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بررسی اثر فرمولاسیون‌های پودری جدایهBacillus subtilis M36 در کنترل Fusarium solani f. sp. phaseoli عامل پوسیدگی ریشه لوبیا

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ﻻﻮﻣﺮﻓ ﺮﺛا ﻲﺳرﺮﺑ نﻮﻴﺳ

ﺎﻫ ردﻮﭘ ي ي

اﺪﺟ ﻳ ﻪ

Bacillus subtilis M36

لﺮﺘﻨﻛ رد

Fusarium solani f. sp. phaseoli

ﺳﻮﭘ ﻞﻣﺎﻋ ﻴ

ﮔﺪ ﻲ ر ﻳ ﺎﻴﺑﻮﻟ ﻪﺸ

ﻲﻤﻗ ﻲﺋازﺮﻴﻣ ﺎﺴﻳﺮﭘ

، 1

ﻲﻧﺎﻣز ﺎﺿرﺪﻴﻤﺣ

2هداز

، يﺮﺑﺎﺻ ﻪﻟا حور

3ﻪﺴﻳر و ﻚﻟ ﺎﺿرﺪﻤﺤﻣ

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Fusarium solani f. sp. phaseoli

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هژاو ﺎﻫ ي ﻠﻛ ﻴ ﺪ ي :

Fusarium solani f. sp. phaseoli

7J2' 34 7' 7

Bacillus subtilis

IF 7 .

ﻪﻣﺪﻘﻣ

Fusarium solani f. sp. phaseoli

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0

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I #-=( %

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72

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1997)

.

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0 (

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.

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/

( )

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Traquair, 2002)

@

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^ _ (

O8(

O(%C %:() K

( \ B:$

˚C

`a

_ (

(Bora et al., 2004; Sabaratnam &

Traquair, 2002)

.

%C O(

K ) b!

Bacillus subtilis

%:B\

Rhizoctonia solani

/2( ) V 2\ c d%( "(/

%FP(

(Sharifi-Tehrani et al., 2007)

. O(%C K

b!

ef ) ghf

Pseudomonas putida

B\ /2( ) V D%

%(i %:

*2% (C

%FP(

(Bora et al., 2004)

.

O(%C K ) b!

Pseudomonas

fluorescens

%:B\

Rhizoctonia solani

"(/

) V 2\ c d%(

/2(

%FP(

(Sharifi-tehrani et al., 2007)

. )%C K%

Phyllantus amarus

O(%C K N!

(BSCEB4)

B. subtilis

)

P. chlororaphis

#(

ef

@4

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(Mathiuazhagan et al.,

2004)

. <

4'! 0 : L

%

%:

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'(

<%> D

% )

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(H D ) *_

V

% "(/

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شور و داﻮﻣ

ﺎﻫ

Fusarium solani f. sp. phaseoli

%

F. solani f. sp. phaseoli

D!

Noori (2008)

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0 ) K:

2%(

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Shahrokhi (2008)

% C .

! F. solani f. sp. phaseoli "#

$

%

%

% #

%

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S)

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(1989)

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1 v:

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(2001)

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(2001)

7E .

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S) O! K(H

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@ K(H

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(2001)

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.

(4)

&

75 6 Bacillus

subtilis

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S)

Bora et al.

(2004)

%

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;

5< (5

F. solani f. sp. phaseoli

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Spigel & Hall

(1982)

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K)

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= g

A (

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€ (

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% ! : KO(%C S) %

Bora et al.

(2004)

*?:

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( )%=

%:

ef :/

) (

`h v$

(

B. subtilis M36

% '( N D

@ M1

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KB

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l }V( + -( ) % C~

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2.Glyserol 3.Sodium alginate 4.Vortex 5.Talc

(5)

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%:

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Z t

gf

%

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@ h )

‹

$Z N! K

B. subtilis M36

D'( )

{ƒ( H 4(

gff (

%=

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%:

! % : %: N

(

* + ) JV @

=

!

(

`h v$

! C% %

.

% %=!

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* S KO(%C 7% %:

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‹ 8( )

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6'( H .

=&' -

"

A!

B. subtilis M36

B+

F. solani f. sp. phaseoli (5

O(%C t K

B. s M36

@

S) L6>

Sabartanam & Traquair (2002)

8!

0

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2.Xantan Gum 3.Arabic Gum 4.McCarteny

8

%:

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7E

4( )

O(%C %

)R K

gf

%:

6'(

) ( 0 K2 7% %

{ 0

% :

M1-CMC = 0.16g NBY-AG = 0.62g M1-AG = 0.1g NBY-XG = 0.62g KB-XG = 0.25g NBY-CMC = 0.62g KB-AG = 0.2g KB-CMC = 0.31g M1-XG =0.12g

) :V %: K) "1R %4( N % ) ){

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.

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%

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% 6'(

.

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%

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(SAS 6.2, 1997)

*?:

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2C

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(Excel, 2003)

*?:

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ﺎﺘﻧ

ﺞﻳ

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AG)& H!

$:H % 0 M4 K(H 0%:

%FG!

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%

F. solani f. sp. phaseoli

K

AV: 8 "R%(

) ) g ( 0 @

H M36

ge :(

%

%FP(

0%!

) "=

g .(

+!

% S) v

Schaad et al.

(2001)

@%FP( $:H

Bacillus subtilis

* kV!

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O 0 %Π7

j $:H %:

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8+7 '/

+/6

F. solani f. sp. phaseoli

) (

M36 M38 /

M25 B2 K2 J2

M21 /

N2 /

M36 <@6 $ 7 4?! >&' '/ 8+7 /-

+/6

F. solani f. sp. phaseoli

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07

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Characteristic

$%&

(Reaction)

M25 M21

M38 M36

:;

<!2 <

=&5 Cell diameter>1µm

<

<

<

<

?-0 Motility

+ +

+ +

@;!

!72 Spore position

A A

A A

!0 2 : Acid from:

A!

D-Glucose +

+ +

+

<

!%

L-Arabinose +

+ +

+

!' D-Xylose

+ +

+ +

<!

D-Manitol +

+ +

+

A&

C0DE Gelatin hydrolase

+ +

+ +

A&

26 Starch hydrolase

+ +

+ +

A&

CF Casein hydrolase

+ +

+ +

!0

<&

Indole formation -

- -

-

D0 Catalase

+ +

+ +

G 9 Nitrat reduction

+ +

+ +

* H!I2 Growth at 5oC

- -

- -

* H!I2 Growth at 45oC

+ +

+ +

:PH Growth at pH

J / 5.7

+ +

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24. Sallam, N., Abd Elrazik, A. A., Hassan, M. & Koch, E. (2009). Powder formulations Bacillus subtilis, Trichoderma spp. and Conithyrium minitans for biocontrol of Onion White Rot. Phytopathology and Plant Protection, 42 (2), 142-147.

25. Saravanan, T., Bhaskaran, R. & Muthusamy, M. (2004). Pseudomonas fluorescens induced enzymological changes in banana roots (Cv. Rasthali) against Fusarium wilt disease. Journal of Plant Pathology, 3(2), 72-80.

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27. Schisler, D. A., Slininger, D. J., Behle, R. W. & Jakson, M. A. (2004). Formulation of Bacillus spp. for

(13)

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: 23 4 5/6 .5

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$ 7 8/- 8 ...

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biological control of plant disease. Phytopathology, 94, 1267-1271.

28. Shahrokhi, A. (2008). Study on the possible use of bacillus bacteria producing fnjicin antibiotic for the control of Rhizoctonia solani the casual agent of sugarbeet root rot. Ph. D. dissertation. University of Tehran, Iran.

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Communications in Agricultural Applied Biological Science, 71(2), 131-140.

30. Shrifi-Tehrani, A., Ahmad Zadeh, M., Farzaneh, V & Sarani, S-A. (2007). Evaluating the effect of powdery formulation of two isolates of Pseudomonas fluorescens in controlling the Rhizoctonia solani the casual agent of Brassica spp damping-off in field and greenhouse. Iranian Journal of Agriculture Science, 37 (6), 1121-1130. (In Farsi).

31. Spigel, Y. & Hall, R. (1982). Effects of pathogen species, inoculum concentration, temperature and soil moisture on bean root rot and plant growth. Canadian Journal. Plant pathology, 4, 1-7.

32. Weller, D. M. & Cook, R. J. (1983). Suppression of take-all of wheat by seed treatment with Pseudomonas fluorescens. Phytopathology, 73, 463-469.

Referensi

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