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BT-07

June - Examination 2019 BSC - Pt. II Examination Microbial Biotechnology

Paper - BT-07

Time : 3 Hours ] [ Max. Marks :- 35

Note: The question paper is divided into three sections A, B and C.

Write answers as per the given instructions.

{ZX}e : ¶h àíZ nÌ "A', "~' Am¡a "g' VrZ IÊS>m| ‘| {d^m{OV h¡& à˶oH$

IÊS> Ho$ {ZX}emZwgma àíZm| Ho$ CÎma Xr{OE&

Section - A 7 × 1 = 7

(Very Short Answer Questions)

Note: Answer all questions. As per the nature of the question delimit your answer in one word, one sentence or maximum up to 30 words. Each question carries 1 mark.

IÊS> - "A' (A{V bKw CÎmar¶ àíZ)

{ZX}e : g^r àíZm| Ho$ CÎma Xr{OE& Amn AnZo CÎma H$mo àíZmZwgma EH$ eãX, EH$ dm³¶ ¶m A{YH$V‘ 30 eãXm| ‘| n[agr{‘V H$s{OE& à˶oH$ àíZ 1 A§H$ H$m h¡&

642

BT-07 / 200 / 4 (1) (P.T.O.)

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642

BT-07 / 200 / 4 (2) (Contd.)

1) (i) What is “Frankia”

’«o$pÝH$Am ³¶m h¢?

(ii) Define “Plasmid”

""ßbmpÁ‘S>'' H$mo n[a^m{fV H$s{OE&

(iii) Write two application of recombinant DNA technology.

nwZ©¶moOr DNA VH$ZrH$s Ho$ Xmo AZwà¶moJ {b{IE&

(iv) Who discovered “Polymerase Chain Reaction” (PCR)?

~hþbH$ l¥§Ibm A{^{H«$¶m H$mo gd©àW‘ {H$gZo ImoOm?

(v) Write a note on “Penicillin”

""no{Z{gbrZ'' na {Q>ßnUr {b{IE&

(vi) Define “Fermentation”

""{H$ÊdZ'' n[a^m{fV H$s{OE&

(vii) What is single cell protein?

""EH$b H$mo{eH$m àmoQ>rZ'' ³¶m h¡?

Section - B 4 × 3.5 = 14 (Short Answer Questions)

Note: Answer any four questions. Each answer should not exceed 200 words. Each question carries 3.5 marks.

IÊS> - ~ (bKw CÎmar¶ àíZ)

{ZX}e : {H$Ýht Mma àíZm| Ho$ CÎma Xr{OE& Amn AnZo CÎma H$mo A{YH$V‘

200 eãXm| ‘| n[agr{‘V H$s{OE& à˶oH$ àíZ 3.5 A§H$m| H$m h¡&

(3)

BT-07 / 200 / 4 (3) (P.T.O.) 642

2) Describe Fed batch culture.

""’¡$S> ~¡M g§dY©Z'' H$mo g‘PmBE&

3) Discuss advantage and disadvantage of producing and using microbial biomass.

gyú‘OrdmUw O¡d g§h{V Ho$ CËnmXZ Ed‘² Cn¶moJ Ho$ bm^ d hm{Z g‘PmBE&

4) Explain “Recombinant DNA Technology”

nwZ©¶moOr VH$ZrH$s H$mo g‘PmBE&

5) Explain about DNA finger printing.

S>rEZE q’$Ja qà{Q>J H$mo g‘PmBE&

6) Write note on (i) Clicitors

(ii) Secondary metabolites

{ZåZ na {Q>ßnUr {b{IE&

(i) BbrgrQ>g©

(ii) {ÛVr¶H$ CnM¶mnM¶H$

7) Discuss tools and techniques of Genetic engineering in brief.

AmZwd§{eH$ A{^¶mpÝÌH$s Ho$ gmYZ d VH$ZrH$s H$s {ddoMZm H$s{OE&

8) “Transposons” Explain it.

""Q´>mÝgnmogmoZ'' ñnîQ> H$s{OE&

9) Write note on

(i) Genomic Library (ii) c DNA Library

{Q>ßnUr H$[aE&

(i) OrZmo{‘H$ bmB~«oar

(ii) c DNA bmB~«oar

(4)

642

BT-07 / 200 / 4 (4)

Section - C 2 × 7 = 14 (Long Answer Questions)

Note: Answer any two questions. You have to delimit your each answer maximum up to 500 words. Each question carries 7 marks.

IÊS> - g (XrK© CÎmar¶ àíZ)

{ZX}e : {H$Ýht Xmo àíZm| Ho$ CÎma Xr{OE& Amn AnZo CÎma H$mo A{YH$V‘ 500 eãXm| ‘| n[agr{‘V H$s{OE& à˶oH$ àíZ 7 A§H$m| H$m h¡&

10) What is hairy root culture? How can these be developed for producing secondary metabolites on a commercial scale.

amo{‘b ‘yb g§dY©Z ³¶m h¡? ì¶mdgm{¶H$ ñVa na {ÛVr¶H$ CnM¶mnM¶ËnmX àmßV H$aZo Ho$ {bE BÝh| H¡$go ~Zm¶m OmVm h¡?

11) Write note on / {Q>ßnUr H$[aE&

(i) Antibiotics / à{VO¡{dH$

(ii) Vaccines / Q>rH$m

(iii) Immobilized enzyme in commercial processes

{ZpíM{bV EÝOmB‘ ì¶mdgm{¶H$ CÔoí¶ Ho$ {bE

12) Write an account of industrial manufacture of Alcoholic Beverages

EëH$moh{bH$ no¶ Ho$ Am¡Úmo{JH$ CËnmXH$ na boI {b{IE&

13) Write notes on.

(i) Important fermented food (ii) Biological nitrogen fixation.

{Q>ßnUr H$[aE&

(i) ‘hËdnyU© {H${UdV ImÚnXmW©

(ii) O¡{dH$ ZmBQ´>moOZ pñWarH$aU

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