The Formulation of Peel-Off Mask Gel of Mangosteen Rind Extract (Garcinia mangostana L.) with Ethanol 50% and Evaluation of Its Antioxidant activity

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The Formulation of Peel-Off Mask Gel of Mangosteen Rind Extract (Garcinia mangostana L.) with Ethanol 50% and Evaluation of Its Antioxidant activity

Nelly Suryani, OfaSuzanti Betha, Myra Kharisma Izzati

Pharmacy Departemen Faculty of Medicine and Health Science UIN SyarifHidayatullah Jakarta

ABSTRACT

Mangosteen (Garcinia mangostana L.) is a plant that rich in antioxidant compounds. Various products containing antioxidant compounds have been widely used, such as gel peel-off mask. This study aimed to obtain a gel peel-off mask formulation of mangosteen rind extract (Garcinia mangostana L.) with a base of polyvinyl alcohol (PVA) and Hidroxy Prophyl Methyl Cellulosa (HPMC) as a viscosity enhancher, as well as to determine the activity of antioxidant of peel-off mask gel mangosteen rind extract (Garcinia mangostana L.). Antioxidant activity was tested by using DPPH (1,1-difenil-2-pikrilhidrazil). Mangosteen rind was extracted by maceration using ethanol 50%. The extract was made to be gel peel-off mask with mangosteen rind extract 1% and viscosity enhancer variation of HPMC 1% (F1), 2% (F2), 3% (F3) and gel without extract with HPMC 3% as control (F4). The results showed that increase in concentration of HPMC in the formula affect the greater viscosity, decrease in gel spreadness, decrease in tensile strength and elongationthat was significantly different (p˂ 0,05).

Antioxidants determination using DPPH assay showed that the stability of antioxidant of the peel-off mask gel affects the activity of antioxidant peel-off gel.

Keywords: peel-off mask gel, PVA, HPMC, mangosteen rind extract.

Curcuma zedoaria (Christm.) Roscoe.)

(Private collection)

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Methods

The powder from tuber of Curcuma zedoaria (Christm.) Roscoe.) and herb of (Andrographis paniculata Ness)

Extracted with ethanol 96% ( 1: 4) for 24 hours, liquid extract was filtrated and evaporated 50^oC

Crude extract tuber of Curcuma zedoaria (Christm.) Roscoe. and herb of

(Andrographis paniculata Ness (TS)

Crude Extract was irradiated by Gamma radiation 10 kGy.

Crude Extract after irradiation and no irradiation was tested for antibacterial activity to Bacillus subtilis ATCC 6633 dan

Staphylococcusaureus ATCC 25923

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Phytochemical Screening results.

Extracts Caracterization

Curcuma zedoaria (Christm.) Roscoe.)

Andrographis paniculata Nees) 0 kGy 10 kGy 0 kGy 10 kGy Parameter spesifik:

a. Identity Crude extract Crude extract

b. Organoleptic

 Consistency

 Colour

 Odor

Thick Brown Aromatic

Thick Green Aromatic

c. Solubility

 Water

 Ethanol

37,5%

47%

26,5%

62,5%

43,5%

61,5%

43,5%

56,5%

a. Ash content 1,73% 1,76% 0,56% 0,57%

Phytochemistry Screening

Alcaloids + + + +

Flavonoids + + + +

Tanin - - + +

Saponin - - - -

Terpenoid + + + +

Phenol - - + +

*)

+= positive reaction.

- = negative reaction

.

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Explanation :

There was fenomena that the higher concentration of TS irradiation and TS non irradiation caused the increased of % inhibition to the growth of B. subtilis.

50 55 60 65 70 75 80 85 90 95 100

62,5 125 250 500 1000

% Inhibition

Concentration (µg/mL) Relationship of concentration TS and inhibition % TS non irradiation and

irradiation to growth of Bacillus subtilis and Staphylococcus aureus

TS 0 kGy-B.subtilis TS 10 kGy-B.subtilis TS 0 kGy-S. aureus TS 10 kGy-S. aureus

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Inhibition zone of TS irradiation and TS non irradiation togrowth of Bacillus subtilis

Inhibition zone of

TS irradiation and TS non irradiation

to growth of Staphylococcus aureus

.

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Tabel 3.

Inhibition zone (mm) extract Curcuma zedoaria (Christ) (ECZ), Andrographispaniculata (Ness) (EPC), and combination extract Curcuma zedoaria (Christ) and

Andrographispaniculata (Ness) (TS)to growth of Bacillus subtilis andStaphylococcus aureus

Inhibiton zone diameter (mm)

Sampel Concentration B. subtilis S. aureus

ECZ 0 kGy

10 µg - -

100 µg 11,75 12

1000µg 12,5 12,5

ECZ 10 kGy

10 µg - -

100 µg 10 9,25

1000µg 11,5 10

EPC 0 kGy

10 µg - -

100 µg 10,75 10,25

1000µg 11,5 11

EPC 10 kGy

10 µg - -

100 µg 9,5 9,25

1000µg 10,5 9,5

TS 0 kGy

10 µg - -

100 µg 10,25 10,25

1000µg 10,5 11,5

TS 10 kGy

10 µg - -

100 µg 9 9,25

1000µg 9,75 10

Kanamycin 30 µg 18,00 14,00

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Inhibition Zone diameter (mm)of ECZ, EPC, TS non irradiation to growth ofBacillus subtilis

Inhibition Zone diameter (mm) of ECZ, EPC, TS non irradiation to growth ofStaphylococcus aureus

Relationship of inhibition zone to concentration of ECZ

Relationship of inhibition zone to concentration of EPC

Relationship of inhibition zone to concentration of TS

Relationship of inhibition zone to concentration of ECZ

Relationship of inhibition zone to

concentration of EPC Relationship of inhibition zone to concentration of TS

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Minimum Inhibition Concentration (% )

Sample concentration B. subtilis S. aureus

TS 0 kGy

62,5µg/mL 88,15 75,81

125µg/mL 89,63 79,14

250µg/mL 90,52 87,63

500µg/mL 92,59 91,08

1000µg/mL 95,11 96,34

TS 10 kGy

62,5µg/mL 84,15 68,39

125µg/mL 85,63 76,67

250µg/mL 88,44 81,51

500µg/mL 89,93 87,96

1000µg/mL 91,85 93,76

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Conclusion

Gamma Irradiation influenced the antibacterial activity of combination extract Curcumazedoaria (Christ) and Andrographispaniculata (Ness), (p ≤ 0,05) to growth Bacillus subtilisdanStaphylococcus aureusby using difusion method and dilution method

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