ALP (U/L)]
3.7. Storage conditions, stability, and reproducibility assessment
The fabricated SPCE/AuNPs/Au-nano-dendroid/GO/anti-ALP biosensor probe was then stored at 4o C after its fabrication and the sensors were used for the determination of ALP after every week and the Rct values were recorded. The Rct responses obtained by the sensor probe shows that it retained the sensitivity of 98.03% until 8th week, while the significant decrement of the signal was observed afterwards, which is most likely due to
the loss of biological activity of anti-ALP and/or the degradation of the chemical modification steps. The considerably good shelf life up to eight weeks was most likely due to the stable immobilization of anti-ALP and retention of its biological activity on SPCE sensor chip (figure 3.13).
Figure 3.13: Time dependent response of the fabricated SPCE/AuNPs/ Au-nano- dendroid /GO/Anti-ALP sensor probe.
The reproducibility of the fabricated SPCE/AuNPs/Au-nano-dendroid/GO/anti-ALP biosensor was evaluated, which showed the RSD below than the < 2.3% even when the same fabrication process was followed, indicating the sensor fabrication is highly reproducible, and this variation is most likely due to the negligible deviation in the sensor fabrication steps and handling errors.
4. Conclusions:
We have developed a screen-printed based biosensor for the determination of ALP in clinical serum using the Au-nano-dendroid - GO based nanocomposites. The developed nanocomposite was characterized using various techniques including TEM, SAED, EDX, and XRD. Thereafter, SPCE/AuNPs/ Au-nano-dendroid/GO/Anti-ALP sensor was
fabricated by following sequential deposition of the nanocomposite onto the SPCE chip.
The fabricated SPCE/AuNPs/ Au-nano-dendroid /GO/Anti-ALP sensor probe is capable of delivering the ALP detection with wide range of concentration (100 - 1000 U/L) covering the ALP levels in various pathophysiological conditions (viz. bone disease, liver disease, carcinoid syndrome, etc.). The limit of detection was obtained to be 9.10 (±0.12) U/L. The biosensor showed high selectivity towards ALP even in presence of various coexisting molecules including, albumin, globulin, alanine, glucose, citric acid, uric acid and glutamic acid (ksel<< 0.08). The shelf life of the biosensor has also been evaluated, where the sensor probe was found to be stable up to 8 weeks. Apart from these, the fabricated biosensor has many attractive features such as its robustness, easy fabrication, and deliverable at the point of care use.
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