Centre for Research in Nanoscience and Nanotechnology
University of Calcutta
Technology Campus, Sector-III, Block-JD2, Salt Lake Kolkata 700 098, India
Tender Number: Dir/008/NIT/UGC/instruments/CRNN (2015) dated February 25, 2015
Sub: Tender for Major Instruments at CRNN
Sealed tenders in two bids system (Technical Bid & Financial bid) are invited from bonafide, resourceful and eligible manufacturers/exclusive distributors for the following instruments listed below.
1. Inverted Fluorescent Microscope
2. Microplate based Spectrophotometer & Microdrop plate
3. Mini Vertical gel apparatus ,Western Blotting Module , Fast Blotting System and Power Supply apparatus
4. Gel Documentation system
5. Specimen Freezing Microtome / Cryostat
6. Double-barrier Langmuir-Blodgett Normal Trough 7. Chiller for High Pressure Equipment
8. Lyophilyser (Freeze Dryer) 9. Vacuum Pumping System 10. Electrochemical Workstation
11. Minus 20°C DEEP FREEZER (400 L)
Part – I (Technical Bid) of the tender should contain technical details and commercial terms and conditions and Part – II (Price Bid) should indicate group-wise price as mentioned in the Technical Bid where applicable. The Technical Bid and Price bid are to be submitted in two separately sealed envelopes distinctly marked accordingly and both to be put inside another envelope, which should be sealed and super scribed with tender notice no. and due date. The bidders may submit bids duly signed in their own letterheads.
All tenders should reach to the office of The Director, Centre for Research in Nanoscience and Nanotechnology (CRNN), University of Calcutta, JD-2, Sector-III, Salt Lake City, Kolkata-700098 before March 16, 2015 in one sealed envelope.
The tender notice number along with the serial number and name of the instrument for which the tender is being submitted should clearly be written on the envelope.
The technical bids will be opened first to evaluate the technical specifications of the equipment thereafter, the Price bids of only technically qualified bidders will be opened.
The opening date of technical as well as price bid is March 18, 2015.
Required Specifications for the Instruments
1. Required Specifications for ‘Inverted Fluorescent Microscope’
Single compact integrated unit including: cell imaging system, digital camera, computer, high power fluorescence lighting system and LCD or LED display.
Should have 3 independent high output LED illuminators with integrated hard coated fluorescence bandpass excitation and emission filters for each cube or better.
Fluorescence LED light cube must be a single, interchangeable cube can be easily removed and installed
Fluorescence LED illuminators must have a lifetime of 50,000 hours at 100% power or better
System must automatically recognize which cube is installed and adjust the software configuration accordingly.
LED illuminators must have independent intensity control.
System must be able to simultaneously accommodate 4 fluorescent LED light cubes
Should be integrated high-sensitivity monochrome interline CCD camera with 1.4 million 6.45um pixels or better
System must be able to accommodate a minimum of 5 objectives at once which can be user defined OR must include 20x, 40x, 60x and 100x phase contrast fluorite objectives
System should have rack and pinion “glide” stage with SBS microplate opening and Accessory vessel holders
System must be able to sequentially acquire a phase contrast image and a fluorescence image with mouse click, then overlay them automatically.
System must include time lapse imaging capability with automatic movie creation.
System must include manual Cell counting capability.
System must provide the following output file formats: jpg, bmp, tiff, png or others
System should have onstage CO2 incubator to handle various samples for cell culture.
System should have Co2 controller unit as well as software to control Co2 percentage within incubator.
Co2 Incubator should allow precise specimen temperature adjustment.
It should allow stable and smooth ambient temperature control.
2. Required Specifications for ‘Microplate based Spectrophotometer & Microdrop plate’
A spectral scanning unit for UV-visible-Near IR wavelength range, should be able to measure 96 & 384 microwell plate format and for low volume sample plate.
A monochromator based wavelength selection using Xenon Flash lamp as light source for better performance.
Instrument should able to provide the wavelength range from 200nm to 1000nm with 1 nm steps.
Spectral scanning speed should be: maximum 10 sec from 200 to 1000 nm with 1 nm steps per sample.
Performance Specifications :
Wavelength accuracy: 2 nm or better;
Wavelength repeatability: 0.2 nm or better;
Bandwidth: < 2.5 nm;
Read-out range: Up to 4 Abs;
Stray Light: 0.05% @ 230nm
The instrument should have inbuilt incubation and linear shaking options for bio assays etc.
Incubation temperature: +4°C to +45°C.
Measurement speed should be 6 sec. for 96 well and 10 sec. for 384 well plate
System should able to run in stand-alone mode using 4.5 inch color display and keypad.
System should able to run with computer & software controlled mode.
The instrument should have a memory of 99 inbuilt protocols in stand-alone mode.
The instrument should have minimum 2 USB ports.
System should have Power Save function for reduced energy consumption when the instrument is „on‟ but not in use.
Instrument should have upgradation option for measuring accessory plate used for 16 low volume samples (2µl-10µl) in case of DNA/RNA estimation.
Instrument should have an option for path length corrections to correlate the micro plate data to cuvette, in case of nucleic acid quantification performed on micro plate
Software language should be English
Data Analysis Software Microplate based Spectrophotometer & Microdrop plate:
Analysis software should be supplied with the instrument and has unlimited user system license.
Software should have option for area selection. i. e different protocols at different area of the same plate.
Database based software to run backups of all data, restore back up data (in case of hardware failure of original computer).
Spectral scanning of all 96 samples or 384 samples should be able to view in single graph plot.
Single software program should allow any number of measurement steps within the program.
Software feature should include, calibration curve fit, parallel line analysis , user defined equations etc.
Parallel Line Analysis (PLA)
Improved Quality Control calculation
Improved User-equation calculation
Layout, protocol, calculations and reports should be edited freely
3. Required Specifications for ‘Mini Vertical gel apparatus, Western Blotting Module, Fast Blotting System and Power Supply apparatus’
Mini Vertical gel for 8 X 7 cm gels:
High throughput- Capable of running up to 4 mini gel (8 X 7 Cm) simultaneously.
Flexible- Capable of running hand cast as well as precast gel.
Running and casting module should be different.
Interchangeable module- Should be capable of using blotting module to do western blotting also.
Leak proof, tape free and easy assembly.
Patented Flap wing for leak proof assembly.
Permanently bonded spacer plates for leak proof, without agarose sealing & taping casting of gels.
System should have casting frame with simple cam closure mechanism that gives precision alignment on any flat surface.
Should have side by side casting stands that allow access to both gels simultaneously.
Modular design can be used do western blotting by using the blotting module only.
Should able to run gels in 15-20 mins.
Should come with buffer dam.
It should be Supplied with 10% Stainfree Fast Acrylamide Starter Kit.
Western Blotting Module:
It should be compatible with small SDS page Unit.
It should be capable of doing the western blotting of the mini gels.
It should be capable of running 2 blots simultaneously.
Color coded cassettes for proper orientation of gels and membranes.
System should be able to transfer two mini gels.
Ice free cooling unit for proper temperature control for western blotting.
Should be capable of using modular design to do electrophoresis by using the electrode assembly and caster only.
Fast Blotting System:
Should have following specs:
Fast blotting system with four gel simultaneously,2 different cassettes same protocol at different time in 3 mins.
System should be open for traditional blotting consumables
System should have inbuilt fix electrodes
Input power: 100–240 VAC, 276 VA, 50–60 Hz, 175 W max or better.
Should have USB port, input only, for firmware updates.
System should have Cooling fan
Should have inbuilt Power supply with high current of 2.5 A
User Interface
Should have 18 button keypad, 128 x 64 pixel monochrome display
Programmable methods: Up to 25 user-defined
Should have Preprogrammed methods: Standard SD, 1.5 mm gels, High MW, Low MW, Mixed MW
Audible alarm: Must be present.
User notifications should have following features:
Power fail during run
No-load detection
No cassette detection
End of run Power Supply apparatus:
Programmable power supply should be capable to operate four electrophoresis units simultaneously for four identical runs with graphic LED display.
The output range should be 10-300 V , 0.4-400 mA, 1-75 W .
Constant voltage, current or Power with Automatic crossover
Should have Memory storage: 9 programs , 9 steps, Timer Control : 99 hr, 59 min
Should have Automatic Power up after Power failure, Safety features: No-load detection; sudden load change detection.
4. Required Specifications for ‘Gel Documentation System’
Should be a Versatile system to support wide range of applications like- Fluorescent dye like syber green, syber safe, Western blotting, 2-D, 1-D, Dot Blotting, Nucleic acid detection , Densitometry etc.
Should have true 12 bit CCD camera with resolution Image resolution >4 mega pixels.
Should have Image acquisition with automatic focus and iris adjustments for all applications.
Should have Large trans illuminator area 25 X 26 cm.
It should universal dark hood & upgradeable to chemilumnescence.
It should be capable of Optimizing, saving, and quickly recalling the imaging acquisition settings.
One-time installation process should include dynamic flat fielding and flat-fielding.
Should have Safe DNA Imaging without UV exposure- using the Xcita Blue
Conversion screen to prevent damage from UV and preserve samples for downstream protein production.
Reproducibly position or center the sample on the image platen by using gel alignment templates.
Should have Stain free technology for faster imaging without any post gel electrophoresis staining.
Should come with 1 D analysis software with following feature
Automatic generation of customizable reports
Snapshot tool to copy images, lane profiles, and graphs
Complete flexibility with automatic and manual detection of lanes and bands, using several algorithms
Should have tri-plex image capture capability
Should have tri-plex image analysis features.
Easy copy/paste functionality, crop, zoom, 3D viewer, and colors.
Publishing resolution (dpi) and publishing dimension can be specified with a one-click image export for publication. Provides functionality to produce image at user-defined dpi and dimension
No requirement for license registration
Mac and PC compatible software
16-bit and 8-bit tiff images with a one-click export option.
The Software should automatically select the appropriate filters, light sources, and camera settings for all applications. Ensures that image optimization is specific to a selected gel or blot application or can be used for a large portfolio of detection methods. Applications including: Chemiluminescent,
colorimetric and multiplex fluorescent western blots, nucleic acid and protein detection via colorimetric and fluorescent stains.
Software should have automated multi-channel image view and color-coded analysis.
Software should have Signal Accumulation Mode (SAM) for easy optimization of exposure time for chemiluminescent detection.
Mac and PC compatible software .
Software should not have requirement for license registration allowing unlimited installations of the software for multiple users with full functionality.
Software should produce customizable reports with data organized as desired, including, Lane and band identification, molecular weight or base pair
evaluation. Band sizing and quantification are based on a reference band or quantity standards.
Automated normalization for loading control for total protein as a loading control.
Should be supplied with 2D software for spot matching and 2D gel analysis.
5. Required Specifications for ‘Specimen Freezing Microtome/Cryostat’
Should be a Freely standing cryostat with chamber temp. Down to -30°C .Cooling time (up to the max. low temp.) should not be more than 6 hours. Actively cooled quick freeze shelf with for freezing down to -35°C with Specimen storage shelf to store up to 10 chucks or more.
Should have Maintenance free microtome with section thickness setting preferably from 2 to 60 µm.
Equipment must be suitable for sectioning of specimen having diameter at least up to 50 mm or above & facility of Specimen orientation must be provided.
Should have Vertical Specimen Stroke length: 55 mm or above, Horizontal Specimen feed: Approx. 25 mm
Provision must be available for 2 Electric coarse feed(preferably at 900 µm/sec and 600 µm/sec. – as rapid & slow respectively) coarse feed speeds.
Should have Premium(High & low profile both) Disposable Blade holder system must be provided with lateral displacement and integrated glass anti-roll guide. Glass anti- roll guide with antistatic feature to facilitate of perfect stretching of sections
Should have Programmable chamber defrost facility must be available with at least 1 automatic defrost cycle/24 hours. Duration of the defrost cycle should not exceed 15 minutes.
Suppliers should have very good after sales service-support with proven track record.
o Supplier must ensure that the spare parts and consumables will be made available at least up to 7 years from the date of installation.
6. Required Specification for ‘Double-barrier Langmuir-Blodgett Normal Trough’
A. Double-barrier Langmuir-Blodgett (LB) Normal Trough
Normal Trough
Double Barrier
Trough & Barrier Fully made of High Purity PTFE
Symmetric Compression
Inner Working Area: 560 mm (L) X 200mm (W) X 5 mm (H) [22.05" (L) X 7.87"
(W) X 0.22" (H)]
Outer Trough Dimension: 590 mm (L) X230 mm (W) X 65 mm (H) [23.23" (L) X9.06" (W) X 2.56" (H)]
Deposition Well Dimension: 60 mm (L)X 200 mm (W) X 60 mm (H) [2.36" (L) X7.87" (W) X 2.36" (H)]
Trough Volume: 1220 ml B. LB System Control Unit
Fully Automatic & PC-controlled
Sensor Resolution: 0.01 mg
Surface Pressure Range: up to 80 mN/m
Surface Pressure Sensitivity: Better than 0.005 mN/m
Surface Tension Range: up to 110 N/m
Surface Tension Sensitivity: Better than0.5 mN/m
Measurement Accuracy: ±0.1 mN/m
Dipping & Lifting Speed: 0.5-250 mm/min
Dipping & Lifting Speed Resolution: 0.01 mm/min
Compression Speed: 0.5-250 mm/min
Compression Speed Resolution: 0.01mm/min
Universal Power Input
7. Required Specification for Chiller of High Pressure Equipment
Bath Temperature -20 to +100 oC
Cryogenic power at 20 oC 250 to 800 W
Volume of the vessel 3 to 6 L
Stainless steel tank
Tank vessel length x height ~ 400 mm x 150 mm
Chiller Height ~ 600 mm
Weight < 30 kg
7. Required Specification for Chiller of High Pressure Equipment
Bath Temperature -20 to +100 oC
Cryogenic power at 20 oC 250 to 800 W
Volume of the vessel 3 to 6 L
Stainless steel tank
Tank vessel length x height = 414 mm x 150 mm
Chiller Height = 616 mm
Weight 27.5 kg
8. Required Specification for ‘Lyophilyser (Freeze Dryer)’
Condenser volume: 2-3 Liters; Ice removal capacity: 1 Kg/24Hrs;
Max. Ice capacity: 1.5 – 2 Kg; Condenser Temp.: –500 C ± 5o C @ 200C ambient;
Compressor: Danfoss make Air cooled, 1/4 HP hermetically sealed
Outer top casing: Stainless Steel;
Direct driven high Vacuum Pump
No. of Stages: Two; Displacement: 100 L/min; Ultimate vacuum: 1 x 10 -3 mbar;
Oil sealed rotary vane type.
Low noise level
With built-in anti suck- back mechanism
Built in gas ballast
High water vapour handling capacity Motor 220V 50Hz, 1 Ph.
Oil mist eliminator: to keep the environment clean & pollution free.
8 - Port SS/Acrylic Drum-Manifold with ¾” quick seal valve, sealing gasket for freeze drying in flasks.
Rubber cone connectors for hooking Standard Round bottom Flasks with Standard B- 29 Joints.
Hand held ampoule sealing torch
9. Required Specifications for ‘Vacuum Pumping System’
A complete portable (mounted on a trolley) high vacuum pumping system with measuring electronics and display.
Ultimate Pressure: < 5x10-6 mbar
Pumping speed: The Diffusion pump should have a pumping speed of 125lit/sec or higher. It should be backed by a Rotary pump should have a pumping speed of 100 lit/min or higher.
Display: Vacuum and status (Digital indicator)
Drive motor: 220V A.C, 50 Hz Single phase power supply Venting mechanism: Integrated
10. Required Specifications for ‘Electrochemical Workstation’
Three (03) electrode configuration
Potential Range: ± 10 V at ± 250 mA or better Compliance voltage: ± 12 V or better
Maximum Output Current: ± 250 mA or better at ± 10 V Output Voltage Range: ± 10 V or better
Current Ranges: smallest current range: ± 10 nA to maximum: ± 250 mA or better Resolution of applied potential: 150 μV or better
Resolution of measured potential: 0.0015% measured range or better Accuracy of applied current: ± 0.2 % of the current range
Measured current resolution: 0.0015% of current range or better Potentiostat Rise/fall Time: < 1 μs
Applied potential accuracy: 1mV or better Bandwidth of electrometer: > 4 MHz or better
Fast data acquisition interface: USB interface for connection with PC Input bias current: < 20 pA or better
Input Impedance of electrometer: >100 GΩ or better Module for EIS Measurements
Hardware and software for EIS measurement over a wide frequency range of 10 μHz to 1 MHz. It should be supplied with powerful fit and simulation software for the analysis of impedance data. Data presentation: Nyquist, Bodn, Mott-Schottky, visually equivalent circuit input, automatic equivalent circuit parameters fitting. Data analysis: Fit and Simulation, Find circle, Element subtraction.
Electrochemical Software
Ready-to-use Vis & Generic interface for. Net applications should be included. Interactive visualization of impedance data; Laplacian smoothing, Delaunay triangulation, stereoscopic 3D anaglyph imaging. Several sets of data overlaid for comparison. several sets of data plotted side by side. Visually selected zoom area. x-y, ip-v, ip-v1/2, Ep-log v, semilog plots, linear polarization resistance plot. Simulation parameters: standard redox potentials, rate of electron transfer, transfer coefficient, concentration, diffusion coefficient, forward and reverse chemical reaction rate constants, temperature, electrode area, and experimental parameters. simultaneous display of voltammogram and concentration profiles. Voltammetry,
Square Wave Voltammetry. Electrochemical methods like Chrono-Amperometry, Chrono- Coulometry & Chrono-Potentiometry. Automatic detection and determination of over- determined equilibrium constants. Equilibrium data. EIS measurements. Software provision for photo-electrochemical measurements: I-V measurements, I max, Pmax, Fill factor etc.
Electrochemical Cell
It should consist of the following:
50 mL Glass cell 2 no, Pt disc as working electrode 1no, Pt wire Counter electrode 1 no, Ag/AgCl reference electrode 1 no.
Computer & Printer
A suitable branded Computer like Dell or equivalent for system control and data acquisition and a laserjet printer should be offered with the system. The computer configuration must be well compatible with the electrochemical work station and the software.
Techniques
Cyclic Voltammetry (CV)
Linear Sweep Voltammetry (LSV)
Staricase Voltammetry (SCV)
Chrono Amperometry (CA)
Chrono Coulometry (CC)
Differential Pulse Voltammetry(DPV)
Normal Pulse Voltamemtry(NPV)
Amperometric i-t Curve (i-t)
Differential Pulse Amperometry (DPA)
AC Impedance
Impedance – Time
Impedance – Potential (Mott-Schottky plot)
Chrono Potentiometry (CP)
Open Circuit Potential – Time
Impedance Simulator and fitting program
Bode and Nyquist plot option
Double Differential Pulse Amperometry (DDPA)
Triple Pulse Amperometry (TPA)
Integrated Pulse Amperometry Detection (IPAD)
Bulk Electrolysis with Coulometry (BE)
Hydrodynamic Modulation Voltammetry (HMV)
Sweep-Step Functions (SSF)
Multi-Potential Steps (STEP)
Galvanostat
Chrono Potentiometry
Chronopotentiometry with Current Ramp (CPCR)
Multi-Current Steps (ISTEP)
Potentiometric Stripping Analysis (PSA)
Electrochemical Noise Measurement (ECN)
RDE control (0-10V output)
Full version of CV simulator with fitting program
Impedance Simulator and fitting program
IR Compensation
External Potential Input
Auxiliary Signal Measurement Channel
Tafel Plot (TAFEL) Optional
There should be provision that in future the system will use in photo-electrochemical set-up/studies
System should be upgradable to Bipotentiostat in future
11.Required Specifications for ‘Minus 20°C DEEP FREEZER’
The Deep Freezer should deliver the sample protection, performance, security, and quality
• Integrated controller
• Digital display
• High and low temperature alarms
• Door ajar alarm
• Remote alarm contacts
• Standard door locks
• Standard casters
• Access ports
• Low energy consumption
• Solid door
• Forced air circulation
• Automatic defrost
• CE marked
• Reversible doors (400 Liters module only)
• HCFC- and CFC-free insulation
• Interior fluorescent lighting
The lab freezers should be fitted with standard door locks for peace-of-mind.
The lab Freezers should have integrated digital controller with temperature display, door ajar alarm, and high/low temperature alarms is easy-to-use and maintains the correct cabinet temp.
The lab freezers should come standard with access ports making integration of secondary monitoring systems simple.
Technical Specifications
Temperature Range: -10°C to -25°C
Capacity: 400 Liter;
Defrost Type: automatic
Door Type: solid
Includes: 3 or more shelves
Internal Dimensions (W x D x H): Minimum 480 x 500 x 1150 mm
External Dimensions (W x D x H): Maximum 600 x 650 x 1850 mm
Voltage: 230V/50 Hz
Plug Type: EU
General Instructions and Terms and Conditions:
1. Incomplete & conditional tenders and tenders received after the due date will be summarily rejected without assigning any reasons thereof.
2. Only experienced suppliers (sole manufacturers or authorized distributors) should submit quotation. The submitted quotation should accompany valid documentary evidence that relates to the experience of the company in related field for more than 15 years.
3. The Supplier/Manufacturers/exclusive distributors/vendors should have history of supplying, at least ten, this type of instrument to this or other University/Research Organizations/Institutes.
4. Please attach the list of similar installations done in India.
5. The quotation should be clearly written and the specifications of the quoted item should be properly mentioned, which should follow the points mentioned under specifications of the requested item. The model number, make, and a printed literature of the product shall submit positively.
6. Payment: 100% against delivery and successful installation subject to submission of performance guarantee.
7. Installation/Demonstration /Application Training at site: Free of cost by the supplier
8. Warranty: Minimum 02 years
9. Service facility: Supplier should mention their details of service setup and Manpower at Calcutta who will be responsible for after sales support. Response time should be within 48 hours.
10. A non-refundable charge of Rs. one thousand only (Rs. 1000/-) is applicable with the submission of the tender documents. This should be paid by demand draft payable in Kolkata, India, drawn in favour of “CRNN, University of Calcutta”.
11. If the quotation is being submitted on behalf of a principal company, a valid authorization certificate of dealership should be submitted. The dealer should also provide a certificate stating the permission to quote the product under consideration to CRNN, University of Calcutta. Any photocopies submitted in this regard should be self attested with company stamp.
12. The company should provide valid documents related to the permanent account number, sales tax, value added tax and trade license (for the current financial year 2014-2015), with self attested photocopies and company stamp in the documents.
13. Price should be mentioned in Indian currency as well as in international currency (wherever applicable) and should include all other charges (carriage, insurance etc.) until it reaches the destination, including taxes. Installation charges (if any) should be included within the quoted price.
14. Delivery and installation time frames should be properly mentioned in the quote. The instrument should be delivered within thirty days from the date of placing the order.
15. The product under interest will be used for educational purposes. Any educational discount should be included and clearly mentioned in the quotation.
16. Quotes should be valid for at least four months.
17. The purchasing authority reserves the provision of minor modifications in the specifications, only if it feels as an absolute necessity.
18. Guarantee certificate, users manuals etc. are to be handed over to the user after successful commissioning of the system.
19. In case of any dispute, the decision of the Institute authority shall be final and binding on the bidders.
20. The university reserves the right to accept or reject any or all the submitted quotations without assigning any reason whatsoever.
For further any queries, please contact Dr. Prasun Mukherjee, Dr. Arghya Adhikary, Dr.
Gobinda Gopal Khan, Dr. Jayanta Kumar Bal and Dr. Prasun Patra at CRNN, University of Calcutta on any working day and normal office hours before the submission of the tender documents.
