IPCB 2016

This extensive area provides abundant natural resources with a great variety of biodiversity that will enable the development of biotechnology in the future. The use of biotechnology has grown rapidly in the world for several reasons, especially because it is recognized as an environmentally friendly technology.

KEYNOTE SPEECH

Creation of a Recycling Society

Novel Lake Ecosystem Management by Sustainable Utilization of Aquatic Weed Biomass-

Challenges in Developing Marine Bio-products

The products that will be highlighted are Scalogel from fish scales, adjuvant from the microalgae and HEME-1, a marine additive produced to cultivate the marine bacteria from the oceans. Lessons from the past and charting the future for marine natural products, drug discovery and chemical biology.

Sarwoko Mangkoedihardjo

Phytotechnology for Bioremediation of Contaminated Soils – The Essential Conditions for Mixed Plants

Similar results were shown in applications of chelates, such as EDTA and EDGA, that were also able to improve the purification of soil contaminated with oil [9]. It was thus necessary to plan soil saturation and soil aeration, which were intended to be able to reduce various types of pollutants.

Biotechnology for Renewable Energy Production from Waste Treatment

By investigating 3 full-scale wastewater treatment plants, we demonstrate that the UASB sludge under methanogenic conditions will be favored over aerobic conditions for TMAH treatment due to its superior ability to handle high strength TMAH-containing wastewater. Furthermore, batch experiments were performed to evaluate mcrA mRNA expression during methanogenic TMAH degradation and the results indicated that a higher level of TMAH favored mcrA mRNA expression by Methansarcina, while Methanomethylovorane only increased a significant amount of mcrA mRNA at a lower level of TMAH could express.

Willy Verstraete

Developments in the Moving World of Environmental Biotechnology

Recently, in the field of resource recovery, a lot of novelty has been introduced in the field of bioelectrochemistry, which allows the production of products at the site of the cathode, using microbial biosynthesis, powered by recovered and renewable energy respectively. To produce recovered water and new proteins from recovered nitrogen, it is therefore necessary that the entire society is interested in the products thus generated.

Raul Muñoz

Innovative Wastewater Treatment by Algae and Bacterial Systems

1) Pusat Studi Lingkungan Hidup (PSLH), Institut Teknologi Bandung, Jl. Sangkuriang 42 A, Bandung 40135, Indonesia, Email: . tjandra@che.itb.ac.id.

Production of Biodegradable Polymers

Polyhydroxyalkanoate - PHA) through Biological Processes: Challenges and Opportunities

Development of a Bio-PORec system for the production of polyhydroxyalkanoates (PHA) and their storage in mixed cultures of palm oil mill effluents (POME), Bioresource Technology. Influence of aerobic and anoxic microenvironments on polyhydroxyalkanoate (PHA) production from food waste and acidogenic sewage using aerobic consortia, Bioresource Technology.

PLENARY LECTURES

Strain Development by using Genome Editing Technology to Improve Algal Mass Cultivation

Furthermore, the resulting transformants would be exempt from GMO regulation, facilitating the application of microalgae in the production of pharmaceuticals, nutraceuticals, food and animal feed. Our recent achievement in applying this genome editing method to improve mass cultivation for pigment production will make the use of microalgae more attractive and lucrative.

Edwan Kardena

Remediation of Contaminated Soil in Indonesia

Zooplankton Ecology Studies in the Strait of Malacca

Ozone-based Advanced Oxidation Process (AOP) techniques are currently developed using heterogeneous catalysts. This study treated leachate from an active landfill with O3/H2O2 with the addition of natural zeolite as a catalyst.

IPCB 2016

PREFACE

OPENING REMARKS

The latest and new biotechnological processes, combined with physical and/or chemical processes, have been carried out not only for the improvement of the environment, but also for the production of renewable energy and the recovery of resources, as added values, which become more important in the next century. Therefore, I strongly believe that this will strengthen our ties in the near future, not only in Asia, but can also create a new intercontinental bond.

PRESIDENT SOKA UNIVERSITY

Through this conference on the application of biotechnology in the management of scientific research, findings from research conducted from different parts of the world can be shared. The Organizing Committee is very honored with the participation of the President of SU, the Vice Chancellor of UMT, the Rector of ITS and the President of the Watanabe Oyster Laboratory in this conference.

KEYNOTE ADDRESS

The Current Development of Biotechnology in Indonesia

Innovation, Technological Progress and Economic Growth

Regional Cooperation in Biotechnology Research for Environmental Sustainability

Water saturation of the soil to produce anaerobic conditions may not apply to all pollutants present in the soil. A preliminary study on the distribution of the cyclopoid cephalopods of the family Oithonidae in Malaysian waters.

Table 1. Population groups of copepod genera and their occurrence in the waters off Port Dickson

Mitsugu Watanabe

A study was conducted to investigate the permeability of E6 in the brain after oral administration of E6 in mice. The MRM chromatogram of LC-MS/MS showed that the single peak of E6 was detected in the brain (Fig. 4).

Fig. 2. Chemical structure of 3,5-dihydroxy-4-metoxybenzyl alcohol (E6)

Applications of Biotechnology in Color Removal

1Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Environmental Sustainability, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Maleisië.

PROCEEDING

AQUATIC BIOTECHNOLOGY

Biosorption of Cadmium Ion from Aqueous Solution by Aphanothece sp: Equilibrium, Kinetic

INTRODUCTION

This technology is receiving considerable attention as it has been proven to be an efficient, clean and inexpensive way to treat wastewater containing heavy metals [6, 7]. Many studies in which microorganisms have been used as biosorbents for heavy metals are useful for the development of a low-cost industrial wastewater treatment.

MATERIAL and METHODS 1. Preparation of biomass

The thermodynamic behavior of Cd(II) biosorption onto dried biomass of Aphanothece sp was described using three thermodynamic parameters including free energy (ΔG0), enthalpy (ΔH0) and entropy (ΔS0). The equilibrium Cd biosorption capacity of dried biomass of Aphanothece sp at different initial Cd concentrations is shown in Figure 2.

Figure 1. Typical images of Aphanothece sp: (a) obtained with optical microscope (400x) and (b) scanning electron image microscope of dried biosorbent made of Aphanothece sp

CONCLUSIONS

Bhatia, “Biosorption of cadmium (II) ions by immobilized Pycnoporus sanguineus cells from aqueous solution,” Bioresource Technology , vol. Tuzen, “Biosorption of cadmium (II) from aqueous solution by the red alga Ceramium virgatum: Equilibrium, kinetic and thermodynamic studies,” Journal of Hazardous Materials , vol.

Settlements at Riverbanks

Case Study: Kejawan Gebang Region of Gebang Putih Surabaya)

PLANNING METHODS

Determining domestic wastewater discharge based on water consumption per person per day. While the quality of domestic sewage is derived from laboratory analysis of domestic sewage samples of Kejawan Gebang.

RESULTS AND DISCUSSION 1. Planning Overview

While the BOQ and construction cost calculation in this plan is carried out by calculating the dimensions of the domestic sewage treatment plant and the unit price cost of workers (HSPK) in Surabaya in 2015. The quality of domestic sewage from the results of laboratory analyzes are presented in Table 1.

Table 1. Domestic Waste water Quality of Kejawan Gebang No. Parameter Value (mg/L)

CONCLUSION

34; Effect of plant species on the water balance in a pilot-scale horizontal subsurface flow constructed wetland planted with Arunda donax L. Konnerup, Brix, Hans; Koottatep, T, "Treatment of domestic wastewater in tropical, subsurface flows of constructed wetlands covered with Canna and Heliconia," Elsevier.

Effect of different hydrolysis time on the properties of freeze dried protein hydrolysate from

Yellowstripe Scad (Selaroides leptolepis)

MATERIALS AND METHOD 1 Protein extraction

• Yield of powdered hydrolysate

Then, 75 ml of distilled water was carefully added to the cold tube and the distillation process continued. NaOH was poured into the tube and the distillation process was operated for 4 minutes until a light green solution was formed.

RESULTS AND DISCUSSION 1 Yield

The extent of hydrolysis also depends on the availability of sensitive peptide bonds on which the primary attack is based and the physical structure of the protein molecule (Kanu et al, 2009). The study clearly shows that 2 hours of hydrolysis is more preferable as it has the highest degree of hydrolysis (69%), which provides better functional properties of the protein hydrolyzate for further analysis.

Figure 1: Percentage yield obtained from hydrolysis

MARINE AND FRESHWATER ECOLOGY

Hydrophytes Bioaccumulation of Heavy Metals in Brantas Kedurus Surabaya, East Java

METHODS

RESULTS AND DISCUSSIONS

According to the result, cuprum collected 0.02 ppm and 0.03 ppm by eceng gondok and kangkung air. According to Figure 2 and Table 2, it can be seen that eceng gondok and kangkung air accumulated cuprum was 0.02 ppm and 0.61 ppm.

Figure 1. The result of heavy metals analyze in water hyacinth and water spinach organs

CONCLUSSION

According to the statistical analysis, there was a difference, because the organ variable P was 0.004. It showed that the stem was good copper accumulator, because the organ variable with P value was 0.01, it meant that H0 was accepted and H1 was rejected.

The New Deviation Morphology of Hydrodictyon sp

MATERIALS AND METHOD

This alga was different with [3] stating that Hydrodictyon was formed from hexagonal meshes in the web. We also found a bud that had two branches at the angle of the alga (Figure 6).

Table 1. The result of abiotic factors measurement

NATURAL RESOURCES AND NATURAL PRODUCTS

Potential of Yeast Isolated from Kerdas Seed (Archidendron Bubalinum) as a Leavening Agent in

Bread Making

MATERIAL AND METHOD 1 Material

The collected samples were placed aseptically in sterile plastic bags, transferred to ice boxes (4°C) and brought to the laboratory for analysis. Representative colonies were randomly picked from the plates and pure cultures were subjected to the next identification procedures.

RESULTS AND DISCUSSION

The control bread showed no significant difference (p>0.05) with the bread of yeast strains KB2, KB4 and KM8. Isolation of Saccharomyces cerevisiae strains producing higher levels of aromatic compounds for the production of "cachaca", a Brazilian sugarcane spirit.

Figure 1. Kerdas fruit used in the study (a) Premature kerdas, (b) mature kerdas and (c) over-mature kerdas

Evaluation of Tendering Effect from Date Seed extract (P. dactalytera) in Knuckle Part Meat

MATERIALS AND METHODS 1 Plant Material

It uses the senses to assess the overall acceptability and quality attributes of products. Based on the score and hedonic test, attributes such as juiciness, texture, taste and overall acceptance were examined. For acidity, litmus paper was used to determine the pH level of the sample before and after the cooking treatment.

Table 1. Evaluated Parametre

RESULT AND DISCUSSION 1 Extraction Yield

The water-holding capacity of fresh meat (the ability to retain natural water) is an important property of fresh meat as it affects both the yield and the quality of the final product. Completion of the cooking process is generally indicated by a color change from red to brown (red to pink in cured products) and flavors develop.

Table 5. Scoring Test

Identification of Bioactive Compounds of Thuidium tamariscellum

MATERIALS AND METHODS 1. Plant material

Peak Retention Time Compound Name Area (%). Wurtman et al., 2000), which is likely the compound responsible for the metabolic effects of cytidine. However, further research is aimed at testing the biological activity of each n-hexane, ethyl acetate and methanol extract of Thuidium tamariscellum.

Figure 1. Chromatogram profile of n-hexane extract of Thuidium tamariscellum

Bioprospecting of Fungi Associated with Cladiella sp. as Antibacterial-MDR against Acinetobacter

MATERIALS AND METHODS

The sample was transferred to a Petri dish already containing MEA (Malt Extract Agar) with seawater. The MEA that grew the fungus was then cut into cylindrical shapes to be placed on NA (Nutrient Agar) MDR-AB inoculated bacteria 1x24h at a density of 0.5 McFarland.

RESULTS

8 isolates were obtained from the isolation of fungi associated with Cladiella sp., with only one isolate active against MDR-AB (Table 1) encoded by FSP-11-A3. The ability of MDR-AB to produce biofilms [8] can prevent bioactive compounds from being secreted by fungi.

Table 1. Antibacterial Activity from Fungi Associated Cladiella sp.

CONCLUSIONS

One of the potential sources to search for natural products from the marine environment is soft corals. Therefore, fungi associated with soft corals can be a sustainable source due to the ability to grow quickly and environmentally friendly.

RENEWABLE ENERGY

Biogas Production and Biodegradability on Increasing Total Solid of Household Organic Waste

The anaerobic digestion of municipal organic waste was investigated with batch experiments at solids contents of 5%, 10% and 15%. The adaptation of increasing TS contents to the anaerobic digestion of municipal organic waste was achieved over a period of 13 – 20 days.

Table 1. Composition of Synthetic Food Waste [9]

Leachate Treatment using Advanced Oxidation Process with Zeolite as a Catalyst

RESULT

Turbidity removal describing the ability of ozone and OH· to remove both organic and inorganic from the leachate. This condition will improve the efficiency of the process to remove organic and inorganic material on the leachate.

Figure 3. pH Changes on Each Variation

Performances and Emissions Characteristics of Three Main Types Composition of Gasoline–

Ethanol Blended in Spark Ignition Engines

• COMPARISON OF GASOLINE-ETHANOL PROPERTIES AND ITS EFFECT ON COMBUSTIONS PROCESS
• COMBUSTION CHARACTERISTICS OF GASOLINE-ETHANOL BLENDS IN LOW COMPOSITION
• COMBUSTION CHARACTERISTICS OF GASOLINE-ETHANOL BLENDS IN MEDIUM COMPOSITION
• COMBUSTION CHARACTERISTICS OF GASOLINE-ETHANOL BLENDS IN HIGH COMPOSITION
• CONCLUSION

The phenomenon can be overcome by adjusting the compression ratio of the engine according to the concentration of ethanol in the gasoline. Therefore, adjusting the compression ratio will decrease the BSFC value with increasing ethanol concentrations.

Table 1. Comparisons of Properties of Gasoline and Ethanol (5)

RESTORATION ECOLOGY

Utilization of Water Hyacinth in Decreasing Levels of Heavy Metals in Industrial Wastewater