Supplemental table 1
Gene Forward (5’-3’) Reverse (5’-3’) Product length (bp)
CB1R TCTGCTTGCGATCATGGTGT GCATGTCTCAGGTCCTTGCT 147
CB2R GTCATGTGGGTCCTCTCAGC AGAAGCCAGCCCAGTAGGTA 122
GPR55 AGAGCGTGACAACTGCTCAT AGGTTGAGAACCAGGCCAAG 102
TRPV1 GCATCATCAACGAGGACCCA GGGAACCAGGGCAAAGTTCT 113
PPAR-α ACCTGTGAACACGACCTGAAA CATGTATGACAAAAGGCGGGTT 160
PPAR-γ GATGTCTCACAATGCCATCAGG TTCCTGTCAAGATCGCCCTC 197
DAGL-α GACCTTGTCCCCAGGATTGG TTGGGGATACACTTGGTGGC 125
DAGL-β AGGACTTTGTCGTGTCGCTT AACAGTCCGTACCAACAGCC 157
NAPE-PLD ATCTCCATCCCGAATGTGCT GCTCTTTGTCAAGTTCCTCTTTG 88
MAGL GGAACAAGTCGGAGGTTGAC CATTGCTCGCTCCACTCTTG 116
FAAH CATGGCACGGGATGTGGATA GTATCCCACACGAAGGGGTC 142
CNRIP1 CCAGAACCGCACAATCAAGC GGGACAAGGACACCACCAAT 111
GAPDH TGTGTCCGTCGTGGATCTGA GCCTGCTTCACCACCTTCTTGA 78
Supplemental table 2
gene Mean±SEM (control, LH , LH-R)
Sample size (control, LH , LH-R)
Treatment effect P value Significance
Figure 2a CB1 1.02 ± 0.07, 1.14 ± 0.08, 1.25 ± 0.08 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 2.079 P=0.1402 P > 0.05 CB2 1.07 ± 0.12, 1.10 ± 0.10, 0.85 ± 0.08 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 2.082 P=0.1399 P > 0.05 GPR55 1.04 ± 0.10, 1.10 ± 0.06, 1.10 ± 0.09 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.1532 P=0.8586 P > 0.05 PPAR-α 1.01 ± 0.04, 0.96 ± 0.06, 1.01 ± 0.05 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.3016 P=0.7415 P > 0.05 PPAR-γ 1.03 ± 0.09, 1.15 ± 0.07, 1.27 ± 0.12 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 1.296 P=0.2865 P > 0.05 TRPV1 0.95 ± 0.10, 0.74 ± 0.10, 0.48 ± 0.05 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 9.389 P=0.0005 P < 0.001
Figure 2b CB1 1.10 ± 0.17, 0.94 ± 0.17, 1.05 ± 0.22 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.1514 P=0.8601 P > 0.05 CB2 1.15 ± 0.18, 1.15 ± 0.15, 1.39 ± 0.15 10, 12, 15 One way ANOVA with LSD post hoc test F (2, 34) = 0.8171 P=0.4502 P > 0.05 GPR55 1.08 ± 0.13, 1.21 ± 0.15, 1.10 ± 0.14 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.2222 P=0.8019 P > 0.05 PPAR-α 1.01 ± 0.04, 1.26 ± 0.27, 1.21 ± 0.12 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.5070 P=0.6066 P > 0.05 PPAR-γ 1.07 ± 0.13, 1.15 ± 0.12, 1.10 ± 0.22 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.03411 P=0.9665 P > 0.05 TRPV1 1.26 ± 0.30, 1.03 ± 0.19, 1.41 ± 0.24 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.6478 P=0.5294 P > 0.05
Figure 2c CB1 1.04 ± 0.10, 1.02 ± 0.07, 0.97 ± 0.08 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.1688 P=0.8454 P > 0.05 CB2 1.11 ± 0.11, 1.29 ± 0.10, 1.04 ± 0.11 10, 10, 15 One way ANOVA with LSD post hoc test F (2, 32) = 1.258 P=0.2979 P > 0.05 GPR55 1.03 ± 0.09, 0.88 ± 0.06, 0.71 ± 0.04 10, 11, 15 One way ANOVA with LSD post hoc test F (2, 33) = 8.030 P=0.0014 P < 0.01 PPAR-α 1.04 ± 0.10, 0.96 ± 0.10, 0.93 ± 0.07 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.4365 P=0.6498 P > 0.05 PPAR-γ 1.02 ± 0.07, 0.91 ± 0.06, 0.85 ± 0.10 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.9428 P=0.3992 P > 0.05 TRPV1 1.03 ± 0.08, 1.21 ± 0.12, 1.00 ± 0.10 10, 12, 15 One way ANOVA with LSD post hoc test F (2, 34) = 1.260 P=0.2964 P > 0.05
Figure 2d CB1 1.01 ± 0.06, 0.76 ± 0.05, 0.93 ± 0.08 10, 12, 15 One way ANOVA with LSD post hoc test F (2, 34) = 3.237 P=0.0517 P > 0.05 CB2 1.02 ± 0.04, 1.06 ± 0.09, 1.34 ± 0.17 10, 12, 12 One way ANOVA with LSD post hoc test F (2, 30) = 1.994 P=0.1538 P > 0.05 GPR55 1.02 ± 0.07, 0.67 ± 0.08, 0.74 ± 0.10 10, 12, 15 One way ANOVA with LSD post hoc test F (2, 34) = 4.097 P=0.0255 P < 0.05 PPAR-α 1.04 ± 0.10, 1.18 ± 0.06, 1.25 ± 0.10 10, 12, 13 One way ANOVA with LSD post hoc test F (2, 32) = 1.449 P=0.2498 P > 0.05 PPAR-γ 1.32 ± 0.36, 1.43 ± 0.25, 0.80 ± 0.19 10, 12, 13 One way ANOVA with LSD post hoc test F (2, 32) = 1.756 P=0.1889 P > 0.05 TRPV1 1.11 ± 0.17, 1.05 ± 0.21, 0.72 ± 0.09 10, 12, 11 One way ANOVA with LSD post hoc test F (2, 30) = 1.528 P=0.2334 P > 0.05
Figure 3a DAGL-α 1.01 ± 0.05, 0.79 ± 0.02, 0.88 ± 0.03 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 8.970 P=0.0007 P < 0.001 DAGL-β 1.00 ± 0.04, 0.98 ± 0.04, 1.00 ± 0.04 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.06931 P=0.9332 P > 0.05 NAPE-PLD 1.01 ± 0.04, 0.98 ± 0.03, 0.94 ± 0.04 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.7039 P=0.5015 P > 0.05 MAGL 1.01 ± 0.04, 1.12 ± 0.02, 1.12 ± 0.04 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 3.031 P=0.0611 P > 0.05 FAAH 1.01 ± 0.03, 0.91 ± 0.02, 1.01 ± 0.03 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 3.102 P=0.0575 P > 0.05 CNRIP 1 1.00 ± 0.02, 1.05 ± 0.02, 1.10 ± 0.04 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 2.232 P=0.1224 P > 0.05
Figure 3b DAGL-α 1.02 ± 0.07, 1.97 ± 0.41, 2.58 ± 0.43 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 3.921 P=0.0291 P < 0.05 DAGL-β 1.02 ± 0.07, 1.15 ± 0.15, 1.34 ± 0.23 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.6876 P=0.5095 P > 0.05 NAPE-PLD 1.04 ± 0.09, 1.01 ± 0.13, 1.29 ± 0.17 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 36) = 1.187 P=0.3169 P > 0.05 MAGL 1.01 ± 0.06, 1.18 ± 0.04, 1.36 ± 0.15 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 2.396 P=0.1059 P > 0.05 FAAH 1.02 ± 0.06, 1.13 ± 0.10, 1.47 ± 0.18 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 2.693 P=0.0817 P > 0.05 CNRIP 1 1.01 ± 0.05, 1.17 ± 0.07, 1.30 ± 0.14 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 1.674 P=0.2022 P > 0.05
Figure 3c DAGL-α 1.00 ± 0.03, 0.86 ± 0.04, 0.77 ± 0.03 10, 11, 16 One way ANOVA with LSD post hoc test F (2, 34) = 11.42 P=0.0002 P < 0.001 DAGL-β 1.01 ± 0.05, 0.97 ± 0.04, 0.87 ± 0.04 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 2.757 P=0.0773 P > 0.05
NAPE-PLD 1.04 ± 0.10, 1.03 ± 0.05, 1.09 ± 0.06 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.2759 P=0.7605 P > 0.05 MAGL 1.02 ± 0.07, 0.98 ± 0.03, 0.94 ± 0.03 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.9789 P=0.3858 P > 0.05 FAAH 1.03 ± 0.09, 0.94 ± 0.06, 0.75 ± 0.04 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 5.337 P=0.0095 P < 0.01 CNRIP 1 1.02 ± 0.08, 1.10 ± 0.04, 1.09 ± 0.04 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 0.5723 P=0.5694 P > 0.05
Figure 3d DAGL-α 1.01 ± 0.04, 1.00 ± 0.21, 0.72 ± 0.10 10, 12, 15 One way ANOVA with LSD post hoc test F (2, 34) = 0.2804 P=0.7572 P > 0.05 DAGL-β 1.01 ± 0.05, 0.97 ± 0.04, 0.82 ± 0.04 10, 12, 16 One way ANOVA with LSD post hoc test F (2, 35) = 5.419 P=0.0089 P < 0.01 NAPE-PLD 1.01 ± 0.06, 0.90 ± 0.04, 0.96 ± 0.06 10, 12, 15 One way ANOVA with LSD post hoc test F (2, 34) = 0.9614 P=0.3925 P > 0.05 MAGL 1.01 ± 0.04, 1.12 ± 0.07, 0.98 ± 0.05 10, 12, 14 One way ANOVA with LSD post hoc test F (2, 33) = 2.109 P=0.1374 P > 0.05 FAAH 1.01 ± 0.05, 0.92 ± 0.05, 0.82 ± 0.04 10, 12, 14 One way ANOVA with LSD post hoc test F (2, 33) = 4.369 P=0.0207 P < 0.05 CNRIP 1 1.01 ± 0.04, 1.06 ± 0.03, 1.02 ± 0.02 10, 12, 14 One way ANOVA with LSD post hoc test F (2, 33) = 0.9260 P=0.4062 P > 0.05
