3.9 Column Management
3.9.5 Viewing the Acquisition Log
"Viewing the acquisition log in the column manager"
"Viewing the deterioration status of columns in the column browser"
"Viewing chromatograms in the column browser"
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3.9.2 Setup for LabSolutions
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Setting up LabSolutions
The procedure of this section requires the LabSolutions user permission of [Edit System Configuration].
1 In the [Main] assistant bar, click the [System Configuration] icon.
2 In [Available Modules], select [Column] and then click the [
→] button before clicking the [OK] button.
After a link beep sounds, the [Column] information appears in the [Column Oven] tab.
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Initializing the CMD
The procedure of this section requires the LabSolutions user permission of [Edit System Configuration].
Initialize your CMD before you use it for the first time.
Initialization needs to be done only once.
1 Start the system units.
1
Turn on power to the HPLC units.Each unit starts initialization.
2
Wait for the units to complete initialization.3
After the initialization process, verify that every unit established a link to the system controller (or that the link " " of each unit is on).For details on configuration of each unit, refer to the instruction manual of each unit.
2 Connect the CMD cable to the CMD to be initialized.
"Installing your CMD and column to the instrument" P.108
3 Start the [Realtime Analysis] program of LabSolutions.
"2.1.2 Logging in to LabSolutions and Opening the [Realtime Analysis] Window" P.12
4 In the LabSolutions tool bar, click [CMD Information].
The [CMD Information] window appears.
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5 After the message "A CMD specified in a previous version was inserted. Set the column information again after clearing the CMD information." appears, click the [OK] button.
6 Click the [Clear CMD Info.] button.
7 After the message "Clear the CMD information. Is that OK?" appears, click the [Yes] button.
8 After the message "Set the column information." appears, click the [OK] button.
The initialization of the CMD is complete when the display of [CMD 1 (Oven)] changes from [Previous Version and CMD-Instrument Inconsistency] to [Not registered].
According to "Registering the column in the CMD" P.109, register a new column in the CMD.
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3 Enter column information (column specifications including name, column ID, internal diameter, and length) and then click the [OK] button.
• The column manager is not available for LabSolutions LC/GC and LabSolutions LCMS. Available in LabSolutions DB and LabSolutions CS.
• The column manager is not available for IVDR System.
• Be sure to enter the name and column ID.
• For details on the items, refer to the help of the column manager.
• To replace the column with a new one with equivalent specifications, click the [Add]-[Copy] button, change [Column ID], and then edit necessary items before registering the column.
• Perform this procedure before installing the column to the instrument.
• Exit the column manager as needed. (It is not always necessary to exit the column manager.)
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Installing your CMD and column to the instrument
This section describes installation of a CMD and a column to the instrument.
"7.5.3 Installing Optional Column Management Functionality (Column Management Device or “CMD”)" P.313
1 Connect the CMD cable to the "CMD"
connector of CTO-40C CL.
2 Fix the CMD to the column with a cable tie coming with the CMD.
3 Install the column equipped with the CMD to the column oven.
4 Connect the CMD to the CMD cable.
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Registering the column in the CMD
Initialization updates the device information format of the CMD. Do not use the updated CMD in an environment where an older version of LabSolutions is in use.
1 Start the system units.
1
Turn on power to the HPLC units.Each unit starts initialization.
2
Wait for the units to complete initialization.3
After the initialization process, verify that every unit established a link to the system controller (or that the link " " of each unit is on).For details on configuration of each unit, refer to the instruction manual of each unit.
2 Start the [Realtime Analysis] program of LabSolutions.
"2.1.2 Logging in to LabSolutions and Opening the [Realtime Analysis] Window" P.12
3 In the LabSolutions tool bar, click [CMD Information].
The [CMD Information] window appears.
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4 Click [Column List] and then, in the [Read Column Information] window, select the column you registered during the procedure of "Starting the column manager
and registering a column" P.106, then click the [Read] button.5 Enter supplementary information and comments as needed and then click the [Apply] button.
• For details on the setting items, refer to the help of [CMD Information].
• Be sure to enter the name and column ID.
• Some setting items cannot be registered in CMD. Use the column manager to set the items that cannot be registered.
6 When the data are written successfully, the display of [Not registered]
disappears. After the following message appears, click the [OK] button.
After registration in CMD, the LC status changes to [CMD-Method Column Mismatch]. In the [Column Oven] tab, click the [Read from CMD] button.
7 Click the [Close] button.
The [CMD Information] window closes.
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How to replace a column (CMD) (how to discard a CMD)
This section describes how to replace a column together with the CMD after the separation capability deteriorates.
1 Connect the CMD of the column to be discarded to the CMD cable.
2 In the LabSolutions tool bar, click [CMD Information].
The [CMD Information] window appears.
3 Click the [Discard Column] button.
4 After the message "Set the column information." appears, click the [OK] button.
5 Click the [Close] button.
The [CMD Information] window closes.
6 Cut the cable tie to remove the CMD from the column.
7 According to "Installing your CMD and column to the instrument" P.108, fix a CMD to a new column and register it to the CMD.
If the new column has the same specifications as the old one, after selecting the old column, click [Add]-[Copy], which saves you the trouble of data entry.
Be sure to change the column ID at this time.
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How to replace the column (how to discard it using the column manager)
This section describes how to replace (discard) the column without using the CMD.
1 Start the column manager.
2 Select the column to be discarded and then, from the right-click menu, click [Setting Status].
3 In the [Status Setting] window, select [Disposed] and then click the [OK] button.
4 According to "Starting the column manager and registering a column" P.106, register a new column.
If the new column has the same specifications as the old one, after selecting the old column, click [Add]-[Copy], which saves you the trouble of data entry.
Be sure to change the column ID at this time.
3.9.4 Analysis with LabSolutions
This section describes analysis with LabSolutions. The procedure requires the LabSolutions user permission of [Read from CMD] and, for configuring the column using the column manager, the [Edit Method (Instrument Parameters)], [Edit Method (Pump Flow)], or [Column installation to instrument] permission. Also, analysis requires the [Run Single Data Acquisition] permission or [Run Batch Data Acquisition] permission.
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Configuring the column (reading from the CMD)
This section describes configuration of the CMD.If you have used the column (CMD) with another instrument, the [Column] is automatically removed from [Modules Used for Analysis] when the [Realtime Analysis] program starts.
According to the procedure of "3.9.2 Setup for LabSolutions" P.102, in the [System Configuration] window, add the [Column] unit to [Modules Used for Analysis] before performing the procedure of this section.
This procedure requires the LabSolutions user permission of [Edit System Configuration].
1 In the [Column Oven] tab, click the [Read from CMD] button.
After a link beep sounds, the column information is reflected and the [CMD-Method Column Mismatch] status changes to one such as [Ready].
Without the mobile phase purged, the status may change to another such as [Pump A Wait Purge].
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Configuring the column (from the column manager)
This section describes how to configure the column without using the CMD.
1 In the [Column Oven] tab, click the [Settings] button.
2 Click the [Refer to Column List] button.
The [Column List] window appears.
3 Select the column to be used in analysis and then click the [Select] button.
The information on the selected column is reflected.
4 Click the [OK] button.
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Analysis
For details, see "2.7 Performing Realtime Batch (Sequence) Analysis " P.48, "2.8 Stopping Analysis and Shutting OFF the Power Supply" P.55, and "2.9 Key Points for Analysis"
P.58.
3.9.5 Viewing the Acquisition Log
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Viewing the acquisition log in the column manager
You can view analysis results of each column.1 From the column list view, select a column and then click the [Acquisition Log]
menu.
2 View the analysis results of the selected column.
1 Enables you to specify filtering conditions for analysis results.
2 Displays filtered analysis results. For display contents, refer to the help of the column manager.
You can print the results. For details, refer to the "Column Manager Operating Manual"
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Viewing the deterioration status of columns in the column browser
The column browser enables you to view analysis data of each column. The browser displays time series data of the number of theoretical plates of component peak, symmetry factor, resolution, and change in pre-injection pressure.
1 With the history of column analysis results displayed, click the [Column Data Browser] menu.
2 Click the compound name of the component peak you wish to check.
This requires that the compound table be already edited (compounds be already identified) in analysis data. Results are displayed for identified data.
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3 Click the column parameter you wish to view.
Injection time series of change in the parameter appears in the lower section of the window.
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Viewing chromatograms in the column browser
1 Click [Load Chromatogram].
The browser displays the chromatograms of the data listed in the table by overlaying them.
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4.1 Hardware Validation Overview
Hardware validation is the process of verifying and documenting that the analytical instruments used for analysis function properly and can be used for the intended analysis.
Given that analytical instrument performance can vary over time, due to deterioration of consumables or components, the hardware must be periodically validated between the time it is introduced and disposed of. Compared to the various other types of validation related to analysis, such as analytical method validation and system suitability testing, hardware validation serves as a prerequisite for and is more important than the other types of validation.
In the CL model, it is recommended to have a hardware validation contract. This is a contract under which a qualified Shimadzu-approved engineer performs periodic component and system validation, and provides reports of the results. Details of the contract can be obtained from your Shimadzu representative.