POLICY STATEMENT
RESPONSIBILITIES
Assist the faculty in submitting registrations to the Institutional Biosafety Committee and maintain registration files. Comply with this Manual, the University Chemical Hygiene Plan, the University Radiation Safety Manual, and all applicable University OPs relating to safety and health;. Reviews rDNA research conducted at or sponsored by the University for compliance with the NIH guidelines, and approves those research projects found to meet the NIH guidelines;.
Review research involving infectious agents at or sponsored by the University for compliance with the guidelines in Biosafety in Microbiological and Biomedical Laboratories (BMBL), and approve those research projects found to comply with the. Report any significant problems with or violations of NIH guidelines and any significant research-related accidents or illness to the appropriate institutional official and to the NIH Office of Recombinant DNA Activities (ORDA) within 30 days; and. Follow the guidelines for membership defined by NIH, with the additional requirement of one representative from the Indiana University-Purdue University Indianapolis Institutional Animal Care and Use Committee.
EMERGENCY PROCEDURES
Laboratories and animal care areas must be separated from the public areas of the buildings in which they are located. All packages must be screened (visually and/or radiologically) before being taken to the laboratory. Packages containing specimens, bacterial or viral isolates, or toxins should be opened in a safety cabinet or other suitable containment device.
If you transport biological materials or toxins by hand in passenger vehicles or on conventional carriers, all applicable regulations must be followed. Chemicals and radioactive materials must be disposed of in accordance with local, state, and federal regulations. Department heads, laboratory directors, principal investigators, laboratory workers, the Office of Environmental Health and Safety, and IUPUI Public Safety must be involved in emergency planning.
Safety incident reports should be sent to IUPUI Public Safety and the Environmental Health and Safety Office.
DECONTAMINATION AND DISPOSAL
The concentration used is dependent on the organic load of the material to be decontaminated. Before using the autoclave, check the drain screen at the bottom of the chamber and clean it if it is blocked. Both types of tests should be placed well down in the center of the bag or container of waste, at the point that heats up the slowest.
First, the chemical test must be performed to determine that the temperature in the center of the container reaches 121°C. Sharps contaminated with radioactive material should be disposed of in separate sharps containers labeled with the name of the isotope. Biowaste boxes (incinerators) can be purchased from various sources, for example from laboratory products distributors.
To prevent contamination of the box, a plastic cover should be used, also provided by the seller.
CLASSIFICATION OF POTENTIALLY INFECTIOUS AGENTS
Animal biosafety level 2 procedures, containment equipment and facilities are recommended for studies using experimentally infected laboratory rodents. For all manipulations with cultures of pathogenic Brucella spp. biosecurity level 3 and animal biosecurity level 3 practices, containment equipment and facilities are recommended. Recommended Precautions: Biosafety Level 2 practices, containment equipment, and facilities are recommended when handling cultures or potentially infectious clinical materials.
Biosafety Level 2 practices, containment equipment and facilities are recommended for activities involving naturally or experimentally infected animals. Laboratory Hazards: Accidental parenteral inoculation and ingestion of the toxin are the primary hazards to laboratory personnel. Recommended Precautions: Biosafety Level 2 practices, containment equipment and facilities are recommended for activities involving manipulation of cultures or toxin.
Recommended Precautions: Biosafety Level 2 practices, containment equipment, and facilities are recommended for all activities using known or potentially infectious clinical materials or cultures. Animal biosecurity level 2 practices, containment equipment and facilities are recommended for operations with experimentally or naturally infected animals. Recommended precautions: When working with clinical materials and cultures of Mycobacterium spp. biosafety level 2 practices, containment equipment and facilities are recommended.
For animal studies with mycobacteria other than M. Recommended Precautions: Biosafety Level 2 practices, containment equipment, and facilities are recommended for all activities using known or potentially infectious clinical materials and cultures. Recommended Precautions: Biosafety Level 2 practices and facilities are recommended for activities with infective levels of the listed parasites.
Recommended Precautions: Biosafety Level 2 practices and facilities are recommended for working with infective stages of these parasites. Recommended Precautions: Biosafety Level 2 practices and equipment are recommended for activities with listed infectious stages. Laboratory Hazards: The agent may be present in the blood, cerebrospinal fluid, urine, nasopharyngeal secretions, feces, and tissues of infected animal hosts and possibly humans.
Recommended Precautions: Biosafety Level 2 practices and facilities are recommended for all activities involving known or potentially infectious materials.
RISK ASSESSMENT
Access to the laboratory is restricted or limited at the discretion of the laboratory director when experiments or work with cultures and specimens are in progress. A biohazard sign can be posted at the entrance to the laboratory when infectious agents are present. Access to the laboratory is restricted or limited at the discretion of the laboratory director when experiments are in progress.
Access to the laboratory is limited or limited by the laboratory director when working. A biohazard sign must be posted at the entrance to the laboratory when etiologic agents are used. Spills and accidents resulting in overt exposure to infectious material are reported immediately to the laboratory director.
Eating, drinking, smoking, wearing contact lenses and applying cosmetics are not allowed in the laboratory. Additional serum samples may be collected periodically, depending on the agents treated or the function of the laboratory. Animals and plants not related to the work being carried out are not allowed in the laboratory.
Consideration should be given to installing an HVAC control system to prevent sustained positive pressure in the laboratory. The laboratory director is specifically and primarily responsible for the safe operation of the laboratory. Access to the laboratory is restricted by the laboratory director when experiments are in progress.
Laboratory personnel receive available immunizations for the drugs handed over or potentially present in the laboratory. The air flow in the supply and exhaust components is monitored and the HVAC control system is designed to prevent sustained positive pressure of the laboratory.
BIOSAFETY EQUIPMENT
BSC
Certification in countries other than the final country can attest to the performance capability of. As with any other laboratory equipment, personnel must be trained in the proper use of biological safety cabinets. All operations in the work area of the cabinet are carried out via arm-length or half-suit attached rubber gloves.
All equipment required by the laboratory activity, such as incubators, refrigerators and centrifuges, should be an integral part of the cabinet system. Work as much as possible at the back (beyond the air gap) of the BSC workspace. If a flame is needed, use a burner with a pilot light and place it at the back of the work space.
UV radiation should not replace 70% ethanol for disinfecting the interior of the cabinet. Application for authorization must be submitted at least 10 working days before the expected date of dispatch. The application must be submitted at least 120 days before the expected release or shipment date.
HEPA and/or carbon filtration of the exhaust air may be required, depending on the toxin. The user should check the inward airflow of the hood or biological safety cabinet before starting work. All work must be performed within the operationally effective zone of the fume hood or biological safety cabinet.
Other protective equipment may be required depending on the characteristics of the toxin and containment system. Including an IPM policy statement in the facility's standard operating procedures can increase awareness of the program.
BSC
BSC
SHIPMENT OF BIOLOGICAL MATERIALS
The import authorization, with proper packaging and labeling, will expedite clearance of the infectious materials package through the U.S. facility. Registration and completion of CDC Form EA-101 are required by the CDC prior to transfer of selected infectious agents and toxins (42 CFR part 72). Contact the Biological Safety Officer if your work contains any of the agents listed in Appendix C.
Biological transfer regulations are intended to ensure that the change of ownership of biological materials is in the public and national interest. The reader is also advised to refer to the Department of Transportation's additional hazardous materials provisions (49 CFR Parts 171-180). Importation, possession, or use of the following items is prohibited or restricted by law or the United States.
The importation, possession, use, or interstate shipment of animal pathogens other than those listed above may also be subject to US regulations. The material below is adapted from the Biological Defense Safety Program, Technical Safety Requirements (DA Pamphlet 385-69)1 and Appendix A of the Occupational Safety and Health Association Rule of the United States Department of Occupational Exposure to dangerous chemicals in laboratories"2. Before containers are removed from the lid, cabinet, or glove box, the exterior of the closed primary container must be decontaminated and placed in a clean secondary container.
The inside of the hood, glove box, or cabinet should be decontaminated periodically, for example, at the end of a series of related experiments. Genetically modified microorganisms or genetic elements containing nucleic acid sequences encoding any of the toxins listed in this Annex, or their toxic units. The decision to implement an IPM program should be based not only on the cost of services, but also on the effectiveness of the program components.
Training on topics such as pest identification, biology, and sanitation can also promote understanding and collaboration with the IPM program objectives. This is to ensure that the program controls pests and meets the specific needs of the facility program(s) and its residents.
