NARAJOLE RAJ COLLEGE

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NARAJOLE RAJ COLLEGE

(NAAC Accredited ‘B’ Grade Govt.-Aided College)

NARAJOLE: PASCHIM MEDINIPUR: PIN-721211

Ph.: 9475429270 e-mail: [email protected]

BOTANY DEPARTMENT SEMESTER VI (Honours)

PAPER DSE 4T (Analytical Techniques in Plant Science)

(Unit: 1, 4, 5 and 6)Teacher Name: Dr. Prithwi Ghosh A. Each question carries two marks. (2×15)

1. What is m/z?

2. Name two fluorescent dye.

3. What is cryofixation?

4. What is resolving power of a microscope?

5. How to detect proteins in SDS PAGE?

6. What is the principle of electrophoresis?

7. Name two aromatic amino acids.

8. What is peptide bond?

9. What is FISH?

10. Name two reducing agent used in protein gel electrophoresis and mention their function.

11. How do you visualize your DNA sample in agarose gel electrophoresis?

12. What is mobile and stationary phase in chromatography.

13. Why is the resolution of an electron microscope greater?

14. What is the full form MALDI-TOF?

15. Name the dye used to visualize sample in SDS-PAGE?

B. Each question carries four marks. (4×5)

1. Write notes on: Negative staining and shadow casting in Electron microscopy.

2. Write down the difference between SEM and TEM.

3. Suppose you are running a dimeric protein in both native PAGE and SDS-PAGE. How the result will vary and why? Explain with diagram.

4. Explain the principle of affinity chromatography.

5. What is flowcytometry? Write down the application of flow cytometry.

C. Each question carries six marks. (6×6)

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1. With labelled diagram write down the principle of Confocal microscopy. How it is different from fluorescent microscopy?

2. What is MASS SPEC? Explain its working principle with diagram.

3. What is chromosome banding? Discuss its types.

4. What is Spectrophotometry? Discussing the principle of Spectrophotometry by explaining the Beer-Lambert Law.

5. Give the principle & ray diagram of SEM or TEM.

6. Name the technique you will use to study the following:

I. Protein Structure.

II. Molecular weight of protein.

III. Peptide fingerprint.

IV. Protein concentration in a given buffer.

V. Localization of a protein (transcription factor) within the nucleus.

VI. Purifying His-tagged recombinant protein fromcell lysate.

(Unit: 2 and 3) Teacher Name: ProfessorBangamoti Hansda

A. Each question carries two marks (2×10)

1. What is cell fractionation?

2. What is the principle of centrifugation and write its applications?

3. What is marker enzyme?

4. Who discovered cell fractionation technique?

5. What is cell homogenization?

6. What are the types of centrifugation?

7. What are isotopes and radioisotopes?

8. What are the examples of radioisotopes?

9. What does the term pulse refer to in a pulse-chase experiment?

10. Who did the pulse-chase experiment?

B. Each question carries four marks (4×5)

1. What is differential centrifugation?

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2. What is density gradient centrifugation?

3. What is sucrose density gradient centrifugation?

4. What is CsCl2 gradient centrifugation?

5. What is the principle of autoradiography and what substance is used during autoradiography? (2+2) C. Each question carries six marks (6×5)

1. Describe the steps involved in cell fractionation process?

2. What is analytical ultracentrifugation?

3. How does a pulse chase experiment work?

4. What are the marker enzymes fore different organelles?

5. What are the uses of radioisotopes in biological research?

Unit: 7 (Teacher Name: Professor Sanjay Kumar Datta)

1. Mention the properties of normal probability curve. /distribution curve.

2. What do you mean by Sample Data,Variance, Standard Deviation.

3. What do you mean by dispersion or variability.

4. What is skewness and kurtosis.

5 What is test of significance.

6. What is CHI - SQUARE test and Goodness of fit.

7. Classify the data and calculate Mean and standard deviation.

No of seeds/fruit

12 14 13 6 7 21 17 15 13 12 7 8 11 20 11 19 18 9 10 13 13 15 12 14 13 11 9 8 16 14.