, ) (
*
:
:
E-mail: m.rastgou@urmia.ac.ir
هرﺎﭘ ﻲﺳرﺮﺑ ا
ي ﺻﻮﺼﺧ زا ﻴ
ﺑ تﺎ ﻴ ژﻮﻟﻮ ﻳ ﻜ ﻲ ﻣورا هﺰﺑﺮﺧ سوﺮﻳو ﻴ
ﻪ
(Ourmia melon virus, OuMV)
، نآ عﻮﻗو رد
ﺪﻨﭼ نﺎﺘﺳا ناﺮﻳا و
زرا ﻳ ﺑﺎ ﻲ ﺶﻨﻛاو ﻠﺤﻣ مﺎﻗرا
ﻲ رﺎﺠﺗ و ي ﺧ و وﺪﻛ ﻴ
و ﻪﺑ ﺖﺒﺴﻧ رﺎ ﻳ
سوﺮ
ﻮﮕﺘﺳار ﺎﻨﻴﻣ
*1
ﻲﺒﻴﺒﺣ ﻲﻫﻮﻛ ﺎﻨﻴﻣ ، هﺎﻨﭘدﺰﻳا ﻪﻟا ﺖﻣاﺮﻛ ،2
،3
ﻲﺒﺣﺎﺼﻣ ﻦﻴﺴﺤﻣﻼﻏ ﻲﺟ تﺮﺑار ،4
5ﻦﻠﻴﻣ ﺎﻨﻳرﻮﺗ ﻮﻤﻴﺳﺎﻣ و
6
!
"
#$
%
& '(()$ * + ,- ./ -!+
0 1 2 '(()$ * ,- .
3, 45$ ./ -
)
$ 78 9 :
; /
= /
;;
>
?5@$ 9$
: A / /
;B (
هﺪﻴﻜﭼ
)
Ourmia melon virus, OuMV
(
)
Cucumis melo
(
!
"#$
$%
!
&
! '*
.
%%
+ , +
!
! -/0 '1 2 -/0 '
34
5 6 +
78 9
!
Indirect-ELISA
،
DAS-ELISA
Lateral flow
! ':;,<
. ,':1=
!
! 4
!
>
+
!
?@
A
?B
%% 2<
5 + 'C '*
; DE D%
FE G '*
.
!% H4 ! I
+, '1
I I
I I%':+ IJ1
8 IK:L
+ I , IM
&%;
!
%
'FE
!
=
N I O$ PE
! QL , :* G1
! '*
. +
I'* R% >S%
4 D:E
; E T
9
! , '* S: -8 8 + .
! UE V S W=
R<
I U R<
!
S '1
E R< X
X$
V O '% O%
. 2<
&% D%
HY ZS%
!
* ' .
هژاو ﺎﻫ ي يﺪﻴﻠﻛ :
،
DAS-ELISA
،
Lateral flow
، R< V S
U
! I
.
ﻪﻣﺪﻘﻣ
! "! # $%
&
% '( )*$
+ ,' -
'( )*$ +
% ,'
&
!
&
+ +$
. /
! . 0
1 )
Torino, Italy
( 0 4567
08
&
-
9+*
9; "9<= % >
'? -1 + @
&
A$B C) ; +
DE - +F
&
-% + G 0$H )*$
(Lisa et
al., 1988)
. + + )%$J #+$
-
(Ourmia melon
virus, OuMV)
0 0;
; - KL -
$%
. G
!M G )
Caciagli et al., 1989
.(
; NOJ ,
% $O(
- + 0$H #+$
.
>
-%
P QG
$
!
&
RS
% . T >+% QG
&
0$/
% T +0
&
+;
-
U S + 0
$VW -% ) 46
B % + $
!
&
LX Y
Z / KZ Y
\7 +
\ $ 0$* G
(Lisa et al., 1988;
Marzachi et al., 1992; Milne, 2005)
. ]$% 0 &!
T U;
; G -
^H%
!
&
P 01F
$
!
&
+ 0 #+$
"<P
!
&
MP
!/ + "
. ,O% ,W+
!J
&
pad-like
0
% QJ0 G Q%W !
.
! )
Milne & Masenga, 1988; 1989
.(
_`
+ -8VW - QG #+$
F .
>
.
&
@ a
&
J V . ^Sb
11 >;+ -%
. 4
× 54 / >10 )
(YRNA1
4
×
\Z / >10 ) (RNA2
+ 4
×
\X / >10
) (RNA3
. G%
. +$P MGP .
+ >;+ -% #+$
11 . Z kDa
/ XZ
^ . @!<* 0E+
) (cap
+
VPg
T 0
&
' Z P -1S0 .
F )
poly A tail
( T 0
&
'\
^ M deM )
Milne, 2005
.(
* A0$
&
_` J + +
0 #+$
Xff5 G 8
) Rastgou et al., 2009
.(
` ,8VW ; .
+ #+$
+ )%$J
-Y + #+$
g hE Y % -
$ i X64K Y
4fLK + 57K
* 8
$! -* G ; _*
0 ,8VW
&
;% jJ kll @ .
G%
.
+ RNA1
#+$
Y +$P Z / 57 * 10 . *
. +? -* *
&
m F -OeMGDD
.
>
.n P ;$
F -% -%+
.
>
.n
(RNA dependent RNA polymerase,
RdRp)
+
#+$
!
&
SVW % a @
^Sb ^ .
G%
.
+ RNA2
#+$
Y +$P L/
\4 * 10 . *
. +$P -* *
*$?
. + #+$
^ . O(
+ORF3
#+$
+$P @ 6 / X\
* 10 . -* ^
+$P )
MGP . +
^ #+$
(Rastgou
et al., 2009)
. -0
%) . SB 8 . + #+$
>*
1+ ^ M deM .
+ F -% #+$
. -0 -%
+ 8 . / ; B -% @( >!
. ' Q%W
0 + 0%
OeM "9<= TF $b*
.
^ 0$* 0o .
+ #+$
\K ; -/
4K / 0J
! . 01F .
*
)
Lisa et al., 1988; 1990
.(
+ + )%$J #+$
$! -
+ % #+$
. +*
)
Cassava virus C, CsVC
( +
+ P #+$
/ #+$
#<
)
Epirus cherry virus, EpCV
(
hE 0
Ourmiavirus
$W 00
(Accotto et al.,
1990)
. /0; -% -E %
+ + #+$
,=<B 0S )
H*
. + 0 >F % -V% 0
+ - > p' $
%qF
>o
%$r . + 0E+ ])/ ) +
> 0 #+$
/ ><
)
Gholamalizadeh et al., 2008
( + .$% _=
0 #+$ + 0E+ 0 0 s'( + >$ $ 0 sB
+ 0 sB 0 >F 0E+ + ]$/ ? $
; A!
_o '(
s
% Y - %0 _;a ,=<B >0+F ^0
%
`1
&
+ -0 -E ; #+$
%) . SB 8 . Y
+ M sB Y'
Q( >) ) ^+' + &$ tP
^Mu 0 _W -% ^S
+ #+$
-'V 0
. % G .
شور و داﻮﻣ
ﺎﻫ
!"
.B &!
.=;
67 6Z C) ; +*
>
> me sB
%qF
%$r >o . Q= -% 0;%
F . / 0 >!
&
<=
; "
@ DE G &+F
+
-% ' ; hP
;F M .F ; 0 %
&0%
!
&
-!l &
) .P u ( + + )%$J #+$
-
)
Ourmia melon virus, OuMV
(Y +
; #+$
0; @
+*
)
Zucchini yellow mosaic virus, ZYMV
(Y
+
; #+$
-+! @
(Watermelon mosaic virus,
Y WMV)
+
; #+$
J @
(Cucumber mosaic
virus, CMV)
Y + '? -1 #+$
&
PP
(Papaya ring
spot virus, PRSV)
+ + +* 0; -1 #+$
(Zucchini
yellow fleck virus, ZYFV)
% 1 ]+ ; 0
)
&
r ' $ "
) Indirect-ELISA
( + + 0 0
_+0 % ]+ +0 "' &)1
(DAS-ELISA)
+ >;F
E ^M S . )
Lateral flow
(
$W .% ; 0 H$/
.
SE ^M >;F 0 .
F . 0%
&
F + . 0%
&
-% QO
)F BVJ ,O% "
. +
&
!
&
)1+$
&
$W
. /
$ . 0 / O= >;F
"1 + 01F >!
+
&
$W ! .
/ 0$
. / -% p%$ 0
"1
T + % @ / -% p%$ 0
% +0 01F
!M .
0G . F _ . 0%
&
F + ! .
0%
&
!
&
)F -% QO 0 G 0 "
'(
)*$ 0 s
'(
+ ,' G #+$
. 1 T G - .
0
$% @ . ' . 08 Z A$B ; +* -
G + ;$
Z +* - Y
Z - )%$J Y
\ + ;v* -
4 J - '( -=) ;
' .
;+M* M0
&
w$*
) a ]+ % )
&
; 0 "' %
.
H$/$W .
! #$
$ %&'
( )*+
8 &$%
>%)
!
#+$ + .=;$r &
&08 Y ;
m=
!
&
+* C) ;$!
01F >
-%
+
#+$
DE G &+F
.
>%) 8 &$%
Y#+$ + .=; &!
- C) ; &0$%
+* me Y
J Y )%$J Y
;v*
Y
+ -+!
-E/ )
$H . Y i
; . Y
>o0%
Y
! x
%1 + 0 + A$B
-
^H$/ ,N .
yu -%
>;F &!
DAS-ELISA
+
Lateral flow
./01F
-
! -%
^H$/$W .$% 0 #+$ + .
,",- ./"
^TE 8 + ' ( TG+ #+$
&
' ; $
^H$/$W -81V 0 :
+ 0 ./"
1 2
!
-e/ 0 Z
>/
; / >!
-
; . % G
+ + )%$J #+$
+ - Z ; >/
/ J "1 >!
Y
)%$J +
N. benthamiana
/)% A$z QJ0 0 .
$W
H$/
.
$%
&
/E
$
&
+ ' _= ;
$B ; #+$
s
#
% 0 D @
/ >!
-
; . "1 + G
G 00$W .
/
%F >!
&
$B ; T + G kF s
%F
&
P # "! %
0$*
. $ - 0 >;F
G _o .
$%
&
8 ; '
>;F
!
&
`1+$
.
DAS-ELISA
+
Lateral flow
G 0 .
+ 0 ./"
3 2
&
4
Z +? >/
&
/ >!
-
; . / + G "1 >!
J Y + )%$J
N. benthamiana
/)% A$z QJ0 0 .
B
&
/ -* H$/ $W
>!
-
; . / % G >!
$W # 0 "1
%
$ . _o $ - 0 >;F
G .
$% QSW ]+ s%V
&
8
>;F ; '
!
&
`1+$
.
DAS-ELISA
+
Lateral flow
G 0 .
+ 0 ./"
5' 2
(6
/ >!
J Y + )%$J
N. benthamiana
QJ0 0
/)% A$z .
G ^M* "! * 0 .
/ $! ; Xf t%
G -G*
. G _o $ - 0 >;F .
; 8%
@
/ -* -!
H* ; -% >!
. 0$* G 4f
-%
; /
+ % >!
+ )%$J #+$
- -
; . G . @
; 8%
-
; .
$%
&
8
$B ; ' + UJ s
# -M ; !
>;F
!
&
`1+$
.
DAS-ELISA
+
Lateral flow
G 0 .
+ 0 ./"
%7 &$ 5' 2
$%
&
$%
.
$B ; ' ? UJ YUJ s
0$% - 01F C)
&
-% ' ; hP + G
;F M ' ^W +0 -% UJ
"
G . @
^W ; 0 %
$ +<*
Q G 0 ^W + $
! >+% >F
{
= -/
. 0 0
^H$/ $W .
4f
-
$ UJ r + Q
$$
Q $! 0 + G 0F
- 4f
* "1 t%
G - G . ^Gt/ ; 8%
+0 + @
je% ; - ^G0$% -% _W
!
&
a%
. + )
/ -M 0$/ >!
. /01F ? .
>;F %
`1+$
.
DAS-ELISA
$% 0 .
^H$/ $W .
+ 0 ./"
%89 2
: 5' ( %7 %
/ / O= ,H $H% % 01F
$
&
O= + G
P 0 01F
&
/ J "1 >!
Y + )%$J
N. benthamiana
G -e .
$v ; 8%
&
>G @
$B ; ' Y
`1+$ >;F s
.
DAS-ELISA
$% 0 .
^H$/$W .
0 ;<
:=$ >
! ( ?$
$ %&'
(
$%
&
( _W | .
J + -+! Y)%$J Y
o _W + +*
&
+ ^M* -e/ 0 0E @
; 8%
-
; . + } + )%$J #+$
Y- >)
? Q( + ^ tP
$
&
_W %
`1+$ >;F
.
DAS-ELISA
"9<= !M + .$%
G .
$%
&
"W $!
QW?
7 >/
>/ $! 0 + L
-G* t%
G .
o _W
&
; G 0 $ :
_W J :
cucumber Marketer
+
cucumber Apollo
.
)%$J _W :
melone FA supermariteet melone
Vedramtais melone Meles Best Jumbo
smguris
crimson sweet
+
smguris sugar baby
.
+* _W :
squash COMG
+
zuccini CEMOUSE
.
ﺎﺘﻧ ﻳ ﺚﺤﺑ و ﺞ
$ %&'
(
@:A$ 2+$
-
! &
0
&
'? -1 "9<=
&
+
; ) @
) QG 4 ( +* me C) ;
%qF > >
>o
%$r . DE +F
&
>;F ; 0 % + !
&
`1+$
.
Indirect-ELISA
=; 0 .
6L 6Z +
DAS- ELISA
+
Lateral flow
=; 0 .
67 6L 0
$%
. H$/$W .
08
&
; -
! -e/ 0
+
&
J -E/ + +* Y
$H . -
; . G ) QG X .(
x
$%
. +E 0
&!
4 + X G -N<J
.
+
$J #+$
+ )%
B - . 1 +0 .
>!/ 0
J + +*
N ,aO( -*
. > 0 G ^M*
. G%
eM G 00 d .
+ 0 #+$
C) +*
0
3 2 1
45 . %, & - .67 ( 89;<
<
=> %? @A . "# .
A . C $ . %, & D#E 4 89;< - ,% 3 B 1 F 8G , . .67 ( 89;<
H . I- 89;< B
F 8G !J
C
F 8G .45 . I- .67 ( 89;< D
K4H 2 3 45 L (?
"#
%M?
%N % %, &
<- K #
OP 3 OQ
(%)
(%)
!!
/
!!
" #" '"&$/#$ "!
*
+ ! "* #&$ ,-.
+
+ + '0/&$ !&1
"*
# ' '* #*' 2
"*
# ' !3/&0 #!" 4 + +
+ + #0/$ # 50 867 + 3#
/
!#
$ '' &1!$/01 #!*
K4H B 3 45 L (?
%M? "#
%N % %, &
<- K #
OR 3 OP
(%)
(%)
!!
/
"3
#& '& #*#0/& 0#
*
+ ! $/$ #!# ,-.
+
+ + /0$ #"#
*
+ ' !!/!! # 2!
*
+ " #0/$ # 50
'&
/
&'
#& !! #&#&/'3 13
' J % -
% M
$M
&
^G0 .
~!
./01F
-% C) +
=; 0 #+$
. 6L 6Z % ; $M
=;
. 67 6L 0%
.
; Z +* -
&
DE +F
&
G A$B ; G
+ ;$
; Z +* -
&
DE +F
&
'( -=) ; G
' .
$! w$* A$B _*
\ ; Y01F - Z
)%$J -
; + - @
\ ;v* 0 X
; + - - @
J >!
+ -% 01F - @ eM #+$
00 d
G .
% - $|
. + + )%$J #+$
-'V 0 -
%qF
>o
%$r . + 0 ) p' $ > -E ; >$
/ ><
)
Gholamalizadeh et al., 2008
( + ;
#+$
!
&
01F "T +* *
>
G%
. G . ju$P +
+ B #+$
.
!
&
=;
. 67 6Z 0 +* C) >
>
_o G .
=; 0 .
_+0 -% C) ./01F
+ ' 0 #+$
=; + % - .
$*
0%
. .
a0 ; Q . 1MJ .
V 0 G% -' . ; $
8% 0 >;+M*
.
; V sB j!* ^M* $
0 sB 0 + 0% 00 ^G* ; $
/ >!
00J
&
0% 0$*
.
$%%
; _+0 0
0$% - sB
&
Q8%
. + + ; #+$
&
/ >!
=;
. +* $ G ])/ >
. $ ; V -% -E %
+u ^Mu 0 + _' > 0 -u
. G%
./01F
0% > +u $ ; $M% #+$ + -% +u C) .
$ ($
"&
*+
90 90
$ %&'
(
-
! &
0
&
/ "9<= >+% + "9<=
=; >!
.
r +
=;$
. 9+* C) ;
+ A$B >
/01F -* - .
C) + -%
+ )%$J #+$
>;F % -
!
&
`1+$
. 0% G ;$( TF 0 DE
+F
&
% +
; 0
>;F
!
&
`1+$
.
Indirect-ELISA
0
=;
. 6L +
DAS-ELISA
+
Lateral flow
0
=;
. 67
$% 0 .
$W H$/
.
% 0
>%)
!
&
=;
. Y + $ J + +* $% +<= #+
;
/ $ >!
9+* $ )%$J >
(Cucumis
melo)
J Y $Sl
(C. melo var. flexuosus)
;v* +
)
C.melo var. dudaim
( G . G ) QG
\ .(
+
=; 0 #+$
. 67 + ; T
&
- @
-+!
)
Citrollus vulgaris
( 0
&
9; "9<=
@
eM G 00 d .
$% +<=
+ + #+$
&
/ >!
o0% $
+ 0 >
&
$H -E/
.
(Lycopersicum
esculentum Mill.)
>o0% +
(Solanum melongena
L.)
0 ) % . 0$/
% 0 / $
=; >!
.
! Y x
)
Dacus carota
(
%1 +
(Phaseolus vulgaris L.)
)
+ -%
01F #+$
eM G 00 d )
QG K (.
/ >!
QH )
Capsicum annuum L.
Y(
i
; .
(Solanum
tuberosum L.)
P Y ;
Allium cepa L.) (
!*
(Lactuca
sativa L.)
Wl
(Beta vulgaris L.)
>0$%HF +
(Helianthus annuus L.)
-%
+ /01F #+$
.
G ) QG Z .(
% 0
%) r >
=;$
.
$%
.
+ YG #+$
0 P @~
)
Convolvulus sp.
#+$J w (
M?+
. )
Amaranthus retroflexus L.
( * l+/ +
(Lactuca scariola L.)
G . G )
QG L (.
$G
)
Echinops bicolor L.
( -SP+/ Y
(Abutilon
theophrasti Medicus)
$GJ Y
(Alhaji camelorum
Fisch.)
@ Y )
Chenopodium quinoa
( - Y $
)
C. album
( _/ Y )
Sorghum sp.
(
$E Y )
&
(Solanum nigrum L.)
G +
$
% >
(Glycyrhiza
glabra L.)
+ -%
/01F #+$
. 0 >M .
0 x
+E
!
&
\ + K
^ G -N<J .
+ R<SW #+$
+ ;
&
SB 0 )%$J + +*
^8
G . 0% G )
Lisa et al., 1988, 1990
( 1+
. 0
$%
. +
$% #+$
&
1+
+ ; %
&
Y;v*
J Y-+!
%1 Y$Sl
! Y
$H -E/ Yx .
>o0% +
>= -%
>%)
!
&
SB 8 . + + #+$
08 )
&
r >%)
=;$
. P -E ;
@~
Y M?+ #+$J w .
+
l +/
G * .
0$/
. + Ra? #+$
+
&
/ r >!
=;$
. P Y#+$J w -E ;
l+/ + @~
_+0 * .
0+F . 0 '(
/ s - >!
+ $ + -% 01F @
eM #+$
M 00 d .
l$!
+
% #+$
-
;F B
!M . / 01F >!
.
*
)
Lisa et al., 1988, 1990
( . 0 ? -S1 08 0
-
!
&
$%
.
$% G
&
; % . T . G% "*
.
+
% #+$
=) 0 $M .
G . l -* G C
/
* "! * 0
G - G 0%
. R<b J Y+*
Y
$H -E/ Y;v*
.
%1 Y V 0 >o0% + QH Y
0 "! * 0 "*
G ^M* -=) @ 0%
.
~!
+ $ + ; w$* A$B ; #+
&
J Y+*
Y
+ )%$J + ;v*
G A$B ; )
+ ; ;$
&
+*
G . G .
1 S 3 I- 89;< % -T5 A B
% (? 5 4% I- 89;< % , 44 UCVE %, &
.
1 W 3 XE .% 5 $ - ,%
A Y
Z? - %, & % .%
[J )*
: I- 89;< % 1\J A
%( B
I- 89;< % .- 89;< % @$ C
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REFERENCES
1. Accotto, G. P., Riccioni, L., Barba, M., Lisa, V. & Boccardo, G. (1990). Ourmia melon and epirus cherry viruses, two representatives of a new virus group. In: Proceedings of the 8th International Congress of Virology, Aug. 26-31. Berlin, Germany. p. 448.
2. Caciagli, P., Luisoni, E., Vecchiatti, M., Lisa, V. & Parvizi, R. (1989). Ourmia melon virus: update on its biology. In: Proceedings of 6th Conference, Recent Advances in Vegetable Virus Research, Aug. 27-31, Asilomar, California, USA. p. 9.
3. Gholamalizadeh, R., Vahdat, A., Hossein-Nia, S. V., Elahinia, A. & Bananej, K. (2008). Occurrence of Ourmia melon virus in Guilan province of Northern Iran. Plant Disease, 92, 1135.
4. Lisa, V., Milne, R. G. & Parvizi, R. (1990). Ourmia melon virus and other cucurbit viruses in West Azerbaijan, Iran. FAO Plant Protection Bulletins, 38, 218-219.
5. Lisa, V., Milne, R. G., Accotto, G. P., Boccardo, G., Caciagli, P. & Parvizi, R. (1988). Ourmia melon virus, a virus from Iran with novel properties. Annals of Applied Biology, 112, 291-302.
6. Marzachi, C., Antoniazzi, S., d´ Aquilio, M. A. & Boccardo, G. (1992). Molecular cloning of the segmented RNA genome of Ourmia melon virus. Journal of Plant Pathology, 2, 3-8.
7. Milne, R. G. (2005). Genus Ourmiavirus, In: Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., Ball, L.A.(Eds.), Virus taxonomy, Eighth Report of the International Committee on Taxonomy of Viruses. United Kingdom. Elsevier/Academic Press, London. pp, 1059-1061.
8. Milne, R. G. & Masenga, V. (1988). Particle structure of Ourmia melon virus, a virus from Iran representing a new taxonomic group. In: Proceedings of International Symposium of Plant Pathology, Sep. 1-5. Beijing, China, p. 549.
9. Milne, R. G. & Masenga, V. (1989). Structure and cytopathology of Ourmia melon virus, a virus from Iran with novel properties. In: Proceedings of the 6th conference, Recent Advances in Vegetable Virus Research, Aug. 27-31, Asilomar, California, USA, p. 21.
10. Rastgou, M., Koohi Habibi, M., Izadpanah, K., Luisoni, E., Masenga, V., Milne, R. G., Wolf, Y. I.,
Koonin, E. V. & Turina, M. (2009). Molecular characterization of the plant virus genus Ourmiavirus and evidence of inter-kingdom reassortment of viral genome segments as its possible route of origin.Journal
of General Virology, 90, 2525–2535.
