Table S1. Univariate associations with Serum Hepcidin Level

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Table S1. Univariate associations with Serum Hepcidin Level

(*) Ratio reported for a 10-unit increase in explanatory variable (**) Ratio reported for a 5-unit increase in explanatory variable (†) Variable analysed on log scale

eGFR=estimated Glomerular Filtration rate; hsCRP=high sensitivity C-Reactive Protein;

TSAT=Transferrin saturation; ACEI=Angiotensin Converting Enzyme Inhibitor; ARB=Angiotensin Receptor Blocker; MMF=Mycophenolate Mofetil; AZA=Azathioprine; FTI=Fat Tissue Index Variable Category Univariate

Coefficient (95% CI)

Univariate P-value

eGFR (ml/min) * Linear term 0.69 (0.57, 0.84) 0.001 Squared term 1.03 (1.01, 1.05)

hsCRP (mg/l)^† - 1.15 (1.07, 1.24) 0.001

TSAT (%) * 1.25 (1.11, 1.40) 0.001

ACEI/ARB Yes 1.22 (1.03, 1.44) 0.02

MMF/AZA Yes 1.31 (0.55, 3.12) 0.04

FTI (kg/m²) ** - 1.07 (1.00, 1.14) 0.05 Gender Male 0.96 (0.81, 1.14) 0.63 Age (years) * - 1.00 (0.94, 1.07) 0.93 Time post

transplant (yr) **

- 1.05 (0.98, 1.11) 0.14

Rejection Yes 1.05 (0.85, 1.30) 0.62

Diabetes Yes 1.11 (0.89, 1.38) 0.40

Albumin (g/dl) 0.99 (0.96, 1.02) 0.47 Statin therapy Yes 0.96 (0.68, 1.35) 0.33

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Table S2. Multivariate associations with Serum Hepcidin Level

(*) Ratio reported for a 10-unit increase in explanatory variable (†) Variable analysed on log scale

TSAT=Transferrin saturation; ACEI=Angiotensin Converting Enzyme Inhibitor; ARB=Angiotensin Receptor Blocker; MMF=Mycophenolate Mofetil; AZA=Azathioprine

Variable Category Multivariate Coefficient (95% CI)

Multivariate P-value

eGFR (ml/min) * Linear term 0.73 (0.61, 0.87) 0.001 Squared term 1.02 (1.01, 1.03)

hsCRP (mg/l)^† - 1.13 (1.06, 1.21) 0.001

TSAT (%) * 1.27 (1.15, 1.41) 0.002

ACEI/ARB Yes 1.18 (1.02, 1.37) 0.03

MMF/AZA Yes 1.16 (1.03, 1.31) 0.03

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Table S3. Univariate associations with hsCRP

(*) Ratio reported for a 5-unit increase in explanatory variable (**) Ratio reported for a 10-unit increase in explanatory variable

TSAT=Transferrin saturation; ACEI=Angiotensin Converting Enzyme Inhibitor; ARB=Angiotensin Receptor Blocker; MMF=Mycophenolate Mofetil; AZA=Azathioprine; FTI=Fat Tissue Index Variable Category Univariate

Univariate P-value

FTI (kg/m²) * Linear term 0.77 (0.38, 1.56) 0.009 Squared term 1.11 (1.06, 1.17)

Gender Male 0.96 (0.81, 1.14) 0.15 Diabetes Yes 1.35 (1.03, 1,77) 0.03 ACEI/ARB Yes 1.26 (0.89, 1.78) 0.21 MMF/AZA Yes 0.95 (0.58, 1.56) 0.83 eGFR (ml/min) ** - 0.98 (0.90, 1.07) 0.68 Age (yr) ** - 0.94 (0.84, 1.06) 0.30 Time post

transplant (yr) *

- 1.05 (0.93, 1.17) 0.45

Rejection Yes 0.74 (0.50, 1.10) 0.13 Statin therapy Yes 0.90 (0.62, 1.31) 0.27

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Table S4. Multivariate associations with hsCRP

(*) Ratio reported for a 5-unit increase in explanatory variable; FTI=Fat Tissue Index Variable Category Multivariate

Multivariate P-value

0.01

0.04 FTI (kg/m²) * Linear term 0.80 (0.48, 1.33)

Squared term 1.10 (1.03, 1.17)

Gender Male 1.44 (1.09, 1.91)

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Table S5. Univariate associations with Haemoglobin

(*) Coefficients reported for a 20-unit increase in explanatory variable (**) Coefficients reported for a 10-unit increase in explanatory variable (***) Coefficients reported for a 5-unit increase in explanatory variable (†) Variable analysed on log scale

TSAT=Transferrin saturation; ACEI=Angiotensin Converting Enzyme Inhibitor; ARB=Angiotensin Receptor Blocker; MMF=Mycophenolate Mofetil; AZA=Azathioprine; FTI=Fat Tissue Index

Variable Category Univariate beta

Univariate P-value Hepcidin (ng/ml)* Linear term -1.07 (-1.42, -0.73) 0.009

Squared term 0.08 (0.04, 0.12)

eGFR (ml/min) ** - 0.31 (0.20, 0.42) 0.002 Gender Male 0.57 (0.16, 0.98) 0.01 hsCRP (mg/l)^† - -0.21 (-0.38, -0.04) 0.03

ACEI/ARB Yes -0.48 (-0.89, -0.07) 0.03

MMF/AZA Yes -0.42 (-0.70, -0.14) 0.04

FTI (kg/m²) *** - -0.04 (-0.21, 0.13) 0.62 Age (yr) ** - -0.02 (-0.17, 0.13) 0.79 Time post

transplant (yr) ***

- -0.11 (-0.27, 0.04) 0.15

Rejection Yes -0.07 (-0.60, 0.46) 0.79 TSAT (%) *** -0.13 (-0.42, 0.16) 0.39 Diabetes Yes -0.33 (-0.88, 0.12) 0.23 Albumin (g/dl) -0.02 (-0.09, 0.05) 0.58 Statin therapy Yes 0.06 (-0.56, 0.68) 0.44

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Table S6. Multivariate associations with Haemoglobin

(*) Coefficients reported for a 20-unit increase in explanatory variable (**) Coefficients reported for a 10-unit increase in explanatory variable eGFR=estimated Glomerular Filtration rate

Variable Category Multivariate beta

Multivariate P-value Hepcidin (ng/ml)* Linear term -1.12 (-1.44, -0.79) 0.009

Squared term 0.09 (0.06, 0.13)

eGFR (ml/min) ** - 0.22 (0.12, 0.31) 0.002 Gender Male 0.57 (0.24, 0.90) 0.008