BAB IV
HASIL DAN PEMBAHASAN 4.1 HASIL EKSTRAVASKULAR METILEN BLUE
1. Hasil nilai absorbansi untuk kurva Metilen Blue
420 PPM0 440 PPM 460 PPM 480 PPM 500 PPM
0.1 0.2 0.3 0.4 0.5 0.6
f(x) = 0.09 x + 0.06 R² = 0.99
KURVA LARUTAN BAKU METILEN BLUE
Konsentrasi (PPM)
ABS
PPM ABS
420 ppm 0.145982
440 ppm 0.246205
460 ppm 0.304452
480 ppm 0.40913
500 ppm 0.502857
R = 0,9938 a = 0,0587 b = 0,0877
y = 0,0587 + 0,0877 x
2. Table nilai AUC
20 25 30 35 40 45 50 55 0
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8
f(x) = − 0.03 x + 1.4 R² = 0.99
KURVA KALIBRASI METILEN BLUE
Linear ()
WAKTU
ABS
Ke = 0,0619 ppm/menit t1/2 = 11,19 menit Vd =100,50 L
Cl = 6,22095 L/menit
AUC = 65,99075 mgL/menit
LAMPIRAN 3. TABEL NILAI LARUTAN BAKU
1. Hasil nilai Panjang gelombang larutan baku
Sampel Panjang gelombang
20 ppm 580.5 nm
60 ppm 580.5 nm
4. Hasil nilai absorbansi untuk kurva larutan baku
20 PPM0 30 PPM 40 PPM 50 PPM 60 PPM
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1
f(x) = 0.13 x + 0.14 R² = 0.86
KURVA LARUTAN BAKU
Linear ()
KONSENTRASI (PPM)
ABS
PPM ABS
20 ppm 0.313147
30 ppm 0.435938
40 ppm 0.431153
50 ppm 0.582364
60 ppm 0.881296
= 0,2mg
ml ×100ml=20mg - Pengenceran :
1. 20 ppm dalam 10 ml VINI=V2N2
V1.200 ppm = 10 ml.20ppm V1 = 1 ml
2. 30 ppm dalam 10 ml VINI=V2N2
V1.200 ppm = 10 ml.30ppm V1 = 1,5 ml
3. 40 ppm dalam 10 ml VINI=V2N2
V1.200 ppm = 10 ml.40ppm V1 = 2 ml
4. 50 ppm dalam 10 m VINI=V2N2
V1.200 ppm = 10 ml.50ppm V1 = 2,5 ml
5. 60 ppm dalam 10 ml VINI=V2N2
V1.200 ppm = 10 ml.60ppm V1 = 3 ml
5. TABEL NILAI EKSTRAVASKULAR METILEN BLUE 1. Hasil nilai absorbansi untuk kurva Metilen Blue
420 PPM0 440 PPM 460 PPM 480 PPM 500 PPM 0.1
0.2 0.3 0.4 0.5 0.6
f(x) = 0.09 x + 0.06 R² = 0.99
KURVA LARUTAN BAKU METILEN BLUE
Konsentrasi (PPM)
ABS
PPM ABS
420 ppm 0.145982
440 ppm 0.246205
460 ppm 0.304452
480 ppm 0.40913
500 ppm 0.502857
R = 0,9938 a = 0,0587 b = 0,0877
y = 0,0587 + 0,0877 x
Perhitungan PPM Larutan Baku Metilen Blue:
Metilen blue 1000 ppm dalam 100 ml:
- Larutan induk:
1000 ppm = mg
ml ×1000ppm 1mg×100ml=100mg
- Pengenceran :
1. 420 ppm = 420ppm
500ppm×50ml
= 8,4 ml 2. 440 ppm = 440ppm
500ppm×50ml
= 8,8 ml 3. 460 ppm = 460ppm
500ppm×50ml
= 9,2 ml 4. 480 ppm = 480ppm
500ppm×50ml
= 9,6 ml 5. 500 ppm = 500ppm
500ppm×50ml
= 10 ml
2. Hasil kadar Metilen Blue
20 25 30 35 40 45 50 55
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8
f(x) = − 0.03 x + 1.4 R² = 0.99
KURVA KALIBRASI METILEN BLUE
Linear ()
WAKTU
ABS
t (s) Absorbansi (A)
Kadar (C) Log (C) t vs log c
5 0.145982 0.995 0.0021 a= 1.4016
10 15 20 25 30 35 40 45 50
0.246205 0.304452 0.40913 0.502857 0.42513 0.302869 0.263509 0.195444 0.154174
2.1380 2.8021 3.9957 5.0645 4.1782 2.7841 2.3353 1.5591 1.0886
0.3300 0.4474 0.6015 0.7045 0.6209 0.4446 0.3683 0.1928 0.0368
b= -0.0269 R= 0.9883
Perhitungan AUC,t1/2, K Larutan Baku Metilen Blue:
- Dosis :
1000 ppm = mg
ml ×1000ppm
= 1mg
ml ×100ml=100mg ad 100 ml 500 ppm = 500ppm
1000ppm×50ml
= 25ml ad50ml - K(slope):
Ke = 2,303 x (-b)
= 2,303 x (-(-0.0269)) = 0,0619 ppm/menit - t1/2:
t1/2 = 0,693 k
= 0,693 0,0619
= 11,19 menit
= 100,50 L - Cl:
Cl = Vd x K
= 100,50 x 0,0619/menit
= 6,22095 L/menit
- AUC:
AUC0- ∞ = D
K x Vd
= 100mg
0,0619/menit x100,50L
= 16,0747153
AUC0-t
1. AUC1 = (C1+C0)
2 ×(t1−t0)
= (0,995+0)
2 ×(5−0)
= 2,4875 μg.menit/ml 2. AUC2 = (C2+C1)
2 ×(t2−t1)
= (2,1380+0,995)
2 ×(10−5)
= 7,8325 μg.menit/ml 3. AUC3 = (C3+C2)
2 ×(t3−t2)
= (2,8021+2,1380)
2 ×(15−10)
= 12,35025 μg.menit/ml 4. AUC4 = (C4+C3)
2 ×(t4−t3)
= (3,9957+2,8021)
2 ×(20−15)
= 16,9945 μg.menit/ml 5. AUC5 = (C5+C4)
2 ×(t5−t4)
= (5,0645+3,9957)
2 ×(25−20)
= 22,6505 μg.menit/ml 6. AUC6 = (C6+C5)
2 ×(t6−t5)
= (4,1782+5,0645)
2 ×(30−25)
= 23,10675 μg.menit/ml 7. AUC7 = (C7+C6)
2 ×(t7−t6)
= (2,7841+4,1782)
2 ×(35−30)
= 17,40575 μg.menit/ml 8. AUC8 = (C8+C7)
2 ×(t8−t7)
= (2,3353+2,7841)
2 ×(40−35)
= 12,7985 μg.menit/ml 9. AUC9 = (C9+C8)
2 ×(t9−t8)
= (1,5591+2,3353)
2 ×(45−40)
= 9,736 μg.menit/ml 10.AUC10 = (C10+C9)
2 ×(t10−t9)
= (1,0886+1,5591)
2 ×(50−45)
=2,4875+7,8325+12,35025+16,9945+22,6505+23,10675+17,40575+12,7985+9,7 36+6,61925
=131,9815 μg.menit/ml
AUC0-t:
= AUCtotal/2
= 131,9815/2
= 65,99075 mgL/menit
