Supplemental Digital Content 2. TLR4 and TLR5 expression on CD14+ monocytes from healthy volunteers with and without incubaDon with paDent sera. TLR4 (A) and TLR5 (B) expression on CD14+ monocytes from healthy volunteers with and without incubaDon with paDent sera. TLR expression was evaluated on healthy PBMCs without added serum (No Serum), maintained in media condiDoned with serum from healthy controls (HC), paDents who had an unevenLul recovery (No SIRS) or paDents who developed SIRS (SIRS).
No Serum HC No SIRS SIRS No SIRS SIRS No SIRS SIRS 0
500 1000 1500 2000 2500
MFI
TLR4
No Serum HC No SIRS SIRS No SIRS SIRS No SIRS SIRS 0
500 1000 1500
MFI
TLR5
Day 1
Pre-‐operaDve Day 2
Day 1
Pre-‐operaDve Day 2
A
B
