PhysioEx Lab Report
Exercise 1: Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Diffusion)
Name: Dinara Jasmine Afra Date: 12 September 2023
Session ID: session-b1bd0637-f8fc-6ee2-7811-507fb43e9bbc
Pre-lab Quiz Results
You scored 100% by answering 3 out of 3 questions correctly.
Experiment Results
Predict Questions
Stop & Think Questions
The driving force for diffusion is
You correctly answered: the kinetic energy of the molecules in motion.
1
In diffusion, molecules move
You correctly answered: from high concentration to low concentration.
2
Which of the following dialysis membranes has the largest pore size?
You correctly answered: 200 MWCO.
3
Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane?
Your answer: No, not at all.
1
Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mol.
Which of the following will be able to diffuse through the 200 MWCO membrane?
Your answer: glucose only.
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Experiment Data
Solute MWCO Left Solute
(mM) Right Solute
(mM) Average
Diffusion (mM/sec)
Na Cl 20 9.00 0.00 0.0000
Urea 20 0.00 0.00 0.0000
Albumin 20 0.00 0.00 0.0000
Glucose 20 0.00 0.00 0.0000
Na Cl 20 0.00 0.00 0.0000
Urea 20 9.00 0.00 0.0000
Albumin 20 0.00 0.00 0.0000
Glucose 20 0.00 0.00 0.0000
Na Cl 50 9.00 0.00 0.0150
Urea 50 0.00 0.00 0.0000
Albumin 50 0.00 0.00 0.0000
Glucose 50 0.00 0.00 0.0000
Na Cl 50 18.00 0.00 0.0273
Urea 50 0.00 0.00 0.0000
Albumin 50 0.00 0.00 0.0000
Glucose 50 0.00 0.00 0.0000
Na Cl 100 9.00 0.00 0.0150
Urea 100 0.00 0.00 0.0000
Albumin 100 0.00 0.00 0.0000
Glucose 100 0.00 0.00 0.0000
The reason sodium chloride didn't diffuse left to right is that You correctly answered: the membrane pore size was too small.
1
Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes at the 20 MWCO because
You correctly answered: glucose and albumin are both too large to pass.
2
The rate of diffusion for urea
You correctly answered: is slower than that for sodium because urea is a larger molecule.
3
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Glucose 100 0.00 0.00 0.0000
Na Cl 100 0.00 0.00 0.0000
Urea 100 9.00 0.00 0.0094
Albumin 100 0.00 0.00 0.0000
Glucose 100 0.00 0.00 0.0000
Na Cl 200 0.00 0.00 0.0000
Urea 200 0.00 0.00 0.0000
Albumin 200 0.00 0.00 0.0000
Glucose 200 9.00 0.00 0.0042
Na Cl 200 0.00 0.00 0.0000
Urea 200 0.00 0.00 0.0000
Albumin 200 9.00 0.00 0.0000
Glucose 200 0.00 0.00 0.0000
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Review Sheet Results
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The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to
You correctly answered: increase the rate of diffusion.
1
Describe the difference between the rate of diffusion seen for sodium and urea.
You correctly answered: Urea diffused more slowly because it is larger than sodium.
2
Which of the following solutes did not pass through any of the membranes?
You correctly answered: albumin.
3
When diffusion stops, we say the solution has reached You correctly answered: equilibrium.
4
Describe two variables that affect the rate of diffusion.
Your answer:
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the size of the molecule and the concentration gradient.
Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction?
Your answer:
because the molecule was too large to pass through the pores.
2
Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction?
Your answer:
after the experiment, it was proven that the glucose is the only one that pass through the 200 MWCO membrane.
3
Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea.
Your answer:
sodium chloride, glucose, urea, albumin.
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