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SDC 2 (Figure). Systematic Appraisal of Quality for Observational Research (SAQOR) (adapted from existing quality assessment tools (1))

1) The athlete sample: /4

- The sample is representative of the population from which it was drawn.

O Yes O No

- The source of the sample is clearly stated.

O Yes O No

- Entry criteria is clearly stated.

O Yes O No

- The sample size is appropriate to determine statistical significance for FMD (power calculation is reported).

O Yes O No

2) The control group: /4

- The control group is easily identifiable.

O Yes O No

- The control group is age- and sex-matched.

O Yes O No

- The control group is untrained (stated in the manuscript).

O Yes O No

- Statistical differences between athletes and controls in anthropometrical variables (e.g., height, weight) are reported.

O Yes O No

3) Quality of microvascular function assessment: /3 - Vascular conductance is reported.

O Yes O No

- Percentage increase in perfusion over baseline and/or peak/basal ratio is presented.

O Yes O No

- Reproducibility measures are reported (i.e. coefficient of variation).

O Yes O No

4) Confounding variables: /3

- Specific medications were excluded.

O Yes O No

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- Existing CVD was excluded.

O Yes O No

- Smokers were excluded.

O Yes O No 5) Data: /2

-Explanation for missing data is given.

O Yes O No

-Data are clearly and accurately presented (i.e. mean±SD/SE/CI, exact P values cited).

O Yes O No

Study Quality /16

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