Aktifitas antioksidan Spektrofotometer (1)

(1)

Metode Acuan :IK 18-9-97/MU

Paraf

Tanggal Pengujian : 28/04/25 Supervisor

Tanggal Pelaporan : 28/04/25

No. Instrumen : SIG/FNA/ALB/IN-0219

Ascorbic Acid (SIGMA-ALDRICH Cas No. 50-81-7 PHR1008-2G, Lot : LRAD1873) (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid( Sigma Cas No.53188-07-1 kat 238813-1G; BCCF0260)

M. Fajar

mg mg

25 mL 25 mL

99.99 % 99.2 %

0.00 mg/L 0.00 mg/L

Sampel

Blank MeOH + ddH2O 0.0000

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2535.3 1.0 25.0 101412.00 1.00 10 10141.20 0.9936 1.96 10559.74 3.96

Sampel_2 2535.3 1.0 25.0 101412.00 2.00 10 20282.40 0.9749 3.81 20983.16 3.34

Sampel_3 2535.3 1.0 25.0 101412.00 4.00 10 40564.80 0.9395 7.30 40715.20 0.37

Sampel_4 2535.3 1.0 25.0 101412.00 6.00 10 60847.20 0.9065 10.56 59109.47 -2.94

Sampel_5 2535.3 1.0 25.0 101412.00 8.00 10 81129.60 0.8652 14.63 82130.19 1.22

Slope 0.0000 0.0002 1.0

Intercept 0.0943

R²(Syarat: ≥ 0.995) 0.9990

R 0.9995

% Plot Residual -2,94 s/d 3,96

IC 50 281931.01 0.520

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2510.3 1.0 25.0 100412.00 1.00 10 10041.20 0.9919 2.13 11091.57 9.47

Sampel_2 2510.3 1.0 25.0 100412.00 2.00 10 20082.40 0.9748 3.82 20641.88 2.71

Sampel_3 2510.3 1.0 25.0 100412.00 4.00 10 40164.80 0.9394 7.31 40412.69 0.61

Sampel_4 2510.3 1.0 25.0 100412.00 6.00 10 60247.20 0.9074 10.47 58284.62 -3.37

Sampel_5 2510.3 1.0 25.0 100412.00 8.00 10 80329.60 0.8660 14.55 81406.42 1.32

Slope 0.0002

Intercept 0.1717

R²(Syarat: ≥ 0.995) 0.9985

R 0.9992

IC 50 282046.71

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2502.1 1.0 25.0 100084.00 1.00 10 10008.40 0.9870 2.61 9974.83 -0.34

Sampel_2 2502.1 1.0 25.0 100084.00 2.00 10 20016.80 0.9613 5.15 20219.90 1.00

Sampel_3 2502.1 1.0 25.0 100084.00 4.00 10 40033.60 0.9090 10.31 41068.81 2.52

Sampel_4 2502.1 1.0 25.0 100084.00 6.00 10 60050.40 0.8619 14.96 59844.79 -0.34

Sampel_5 2502.1 1.0 25.0 100084.00 8.00 10 80067.20 0.8122 19.86 79657.23 -0.51

Slope 0.0002

Intercept B9F100:J103 0.1458

R²(Syarat: ≥ 0.995) 0.9996

R 0.9998

IC 50 201422.04

504.R.303 Kefir

Volume Pelarutan

Sampel (mL) Kons. Sampel Induk

(mg/L) Volume Pemipetan

(mL) Volume Akhir

(mL) C. Deret sampel

(mg/L) Absorbansi

504.R.302_2 Kefir

No. Sampel Matriks Nama

Konsentrasi Sampel Induk Konsetrasi Deret Sampel

Bobot Porsi Uji (mg) Faktor Pengenceran

Sampel % Inhibisi Konsentrasi

Hitung % Plot Residual Volume Akhir

(mg/L) Absorbansi % Inhibisi Konsentrasi

Hitung % Plot Residual

Sampel Volume Pelarutan

(mg/L) Volume Pemipetan (mL)

% Inhibisi Konsentrasi

504.R.302 Kefir

Bobot Porsi Uji (mg) Faktor Pengenceran Sampel

Volume Pelarutan Sampel (mL)

Kons. Sampel Induk (mg/L)

Volume Pemipetan (mL)

Volume Akhir (mL)

C. Deret sampel (mg/L) Absorbansi :

C. Standar pembanding C. Standar pembanding : :

C. Standar Induk C. Standar Induk : :

Tanggal Pembuatan Deret Standar Tanggal Pembuatan Deret Standar :

:

Bobot Standar Induk Bobot Standar Induk : :

Volume Pelarutan Volume Pelarutan : :

Standar Induk Standar Induk : :

Tanggal Pembuatan Standar Induk Tanggal Pembuatan Standar Induk :

y = 0.0002x + 0.0943 R² = 0.999

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0002x + 0.1717 R² = 0.9985

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0002x + 0.1458 R² = 0.9996

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

(2)

Blank MeOH + ddH₂O 0.0000

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2500.7 1.0 25.0 100028.00 1.00 10 10002.80 0.9876 2.56 9826.64 -1.79

Sampel_2 2500.7 1.0 25.0 100028.00 2.00 10 20005.60 0.9616 5.12 20186.80 0.90

Sampel_3 2500.7 1.0 25.0 100028.00 4.00 10 40011.20 0.9089 10.32 41186.05 2.85

Sampel_4 2500.7 1.0 25.0 100028.00 6.00 10 60016.80 0.8624 14.91 59714.80 -0.51

Sampel_5 2500.7 1.0 25.0 100028.00 8.00 10 80022.40 0.8124 19.84 79638.19 -0.48

Slope 0.0002

Intercept 0.1222

R²(Syarat: ≥ 0.995) 0.9996

R 0.9998

IC 50 201429.88

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2519.2 1.0 25.0 100768.00 1.00 10 10076.80 0.9790 3.40 9811.45 -2.70

Sampel_2 2519.2 1.0 25.0 100768.00 2.00 10 20153.60 0.9416 7.09 20565.65 2.00

Sampel_3 2519.2 1.0 25.0 100768.00 4.00 10 40307.20 0.8727 13.89 40377.52 0.17

Sampel_4 2519.2 1.0 25.0 100768.00 6.00 10 60460.80 0.8029 20.78 60448.19 -0.02

Sampel_5 2519.2 1.0 25.0 100768.00 8.00 10 80614.40 0.7331 27.67 80518.85 -0.12

Slope 0.0000 0.0003

Intercept 0.0374

R²(Syarat: ≥ 0.995) 0.9999

R 1.0000

% Plot Residual -2,7 s/d 2

IC 50 145604.75

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2501.2 1.0 25.0 100048.00 1.00 10 10004.80 0.9788 3.42 9678.41 -3.37

Sampel_2 2501.2 1.0 25.0 100048.00 2.00 10 20009.60 0.9414 7.11 20449.27 2.15

Sampel_3 2501.2 1.0 25.0 100048.00 4.00 10 40019.20 0.8710 14.06 40723.82 1.73

Sampel_4 2501.2 1.0 25.0 100048.00 6.00 10 60028.80 0.8051 20.56 59702.41 -0.55

Sampel_5 2501.2 1.0 25.0 100048.00 8.00 10 80038.40 0.7351 27.47 79861.77 -0.22

Slope \ 0.0003

Intercept 0.1079

R²(Syarat: ≥ 0.995) 0.9998

R 0.9999

IC 50 145624.46

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2502.5 1.0 25.0 100100.00 1.00 10 10010.00 0.9782 3.48 10001.89 -0.08

Sampel_2 2502.5 1.0 25.0 100100.00 2.00 10 20020.00 0.9414 7.11 20635.42 2.98

Sampel_3 2502.5 1.0 25.0 100100.00 4.00 10 40040.00 0.8718 13.98 40746.67 1.73

Sampel_4 2502.5 1.0 25.0 100100.00 6.00 10 60060.00 0.8134 19.74 57621.63 -4.23

Sampel_5 2502.5 1.0 25.0 100100.00 8.00 10 80080.00 0.7311 27.86 81402.60 1.62

Slope 0.0003

Intercept 0.0677

R²(Syarat: ≥ 0.995) 0 0.9982

R 0.9991

146229.59

504.R.305 Kefir

Volume Akhir (mL)

C. Deret sampel (mg/L) Absorbansi

504.R.304_2 Kefir

(mL)

C. Deret sampel

504.R.304 Kefir

Faktor Pengenceran

(mL) Volume Akhir

(mg/L)

504.R.303_2 Kefir

Bobot Porsi Uji (mg) Absorbansi % Inhibisi Konsentrasi

(mL)

C. Deret sampel

y = 0.0002x + 0.1222 R² = 0.9996

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0003x + 0.0374 R² = 0.9999

0.00 5.00 10.00 15.00 20.00 25.00 30.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0003x + 0.1079 R² = 0.9998

0.00 5.00 10.00 15.00 20.00 25.00 30.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0003x + 0.0677 R² = 0.9982

0.00 5.00 10.00 15.00 20.00 25.00 30.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

(3)

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2513.0 1.0 25.0 100520.00 1.00 10 10052.00 0.9804 3.27 9534.53 -5.43

Sampel_2 2513.0 1.0 25.0 100520.00 2.00 10 20104.00 0.9414 7.11 20790.17 3.30

Sampel_3 2513.0 1.0 25.0 100520.00 4.00 10 40208.00 0.8690 14.26 41685.26 3.54

Sampel_4 2513.0 1.0 25.0 100520.00 6.00 10 60312.00 0.8153 19.56 57183.41 -5.47

Sampel_5 2513.0 1.0 25.0 100520.00 8.00 10 80416.00 0.7296 28.01 81916.95 1.83

Slope \ 0.0003

Intercept 0.0063

R²(Syarat: ≥ 0.995) 0.9969

R 0.9984

IC 50 146232.83

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2539.1 1.0 25.0 101564.00 1.00 10 10156.40 0.9916 2.16 10576.22 3.97

Sampel_2 2539.1 1.0 25.0 101564.00 2.00 10 20312.80 0.9709 4.20 19817.64 -2.50

Sampel_3 2539.1 1.0 25.0 101564.00 4.00 10 40625.60 0.9273 8.51 39282.66 -3.42

Sampel_4 2539.1 1.0 25.0 101564.00 6.00 10 60938.40 0.8799 13.18 60444.18 -0.82

Sampel_5 2539.1 1.0 25.0 101564.00 8.00 10 81251.20 0.8308 18.03 82364.65 1.35

Slope 0.0002

Intercept -0.1766

R²(Syarat: ≥ 0.995) 0.9991

R 0.9996

IC 50 227035.31

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2503.7 1.0 25.0 100148.00 1.00 10 10014.80 0.9907 2.25 10770.15 7.01

Sampel_2 2503.7 1.0 25.0 100148.00 2.00 10 20029.60 0.9714 4.15 19390.06 -3.30

Sampel_3 2503.7 1.0 25.0 100148.00 4.00 10 40059.20 0.9276 8.48 38952.35 -2.84

Sampel_4 2503.7 1.0 25.0 100148.00 6.00 10 60088.80 0.8822 12.96 59229.24 -1.45

Sampel_5 2503.7 1.0 25.0 100148.00 8.00 10 80118.40 0.8326 17.85 81381.96 1.55

Slope \ 0.0002

Intercept -0.1297

R²(Syarat: ≥ 0.995) 0.9990

R 0.9995

IC 50 226915.55

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2501.4 1.0 25.0 100056.00 1.00 10 10005.60 0.9817 3.14 11553.82 13.40

Sampel_2 2501.4 1.0 25.0 100056.00 2.00 10 20011.20 0.9607 5.21 19856.79 -0.78

Sampel_3 2501.4 1.0 25.0 100056.00 4.00 10 40022.40 0.9098 10.23 39981.61 -0.10

Sampel_4 2501.4 1.0 25.0 100056.00 6.00 10 60033.60 0.8611 15.04 59236.60 -1.35

Sampel_5 2501.4 1.0 25.0 100056.00 8.00 10 80044.80 0.8073 20.35 80508.02 0.58

Slope \ 0.0002

Intercept 0.2544

R²(Syarat: ≥ 0.995) 0.9991

R 0.9995

IC 50 199339.35

504.R.307 Kefir

Volume Pelarutan

(mL) Volume Akhir

(mg/L) Absorbansi

504.R.306_2 Kefir

504.R.306 Kefir

2539.8 Faktor Pengenceran

(mL) Volume Akhir

(mg/L)

504.R.305_2 Kefir

y = 0.0003x + 0.0063 R² = 0.9969

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

y = 0.0002x - 0.1766 R² = 0.9991

-5.00 0.00 5.00 10.00 15.00 20.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0002x - 0.1297 R² = 0.999

-2.00 0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0002x + 0.2544 R² = 0.9991

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

(4)

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2501.0 1.0 25.0 100040.00 1.00 10 10004.00 0.9801 3.30 12078.35 17.17

Sampel_2 2501.0 1.0 25.0 100040.00 2.00 10 20008.00 0.9610 5.18 19646.52 -1.84

Sampel_3 2501.0 1.0 25.0 100040.00 4.00 10 40016.00 0.9104 10.17 39696.23 -0.81

Sampel_4 2501.0 1.0 25.0 100040.00 6.00 10 60024.00 0.8614 15.01 59111.95 -1.54

Sampel_5 2501.0 1.0 25.0 100040.00 8.00 10 80032.00 0.8069 20.38 80707.00 0.84

Slope 0.0002

Intercept 0.2879

R²(Syarat: ≥ 0.995) 0.9985

R 0.9992

IC 50 199638.23

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2508.7 1.0 25.0 100348.00 1.00 10 10034.80 0.9849 2.82 10641.13 5.70

Sampel_2 2508.7 1.0 25.0 100348.00 2.00 10 20069.60 0.9610 5.18 20201.39 0.65

Sampel_3 2508.7 1.0 25.0 100348.00 4.00 10 40139.20 0.9118 10.03 39881.93 -0.65

Sampel_4 2508.7 1.0 25.0 100348.00 6.00 10 60208.80 0.8580 15.34 61402.52 1.94

Sampel_5 2508.7 1.0 25.0 100348.00 8.00 10 80278.40 0.8130 19.78 79403.01 -1.10

Slope 0.0002

Intercept 0.1971

R²(Syarat: ≥ 0.995) 0.9993

R 0.9997

IC 50 201906.35

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2520.2 1.0 25.0 100808.00 1.00 10 10080.80 0.9851 2.80 10668.57 5.51

Sampel_2 2520.2 1.0 25.0 100808.00 2.00 10 20161.60 0.9626 5.02 19664.03 -2.53

Sampel_3 2520.2 1.0 25.0 100808.00 4.00 10 40323.20 0.9091 10.30 41053.22 1.78

Sampel_4 2520.2 1.0 25.0 100808.00 6.00 10 60484.80 0.8587 15.27 61203.03 1.17

Sampel_5 2520.2 1.0 25.0 100808.00 8.00 10 80646.40 0.8122 19.86 79793.64 -1.07

Slope 0.0002

Intercept 0.1692

R²(Syarat: ≥ 0.995) 0.9994

R 0.9997

IC 50 201911.92

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2510.8 1.0 25.0 100432.00 1.00 10 10043.20 0.9843 2.88 10748.12 6.56

Sampel_2 2510.8 1.0 25.0 100432.00 2.00 10 20086.40 0.9607 5.21 20365.37 1.37

Sampel_3 2510.8 1.0 25.0 100432.00 4.00 10 40172.80 0.9107 10.14 40740.88 1.39

Sampel_4 2510.8 1.0 25.0 100432.00 6.00 10 60259.20 0.8624 14.91 60423.63 0.27

Sampel_5 2510.8 1.0 25.0 100432.00 8.00 10 80345.60 0.8149 19.60 79780.38 -0.71

Slope 0.0002

Intercept 0.2787

R²(Syarat: ≥ 0.995) 0.9995

R 0.9997

IC 50 205354.69

504.R.309 Kefir

Volume Pelarutan

(mL) Volume Akhir

(mg/L) Absorbansi

504.R.308_2 Kefir

504.R.308 Kefir

Volume Pelarutan

(mL) Volume Akhir

(mg/L) Absorbansi

504.R.307_2 Kefir

(mL)

C. Deret sampel

y = 0.0002x + 0.2879 R² = 0.9985

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0002x + 0.1971 R² = 0.9993

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0002x + 0.1692 R² = 0.9994

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0002x + 0.2787 R² = 0.9995

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

(5)

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2531.7 1.0 25.0 101268.00 1.00 10 10126.80 0.9843 2.88 10827.13 6.47

Sampel_2 2531.7 1.0 25.0 101268.00 2.00 10 20253.60 0.9604 5.24 20562.46 1.50

Sampel_3 2531.7 1.0 25.0 101268.00 4.00 10 40507.20 0.9104 10.17 40929.26 1.03

Sampel_4 2531.7 1.0 25.0 101268.00 6.00 10 60760.80 0.8616 14.99 60807.25 0.08

Sampel_5 2531.7 1.0 25.0 101268.00 8.00 10 81014.40 0.8130 19.78 80603.78 -0.51

Slope 0.0002

Intercept 0.2585

R²(Syarat: ≥ 0.995) 0.9996

R 0.9998

% Plot Residual

IC 50 205350.41

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2504.7 1.0 25.0 100188.00 1.00 10 10018.80 1.0001 1.32 9504.19 -5.41

Sampel_2 2504.7 1.0 25.0 100188.00 2.00 10 20037.60 0.9852 2.79 19910.42 -0.64

Sampel_3 2504.7 1.0 25.0 100188.00 4.00 10 40075.20 0.9548 5.79 41141.92 2.59

Sampel_4 2504.7 1.0 25.0 100188.00 6.00 10 60112.80 0.9284 8.40 59579.81 -0.89

Sampel_5 2504.7 1.0 25.0 100188.00 8.00 10 80150.40 0.8990 11.30 80112.91 -0.05

Slope 0.0001

Intercept -0.0206

R²(Syarat: ≥ 0.995) 0.9996

R 0.9998

IC 50 354062.17

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2512.1 1.0 25.0 100484.00 1.00 10 10048.40 1.0008 1.25 9367.88 -7.26

Sampel_2 2512.1 1.0 25.0 100484.00 2.00 10 20096.80 0.9861 2.70 19623.38 -2.41

Sampel_3 2512.1 1.0 25.0 100484.00 4.00 10 40193.60 0.9548 5.79 41459.90 3.05

Sampel_4 2512.1 1.0 25.0 100484.00 6.00 10 60290.40 0.9289 8.35 59529.10 -1.28

Sampel_5 2512.1 1.0 25.0 100484.00 8.00 10 80387.20 0.8988 11.32 80528.44 0.18

Slope 0.0001

Intercept -0.0718

R²(Syarat: ≥ 0.995) 0.9993

R 0.9997

% Plot Residual -7.26 s/d 3.05

IC 50 354043.13

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2516.9 1.0 25.0 100676.00 1.00 10 10067.60 0.9962 1.71 9286.36 -8.41

Sampel_2 2516.9 1.0 25.0 100676.00 2.00 10 20135.20 0.9755 3.75 20105.49 -0.15

Sampel_3 2516.9 1.0 25.0 100676.00 4.00 10 40270.40 0.9345 7.79 41534.70 3.04

Sampel_4 2516.9 1.0 25.0 100676.00 6.00 10 60405.60 0.8997 11.23 59723.39 -1.14

Sampel_5 2516.9 1.0 25.0 100676.00 8.00 10 80540.80 0.8599 15.16 80525.40 -0.02

Slope 0.0002

-0.0461

R²(Syarat: ≥ 0.995) 0.9994

R 0.9997

IC 50 265104.02

504.R.311 Kefir

Volume Akhir (mL)

C. Deret sampel (mg/L) Absorbansi

504.R.310_2 Kefir

504.R.310 Kefir

Volume Pelarutan

(mL) Volume Akhir

(mg/L) Absorbansi

504.R.309_2 Kefir

y = 0.0002x + 0.2585 R² = 0.9996

0.00 5.00 10.00 15.00 20.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

y = 0.0001x - 0.0206 R² = 0.9996

-2.00 0.00 2.00 4.00 6.00 8.00 10.00 12.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0001x - 0.0718 R² = 0.9993

-2.00 0.00 2.00 4.00 6.00 8.00 10.00 12.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0002x - 0.0461 R² = 0.9994

-2.00 0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

(6)

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2503.5 1.0 25.0 100140.00 1.00 10 10014.00 0.9936 1.96 10302.31 2.80

Sampel_2 2503.5 1.0 25.0 100140.00 2.00 10 20028.00 0.9761 3.69 19461.29 -2.91

Sampel_3 2503.5 1.0 25.0 100140.00 4.00 10 40056.00 0.9354 7.71 40762.46 1.73

Sampel_4 2503.5 1.0 25.0 100140.00 6.00 10 60084.00 0.8990 11.30 59813.13 -0.45

Sampel_5 2503.5 1.0 25.0 100140.00 8.00 10 80112.00 0.8603 15.12 80067.56 -0.06

Slope 0.0002

Intercept 0.0213

R²(Syarat: ≥ 0.995) 0.9998

R 0.9999

IC 50 265105.09

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2501.2 1.0 25.0 100048.00 1.00 10 10004.80 0.9848 2.83 10523.09 4.93

Sampel_2 2501.2 1.0 25.0 100048.00 2.00 10 20009.60 0.9613 5.15 19333.33 -3.50

Sampel_3 2501.2 1.0 25.0 100048.00 4.00 10 40019.20 0.9055 10.66 40252.96 0.58

Sampel_4 2501.2 1.0 25.0 100048.00 6.00 10 60028.80 0.8509 16.04 60722.70 1.14

Sampel_5 2501.2 1.0 25.0 100048.00 8.00 10 80038.40 0.8008 20.99 79505.38 -0.67

Slope 0.0003

Intercept 0.0623

R²(Syarat: ≥ 0.995) 0.9997

R 0.9998

IC 50 189745.83

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2503.8 1.0 25.0 100152.00 1.00 10 10015.20 0.9843 2.88 10658.64 6.04

Sampel_2 2503.8 1.0 25.0 100152.00 2.00 10 20030.40 0.9613 5.15 19283.93 -3.87

Sampel_3 2503.8 1.0 25.0 100152.00 4.00 10 40060.80 0.9047 10.74 40509.63 1.11

Sampel_4 2503.8 1.0 25.0 100152.00 6.00 10 60091.20 0.8518 15.95 60347.79 0.43

Sampel_5 2503.8 1.0 25.0 100152.00 8.00 10 80121.60 0.7999 21.08 79810.93 -0.39

Slope 0.0003

Intercept 0.0768

R²(Syarat: ≥ 0.995) 0.9997

R 0.9999

IC 50 189745.82

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2501.7 1.0 25.0 100068.00 1.00 10 10006.80 0.9952 1.81 9867.38 -1.41

Sampel_2 2501.7 1.0 25.0 100068.00 2.00 10 20013.60 0.9756 3.74 20535.95 2.54

Sampel_3 2501.7 1.0 25.0 100068.00 4.00 10 40027.20 0.9407 7.18 39532.54 -1.25

Sampel_4 2501.7 1.0 25.0 100068.00 6.00 10 60040.80 0.9025 10.95 60325.37 0.47

Sampel_5 2501.7 1.0 25.0 100068.00 8.00 10 80054.40 0.8664 14.51 79975.14 -0.10

Slope 0.0002

Intercept 0.0170

R²(Syarat: ≥ 0.995) 0.9999

R 0.9999

IC 50 275738.00

504.R.313 Kefir

Faktor Pengenceran

(mL) Volume Akhir

(mg/L)

504.R.312_2 Kefir

504.R.312 Kefir

Volume Pelarutan

(mL) Volume Akhir

(mg/L) Absorbansi

504.R.311_2 Kefir

(mL)

C. Deret sampel

y = 0.0002x + 0.0213 R² = 0.9998

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0003x + 0.0623 R² = 0.9997

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0003x + 0.0768 R² = 0.9997

0.00 5.00 10.00 15.00 20.00 25.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 0.0002x + 0.017 R² = 0.9999

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

(7)

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2501.1 1.0 25.0 100044.00 1.00 10 10004.40 0.9955 1.78 9735.14 -2.77

Sampel_2 2501.1 1.0 25.0 100044.00 2.00 10 20008.80 0.9754 3.76 20674.50 3.22

Sampel_3 2501.1 1.0 25.0 100044.00 4.00 10 40017.60 0.9407 7.18 39559.86 -1.16

Sampel_4 2501.1 1.0 25.0 100044.00 6.00 10 60026.40 0.9029 10.91 60132.38 0.18

Sampel_5 2501.1 1.0 25.0 100044.00 8.00 10 80035.20 0.8663 14.52 80051.81 0.02

Slope 0.0002

Intercept 0.0111

R²(Syarat: ≥ 0.995) 0.9998

R 0.9999

IC 50 275735.71

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2506.8 1.0 25.0 100272.00 1.00 10 10027.20 1.0008 1.25 10952.91 8.45

Sampel_2 2506.8 1.0 25.0 100272.00 2.00 10 20054.40 0.9901 2.31 21686.53 7.53

Sampel_3 2506.8 1.0 25.0 100272.00 4.00 10 40108.80 0.9716 4.13 40244.66 0.34

Sampel_4 2506.8 1.0 25.0 100272.00 6.00 10 60163.20 0.9530 5.97 58903.11 -2.14

Sampel_5 2506.8 1.0 25.0 100272.00 8.00 10 80217.60 0.9314 8.10 80570.99 0.44

Slope 0.0001

Intercept 0.1758

R²(Syarat: ≥ 0.995) 0.9982

R 0.9991

IC 50 506555.40

Blank DPPH 100 0.00 1.0135 0.00

Sampel_1 2516.5 1.0 25.0 100660.00 1.00 10 10066.00 1.0003 1.30 11105.08 9.36

Sampel_2 2516.5 1.0 25.0 100660.00 2.00 10 20132.00 0.9900 2.32 21444.76 6.12

Sampel_3 2516.5 1.0 25.0 100660.00 4.00 10 40264.00 0.9702 4.27 41321.04 2.56

Sampel_4 2516.5 1.0 25.0 100660.00 6.00 10 60396.00 0.9523 6.04 59290.00 -1.87

Sampel_5 2516.5 1.0 25.0 100660.00 8.00 10 80528.00 0.9313 8.11 80370.91 -0.20

Slope 0.0001

Intercept 0.2109

R²(Syarat: ≥ 0.995) 0.9980

R 0.9990

IC 50 506556.53

Perhitungan:

Konsentrasi Sampel Induk(mg/L) = (Bobot Porsi Uji (mg) / (Faktor Pengenceran x Volume Pelarutan)) x 1000 C. Deret sampel (mg/L) = (Konsentrasi sampel x Volume Pemipetan) / Volume Akhir (Volume labu) Konsentrasi hitung = (% Inhibisi - intercept) / slope

% Residual = (Konsentrasi hitung - C.Deret) / Konsentrasi hitung x 100

504.R.314_2 Kefir

Volume Akhir

504.R.314 Kefir

Volume Pelarutan

(mL) Volume Akhir

(mg/L) Absorbansi

504.R.313_2 Kefir

IC =50 − 𝑖𝑛𝑡𝑒𝑟𝑐𝑒𝑝𝑡 𝑠𝑙𝑜𝑝𝑒

y = 0.0002x + 0.0111 R² = 0.9998

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

y = 1E-04x + 0.1758 R² = 0.9982

0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)

Kurva Sampel

y = 1E-04x + 0.2109 R² = 0.998

0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00

0.00 20000.00 40000.00 60000.00 80000.00 100000.00

% Inhibisi

Konsentrasi (mg/L)