Extended Abstract SEMINAR NASIONAL SAINSTEK 2018 Denpasar, Bali – 18 Oktober 2018
1
POTENSI FRAKSI n-HEKSANA EKSTRAK DAUN CENDANA (SANTALUM album, L) SEBAGAI ANTIINFLAMASI TERHADAP MODEL DERMATITIS KONTAK IRITAN PADA TELINGA TIKUS
YANG DIDINDUKSI MINYAK CROTON
Ni Made Puspawati1§, Wiwik Susanah Rita1
1Program Studi Kimia, FMIPA Universitas Udayana
§Email: nmpuspa_65@yahoo.co.id
ABSTRACT
Santalum album Linn (Cendana) has been traditionally used to treat inflammation. This research aimed to evaluate topical anti-inflammatory activity of n hexane extract of S.album leaves on croton-oil induced rat ear edema. The antiinflammatory activity test was done using 25 Wistar rats which divided randomly into five groups. Group 1 (negative control, croton oil 35%), group 2 (positive control, hydrocortisone drug 2.5%), groups 3 4, and 5 treated with 10, 20, and 40 mg/ear of n-hexane extracts respectively along with the croton oil. The thickness of rat ear-edema was measured manually using micrometre after 6 hours of observation. The topical application of n-hexane extract of S.album leaf at doses 10 and 20 mg/ear, and positive control hydrocortisone significantly reduced croton-oil induced rat ear oedema with percentage inhibition of 36, 52, and 73 % respectively. However, at a higher dose of 40 mg/ear, it did not significantly inhibit inflammation as compare to negative control. Histology analysis result also revealed that n hexane extract at doses 10 and 20 mg/ear, and hydrocortisone decreased significantly infiltration of leukocytes. Based on this result, it can be concluded that n-hexane extract of Santalun album Linn leaf exerted a great potency as topical antiinflammatory agent in a dose dependent manner.
Keywords: antiinflammatory, croton oil, Santalum album Linn, topical.
INTRODUCTION
Iritant contact dermatitis [ICD], is an occupational skin diseases promoted by work materials or workflows such as solvents, detergents,degreasing agents, dust, and other substances [1]. Current antiinflammatory compounds available to treat ICD are both steroid and non streoid antiinflmmatory produced various side effects [2]. Thus, the search for a safer alternatives antiinflammatory drug derived from plants is necessary. n-hexane fraction of Santalum album L (cendana) extract demonstrated potent oral antiinflammatory activity in hind-paw rat oedema induced by carragenan therefore it is worth to evaluate whether it can also be used topically [3].
AIM
This research aimed to evaluate topical antiinflammatory activity of n-hexane fraction of cendana leaves extract on rats ear oedema induced by croron oil.
METHODS
Cendana leaves powder was extracted at room temperature using methanol to yield crude methanol extract. The crude methanol extract was fractionated into n-hexane. The n-hexane fraction was subjected to topical antiinflammatory test [4]. The topical anti-inflammatory activity test was done using 25 Wistar rats which were randomly divided into five groups. Group 1, negative control received only croton oil 35% in acetone, group 2, positive, control treated with standard drug (10 mg/ear
2 hydrocortisone cream 2.5%), groups 3,4, and 5 treated with different doses (10, 20, and 40 mg/ear) of n-hexane extract along with croton oil respectively. The thickness of rat ear-edema was measured manually using micrometre after 6 hours of observation.
RESULTS
The topical application of n-hexane extract of S.album leaf at doses 10 and 20 mg/ear, and positive control hydrocortisone significantly reduced croton-oil induced rat ear oedema with percentage inhibition of 36, 52, and 73 % respectively. The effeect of n-hexane extract at 20 mg/ear was comparable to drug hydrocortisone cream as statistical analysis demostrated insignificanly different.
However, at a higher dose of 40 mg/ear, it did not produced significant effect in inhibiting inflammation as compared to negative control. Histology analysis result also revealed that n hexane extract at doses 10 and 20 mg/ear, and hydrocortisone decreased significantly infiltration of leukocytes
CONCLUSION
It can be concluded that n-hexane extract of Santalun album Linn leaf exerted a great potency as topical antiinflammatory agent in a dose dependent manner.
ACKNOWLEDGEMENT
The author wish to thank The Faculty of Mathematic and Natural Sciences for funding this research
REFERENCES [1]
[2]
[3]
English, J.S.C., 2004. Current Concept of Iritant Contact Dermatitis, Occup.Environ Med 61:722-726.
Satyam, S.M., Bairy, K.L., Musharraf, S., Fernandes, D.L. 2014 Inhibition of Croton Oil- Induced Oedema in Rat Ear. Farmacologyonline 3: 22-25.
Puspawati, NM, Suirta, I.W., Wahyuni. N.L.P. M, Ratnayani, K.,2017 Uji Aktivitas Antiinflamasi Fraksi n-Heksan Daun Cendana (Santalum album, L) Terhadap Oedem Tikus Putih Jantan Galur Wistar yang Diinduksi Karagenan Cakra Kimia 5 (2):112-117 [4] Oliveira, R.B., Chagas-Paula, D.A., Secatto, A., Gasparoto, T.H., Faccioli, L.H.,
Campanelli, A.P., Da Costa, F.B. 2013. Topical anti-inflammatory activity of yacon leaf extracts. Braz.J.Farmacon, 23(3): 497-505
Potensi Fraksi n-Heksana Ekstrak Daun Cendana (Santalum album Linn.) Sebagai Antiinflamasi Terhadap Model Dermatitis Kontak Iritan Pada Telinga Tikus Wistar yang Diinduksi Minyak Croton
Oleh
Ni Made Puspawati
Wiwik Susanah Rita
DERMATITIS KONTAK IRITAN AKUT BAHAN KIMIA (phorbol
ester, alkohol, asam kuat , basa kuat, lauril
sulfat pada obat jerawat, sabun,
sampo)
• Pelepasan sitokin,
kemokin, hasil vasodilatasi dan infiltrasi sel (limfosit, eosinophil, makrofag, neutrophil dan sel T) ke dalam epidermis dan dermis.
• kerusakan epidermis → eritema, menebal, udem, bentuk spongiosis dan bergelembung kecil.
Obat:
antiinflamasi
Apakah fraksi n-heksana ekstrak daun cendana mempunyai
aktivitas antiinflamasi yang diberikan secara topikal pada model dermatitis kontak iritan tikus galur Wistar yang diinduksi minyak croton?
Apakah fraksi n-heksana ekstrak daun cendana mampu
menurunkan infiltrasi leukosit pada model dermatitis kontak iritan tikus galur Wistar yang diinduksi minyak croton?
Rumusan Masalah
TUJUAN
Menentukan aktivitas antiinflamasi fraksi n-heksana yang diberikan secara topikal terhadap model dermatitis kontak iritan pada telinga tikus Wistar yang diinduksi minyak croton
Menganalisis gambaran histopatologi infiltrasi sel radang
pada jaringan udema telinga tikus Wistar yang diinduksi
minyak croton
Metode Penelitian
Ekstraksi • Pelarut Metanol
Partisi • n-heksan
Skrining Fitokimia
Uji Aktivitas Antiinflamasi
Identifikasi
Metode Penelitian
30 ekor tikus jantan
wistar
Kontrol Positif
(Hidrokortison + croton oil 35%)
Kontrol Negatif (Croton oil 35%)
Perlakuan 1
(10 mg/ear + croton oil 35%) Perlakuan 2
(20 mg/ear + croton oil 35%) Perlakuan 3
(40 mg/ear+ croton oil %)
Dibagi menjadi 5 kelompok
Kontrol Normal
Hasil dan Pembahasan
Hasil Ekstraksi dan Partisi
Ekstrak pekat metanol Fraksi n-heksana
Hasil dan Pembahasan
Hasil Skrining Fitokimia
Uji Fitokimia
Pereaksi Perubahan Warna/End
apan
Hasil Kesimpul
an
Alkaloid Meyer Endapan
putih kekuningan
Tidak ada endapan
-
Steroid Libermann Burchad
Biru sampai hijau
Hijau kehitaman menjadi hijau pekat keunguan
+
Terpenoid Libermann Burchad
Hijau sampai
ungu
Hijau kehitaman menjadi hijau pekat keunguan
+
Saponin Pengocoka n + HCl
Busa tetap Tidak ada busa -
Fenolik FeCl3 Biru sampai
hitam
Hitam +
Flavonoid Mg + HCl Merah,
Kuning, Jingga, Biru
Biru -
