Supplementary Table 2.

Mitochondrial disruption is involved in the effect of NFATc4 on aggravating cardiomyocyte hypertrophy

Running title: NFATc4 compromised mitochondria in hypertrophy

Xueping Liu, PhD ^a,*^,1, Si Gao, PhD ^a,1, Hui Gao, PhD ^b, Xudong Jiang, PhD ^a, Qiqiu Wei, MS^a

a. Department of Pharmacy, School of Medicine, Guangxi University of Science and Technology, Liuzhou, Guangxi, People’s Republic of China; b. Department of pharmacology, School of Medicine, Shaoxing University, Shaoxing, People’s Republic of China.

* Corresponding author, Department of Pharmacy, School of Medicine, Guangxi University of Science and Technology, Liuzhou, Guangxi, People’s Republic of China. Fax number: +86 772 2056019

Email addresses: 100000774@gxust.edu.cn.

1 Xueping Liu and Si Gao contributed equally to this work.

Conflicts of interest

The authors declare no conflicts of interest for this work.

Financial support

This work is supported by the National Natural Science Foundation of China (Nos.

81660042,81500214,81560059,81760058), Natural Science foundation of Guangxi Province (No. 2016GXNSFBA380208), Science Research Project of High education of Guangxi Province (No.KY2016YB243), and excellent teachers abroad study program of High education of Guangxi Province.

Supplementary Table 1. Primers for qRT-PCR analysis

Gene Primer sequences

ANF Forward:5’-CCGTATACAGTGCGGTGTCC-3' Reverse: 5’-CAGAGAGGGAGCTAAGTGCC-3' BNP Forward: 5’-AGCTGCTTTGGGCAGAAGAT-3' Reverse: 5’-AAAACAACCTCAGCCCGTCA-3' UCP2 Forward: 5’-ACCATTGCACGAGAGGAAGG-3'

Reverse: 5’-TCTTGACCACATCAACGGGG-3' NRF-1 Forward: 5’-TGTCACCATGGCCCTTAACAGTGA-3'

Reverse:5’-TGAACTCCATCTGGGCCATTAGCA-3' TFAM Forward:5’-AAATGGCTGAAGTTGGGCGAAGTG-

3'

Reverse: 5’-AGCTTCTTGTGCCCAATCCCAATG-3' EER-α Forward:5’GTGCACATTGAAGATGCTGAGGCT-3' Reverse: 5’TAGAAATGGGCCAGGACTTTGCCT-3' MACD Forward:5’CTGCTCGCAGAAATGGCGATGAAA-3'

Reverse: 5’CAAAGGCCTTCGCAATAGAGGCAA-3' LCAD Forward:5’AATGGGAGAAAGCCGGAGAAGTGA-

3'

Reverse: 5’GATGCCGCCATGTTTCTCTGCAAT-3' ATP-5β Forward:5’TTGCTGAGGTCTTCACAGGTCACA-3' Reverse: 5’CAGCCTTTGCCACAGCTTCTTCAA-3' GLUT1 Forward:5’TATCGTCAACACGGCCTTCACTGT-3' Reverse: 5’CACAAAGCCAAAGATGGCCACGAT-3' PPAR-α Forward:5’AGCTCAGGACACAAGACGTTGTCA-3'

Reverse: 5’AGGGACTTTCCAGGTCATCTGCTT-3' β-actin Forward: 5’-TCGTGCGTGACATTAAAGAG-3'

Reverse: 5’-ATTGCCGATAGTGATGACCT-3'

Supplementary Table 2. Sequence of small RNA for silencing of

NFATc4

Gene sequences

NFATc4

Forward:5'-GGAGUCUGAACUUAAUGAATT-3' Reverse:5'-UUCAUUAAGUUCAGACUCCTT-3'

Supplementary Fig s1

PPAR-α

EER-α

MACD

LCAD

GLUT1  ATP-5 0.0

0.5 1.0 1.5

Vetor

Vector+PE NFATc4 NFATc4+PE

* * *

# # #

* *

Relative mRNA expression ( fold of control)

Fig s1 Effects of NFATc4 overexpression on PGC-1α target genes (The results

were collected from five independent experiments, n=5) Supplementary Fig s2

PPAR-α

EER-α

MACD

LCAD

GLUT1  ATP-5 0.0

0.5 1.0 1.5

NCNC+PE siNFATc4 siNFATc4+PE

* ^# * *

# #

* *

Relative mRNA expression ( fold of control)

Fig s2 Effects of NFATc4 knockdown on PGC-1α target genes (The results were collected from five independent experiments, n=5)