Supplemental Digital Content 5: Impact of trimester of COVID-19 infection on antibody levels in maternal blood, infant blood, and breast milk at 6-weeks
Specimen Type
Antibod y
First / Second Trimester Mean (SD)+
N= 20
Third Trimester Mean (SD)+
N= 10
Mean difference (95%
CI)*
P- valu e Spike Protein
Maternal blood
IgG 56177.3
(148612)
117021 (180808)
60843.3 (-78737.6, 200424)
0.38 IgM 3549.4 (5149.0) 1101.4 (1863.0) -2447.9 (-5601.2,
705.3)
0.12 IgA 3554.3 (7044.8) 2905.5 (3917.8) -648.8 (-5772.6,
4474.9)
0.80 Infant / cord
blood IgG 2337.4 (2740.5) 6714.3 (5238.6) 4376.9 (130.3, 8623.5) 0.04 IgM 25.4 (43.3) 25.3 (43.3) -0.2 (-45.1, 44.7) 0.99 IgA 42.1 (86.6) 702.0 (1681.5) 660.0 (-746.2, 2066.1) 0.30 Breast milk IgG 369.8 (1195.6) 5282.3
(16385.1)
4912.5 (-6819.0, 16644.0)
0.37 IgM 242.6 (674.6) 24.1 (16.8) -218.5 (701.2, 264.1) 0.33 IgA 2783.1 (2979.9) 917.5 (1095.7) -1865.6 (-3849.5,
118.3)
0.06 Nucleocapsid Protein
Maternal blood
IgG 10654.2
(27112.3)
6029.5 (14582.9)
-4624.7 (-24230.9, 14981.5)
0.63
IgM 10654.2
(27112.3)
6029.5 (14582.9)
-4624.7 (-24230.9, 14981.5)
0.63
IgA 40060.2
(137620)
6180.5 (6320.1) -33879.7 (-113409, 45649.5)
0.37 Infant / cord
blood
IgG 43580.5
(52482.6)
617503 (1308742)
573922 (-520362, 1668207)
0.26 IgM 99.2 (91.5) 118.6 (81.5) 19.4 (-70.7, 109.4) 0.65 IgA 35.4 (27.9) 293.1 (551.9) 257.7 (-203.8, 719.2) 0.23 Breast milk IgG 90674.5
(137204) 71940.2
(129051) -18734.2 (-138057,
100588) 0.75
IgM 70.2795 (104.9) 290.6 (344.8) 220.3 (-70.7365, 511.4) 0.12 IgA 6399.3 (6903.1) 2130.5 (2140.1) -4268.7 (-8799.4,
261.9)
0.06
*First/second trimester used as reference +All values reported as AU
