PhysioEx Lab Report
Exercise 1: Cell Transport Mechanisms and Permeability Activity 3: Simulating Osmotic Pressure
Name: Dinara Jasmine Afra Date: 25 September 2023
Session ID: session-cb927884-e780-8bfd-0219-220a8d39571d
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Experiment Results
Predict Questions
Stop & Think Questions
Which of the following is true of osmosis?
You correctly answered: It is a type of diffusion.
1
Which of the following occurs when a hypertonic solution is added to cells?
You correctly answered: The cells shrink.
2
The variable that affects osmotic pressure is
You correctly answered: the concentration of nondiffusing solutes.
3
The net movement of water would be into the cell in a You correctly answered: hypotonic solution.
4
Predict Question 1: What effect do you think increasing the Na Cl concentration will have?
Your answer: increased osmotic pressure.
1 + -
Predict Question 2: What do you think will be the pressure result of the current experimental conditions?
Your answer: pressure above the right beaker.
2
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Experiment Data
NumberRun Solute MWCO Start Conc. L
(mM)
Pressure L (mm
Hg)
Start Conc. R
(mM)
Pressure R (mm
Hg)
(mM/sec)Rate
1 Na Cl 20 5.00 170 0.00 0 0.0000
1 Albumin 20 0.00 170 0.00 0 0.0000
1 Glucose 20 0.00 170 0.00 0 0.0000
2 Na Cl 20 10.00 340 0.00 0 0.0000
2 Albumin 20 0.00 340 0.00 0 0.0000
2 Glucose 20 0.00 340 0.00 0 0.0000
3 Na Cl 50 10.00 0 0.00 0 0.0167
3 Albumin 50 0.00 0 0.00 0 0.0000
3 Glucose 50 0.00 0 0.00 0 0.0000
4 Na Cl 100 0.00 136 0.00 0 0.0000
4 Albumin 100 0.00 136 0.00 0 0.0000
4 Glucose 100 8.00 136 0.00 0 0.0000
5 Na Cl 100 0.00 0 0.00 0 0.0000
5 Albumin 100 0.00 0 0.00 0 0.0000
5 Glucose 100 8.00 0 8.00 0 0.0000
6 Na Cl 200 0.00 0 0.00 0 0.0000
6 Albumin 200 0.00 0 0.00 0 0.0000
6 Glucose 200 8.00 0 0.00 0 0.0036
7 Na Cl 200 0.00 153 0.00 0 0.0000
7 Albumin 200 9.00 153 0.00 0 0.0000
7 Glucose 200 0.00 153 10.00 0 0.0044
Post-lab Quiz Results
Why do you think there is no pressure change?
You correctly answered: Sodium is able to diffuse through the pores.
1
Why do you think there was no pressure change?
You correctly answered: There is no net movement of water because the solute concentrations are the same.
2
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You scored 100% by answering 4 out of 4 questions correctly.
Review Sheet Results
If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will
You correctly answered: double.
1
Which membrane did not allow Na Cl to pass through?
You correctly answered: 20 MWCO.
2 + -
When a solute is able to diffuse through a membrane You correctly answered: equilibrium is reached.
3
Water diffuses
You correctly answered: toward solutes.
4
Explain the effect that increasing the Na Cl concentration had on osmotic pressure and why it has this effect. How well did the results compare with your prediction?
Your answer:
peningkatan osmotic pressure dikarenakan tingginya konsentrasi molekul.
1 + -
Describe one way in which osmosis is similar to simple diffusion and one way in which it is different.
Your answer:
persamaan : tidak membutuhkan energi
perbedaan : di osmosis hanya air yang dapat lewat melalui permeable membrane, kalau simple diffusion ionnya.
2
Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases milliosmoles.”
Your answer:
air akan mengalir dari miliosmoles yang sedikit ke yang banyak, jadi air mengikuti area yang memiliki milliosmoles tinggi.
3
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The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO membrane in place. Explain the results. How well did the results compare with your prediction?
Your answer:
my answer is wrong.
4
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