TO STUDY THE ADSORPTION AND DESORPTION CHARACTERISTICS OF ANTHOCYANIN EXTRACTED FROM
4.5. Summary
The present study has provided insights of adsorption, desorption and diffusion characteristics of anthocyanin from purple rice bran extract using XAD2, XAD4, XAD7, activated charcoal and bentonite as adsorbents. The conclusions are summarized as follows:
The amberlite XAD7 resin showed higher adsorption (1.9 mg/g) and desorption (1.8 mg/g) capacities than the other adsorbents. The amberlite XAD7 also showed the highest recovery (41.5 %) of anthocyanin from purple rice bran extract than other adsorbents.
The adsorption of anthocyanin was homogeneous in nature. The two parameter Langmuir isotherm and three-parameter Redlich–Peterson isotherm model can efficiently describe the adsorption isotherm behavior of anthocyanin on various adsorbents.
The adsorption kinetics of anthocyanin was best fitted with pseudo-first order kinetic model than pseudo-second order kinetic model. The XAD7 showed the highest reaction rate constant (0.02 /min) than other adsorbents.
The diffusion study revealed that intra-particle diffusion is not the sole mechanisms of anthocyanin adsorption process onto adsorbents as shown by the Weber and Morris intra-particle diffusion model.
The thermodynamic properties of anthocyanin adsorption process onto XAD7 was successfully investigated. From the ΔH values (-40.3 kJ/mol), it was implied that the adsorption of anthocyanin onto the XAD7 resin was exothermic in nature.
The concentrated sample showed a higher amount of cyanidin-3-glucoside (1279.9 µg/L), peonidin-3-glucoside (68.9 µg/L) and phenolic acids than crude extract.
The degradation behavior of anthocyanins was followed the first-order kinetics model.
The highest rate of degradation for the monomeric anthocyanin, C3G and P3G were observed at 6 pH (11×10-4 /min, 4.9×10-4 /min, and 3.8×10-4 /min) and 90°C (12.8×10-
4 /min, 5.8×10-4 /min, and 6×10-4 /min).
The hydrocolloids showed a significant effect of the stability on monomeric anthocyanin, C3G, and P3G in various pH and temperature condition.
Modified rice starch showed a promising effect on the stability of anthocyanins and antioxidant activity than the other hydrocolloids. Moreover, in modified starch medium total anthocyanin, C3G, and P3G showed the lower degradation rate constant and higher half-life as compared to without hydrocolloid sample.
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