
Keywords: behaviour, breeding, parrot, cacatuidae
66 Topic: Biology
[ABS-64]
Characterization of Amylolytic Bacteria Isolated from Macroalgae at Sepanjang Beach, Yogyakarta
Tri Handayani Kurniati1, Evita Nury Hariyanti2, Rizki Awalia2, Sudaryanti2, Ratna Komala1
1) Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta. Jl. Pemuda No. 10 Rawamangun, East Jakarta, Indonesia 13220
2) Master^s Degree of Biology Education Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta. Jl. Pemuda No. 10 Rawamangun, East Jakarta,
Indonesia 13220
Abstract
Enzymes are important components in microorganisms. One of the enzymes commonly produced is amylase. Amylase enzymes have various uses, such as basic material in the food and drug industry, the textile industry and other industries. Amylolytic bacteria has the ability to hydrolyze starch or into simpler compounds. This study aims to obtain amylolytic bacteria associated with macroalgae in Sepanjang Beach, Gunungkidul, Yogyakarta. The 3 macroalgae samples studied were Chaetomorpha linum, Eucheuma spinosum, and Enteromorpha sp.. There were 10 colony bacteria isolated from Chaetomorpha linum, 8 colony from Eucheuma spinosum, and 8 colony from Enteromorpha sp.. Isolation of bacteria using pour plate method on Marine Agar medium with incubation for 24 hours. Selection of amylolytic bacteria was carried out on 2% Starch Agar medium. A positive result was indicated by the presence of a clear zone around the colony after added iodine reagent. The amylase production ability of each bacterial isolate was tested by measuring the Amylolytic Index. Characterization of bacterial isolates was carried out based on observations of colony morphology (shape, size, color, edge, and elevation), as well as cell morphology which included cell shape and Gram type.
Amylolytic index data were analyzed quantitatively using Duncan^s multiple-region union, and data on the characteristics of bacterial isolates were analyzed qualitatively.
Keywords: Amylolytic bacteria, characterization, macroalgae
67
Screening and Identification of Exopolysaccharide Producing Bacteria From Pickled Fruit
Tri Handayani Kurniati, Noer Syahbani, Sri Rahayu
Biology Department, State University of Jakarta, Jakarta Timur, 13220, DKI Jakarta, Indonesia
Abstract
Exopolysaccharides (EPS) are biopolymers in the form of carbohydrate chains that synthesized and secreted by bacteria outside the cell. EPS can be widely used in various industrial fields.
This study aims to obtain isolates of EPS-producing bacteria from pickled fruit and determine their identity based on 16S rRNA sequence analysis. The method used in this research was descriptive and experimental methods. The research stages consist of isolation, screening, characterization, identification, and ability assay of producing EPS. Based on screening results on MRSA media enriched with skim milk, as many as 15 bacterial isolates from pickled fruit with various morphological characteristics were able to produce EPS which was characterized by the formation of white zones and mucoid colonies. Three bacterial isolates, namely A4, M1, and P4 were known to have an EPS index value of 0.43, 0.33, and 0.30 and had a relatively fast growth compared to other isolates. Analysis of 16S rRNA sequences showed that the isolates of EPS-producing bacteria from pickled fruit were identified as Peribacillus acanthi A4 (99.69%), Priestia flexus M1 (99.15%), and Priestia megaterium P4 (99.77%). The results ability assay of producing EPS showed that Peribacillus acanthi A4, Priestia flexus M1, and Priestia megaterium P4 had the ability to produce EPS (ppm) sequentially as much as 847, 1,013.57, and 1,007.29. The results of one way ANOVA showed that there was a significant effect of bacterial type on the ability to produce EPS with a significance (P<0.05). DMRT 5%
test showed there were two isolates that had no significant difference in ability to produce EPS, namely Priestia flexus M1 (1,013.57 ppm) and Priestia megaterium P4 (1,007.29 ppm). The results of this study provide information on EPS producing bacteria from pickled fruit that can be developed as gelation, bioremediation, and immunomodulators agents.
Keywords: Exopolysaccharide, Pickled fruit, Peribacillus acanthi, Priestia sp., Screening
68 Topic: Biology
[ABS-78]
Masigit as a habitat for endemic bird species and its potential risk of land degradation Ratna Komala, Fathurrohman, Isnin Noer
Jakarta State University
Abstract
Indonesia has the most significant endemic bird population in the world. Java island is a place for some important endemic species. This is the result of the transformation from the ^old^
bloom taxonomy to a ^new^ species distribution that becomes more narrow. The immense human activity in the Java forest endangers biodiversity that causes forest degradation. This research aimed to determine the condition and forest degradation level based on the average value of NDVI, composed of bird species, population density, and the endemic birds^
community variation on Java reviewed from the degradation forest level. This research was taken from November to December 2021 in the north Masigit Mountain (Cibanggala) and south (Ci Lumut) area using the descriptive method and Line Transect technique. Moreover, the procedure was conducted to decide the research location and bird species for observation. The result showed that Ci Lumut forest does not show forest degradation on the other side part of Cibanggala forest showed quite a high forest degradation. The highest composition and the composed Javanese endemic bird species in Ci Lumut were Psilopogon corvinus (17% and 6,5 birds/km2), and the smallest was Aethopyga eximia, Alcedo euryzona, Nisaetus bartelsi, Psilopogon javensis, dan Rhipidura euryura (1% and 0,5 birds/km2). The highest composition and the composed in Cibanggala was Eurylaimus javanicus (22% and 6,5 birds/km2) and the smallest was Otus angelinae and Nisaetus bartelsi (1% and 0,5 birds/km2) The results of the PERMANOVA analysis states that the community of Javanese endemic birds in Masigit mountain shows that the differentiation reviewed from the level of degradation forest, kinds of birds, and guild group more in the forests with high vegetation density than in forests with medium-low vegetation density.
Keywords: Birds, Community, Degradation, Endemic.
69
Anti-Cancer Potential of White Turmeric Extract (Curcuma zedoaria) in Mcf 7 and Hepg 2 Cancer Cells
Sri Rahayu1*, Naufal Ma’arif2, Dania Afifah1, Arief Prasetiyo1, Firda Kamila1, Anisa Fitriani2, Alifa Alya Aurora2, Fadila Nuranfa Putri2, Annisa Wulan Agus2 1Departemenet of Biology, Faculty of Mathematics and Natural Science, Jakarta State
University, Indonesia
2Departemenet of Biology Education, Faculty of Mathematics and Natural Science, Jakarta State University, Indonesia
*Corresponding email: [email protected]
Abstract
White rhizome (Curcuma zedoaria) is a plant from the Zingiberaceae family that has an advantage in medicine and cancer therapy. This research aims to find the cytotoxic level of white turmeric in the MCF-7 and HepG2 cell lines. The method used in this research is experimental with a completely randomized design. The treatment group consisted of 10, 20, 25, and 50 ppm Curcuma zedoaria extract for MTT (Micro tetrazolium) test and the control group (without extract) using inhibitory concentration (IC50). The analysis data used one-way ANOVA and Post-hoc Tests. The results of the one-way ANOVA showed that the significance level of concentration on the MCF-7 cell line was 0.00 < 0.05, indicating that there was a different effect of concentration. The highest inhibition value of the white turmeric rhizome extract was shown at a concentration of 50 PPM with an inhibition level of 61.86%. The significance level of the one-way ANOVA for the HepG2 cell line was 0.02 < 0.05, so there was no change in the effect of concentration on cancer cells. The highest inhibition value in HepG2 cancer cells was 18.83% and lower than the MCF-7 cell line. The results showed that the highest IC50 value for the MCF-7 cell line was 68.58ppm, and the HepG2 cell line was 40 PPM. It can be concluded that white rhizome extract has a cytotoxic effect on MCF-7 and HepG2 cell lines.
Keywords: White rhizome, Anti-Cancer, IC50, MCF7, HepG2.
70 Topic: Biology
[ABS-84]
Screening and Isolation of Indigenous Cellulolytic and Proteolytic Yeasts From Fermented Dried Cocoa Beans
Dalia Sukmawati1,2, Shabrina Nida Al Husna3, Desty Saszieta1, Almira Marvella Priskaningrum1, Axel Mareta Mutiani1, Ariza Budi Tunjung Sari4 and Hesham Ali El-
Enshasy5
1Department of Biology, Faculty of Mathematics and Natural Sciences, Hasyim Ashari Building, 9th floor, Universitas Negeri Jakarta, Jakarta, Indonesia.Dalia-
2Universitas Negeri Jakarta Culture Collection (UNJCC), Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Rawamangun, Indonesia- Dalia-
3Department of Microbiology, School of Life Science and Technology, Institut Teknologi Bandung, Bandung, Indonesia.
4Coffee and Cocoa Research Center Indonesia, Jl. PB Sudirman No.90, Wetan Ktr., Jemberlor, Kec. Patrang, Kabupaten Jember, Jawa Timur 68118, [email protected] 5Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor
Bahru, Malaysia- [email protected] Abstract
Fermentation is a biochemical process that requires various microorganisms. The activity of microorganisms in the cocoa bean fermentation process is essential to successful enzymatic molecule breakdown. Yeast is a functional microorganism in cocoa bean fermentation and acts as a glucose and ethanol producer. Yeast has the ability to produce cellulase and protease enzymes, which are both frequently used in industry. This study aimed to screen and isolate yeast that can degrade cellulose and proteases from the fermentation of Sulawesi 1 and TSH 858 cocoa beans. The study was carried out using the descriptive method. Data were obtained by isolating up to 20 g of yeast from each fermentation. The results showed 231 yeast isolates, including 136 from Sulawesi 1 and 95 from TSH 858. The cellulolytic screening revealed 29 isolates from Sulawesi 1 and 27 isolates from TSH 858, whereas the proteolytic screening discovered three isolates and no isolate from the TSH 858 that produced proteolytic enzymes.
Keywords: screening, yeast isolation, cellulolytic. proteolytic, cocoa bean fermentation