Supplementary figures

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Supplementary figures

Supplementary Figure 1. The partial DNA sequencing results for the SNP in LASS2 gene 3  -UTR.

Red represents the same nucleotide acids sequences as the reference sequence of

LASS2 gene 3  -UTR in gene bank, and green represents the SNP locus. LASS2 gene

rs8444 C>T polymorphism site is located in 136bp of the gene segment in the LASS2

3  -UTR.

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Supplementary Figure 2. Genotypes of the SNP rs8444 locus in LASS2 gene 3  -UTR.

(A)rs8444 CC genotype; (B) rs8444 TC genotype; (C) rs8444 TT genotype.

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Supplementary Tables

Supplementary Table 1. Adjusted odds ratios for relationship between polymorphisms of rs8444 in the 3  -UTR of LASS2 and Clinical-pathological characters of bladder cancer.

rs8444

Clinical stages Histological grade Metastasis

Superficial

（T0-T1） [n, (%)]

Invasive

（T2-T4） [n, (%)]

aOR(95%CI) ^aP

Low grade

（G1） [n, (%)]

High grade

（G2-G3） [n, (%)]

aOR(95%CI) ^aP

Negative [n, (%)]

Positive [n, (%)]

aOR(95%CI) ^aP

Allele

C 37(61.70) 88(58.70) 1.0(Ref) 26(52.00) 99(61.90) 1.0(Ref) 53(58.90) 72(60.00) 1.0(Ref)

T 23(38.30) 62(41.30) 1.201(0.648-2.229) 0.561 24(48.00) 61(38.10) 0.661(0.348-1.255) 0.206 37(41.10) 48(40.00) 0.941(0.538-1.644) 0.830

total 60(100.00) 150(100.00) 50(100.00) 160(100.00) 90(100.00) 120(100.00)

Genotype

CC 9(30.00) 28(37.30) 1.0(Ref) 6(24.00) 31(38.80) 1.0(Ref) 14(31.10) 23(38.33) 1.0(Ref)

TC 19(63.30) 32(42.70) 0.540(0.206-1.416) 0.210 14(56.00) 37(46.20) 0.515(0.176-1.509) 0.226 25(55.60) 26(43.33) 0.637(0.269-1.512) 0.306 TT 2(6.70) 15(20.00) 2.183(0.407-11.712) 0.362 5(20.00) 12(15.00) 0.429(0.108-1.703) 0.229 6(13.30) 11(18.33) 1.078(0.324-3.589) 0.902

total 30(100.00) 75(100.00) 25(100.00) 80(100.00) 45(100.00) 60(100.00)

CI: confidence interval;OR: odds ratio.^aOR and^aPwere adjusted for age, gender and smoking.

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Supplementary Table 2. Clinical pathological parameters of 58 follow-up patients with bladder cancer carrying different rs8444 genotypes.

rs8444 genotype [n, (%)]

Total [n, (%)]

TT (n=10)

TC (n=26)

CC (n=22) Gender

Male 10(21.28) 22(46.81) 15(31.91) 47(100.00)

Female 0(0.00) 4(36.36) 7(63.64) 11(100.00)

Ages (years)

≤50 2(16.67) 5(41.67) 5(41.67) 12(100.00)

＞50 8(17.39) 21(45.65) 17(36.96) 46(100.00)

Tumor stage

T0+T1 2(9.52) 10(47.62) 9(42.86) 21(100.00)

T2+T4 8(21.62) 16(43.24) 13(35.14) 37(100.00)

Tumor grade

G1 3(20.00) 7(46.67) 5(33.33) 15(100.00)

G2+G3 7(16.28) 19(44.19) 17(39.53) 43(100.00)

Tumor metastasis

Negative 5(18.52) 11(40.74) 11(40.74) 27(100.00)

Positive 5(16.13) 15(48.39) 11(35.48) 31(100.00)

Survival

Alive 3(9.38) 12(37.50) 17(53.12) 32(100.00)

Death 7(26.92) 14(53.85) 5(19.23) 26(100.00)