A model of optimal control of cervical cancer through vaccination and screening Karunia Theda Kristanti1*, Trisilowati2, Agus Widodo2. In this paper, the problem of optimal control of a cervical cancer model with vaccination and screening as controls is discussed.
Different Intestinal Microbiota Composition Inhibits the Lactobacillus Growth in Rheumatoid Arthritis Patients in Malang, Indonesia
The amount of aerobic mesophilic microbiota in faecal samples from control and RA patients. This showed that the types of microbes in the digestive tract in the RA group were more diverse.
Bitter Melon (Momordica charantia L.) and Star fruit (Averrhoa bilimbi L.) on Proinflammatory Cytokines Produced by Hyperglycemic Mice Model
The administration of SBME in P2 with doses of 40 mg.kg-1 BW significantly reduced the production of IL-1b pro-inflammatory cytokine by CD4 T cells. SBME with specific doses can be used to reduce inflammation in patients with hyperglycemia because it can reduce the relative number of macrophage cells that produce IL-1b pro-inflammatory cytokines.
Characterization of Probiotics Isolated from Intestine of Mackerel Fish (Rastrelliger sp.) from Lembata Regency of East Nusa Tenggara
Lactic acid bacteria growing are characterized by the formation of clear zones around the colony. Lactic acid bacteria were grown in MRS broth media, while Aeromonas hydrophila and Streptococcus agalactiae were grown in Trypticase Soy Broth (TSB) media [7,16]. Lactic acid bacteria can be found in some saltwater fish that live in tropical seas.
The population of lactic acid bacteria in the aquatic environment is influenced by the temperature and salinity of the freshwater [42]. Lactic acid bacteria have the ability to tolerate environmental conditions due to their ability to produce lysozyme enzymes. As many as eleven isolates of lactic acid bacteria were successfully isolated from the intestines of mackerel.
Radical scavenging capabilities of saba-narezushi, Japanese fermented chub mackerel and its lactic acid bacteria. Screening of potential probiotic lactic acid bacteria and amylase production and its partial purification. The effect of administration of lactic acid bacteria on growth, digestive enzyme activity and intestinal microbiota in Persian sturgeon (Acipenser persicus) and beluga (Huso huso) fry.
Virtual Prediction of Phenolic and Glucosinolate Compounds with Keap1 Protein as Anti-aging by Stimulating Nrf2
We obtained the structure of the Nrf2-Keap1 protein complex (ID: 2FLU) from rcsb.pdb.org. Evaluation of drug similarity of four bioactive compounds was based on Lipinski's five rules. Based on the tabular physicochemical characterization of the compounds (Table 1), the four bioactive compounds had molecular weights less than 500, indicating that they are easily transported, dispersed, and absorbed compared to heavy molecules [11].
The bioactivity results of the four bioactive compounds are shown in Table 2. Quercetin and kaempferol had high bioactivity results as kinase inhibitors, nuclear receptor ligands, and enzyme inhibitors. The binding energy itself is the sum of the intermolecular forces on the receptor-ligand complex [22]. The binding results between Keap1 and sulforaphane showed that the ligand did not bind to the active site of the Kelch domain.
The docking results between Keap1 and glucoraphanin showed that the ligand did not bind to the active site of the Kelch domain. It shows that quercetin and glucoraphanin can change the stability of Nrf2-Keap1 interaction. In addition, the presence of hydrophobic bonds can increase the biological activity of the drug [22].
Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study
Effect of water clover (Marsilea crenata) ethanol extracts on follicle and oocyte diameter of goat: in vitro study. Therefore, the present study aimed to evaluate the impact of water clover ethanol extract (WCE) at the different concentrations on the growth of follicles and oocytes based on the follicles and oocyte diameters respectively, after the six days of the antral follicle culture. Morphological evaluation of follicular growth Evaluation of the growth of antral follicles morphology was checked by stereo-microscope (SMZ 645 Nikon, Tokyo, Japan) approved with a camera at x20 magnification and observed on days 0, 3 and 6.
One of the aims of this study was to evaluate the effect of different concentrations of WCE on the growth of isolated cultured antral follicles for six days. Isolated antral follicles in vitro culture in different treatments: the control group (first row), this experimental group supplemented with and 86.4 µg.mL-1 (second, third and fourth row respectively) on day 0, day 3 and day 6 In addition, flavonoids also regulate the activity of the antioxidant enzyme to inhibit cell damage and followed by apoptosis [22].
Antral follicle growth also occurred due to the presence of phytoestrogens in WCE that produce estrogenic activity/agonist. According to our results, WCE exerts its effect on antral follicle and oocyte growth based on follicle and oocyte diameter, respectively, in a dose-dependent manner after the sixth day of in vitro antral follicle culture. In vitro exposure of porcine granulosa cells to the phytoestrogens genistein and daidzein: effects on reproductive steroid biosynthesis.
Plant Growth Promoting Endophytic Bacteria of Coffea canephora and Coffea arabica L. in UB Forest
Possible endophytic bacterial isolates of PGP agents were isolated from chromosomal DNA according to ZR Fungal/ modification. Rahnella aquatilis from the soybean rhizosphere had phosphate solubilizing activities with organic acid release that lower the pH of the culture medium [ 36 ]. Growth stimulation of Jatropha curcas by the plant growth-promoting bacterium Enterobacter cancerogenus MSA2.
Identification and characterization of the endophytic plant growth promoter Bacillus cereus strain mq23 isolated from Sophora alopecuroides root nodules. Malondyladehyde (MDA) is the end result of the lipid peroxidation process, which is toxic to cells. Egg yolk contains LDL (Low-Density Lipoprotein), which can keep sperm at a low temperature, increase motility during storage, and bind to the sperm membrane to protect the membrane from protein damage [8].
The level of ROS production in the sperm population has a negative correlation with sperm quality. This indicates that when the MDA level increases, sperm motility would decrease. Additionally, the interaction between motility and viability indicates that when sperm motility is reduced, viability would also be reduced.
Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia
Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia. Isolation and screening of cellulolytic bacteria Twenty-five grams of sago pit waste was suspended in 225 ml of 0.85% NaCl solution, and we made serial dilution to 10-7. The study of cellulolytic bacteria from the sago pit waste was obtained 21 isolates, where 16 isolates from location A and 5 isolates from location B.
Each bacterial isolate with a density of 107 cells.mL-1 in the exponential growth phase (24-hour incubation) had the highest capacity for cellulase enzyme production. A similar study showed that the exponential growth phase of cellulolytic bacteria occurs at 4-24 hours of incubation. [13]. Species of potential cellulolytic bacteria A potential cellulolytic bacteria isolate A1E from sago kernel waste was identified phylogenetically based on 16S rDNA sequence similarity. An indigenous A1E isolate from sago pulp waste from Palopo, South Sulawesi had the highest potency as a cellulolytic bacteria.
Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity. Biodiversity of indigenous amylolytic and cellulolytic bacteria in sago residues in Susupu, North Moluccas. Isolation and identification of cellulolytic bacteria in fibric, hemic and sapric peats in Teluk Bakung Peatland, Kubu Raya District, Indonesia.
The Use of Food Coloring Dyes in Bacterial Staining
1, the microscopic characteristics of the bacterial cells were mostly clearly observable after the application of food coloring. Red food coloring resulted in outlining the cell boundaries with clear and colorless area internal to the bacterial cells on all Gram-positive and Gram-negative bacteria, as with purple food coloring in Bacillus sp. Cell images of different Gram-positive and negative bacteria stained with different water-based food colors without washing.
Other food dyes were retained in most bacterial cells, regardless of their Gram type, with the most clearly retained food dye in pink food dye and purple food dye. In addition, cell morphology and staphylo-type configuration were observed when stained with pink food dye in S. Images of cells of various Gram-positive and negative bacteria stained with various water-based food dyes and washed after staining.
However, the experiments tend to use pure compounds and usually do not use food dyes directly. In this experiment, food dyes used contain a mixture of sorbitol and one or two dyes. Our findings indicate that it was found that pink and purple food coloring is not only taken up by the bacterial cells, thus enabling the cells to be visible, because the stains were retained after washing.
The Effect of Gamma Irradiation on the Growth and Multiplication of the In Vitro Shoot of Patchouli (Pogostemon cablin Benth.)
Effect of Gamma Irradiation on the Growth and Multiplication of Patchouli (Pogostemon cablin Benth.) in Vitro. The effect of gamma radiation on the percentage of staining, the percentage of explants forming shoots, the number and length of in vitro shoots of patchouli after 4-8 weeks of cultivation. The effect of gamma radiation on explant staining has also been observed in shoot explant of Colocasia esculenta (L.) Schott [18], callus of Ferula gummosa [19] and plant of Gerbera jamesonii [20].
A previous study observed the effect of gamma radiation on the in vitro total phenolic content of Ferula gummosa. The effect of gamma radiation on shoot elongation was dependent on gamma dose and genotype, respectively. Effect of gamma radiation on morphological and physiological variation from individual in vitro shoots of banana cv.
Effect of Gamma Irradiation on In-Vitro Callus Induction and Salinity Stress Selection of Rice (Oryza sativa L.). Effect of gamma radiation on in vitro total phenolic content and antioxidant activity of Ferula gummosa Bioss. Effects of gamma radiation on the growth and propagation of chrysanthemum shoot explants (cv. Yellow Puma) in vitro.
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References typed into numbering list (format number 1,2,3,…), arranged sequentially as they appear in the text (Vancouver system or author number style). In the manuscript, only the reference number is typed (not the author and year), for example: Obesity is an accumulation of fat in large quantities that would cause excessive body weight (overweight) [1]. The conclusions of the research findings are short, concise and solidly written, without any additional new interpretations.
This section can also be written about research novelty, advantages and disadvantages of the research, as well as recommendations for future research. (Calibri 10 Justify). This section describes gratitude to those who helped in kind as well as financially. Importance of CD80/CD86-CD28 interaction in the recognition of target cells by CD8+CD122+ regulatory T cells.
