Supplementary Information
Tissue Mn-DPDP – 8 days Mn-PyC3A – 7 days
Rat 1 - Female Rat 2 - Male
Adrenal Glands 0.027 0.014 <LOQ
Aorta <LOQ 0.0067 <LOQ
Blood <LOQ <LOQ <LOQ
Bone/Bone Marrow Sternum 2.8 3.1 0.016
Brain 0.051 0.072 0.0009
Cecum 0.11 0.092 0.013
Colon 0.0098 0.011 <LOQ
Duodenum 0.053 0.066 <LOQ
Epididymus N/A 0.027 <LOQ
Esophogus <LOQ <LOQ <LOQ
Eye <LOQ <LOQ <LOQ
Fat <LOQ <LOQ <LOQ
Harderian Glands <LOQ <LOQ <LOQ
Heart 0.034 0.026 <LOQ
Ileum <LOQ 0.0092 0.0003
Jejunum <LOQ <LOQ 0.0001
Kidney 0.24 0.43 0.0042
Liver 1.7 2.0 0.018
Lung w/ bronchi 0.034 0.035 <LOQ
Lymph Node (Aux) <LOQ <LOQ <LOQ
Mammary Gland <LOQ N/A <LOQ
Ovary (Female) <LOQ N/A <LOQ
Pancreas 0.25 0.29 0.0046
Peripheral Nerve (sciatic) <LOQ <LOQ <LOQ
Peyer's Patches/ GALT <LOQ <LOQ <LOQ
Pituitary <LOQ <LOQ <LOQ
Prostate N/A 0.075 <LOQ
Salivary Gland - sublingual and parotid <LOQ <LOQ <LOQ
Salivary Gland - Submandibular 0.064 0.10 0.0013
Seminal vesicle N/A 0.11 0.0028
Skeletal muscle (rectus femoris) <LOQ 1.2 <LOQ
Skin (base of tail) 0.44 1.0 <LOQ
Spinal cord (cervical) <LOQ <LOQ 0.0003
Spinal cord (lumbar) 0.10 0.040 0.0016
Spinal cord (thoracic) <LOQ <LOQ 0.0004
Spleen 0.017 0.024 <LOQ
Stomach 0.10 0.11 0.0015
Testes N/A 0.14 0.0004
Thymus / Parathyroid Glands 0.018 0.024 <LOQ
Trachea <LOQ <LOQ <LOQ
Urinary Bladder <LOQ <LOQ <LOQ
Uterus + Vagina 0.025 N/A <LOQ
Total Tissue recovery 6.2 9.1 0.058
Table S1. Percentage of injected dose (%ID) in various organs recovered 8 days after injection of [
52Mn]Mn-
DPDP, including tissues not shown in Table 2 in the main text. Data shown as mean ± SD. N/A = not applicable,
LOQ = limit of quantification.
2
Tissue or excreta 1 day biodistribution
(%ID)
1 day (N)
7 days biodistribution
(%ID)
7 days (N)
Adrenal Glands 0.0002 ± 0.0004 11 0.0002 ± 0.0004 11
Aorta <LOQ 11 <LOQ 11
Blood 0.0002 ± 0.0007 11 0.0001 ± 0.0002 11
Bone/Bone Marrow
Sternum 0.085 ± 0.11 11 0.035 ± 0.029 11
Brain 0.0040 ± 0.0010 11 0.0020 ± 0.0014 11
Cecum 0.55 ± 0.52 11 0.0035 ± 0.0043 11
Colon 0.83 ± 0.68 11 0.017 ± 0.021 11
Duodenum 0.017 ± 0.0077 11 0.0011 ± 0.0011 11
Epididymus 0.0063± 0.0026 6 0.0023 ± 0.0004 5
Esophogus 0.0001 ± 0.0002 11 <LOQ 11
Eye 0.0000 ± 0.0001 11 <LOQ 11
Fat 0.024 ± 0.079 11 0.014 ± 0.045 11
Harderian Glands 0.0000 ± 0.0001 11 <LOQ 11
Heart 0.0014 ± 0.0012 11 0.0002 ± 0.0002 11
Ileum 0.031 ± 0.022 11 0.0006 ± 0.0009 11
Jejunum 0.040 ± 0.025 11 0.0008 ± 0.0007 11
Kidney 0.051 ± 0.026 11 0.0017 ± 0.0013 11
Liver 0.078 ± 0.022 11 0.043 ± 0.014 11
Lung w/ bronchi 0.0013 ± 0.0011 11 0.0002 ± 0.0002 11
Lymph Node (Aux) <LOQ 11 <LOQ 11
Mammary Gland <LOQ 5 <LOQ 6
Ovary <LOQ 5 0.0001 ± 0.0001 6
Pancreas 0.0081 ± 0.0045 11 0.0018 ± 0.0011 11
Peripheral Nerve
(sciatic) 0.0002 ± 0.0007 11 <LOQ 11
Peyer's Patches/
GALT 0.0000 ± 0.0001 11 <LOQ 11
Pituitary <LOQ 11 <LOQ 11
Prostate 0.0024 ± 0.0016 6 0.0005 ± 0.0006 5
Salivary gland - sublignal and parotid
0.0051 ± 0.0038 11 0.0020 ± 0.0015 11
Salivary gland
-submandibular 0.0062 ± 0.0056 11 0.0018 ± 0.0012 11
Seminal vesicle 0.0043 ± 0.0018 6 0.0019 ± 0.0004 5
Skeletal muscle
(rectus femoris) 0.036 ± 0.098 11 0.0095 ± 0.013 11
Skin (base of tail) 0.089 ± 0.17 11 <LOQ 11
Spinal cord
(cervical) <LOQ 11 0.0001 ± 0.0001 11
Spinal cord
(lumbar) <LOQ 11 <LOQ 11
Spinal cord
(thoracic) <LOQ 11 0.0001 ± 0.0001 11
Spleen 0.0009 ± 0.0015 11 0.0003 ± 0.0003 11
Stomach 0.11 ± 0.29 11 0.0028 ± 0.0015 11
Testes 0.0061 ± 0.0044 6 0.0020 ± 0.0006 5
Thymus /
Parathyroid Glands 0.0003 ± 0.0006 11 0.0001 ± 0.0001 11
Trachea <LOQ 11 0.0001 ± 0.0001 11
Urinary Bladder 0.0002 ± 0.0004 11 0.0001 ± 0.0004 11 Uterus + Vagina
(Female) <LOQ 5 0.0001 ± 0.0002 6
Total recovered
from tissues 2.0 ± 1.1 11 0.14 ± 0.11 11
3
GI contents 0.57 ± 0.51 11 0.0024 ± 0.0031 11
Urine 71 ± 4.8 11
Feces 20 ± 4.4 11
Total Recovery 93 ± 3.2 11
