LEMBAR
HASIL PENILAIAN SEJAWAT SEBIDANG ATAU PEER REVIEW KARYA ILMIAH : PROSIDING
Judul Jurnal Ilmiah (Artikel) : Growth characteristics of mangrove seedling in silvofishery pond – the allometric relationship of height, diameter and leaf abundance
Nama/ Jumlah Penulis : Endah Dwi Hastuti, Rini Budi Hastuti/ 2 orang Status Pengusul : penulis ke-1
Identitas Jurnal Ilmiah : a. Judul Prosiding : The 3rd International Conference on Biological Sciences and Biotechnology (ICBSB)
b. Nomor ISSN : ISSN:1755-1307; E-ISSN:1755-1315 c. Tahun terbit, tempat
pelaksanaan
: 23–24 August 2017, Medan, Indonesia d.
Penerbit/ organiser
: IOP Conference Series: Earth and Environmental Science/ Departemen Biologi Universitas Udayana Medan
e. DOI artikel (jika ada) : doi :10.1088/1755-1315/130/1/012015 f. Alamat web prosiding : https://iopscience.iop.org/article/10.1088/1755-
1315/130/1/012015/pdf g. Terindeks : SCOPUS, SJR 2018: 0.17 Kategori Publikasi Prosiding : Prosiding Forum Ilmiah Internasional Bereputasi**
(beri pada kategori yang tepat) Prosiding Forum Ilmiah nasional
Hasil Penilaian Peer Review :
Komponen Yang Dinilai Nilai Reviewer
Nilai Rata-rata Reviewer 1 Reviewer 2
a. Kelengkapan unsur isi prosiding (10%) 3,00 3,00 3,00
b. Ruang lingkup dan kedalaman pembahasan (30%)
8,20 9,00 8,60
c. Kecukupan dan kemutakhiran data/informasi dan metodologi (30%)
8,50 9,00 8,75
d. Kelengkapan unsur dan kualitas penerbit (30%)
8,20 8,00 8,10
Total = (100%) 27,90 29,00 28,45
Nilai Pengusul = 60% x 28,45 = 17,07 17,07
Semarang, 20 Januari 2020 Reviewer 1
Prof. Dr. Tri Retnaningsih Soeprobowati, MAppSc NIP. 196404291989032001
Unit Kerja : Departemen Biologi - FSM UNDIP
Reviewer 2
Prof. Dr. Hermin Pancasakti Kusumaningrum, SSi, MSi NIP. 197002081994032001
Unit Kerja : Program Studi Bioteknologi, Departemen Biologi - FSM UNDIP
LEMBAR
HASIL PENILAIAN SEJAWAT SEBIDANG ATAU PEER REVIEW KARYA ILMIAH : PROSIDING
Judul Jurnal Ilmiah (Artikel) : Growth characteristics of mangrove seedling in silvofishery pond – the allometric relationship of height, diameter and leaf abundance
Nama/ Jumlah Penulis : Endah Dwi Hastuti, Rini Budi Hastuti/ 2 orang Status Pengusul : penulis ke-1
Identitas Jurnal Ilmiah : a. Judul Prosiding : The 3rd International Conference on Biological Sciences and Biotechnology (ICBSB)
b. Nomor ISSN : ISSN:1755-1307; E-ISSN:1755-1315 c. Tahun terbit, tempat
pelaksanaan
: 23–24 August 2017, Medan, Indonesia d.
Penerbit/ organiser
: IOP Conference Series: Earth and Environmental Science/ Departemen Biologi Universitas Udayana Medan
e. DOI artikel (jika ada) : doi :10.1088/1755-1315/130/1/012015 f. Alamat web prosiding : https://iopscience.iop.org/article/10.1088/1755-
1315/130/1/012015/pdf g. Terindeks : SCOPUS, SJR 2018: 0.17 Kategori Publikasi Prosiding : Prosiding Forum Ilmiah Internasional Bereputasi**
(beri pada kategori yang tepat) Prosiding Forum Ilmiah nasional
Hasil Penilaian Peer Review :
Komponen Yang Dinilai
Nilai Maksimum Prosiding
Nilai Akhir Yang Diperoleh Internasional
Bereputasi
Nasional
a. Kelengkapan Unsur Isi prosiding (10%) 3.00 3,00
b. Ruang lingkup dan kedalaman pembahasan (30%)
9.00 8,20
c. Kecukupan dan kemutahiran
data/informasi dan metodologi (30%)
9.00 8,50
d. Kelengkapan unsur dan kualitas terbitan/prosiding (30%)
9.00 8,20
Total = (100%) 30.00 27,90
Nilai Pengusul = 60% x 27,90= 16,74 Catatan Penilaian artikel oleh Reviewer :
1. Kesesuaian dan kelengkapan unsur isi prosiding:
Lengkap sesuai guideline, abstrak, keyword, introduction, methods, result discussion, acknowledgment, reference.
2. Ruang lingkup dan kedalaman pembahasan:
Penelitian tentang fisiologi pertumbuhan mangrove. Laju pertumbuhan mangrove berbeda anatar spesies dan perlakukan. Peranan laju pertubmuhan ini untuk tambak silvofishery bagaimana?
3. Kecukupan dan kemutakhiran data/informasi dan metodologi:
Cukup mutakhir, 88% (22/25) referensi berusia kurang atau sama dengan 5 tahun. Metode tidak terlalu rinci, tidak ada analisis data
4. Kelengkapan unsur dan kualitas terbitan:
Paper dipresentasikan The 3rd International Conference on Biological Sciences and Biotechnology 23-24 Agustus 2017, yang diselenggarakan oleh Departemen Biologi Universitas Udayana Medan. Internasional Board Prof. Alison Roberts, Ph.D University of Rhode Island, USA, dengan scientific committee dari Praha, UB, dan lainnya dari USU.
diterbitkan dalam IOP Conference Series: Earth and Environmental Science 130 011003. IOP terindeks SCOPUS, SJR 0,17 (2018), dan H indeks 14
Semarang, 20 Januari 2020 Reviewer 1
Prof. Dr. Tri Retnaningsih Soeprobowati, MAppSc NIP. 196404291989032001
Unit Kerja : Departemen Biologi - FSM UNDIP
LEMBAR
HASIL PENILAIAN SEJAWAT SEBIDANG ATAU PEER REVIEW KARYA ILMIAH : PROSIDING
Judul Jurnal Ilmiah (Artikel) : Growth characteristics of mangrove seedling in silvofishery pond – the allometric relationship of height, diameter and leaf abundance
Nama/ Jumlah Penulis : Endah Dwi Hastuti, Rini Budi Hastuti/ 2 orang Status Pengusul : penulis ke-1
Identitas Jurnal Ilmiah : a. Judul Prosiding : The 3rd International Conference on Biological Sciences and Biotechnology (ICBSB)
b. Nomor ISSN : ISSN:1755-1307; E-ISSN:1755-1315 c. Tahun terbit, tempat
pelaksanaan
: 23–24 August 2017, Medan, Indonesia d.
Penerbit/ organiser
: IOP Conference Series: Earth and Environmental Science/ Departemen Biologi Universitas Udayana Medan
e. DOI artikel (jika ada) : doi :10.1088/1755-1315/130/1/012015 f. Alamat web prosiding : https://iopscience.iop.org/article/10.1088/1755-
1315/130/1/012015/pdf g. Terindeks : SCOPUS, SJR 2018: 0.17 Kategori Publikasi Prosiding : Prosiding Forum Ilmiah Internasional Bereputasi**
(beri pada kategori yang tepat) Prosiding Forum Ilmiah nasional
Hasil Penilaian Peer Review :
Komponen Yang Dinilai
Nilai Maksimum Prosiding
Nilai Akhir Yang Diperoleh Internasional
Bereputasi
Nasional
a. Kelengkapan Unsur Isi prosiding (10%) 3.00 3,00
b. Ruang lingkup dan kedalaman pembahasan (30%)
9.00 9,00
c. Kecukupan dan kemutahiran
data/informasi dan metodologi (30%)
9.00 8,00
d. Kelengkapan unsur dan kualitas terbitan/prosiding (30%)
9.00 9,00
Total = (100%) 30.00 29,00
Nilai Pengusul = 60% x 29,00 = 17,40 Catatan Penilaian artikel oleh Reviewer :
1. Kesesuaian dan kelengkapan unsur isi prosiding: Penulisan sudah sesuai dengan “Guide for Author” (Title, Introduction, Materials and methods, Results and Discussion, Conclution, Acknowledgement, References) dengan sistem Author. Substansi artikel sesuai bidang ilmu pengusul/penulis pertama (Ilmu Tanaman). Ada benang merah dalam struktur penulisannya (skor = 3,00).
2. Ruang lingkup dan kedalaman pembahasan: Substansi artikel sesuai dengan ruang lingkup jurnal Kedalaman pembahasan cukup baik (11 dari 25 bh rujukannya dilibatkan dalam proses membahas hasil) (skor = 9,00).
3. Kecukupan dan kemutakhiran data/informasi dan metodologi: Data-data hasil penelitian belum menunjukkan ada kebaruan informasi. Dari 25 bh rujukannya, terdapat 2 bh sudah kadaluwarsa lebih dari 10 th terakhir, rujukan berupa jurnal ada 5 buah, Proses review cukup baik (skor = 8,00).
4. Kelengkapan unsur dan kualutas terbitan: Jurnal ini tergolong prosiding Internasional Bereputasi (Kontributor lebih dari 4 negara, ISSN: 1742-6588; E-ISSN:1742-6596, terindeks di scopus/SJR= SJR 2018: 0.221 H Indeks :65; coverage 2005-on going ; proses editorial baik (skor = 9,00).
Semarang, 20 Januari 2020 Reviewer 2
Prof. Dr. Hermin Pancasakti Kusumaningrum, SSi, MSi NIP. 197002081994032001
Unit Kerja : Program Studi Bioteknologi, Departemen Biologi - FSM UNDIP
6/14/2020 Effect of incubation time of sago (metroxylon sago) waste by local microorganism ''ginta'' on ph, crude protein, and crude fiber content - I…
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012022 1/4
NOTICE: Ensuring subscriber access to content on IOPscience throughout the coronavirus outbreak - see our remote access guidelines.
PAPER • OPEN ACCESS
Effect of incubation time of sago (metroxylon sago) waste by local microorganism ''ginta'' on ph, crude protein, and crude fiber content
Nurzainah Ginting and E Pase
Published 1 March 2018 • Published under licence by IOP Publishing Ltd
IOP Conference Series: Earth and Environmental Science, Volume 130, The 3rd International Conference on Biological Sciences and Biotechnology 23–24 August 2017, Medan, Indonesia
Abstract
This study aims to examine the effect of incubation times of sago waste by local microorganism (MOL)
"Ginta" to the crude protein and crude fiber content in relation to finding a cheap and good quality ruminants feed alternative. Incubation times were 0 hours to 144 hours. The data obtained were analyzed using Completely Randomize Design consisting of seven treatments and three replications.
The result showed that the duration of incubation of sago waste by local microorganism (MOL) "Ginta"
This site uses cookies. By continuing to use this site you agree to our use of cookies. To find out more,
see our Privacy and Cookies policy.
1 1
Animal Production Program Study, University of Sumatera Utara Jl. Dr. A. Sofian No.3, Padang Bulan, Kampus USU, Medan 20155, Indonesia
Nurzainah Ginting and E Pase 2018 IOP Conf. Ser.: Earth Environ. Sci. 130 012022 https://doi.org/10.1088/1755-1315/130/1/012022
Buy this article in print
1
Help
6/14/2020 Effect of incubation time of sago (metroxylon sago) waste by local microorganism ''ginta'' on ph, crude protein, and crude fiber content - I…
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012022 2/4
[1]
[2]
[3]
[4]
[5]
[6]
caused pH reduction, improved crude protein and crude fiber content. pH reduction was from 7.03 at 0 hour to 4.05 at 144 hours incubation. The highest increased in crude protein was H6U3 (5.58%) : 144 hours incubation and the lowest was H0U2 (3.22%) : 0 hour incubation while the highest crude fiber was H0U1 (19.99%) : 0 hour incubation and the lowest was H6U3 (18.23%) : 144 hours incubation. It can be concluded that incubation of sago waste triggered lower pH, higher crude protein and lower crude fiber than uninoculated. A recommendation could be given on using MOL 'Ginta" in order to produce a cheap and good quality ruminans feed alternative.
Export citation and abstract
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
References
Vikineswary S, Shim JJ. Thambirajah and Blakebrough N 1994 Possible microbial utilization of sago processing waste J. Resources, Conservation and Recycling 11 289-296 30 June 1994 Crossref Google Scholar
Rumalatu 1981 Potensi dan Pemanfaatan Sagu (Yogyakarta: Kanisius) Google Scholar
BPS-Statistic Indonesia 2015 Production Of Sago by Province (ton) 1993-2015 Google Scholar
Flach M 1997 Proc. Sago Conference in Serawak (Malaysia) Yield Potential of The Realisation Google Scholar
abdul Aziz 2002 Sago starch and its utilization Journal of Bioscience and Bioengineering 94 526- 529 http://doi.org/cjwwkx.
Crossref Google Scholar
Trisnowati and Kurniawati 1991 (Bogor Institute of Agriculture) Kecernaan in vitro Ampas Sagu Metroxylon yang Diperlakukan Secara Biologis Undergraduate Thesis
BibTeX RIS
Help
6/14/2020 Effect of incubation time of sago (metroxylon sago) waste by local microorganism ''ginta'' on ph, crude protein, and crude fiber content - I…
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012022 3/4
[7]
[8]
[9]
[10]
[11]
[12]
[13]
[14]
[15]
Google Scholar
Gazpersz V 1991 Metode Rancangan Percobaan (Bandung: CV Armico) Google Scholar
AOAC 2005 Official Methods of Analysis of AOAC International (AOAC International) Google Scholar
Dertli, Enes and Hilmi Ahmet 2017 Microbial diversity of traditional kefir grains and their role on kefir aroma LWT-Food Science and Technology 89 151-157 Part A, November 2017
Crossref Google Scholar
Campbell JR, Kenealy MD and Campbell KL 2006 Animal Science 4 (New York: McGraw-Hill) Google Scholar
Araújo DD, Alessandro B Amorim, Mayra AD Saleh, Felipe C, Pedro L. Perdig_on, Silvio J Bicudo and Dirlei A Berto 2016 J of Animal Nutrition 2 149-153
Crossref Google Scholar
Sunarlim R, Abubakar Triyantini, Poeloengan M and Setiyanto H 1999 Peningkatan Teknologi Pembuatan Inokulum Mikroba Pengolahan Dadih untuk Menunjang Agroindustri Pedesaan (Laporan Penelitian Balai Penelitian Ternak Bogor TA 1998/1999)
Google Scholar
Tampuebolon 2009 Seminar Nasional Kebangkitan Peternakan (Semarang) Google Scholar
Najafpour D Ghasem 2007 CHAPTER 14-Single-Cell Protein J Biochemical Engineering and Biotechnology 332-341
Crossref Google Scholar
Nurhayati 2010 Bungkil Sawit dan Onggok Pakan Ternak Berkualitas http://www.polteklampung.ac.id/home/index.php?
option=com_content&view=aticle&id=88%3Apenelitian&catid=27%3Apenelitian&Itemid=6.
Accessed on [Sunday, 21 March 2010].
Google Scholar
Export references: BibTeX RIS
Help
6/14/2020 Effect of incubation time of sago (metroxylon sago) waste by local microorganism ''ginta'' on ph, crude protein, and crude fiber content - I…
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012022 4/4
Related content
JOURNAL ARTICLES
Somatic embryogenesis of sago palm (Metroxylon sagu Rottb.) through suspension culture technique Somatic embryogenesis of sago palm (Metroxylon sagu Rottb.) from different origins in Indonesia
Nutrition quality test of fermented waste vegetables by bioactivator local microorganisms (MOL) and effective microorganism (EM4)
Half-life measurements of the Pm isotope with γ-spectroscopy
The Optimation of Crude Fiber Content of Diet for Fattening Madura Beef Cattle to Achieve Good A:P Ratio and Low Methane Production
Suppression of Titanium Disilicide Formation on Heavily Arsenic-Doped Silicon Substrate
144
Help
6/14/2020 Influence of exercise on plasma ammonia and urea after ingestion beverages of carbohydrate electrolyte - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012020 1/5
NOTICE: Ensuring subscriber access to content on IOPscience throughout the coronavirus outbreak - see our remote access guidelines.
PAPER • OPEN ACCESS
Influence of exercise on plasma ammonia and urea after ingestion beverages of carbohydrate electrolyte
Gusbakti Rusip , Sri Mukti Suhartini and Ang Boon Suen Published 1 March 2018 • Published under licence by IOP Publishing Ltd
IOP Conference Series: Earth and Environmental Science, Volume 130, The 3rd International Conference on Biological Sciences and Biotechnology 23–24 August 2017, Medan, Indonesia
Abstract
Ingestion of beverages with carbohydrate electrolyte during exercise can delay fatigue. Fatigue caused by the decreasing of glycogen deposit source and indefensible reproduced ATP result in the
improvement of IMP and ammonia during fatigue. The aim of this research was to observe the alteration of plasma ammonia and urea before, during and after exercise, after ingestion beverages of carbohydrate - electrolyte. Ten male subjects (age 18-30 years) were subjected to there cycle ergometer This site uses cookies. By continuing to use this site you agree to our use of cookies. To find out more,
see our Privacy and Cookies policy.
1 2 3
Department of Physiology, Faculty of Medicine, Muhammadiyah University of Sumatera Utara Programme doctor, Faculty of Medicine, University of Indonesia
Departement of Exercise Physiology, Faculty of Medicine, University of Sains Malaysia Gusbakti Rusip et al 2018 IOP Conf. Ser.: Earth Environ. Sci. 130 012020
https://doi.org/10.1088/1755-1315/130/1/012020 Buy this article in print
1 2 3
Help
6/14/2020 Influence of exercise on plasma ammonia and urea after ingestion beverages of carbohydrate electrolyte - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012020 2/5
[1]
[2]
[3]
at 60% of VO with a pedal speed of 60 rpm until there is fatigued. The subject was given a drink of carbohydrate-electrolyte at a concentration of 6%, 12% and a flavored water placebo (P) to consume the volume of 3 ml/kg BW every 20 minutes. Blood samples were taken at rest and every 20 minutes until fatigue for analyzing plasma ammonia and urea. Mean exercise until fatigue show that no difference for three beverages. However, plasma ammonia and urea were significantly increase compared before and after exercise (p<0.001). Results of plasma ammonia before exercise for beverages CHO 12% (HC) (31.86±1.93μml/l vs 86.50±5.13μml/l), for CHO 6% (MC)
(33.08±1.43μml/l vs 90.68±3.41μml/l), for no carbohydrate (P) (33.64±1.93μml/l vs 93.12 ± 2.91μml/l). Whereas plasma urea before exercise for beverages CHO 12% (4.75±0.12mmol/l vs 5.44±0.10mmol/l), for CHO 6% (4.88±0.20mmol/l vs 5.22± 0.10mmol/l), for Placebo
(4.88±0.20mmol/l vs 5.54±0.24mmol/l). Conclusions that increase of plasma ammonia of during fatigue, can become the criteria for determining intensity exercise until fatigue results are better than plasma lactate.
Export citation and abstract
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
References
Coyle EF, Hagberg et al. 1983 Carbohydrate feeding during prolonged strenuous exercise can delay fatigue J. App Physiol 33 230-35
Crossref Google Scholar
Gusbakti R 2006 Ingestion of carbohydrate-electrolyte beverage improves exercise performance Biomedical research 17 183-87
Google Scholar
Bergstrom JL and Hultman E 1967 A Study of the glycogen metabolism during exercise in man Scand J Clin and Lab Invest. 19 218-23
Crossref Google Scholar
2max
BibTeX RIS
Help
6/14/2020 Influence of exercise on plasma ammonia and urea after ingestion beverages of carbohydrate electrolyte - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012020 3/5
[4]
[5]
[6]
[7]
[8]
[9]
[10]
[11]
[12]
[13]
Bromberg S and Sahlin B 1989 Adenine nucleotide degradation in human skeletal muscle during prolonged exercise. J Appl Physio. 67 116-22
Google Scholar
Sahlin K, Bromberg S and Ren JM 1989 Formation of inosine monophosphate (IMP) in human skeletal muscle during incremental dynamic exercise Acta Physiol Scand. 136 193-98
Crossref Google Scholar
Adam Z, Malgorzata C, Adam M, Artur G and Jozef L 2015 Central and peripheral fatigue during resistance exercise J Human Kinetics 49 159-69
Crossref Google Scholar
Gorostiaga EM, Navarro-Amezqueta I, Calbet JAL, Sanchez-Medina I, Cusso M, Guerrero M et al. 2014 Blood ammonia and lactate as a marker of muscle metabolite during leg press exercise J.Strength Cond Res. 28 2775-85
Crossref Google Scholar
Rodney J, Snow MF et al. 2000 Effect of carbohydrate ingestion on ammonia metabolism during exercise in humans J App Physiol. 88 1576-80
Crossref Google Scholar
Wagenmakers AJ, Beckers EJ et al. 1991 Carbohydrate supplementation, glycogen depletion, and amino acid metabolism during exercise Am J Physiol Endocrinol metabolism. 260 E883-890 Crossref Google Scholar
Match BJC and Banister EW 1981 Ammonia metabolism in exercise and fatigue: A review Med Sci Sport and Exerc. 15 41-50
Google Scholar
Dudley GA and Terjungn RL 1985 Influence of aerobic metabolism on IMP accumulation in fast- twitch muscle Am J Pysiol. 248 C37-42
Crossref Google Scholar
Graham TE and MacLean DA 1992 Ammonia and amino acid metabolism in human skeletal muscle during exercise Can J Physiol Pharmacol. 70 132-41
Crossref Google Scholar
Babij P, Matthews SM and Rennie MJ 1983 Changes in blood ammonia, lactate and amino acids about workload during bicycle ergometer exercise in man Eur J Appl Physiol Occup Physiol. 50 405-11
Crossref Google Scholar
Help
6/14/2020 Influence of exercise on plasma ammonia and urea after ingestion beverages of carbohydrate electrolyte - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012020 4/5
[14]
[15]
[16]
[17]
[18]
Nybo L, Dalsgaard MK, Steensberg A and Mielke M 2005 Cerebral ammonia uptake and accumulation during prolonged exercise in a human J.Physiol. 563 285-90
Crossref Google Scholar
Brouns F, Beckers E, Wagenmekers AJM and Saris WHM 1990 Ammonia accumulation during highly intensive long-lasting cycling: individual observations Int J Sports Med. 11 S78-S84 Crossref Google Scholar
Fallowfield JL and William C 1993 Carbohydrate intake, and recovery from prolonged exercise Int J Sport Med. 3 150-64
Google Scholar
Yuan Y and Chan KM 2004 A longitudinal study on the ammonia threshold in junior cyclists Br.J.Sports Med. 38 115-19
Crossref Google Scholar
Demura S, Yamada T, Yamaji S, Komatsu M and Morista KC 2010 The effect of L-ornithine hydrochloride ingestion on performance during incremental exhaustive ergometer bicycle exercise and ammonia metabolism during and after exercise Eur.J.Clinical Nutr. 64 1166-71 Crossref Google Scholar
Export references: BibTeX RIS
Related content
JOURNAL ARTICLES
The effect of manual acupuncture on blood neutrophil counts in moderate intensity exercise Electrolytes and electrolysis
Ammonia in breath and emitted from skin
BOOK CHAPTERS
Python and Matplotlib Essentials for Scientists and Engineers: First steps
Some Critical Questions in Biological Physics: The structure of carbohydrates—the perfect chip
Help
6/14/2020 Influence of exercise on plasma ammonia and urea after ingestion beverages of carbohydrate electrolyte - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012020 5/5
Help
6/14/2020 Characterization of lactic acid bacteria from local cow´s milk kefir - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012019 1/6
NOTICE: Ensuring subscriber access to content on IOPscience throughout the coronavirus outbreak - see our remote access guidelines.
PAPER • OPEN ACCESS
Characterization of lactic acid bacteria from local cow´s milk kefir
YS Ismail , C Yulvizar and B Mazhitov
Published 1 March 2018 • Published under licence by IOP Publishing Ltd
IOP Conference Series: Earth and Environmental Science, Volume 130, The 3rd International Conference on Biological Sciences and Biotechnology 23–24 August 2017, Medan, Indonesia
Abstract
One of products from milk fermentation is kefir. It is made by adding kefir grains which are composed of lactic acid bacteria and yeast into milk. The lactic acid bacteria are a group of bacteria that produce antimicrobial substances and able to inhibit the growth of pathogenic bacteria. In this research, the lactic acid bacteria were isolated from Aceh local cow`s milk kefir to determine the genus of the isolates. The methods used in the characterization of lactic acid bacteria are colony morphology, cell morphology, and biochemical tests which includes a catalase test; 5%, 6.5%, and 10% salt endurance tests; 37°C and 14°C temperature endurance tests, SIM test, TSIA test, MR-VP test, and O/F test. Of the four isolates found from the cow's milk kefir, two isolates were confirmed as lactic acid bacteria
1 1 1
Biology Department, Faculty of Mathematics and Sciences, Syiah Kuala University, Banda Aceh 23111, Indonesia
YS Ismail et al 2018 IOP Conf. Ser.: Earth Environ. Sci. 130 012019 https://doi.org/10.1088/1755-1315/130/1/012019
Buy this article in print
1
Help
6/14/2020 Characterization of lactic acid bacteria from local cow´s milk kefir - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012019 2/6
[1]
[2]
[3]
[4]
[5]
[6]
(isolates SK-1 and SK-4). Both isolates are Gram positive bacteria, and have negative catalase activity.
From the observations of colony morphology, cell morphology, and biochemical tests, it was found that the genus of SK-1 is Lactobacillus and the genus of SK-4 is Enterococcus.
Export citation and abstract
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
References
Zakaria Y 2009 Pengaruh Jenis Susu dan Persentase Starter yang Berbeda terhadap Kualitas Kefir Agripet 9 26-30
Crossref Google Scholar
Kosikowski 2011 Kajian Penggunaan Ekstrak Susu Kedelai Terhadap Kualitas Kefir Susu Kambing Jurnal Ternak Tropika 12 15-21
Google Scholar
Usmiati S 2007 Kefir, Susu Fermentasi dengan Rasa Menyegarkan Jurnal Warta Penelitiandan Pengembangan Pascapanen Pertanian 29 12-15 Bogor.
Google Scholar
Albaarri AN and Murti TW 2003 Analisa pH, Keasaman dan Kadar Laktosa pada Yakult, Yoghurt, Kefir Proceeding Simposium Nasional Hasil-hasil Penelitian di Unika Soegijapranata (Semarang, 22 Maret 2003)
Google Scholar
Suryadjaja A 2005 (Bogor: Fakultas Teknologi Pertanian, Institut Pertanian Bogor.) Potensi Ubi Jalar Putih dan Merah (Ipomoea batatas L.) untuk Pertumbuhan Bakteri Asam Laktat dan Menekan Pertumbuhan Patogen Skripsi
Google Scholar
Ulusoy BS, Colak H, Hampikyan H and Erkan ME 2007 An In Vitro Study on the Antibacterial Effect of Kefir Against some Food-borne Pathogens Turk Mikrobioloji Cemiyeti Dergisi 37 103-
BibTeX RIS
Help
6/14/2020 Characterization of lactic acid bacteria from local cow´s milk kefir - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012019 3/6
[7]
[8]
[9]
[10]
[11]
[12]
[13]
[14]
[15]
[16]
107
Google Scholar
Raja A, Gajalakshmi MMM, Raja and Imran MM 2009 Effect of Lactobacillus lactis cremonis Isolated from Kefir against Food Spoilage Bacteria American Food Technology 4 201-209 Crossref Google Scholar
Dorland 2011 Dorland's Illustrated Medical Dictionary Deluxe (London UK) Google Scholar
Morgan GE, Mikhail MS and Murray MJ 2006 Clinical Anesthesiology 4 (New York, USA:
Lange Medical Books/McGraw-Hill) Google Scholar
Suhartanti D and Septian R 2014 Comparison of the Antibacterial Activity of Cow Kefir and Goat Kefir Against Bacteria Bacillus cereus. KESMAS 8 71-76
Google Scholar
Yesillik S, Yildirim N, Dikici A, Yildiz A and Yesillik S 2011 Antibacterial Effects of Some Fermented Commercial and Homemade Dairy Products and 0,9% Lactic Acid against Selected Foodborne Pathogens Asian Journal of Animal and Veterinary Advances 6 189-195
Crossref Google Scholar
Hadioetomo RS 1993 Mikrobiologi Dasar dalam Praktek (Jakarta: Gramedia) Google Scholar
Lay BW 1994 Analisis Mikroba di Laboratorium (Jakarta: Raja Grafindo Persada) Google Scholar
Volk WA and Wheeler MF 1993 Mikrobiologi Dasar Jilid I. Diteijemahkan oleh Markham (Jakarta: PT. Gelora Aksara Pratama)
Google Scholar
Davis WW and Stout TR 1971 Disc Plate Methods of Microbiological Antibiotic Assay Microbiology 22 659-665
Google Scholar
Sudarsono A 2008 (Bogor: Institut Pertanian Bogor) Lepidocibium flavobronneum Isolasi dan Karakterisasi Bakteri pada Ikan Laut dalam Spesies Ikan Gindara Skripsi
Google Scholar
Help
6/14/2020 Characterization of lactic acid bacteria from local cow´s milk kefir - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012019 4/6
[17]
[18]
[19]
[20]
[21]
[22]
[23]
[24]
[25]
[26]
Ernawati 2010 (Malang: Universitas Islam Negeri Maulana Malik Ibrahim) Isolasi dan Identifikasi Bakteri Asam Laktat pada Susu Kambing Segar Skripsi
Google Scholar
Pelczar MJ and Chan ECS 1986 Dasar-dasar Mikrobiologi ed R.S. Diterjemahkan oleh Hadioetomo, T. Imas, S.S. Tjitrosomo and S.I. dan Angka (Jakarta: UI Press.)
Google Scholar
Syahrurachman 1994 Buku Mikrobiologi Kedokteran (Jakarta: Universitas Indonesia Press) Google Scholar
Wibowo and Ristanto D 1988 Petunjuk Khusus Deteksi Mikroba Pangan.Pusat (Yogyakarta:
Antar Universitas Pangan dan Gizi Universitas Gadjah Mada) Google Scholar
Velmurugan K, Sekar A and Palingamoorthy G 2013 Antagonistic Studies of Kefir Bacteria Against Food Pathogens (Germany: LAMBERT Academic Publishing)
Google Scholar
Tadasse G, Ephraim E and Ashenafi M 2005 Assessment of the Antimicrobial Activity of Lactic Acid Bacteria Isolated from Borde and Shamita, Traditional Ethiopian Fermented Beverages, on some Food-borne Pathogens and Effect of Growth Medium on the Inhibitory Activity Journal of Food Safety 8 14-18
Google Scholar
Battcoock M and Ali SA 1998 Fermented Fruits and Vegetables, A Global Perspective (Rome, Italy: FAO Agricultural Services Bulletin No 134.)
Google Scholar
Djide MN and Wahyuddin E 2008 Isolasi Bakteri Asam Laktat dan Air Susu Ibu dan Potensinya dalam Penurunan Kadar Kolestrol Secara In Vitro. Majalah Farmasi dan Farmakologi 12 12-15 Google Scholar
Fugelsang KC and Edwards CG 2007 Wine Microbiology, Practical Applications and Procedures (New York, USA: Springer Science Bussiness Media LLC)
Google Scholar
Guessas B and Kihal M 2004 Characterization of Lactic Acid Bacteria Isolated from Algerian and Zone Raw Goat's Milk African Journal of Biotechnology 3 339-342
Crossref Google Scholar
Help
6/14/2020 Characterization of lactic acid bacteria from local cow´s milk kefir - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012019 5/6
[27]
[28]
[29]
[30]
[31]
[32]
[33]
[34]
[35]
[36]
Kuswanto KR and Sudarmadji S 1988 Proses-proses Mikrobiologi Pangan (Yogyakarta: Pusat Antar Universitas Pangan dan Gizi)
Google Scholar
Vos DP, Garrity GM, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH and Whitman WB 2009 Bergey`s Manual of Systematic Bacteriology (New York, USA: Springer Dordrecht
Heidelberg) Google Scholar
Buckle KA, Edwards RA, Fleet GH and Wootton M 1987 Ilmu Pangan (Jakarta: Diterjemahkan oleh Purnomo dan Adiyono. UI Press.)
Google Scholar
Yoshikazu I, Ikue S and Seiichi S 1994 Identification and Characteristics of Lactic Acid Bacteria Isolated from Masai Fermented Milk in Northern Tanzania Journal Bioscience, Biotechnology, and Biochemistry 58 660-664
Crossref Google Scholar
Fardiaz S 1988 Fisiologi Fermentasi (Bogor: Pusat Antar Universitas Lembaga Sumberdaya Informasi, Instititut Pertanian Bogor)
Google Scholar
Stamer JR 1979 The Lactic Acid Bacteria. Microbes of Diversity J. Food Technology 1 60-65 Google Scholar
Ardhana MM and Graham HF 2003 The Microbial Ecology of Cocoa Bean Fermentation in Indonesia International Journal of Food Microbiology 86 87-99
Crossref Google Scholar
Safriani SR 2015 (Banda Aceh: Universitas Syiah Kuala.) Isolasi dan Karakterisasi Serta Uji Antimikroba Bakteri Asam Laktat dari Fermentasi Susu Kambing Peranakan Etawa Skripsi Google Scholar
Hasan ZH 2006 Isolasi Lactobacillus, Bakteri Asam Laktat dari Feses dan Organ Saluran Pencernaan Ayam Seminar Nasional Teknologi Peternakan dan Veteriner 2006
Google Scholar
Sunaryanto R and Bambang M 2012 Isolasi, Identifikasi dan Karakterisasi Bakteri Asam Laktat dari Dadih Susu Kerbau Jurnal Sains dan Teknologi Indonesia 14 288-233
Google Scholar
Help
6/14/2020 Thermus Thermophilus as a Model System for the Study of Ribosomal Antibiotic Resistance - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012002 1/6
NOTICE: Ensuring subscriber access to content on IOPscience throughout the coronavirus outbreak - see our remote access guidelines.
PAPER • OPEN ACCESS
Thermus Thermophilus as a Model System for the Study of Ribosomal Antibiotic Resistance
Steven T. Gregory
Published 1 March 2018 • Published under licence by IOP Publishing Ltd
IOP Conference Series: Earth and Environmental Science, Volume 130, The 3rd International Conference on Biological Sciences and Biotechnology 23–24 August 2017, Medan, Indonesia
Abstract
Ribosomes are the intracellular ribonucleoprotein machines responsible for the translation of mRNA sequence into protein sequence. As an essential cell component, the ribosome is the target of numerous antibiotics that bind to critical functional sites to impair protein synthesis. Mutations causing resistance to antibiotics arise in antibiotic binding sites, and an understanding of the basis of resistance will be an essential component of efforts to develop new antibiotics by rational drug design. We have identified a This site uses cookies. By continuing to use this site you agree to our use of cookies. To find out more,
see our Privacy and Cookies policy.
1,2
Department of Cell and Molecular Biology, College of the Environment and Life Science The University of Rhode Island Kingston, RI 02881 USA
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University Providence, RI 02912 USA
Steven T. Gregory 2018 IOP Conf. Ser.: Earth Environ. Sci. 130 012002 https://doi.org/10.1088/1755-1315/130/1/012002
Buy this article in print
1
2
Help
6/14/2020 Thermus Thermophilus as a Model System for the Study of Ribosomal Antibiotic Resistance - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012002 2/6
[1]
[2]
[3]
[4]
number of antibiotic-resistance mutations in ribosomal genes of the thermophilic bacterium Thermus thermophilus. This species offers two primary advantages for examining the structural basis of antibiotic-resistance, in particular, its potential for genetic manipulation and the suitability of its ribosomes for analysis by X-ray crystallography. Mutations we have identified in this organism are in many instances identical to those found in other bacterial species, including important pathogens, a result of the extreme conservation of ribosome functional sites. Here I summarize the advantages of this organism as a model system to study antibiotic-resistance mechanisms at the molecular level.
Export citation and abstract
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
References
Williams RA, Smith KE, Welch SG, Micallef J and Sharp RJ 1995 DNA relatedness of Thermus strains, description of Thermus brockianus sp. nov., and proposal to reestablish Thermus
thermophilus (Oshima and Imahori) Int J Syst Bacteriol 45 495-499 Crossref Google Scholar
Brock TD and Freeze H 1969 Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile J Bacteriol 98 289-297
Google Scholar
Oshima T and Imahori K 1974 Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from a Japanese thermal spa Int J Syst Bacteriol 24 102-112
Crossref Google Scholar
Cava F, Hidalgo A and Berenguer J 2009 Thermus thermophilus as biological model Extremophiles 13 213-231
Crossref Google Scholar
BibTeX RIS
Help
6/14/2020 Thermus Thermophilus as a Model System for the Study of Ribosomal Antibiotic Resistance - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012002 3/6
[5]
[6]
[7]
[8]
[9]
[10]
[11]
[12]
[13]
[14]
Koyama Y, Hoshino T, Tomizuka N and Furukawa K 1986 Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp. J Bacteriol 166 338-340
Google Scholar
Henne A, Brüggemann H, Raasch C, Wiezer A, Hartsch T et al. 2004 The genome sequence of the extreme thermophile Thermus thermophilus Nat Biotechnol 22 547-553
Crossref Google Scholar
Kristjansson JK, Hreggvidsson GO and Alfredsson GA 1986 Isolation of halotolerant Thermus spp. from submarine hot springs in Iceland Appl Environ Microbiol 52 1313-1316
Google Scholar
Schmeing TM and Ramakrishnan V 2009 What recent ribosome structures have revealed about the mechanism of translation Nature 461 1234-1242
Crossref Google Scholar
Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT and Ramakrishnan V 2000 Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics Nature 407 340-348
Crossref Google Scholar
Bulkley D, Innis CA, Blaha G and Steitz TA 2010 Revisiting the structures of several antibiotics bound to the bacterial ribosome Proc Natl Acad Sci USA 107 17158-17163
Crossref Google Scholar
Schlünzen F, Zarivach R, Harms J, Bashan A, Tocilj A, Albrecht R, Yonath A and Franceschi F 2001 Structural basis for the interaction of antibiotics with the peptidyl transferase centre in eubacteria Nature 413 814-821
Crossref Google Scholar
Dunkle JA, Xiong L, Mankin AS and Cate JHD 2010 Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action Proc Natl Acad Sci USA 107 17152-17157
Crossref Google Scholar
Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D and Ban N 2017 The complete structure of the Mycobacterium smegmatis 70S ribosome Cell Rep 20 149-160
Crossref Google Scholar
Gale EF, Cundliffe E, Reynolds PE, Richmond MH and Waring MJ 1981 The molecular basis of antibiotic action (London, United Kingdom: John Wiley & Sons) 402-547
Google Scholar
Help
6/14/2020 Thermus Thermophilus as a Model System for the Study of Ribosomal Antibiotic Resistance - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012002 4/6
[15]
[16]
[17]
[18]
[19]
[20]
[21]
[22]
[23]
Gregory ST, Cate JH and Dahlberg AE 2001 Streptomycin-resistant and streptomycin-dependent mutants of the extreme thermophile Thermus thermophilus J Mol Biol 309 333-338
Crossref Google Scholar
Gregory ST and Dahlberg AE 2009 Genetic and structural analysis of base substitutions in the central pseudoknot of Thermus thermophilus 16S ribosomal RNA RNA 15 215-223
Crossref Google Scholar
Gregory ST, Carr JF, Rodriguez-Correa D and Dahlberg AE 2005 Mutational analysis of 16S and 23S rRNA genes of Thermus thermophilus J Bacteriol 187 4804-4812
Crossref Google Scholar
Demirci H, Murphy FV, Murphy EL, Connetti JL, Dahlberg AE, Jogl G and Gregory ST 2014 Structural analysis of base substitutions in Thermus thermophilus 16S rRNA conferring streptomycin resistance Antimicrob Agents Chemother 58 4308-4317
Crossref Google Scholar
Demirci H, Wang L, Murphy FV, Murphy EL, Carr JF, Blanchard SC, Jogl G, Dahlberg AE and Gregory ST 2013 The central role of protein S12 in organizing the structure of the decoding site of the ribosome RNA 19 1791-1801
Crossref Google Scholar
Cameron DM, Thompson J, Gregory ST, March PE and Dahlberg AE 2004 Thiostrepton-resistant mutants of Thermus thermophilus Nucleic Acids Res 32 3220-3227
Crossref Google Scholar
Stanley RE, Blaha G, Grodzicki RL, Strickler MD and Steitz TA 2010 The structures of the antituberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome Nat Struct Mol Biol 17 289-293
Crossref Google Scholar
Monshupanee T, Gregory ST, Douthwaite S, Chungjatupornchai W and Dahlberg AE 2008 Mutations in conserved helix 69 of 23S rRNA of Thermus thermophilus that affect capreomycin resistance but not posttranscriptional modifications J Bacteriol 190 7754-7761
Crossref Google Scholar
Gregory ST, Cate JH and Dahlberg AE 2001 Spontaneous erythromycin resistance mutation in a 23S rRNA gene, rrlA, of the extreme thermophile Thermus thermophilus IB-21 J Bacteriol 183 4382-4385
Crossref Google Scholar
Help
6/14/2020 Thermus Thermophilus as a Model System for the Study of Ribosomal Antibiotic Resistance - IOPscience
https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012002 5/6
[24]
[25]
[26]
Rodriguez-Correa D and Dahlberg AE 2008 Kinetic and thermodynamic studies of
peptidyltransferase in ribosomes from the extreme thermophile Thermus thermophilus RNA 14 2314-8
Crossref Google Scholar
Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC and Ramakrishnan V 2009 Insights into substrate stabilization from snapshots of the peptidyltransferase center of the intact 70S ribosome Nat Struct Mol Biol 16 528
Crossref Google Scholar
Demirci H, Murphy F, Murphy E, Gregory ST, Dahlberg AE and Jogl G 2013 A structural basis for streptomycin-induced misreading of the genetic code Nat Commun 4 1355-1358
Crossref Google Scholar
Export references: BibTeX RIS
Related content
JOURNAL ARTICLES
Prevalence of Antibiotic-resistance Enterobacteriaceae strains Isolated from Chicken Meat at Traditional Markets in Surabaya, Indonesia
Antibiotic resistance: a physicist’s view
Design of dual action antibiotics as an approach to search for new promising drugs Control of ribosome traffic by position-dependent choice of synonymous codons
Prevalence of antibiotic resistance in adult septic patients of H. Adam Malik central general hospital, Medan under Indonesia’s mandatory health scheme
Intra-molecular chaperone: the role of the N-terminal in conformational selection and kinetic control
Help