ﺳرﺮﺑ ﯽ ﺮﺛا ﻣ ﺪﺿ ﯿ ﺑوﺮﮑ ﯽ ﺳﻮﻧﺎﻧ ﯿ ﺎﭘ ﺮﺑ لﺎ ﯾ
ﺮﺳ هرﺎﺼﻋ ﻪ ﯾ
و ﺶ ﻮﻧﺎﻧ هرذ
ﺴﮐا ﯿ ور ﺪ ي
ﻞﮐﻮﺗ نﺎﺟﺮﻣ ﻠﻋ ،
1ﯽ ﺪﻤﺤﻣ ي
ﻧﺎﺛ
2
ﯽ
، ا
ﯾ ﺶﺨﺒﺣﺮﻓ نﺎﻤ
1
1 - ﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ﯽ
ﺎﻨﺻ و مﻮﻠﻋ هوﺮﮔ ،نﺎﭼﻮﻗ ﺪﺣاو ، ﯾ
اﺬﻏ ﻊ ﯾ ﯽ ا ،نﺎﭼﻮﻗ ، ﯾ ناﺮ
2 - ﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،نﺎﭼﻮﻗ ﺪﺣاو ،نﺎﮕﺒﺨﻧ و ناﻮﺟ ناﺮﮕﺸﻫوﮋﭘ هﺎﮕﺷﺎﺑ ﯽ
ا ،نﺎﭼﻮﻗ ، ﯾ ناﺮ
) رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 26 / 03 / 95 رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 20 / 04 / 96 (
ﮑﭼ ﯿ هﺪ
زرا فﺪﻫ ﺎﺑ ﺮﺿﺎﺣ ﺶﻫوﮋﭘ ﯾﺑﺎ
ﯽ ﻣﺪﺿ ﺮﺛا ﯿ ﺑوﺮﮑ ﯽ ﺳﻮﻧﺎﻧ ﯿ ﺎﭘ ﺮﺑ لﺎ ﯾ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪ ﯽ
ﺮﺳ ﯾ ﺶ رد اﺮﺷ ﯾ ﻂ ﺎﻣزآ ﯾ ﻫﺎﮕﺸ ﯽ ﺮﺘﮐﺎﺑ ﻪﺳﺮﺑاﺮﺑ رد ﻓﺎﺘﺳاي
ﯿ ،سﻮﺋروا سﻮﮐﻮﮐﻮﻠ
ﺷﺮﺷا ﯿ ﻠﮐ ﺎ ﯽ ﺮﺘﻧآ ﻼﻧﻮﻤﻟﺎﺳ ، ﯾ
ﺎﮑ ﺪﺷ مﺎﺠﻧا . هرﺎﺼﻋ ﮔﯿ هﺎ ﺮﺳ ﯾ ﺧ شور ﻪﺑ ﺶ ﯿ
اﺮﺑ و ﺪﻣآ ﺖﺳد ﻪﺑ نﺪﻧﺎﺴ ي
ﻌﺗ ﯿﯿ ﺮﺘﮐﺎﺑﺪﺿ ﺮﺛا ﻦ ﯾﺎﯾ
ﯽ نآ رﺎﺸﺘﻧا شور زا دﯾ
ﮏﺴ ﻪﺳرد
ﺖﻈﻠﻏ mg/ml 200 ، 250 و 300 اﺮﺑ ي هرﺎﺼﻋ ﻟﻮﻧﺎﺗا ﯽ ﮔﯿ هﺎ ﺖﻈﻠﻏ و 1000ppm
اﺮﺑ ي هرذﻮﻧﺎﻧ ﺴﮐا ﯿ ﺪ ور ي ﺪﺷ هدﺎﻔﺘﺳا .
ﺎﺘﻧ ﯾ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﮐ داد نﺎﺸﻧ ﺞ ﯽ
ﮔ ﯿ هﺎ
ﺮﺳ ﯾ اراد هرذﻮﻧﺎﻧ نوﺪﺑ ﺶ ي
مﺪﻋ ﻪﻟﺎﻫ ﺪﺷر هرذ ﻮﻧﺎﻧ ندوﺰﻓا ﺎﺑ ﺎﻣا دﻮﺒﻧ ﺴﮐا
ﯿ ور ﺪ ي ﻪﺑ هرﺎﺼﻋ ﮔﯿ هﺎ دﺮﮔ هﺪﻫﺎﺸﻣ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﯾ
ﺮﺘﮔرﺰﺑ ﻪﮐ ﺪ ﯾ
نآ ﻦ 23 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ
دﻮﺑ . ﮔﺪﻧرادزﺎﺑ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
) (MIC ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ و ﯽ
) (MBC ﻗر شور زا هدﺎﻔﺘﺳا ﺎﺑ ﯿ
زﺎﺳ ﻖ ي ﻣ ﯿ ﻌﺗ ثاﺮﺑوﺮﮑ ﯿﯿ
دﺮﮔ ﻦ ﯾ ﺪ . ا رد ﯾ هرﺎﺼﻋ نﻮﻣزآ ﻦ
ﮔﯿ ﺎﭘ ﺮﺑ هﺎ ﯾ هرذﻮﻧﺎﻧ ﻪ ﺴﮐا ﯿ ور ﺪ ي ﺮﺗﻻﺎﺑ ﯾ ﺑ تاﺮﺛا ﻦ ﮔﺪﻧرادزﺎ ﯽ ) 5/
12 ﻣ ﯿﻠ ﯽ ﻣﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ( ﮔﺪﻨﺸﮐ و ﯽ ) 25 ﻣ ﯿﻠ ﯽ مﺮﮔ ﻣﺮﺑ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ( ﺮﺘﮐﺎﺑ ﺮﺑاﺮﺑ رد ي
ﻓﺎﺘﺳا ﯿ سﻮﮐﻮﮐﻮﻠ
سﻮﺋروا داد نﺎﺸﻧ دﻮﺧ زا .
ﺎﺘﻧ زا ﯾ ﻨﭼ ﺞ ﯿ ﻣﺮﺑ ﻦ ﯽ آ ﯾ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﻪﮐ ﺪ ﯽ
ﺪﻨﭼﺮﻫ اراد ي ﺻﺎﺧ ﯿ ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖ ﯽ
ﮔﺪﻨﺸﮐ و ﯽ ﻟ دﻮﺒﻧ ﯿ هرذﻮﻧﺎﻧ ندوﺰﻓا ﺎﺑ ﻦﮑ ﺴﮐا
ﯿ ﺪ ور ي
ﮔرﺎﮑﺑ نﺎﮑﻣا ﯿﺮ
ي ﺒﻃ هﺪﻧراﺪﻬﮕﻧ ناﻮﻨﻋ ﻪﺑ و ﺎﻫ نژﻮﺗﺎﭘ لﺮﺘﻨﮐ ﺖﻬﺟ نآ ﯿﻌ
ﯽ ﺎﻨﺻرد ﯾ زﺎﺳوراد ﻊ ي اﺬﻏ و ﯾ ﯽ ﺖﺳا هدﺎﻔﺘﺳا ﻞﺑﺎﻗ .
ﻠﮐ ﯿ نﺎﮔژاوﺪ ﻣﺪﺿ صاﻮﺧ : ﯿ
ﺑوﺮﮑ ﯽ ﺮﺳ هرﺎﺼﻋ ، ﯾ
ﺴﮐا ،هرذﻮﻧﺎﻧ ،ﺶ ﯿ
ور ﺪ ي
لﻮﺌﺴﻣ
تﺎﺒﺗﺎﮑﻣ :
1 - ﻪﻣﺪﻘﻣ
رﺎﺗ لﻮﻃ رد ﯾ
ﺮﺸﺑ ﺦ ي ﺴﺑ ﯿ رﺎ ي ﺑ زا ﯿ رﺎﻤ ي ﺎﻫ ي ﻧﻮﻔﻋ ﯽ ﺘﻨﺳرﻮﻃ ﻪﺑ ﯽ
ﺎﻫوراد ﺎﺑ ي ﮔ ﯿ ﻫﺎ ﯽ هﺪﺷ نﺎﻣرد رﻮﻃ ﻪﺑ ﺪﻧا
ي ﺴﺑ رد هزوﺮﻣا ﻪﮐ ﯿ
رﺎ ي
ﺎﻫرﻮﺸﮐ زا ي
ﺎﻫوراد ﻪﻌﺳﻮﺗ لﺎﺣ رد ي
ﮔ ﯿ ﻫﺎ ﯽ ﻠﺻا ﺶﻘﻧ ﯽ
ار
ﻟوا نﺎﻣرد ﺖﻬﺟ ﯿ
ا ﻪ ﯾ ﻣ ﺎﻔ ﯽ ﺪﻨﻨﮐ ] 1 [ . ﺑﯿ رﺎﻤ ي ﺎﻫ ي ﺷﺎﻧ ﯽ فﺮﺼﻣ زا
اﺬﻏ داﻮﻣ ﯾ ﯽ ﻣﻮﻤﻋ ﺖﻣﻼﺳ ﺎﺑ ﻂﺒﺗﺮﻣ ﻢﻬﻣ دراﻮﻣ زا هدﻮﻟآ ﯽ
ﻣ ﯽ ﺪﺷﺎﺑ
ز ﺶﻫﺎﮐ ﺖﻬﺟ و ﯾ
نﺎ ﺎﻫ ي دﺎﺼﺘﻗا ي و ﻧﺎﺟ تاﺮﻄﺧ ﯽ
ﺷﺎﻧ ﯽ ا زا ﯾ ﻦ
ﺑﯿ رﺎﻤ ي ﺒﻃ داﻮﻣزا هدﺎﻔﺘﺳا ،ﺎﻫ ﯿﻌ
ﯽ ﮐﺮﺗ ناﻮﻨﻋ ﻪﺑ ﯿ
ﺪﺿ تﺎﺒ ﻣﯿ ،بوﺮﮑ
ﺷور ﯽ ﻣ ﺮﻈﻧ ﻪﺑ ﺮﺛﺆﻣ ﯽ
ا ﺮﺑ هوﻼﻋ ﻪﮐ ﺪﺳر ﯾ
ﻣ ،ﻦ ﯽ ﻪﺑ ﺮﺠﻨﻣ ﺪﻧاﻮﺗ
اﺰﻓا ﯾ رﺎﮔﺪﻧﺎﻣ ﺶ ي
ددﺮﮔ لﻮﺼﺤﻣ .
ا رد ﯾ ﻣ ﻦ ﯿ هرﺎﺼﻋ نﺎ ﺎﻫ ي ﮔ ﯿ ﻫﺎ ﯽ
اراد ي ﻣﺪﺿ صاﻮﺧ ﯿ
ﺑوﺮﮑ ﯽ فﺮﺼﻣ ﻪﮐ ﺎﺠﻧآ زا و هدﻮﺑ ﻪﺑ نﺎﮔﺪﻨﻨﮐ
اﯾ ﻤﻨ ﯽ اﺬﻏ داﻮﻣ ﯾ ﯽ وﺎﺣ ي ﺰﺘﻨﺳ هﺪﻧراﺪﻬﮕﻧ ي
ﻤﻃا ﯿ ﻓﺎﮐ نﺎﻨ ﯽ ،ﺪﻧراﺪﻧ
ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ لﺎﺒﻘﺘﺳا درﻮﻣ ﺎﻫ نآ دﺮﺑرﺎﮐ اﺬﻟ ]
2 [ . و ﺎﻫ هرﺎﺼﻋ
ﺲﻧﺎﺳا ﺎﻫ ي ﮔﯿ ﻫﺎ ﯽ ﻟد ﻪﺑ ﯿ ﺎﻫ هوﺮﮔ دﻮﺟو ﻞ ي
ﻟﻮﻨﻓ لﺎﻌﻓ ﯿ رد ﮏ
ﮐﺮﺗ ﯿ ﺎﺴﮐا ﺪﺿ صاﻮﺧ ،دﻮﺧ ﺐ ﯾ
ﺸ ﯽ ﻣ ﺪﺿ و ﯿ ﺑوﺮﮑ ﯽ نﺎﺸﻧ دﻮﺧ زا
ﻣ ﯽ ﺪﻨﻫد ] 3[
.
ﺮﺳ ﯾ مﺎﻧ ﺎﺑ ﺶ ﻤﻠﻋ ﯽ Eremurus spectabiils ﮔ
ﯿ ﻫﺎ ﯽ بﺎﻌﻟ وراد
ﺘﺣارﺎﻧ نﺎﻣرد رد ﺮﺛﻮﻣ ﯽ
ﺎﻫ ي ﺪﺒﮐ ﻣز رد ﺖﺳا هﺪﻌﻣ و ﯿ
صاﻮﺧ ﻪﻨ
ﻣﺪﺿ ﯿ ﺑوﺮﮑ ﯽ ﺮﺳ ﯾ ﻘﺤﺗ ﺶ ﯿ ز تﺎﻘ ﯾ دﺎ ي ﺖﺳا هﺪﺸﻧ مﺎﺠﻧا .
ﻧﺎﻌﻨﮐ ﯽ و
نارﺎﮑﻤﻫ ) 2015 ( ﺘﻧآ صاﻮﺧ ﯽ ﺮﺘﮐﺎﺑ ﯾ ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ لﺎ ﯽ
ﮔﯿ ﺮﺳ هﺎ ﯾ ﺶ
ور ار ي ﺎﻫ نژﻮﺗﺎﭘ ي
ﺳرﺮﺑ ﻒﻠﺘﺨﻣ ﯽ
ﺪﻧدﻮﻤﻧ ] 4 [.
را و ﻪﻌﺳﻮﺗ ﺪﺿ صاﻮﺧ ﺎﺑ داﻮﻣ ءﺎﻘﺗ
ﻣﯿ ﺑوﺮﮑ ﯽ دزا ﯾ فاﺪﻫا وﺰﺟ زﺎﺑﺮ
ﮑﺷﺰﭘ مﻮﻠﻋ رد ﯽ
ﺖﺳا هدﻮﺑ اﺬﻏ ﺖﻌﻨﺻ و .
ﻣﺪﺿ ﻞﻣاﻮﻋ ﯿ
ﺑوﺮﮑ ﯽ
ﮐﺮﺗ سﺎﺳاﺮﺑ ﯿ
ﺷ ﺐ ﯿﻤ ﯿﺎﯾ ﯽ ﻣﺪﺿ ﻞﻣاﻮﻋ ﻪﺘﺳد ود ﻪﺑ ﯿ
ﺑوﺮﮑ ﯽ ﻟآ ﯽ
ﻏو ﯿ ﻟآﺮ ﯽ ﺴﻘﺗ ﯿ ﻣ ﻢ ﯽ ﺴﺑ ﺪﻧﻮﺷ ﯿ رﺎ ي ﻣﺪﺿ ﻞﻣاﻮﻋ ﺺﻗاﻮﻧ زا ﯿ
ﺑوﺮﮑ ﯽ
ﻟآ ﯽ دوﺪﺤﻣ ﻪﺑ ﺮﺠﻨﻣ ﯾ
ﻣ ﻪﮐ ﺖﺳا هﺪﺷ ﺎﻬﻧآ هدﺎﻔﺘﺳا ﺖ ﯽ
ﻪﺑ ناﻮﺗ
ﺎﭘ ﺖﻣوﺎﻘﻣ ﯾﯿ
ﻠﺑﺎﻗ ،تراﺮﺣ ﻞﺑﺎﻘﻣ رد ﺎﻬﻧآ ﻦ ﯿ
ﺰﺠﺗ ﺖ ﯾ ﺘﻧ رد و ﻪ ﯿ ﻪﺠ
ﻧﯿ ﻪﻤ ﺎﭘ ﺮﻤﻋ ﯾﯿ دﻮﻤﻧ هرﺎﺷا ﻻﺎﺑ ًﺎﺘﺒﺴﻧ رﺎﺸﻓو تراﺮﺣرد ﻦ ]
5 [.
ﺑ زا ﯿ ﻦ
ﻣﺪﺿ ﻞﻣاﻮﻋ ﯿ
ﺑوﺮﮑ ﯽ ﻏ ﯿ ﻟآﺮ ﯽ ﺴﮐا ﯿ ﺴﺑ تارذ ﻮﻧﺎﻧ ﺪ ﯿ
ﻪﺟﻮﺗ درﻮﻣ رﺎ
ﻪﺘﻓﺮﮔ راﺮﻗ هدﺎﻔﺘﺳا درﻮﻣ ﻒﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣردو هﻮﺑ ﺪﻧا
] 6 [.
تارذ
ﻏﯿ ﻟآﺮ ﯽ ﻧا ﺎﺑ ﻟد ﻪﺑ ﻮﻧﺎﻧ هزاﺪ ﯿ
ﻞ ﺒﻃ ﯿ ﮐﺮﺗو ﺖﻌ ﯿ صاﻮﺧ ،هدﺎﺳ ﺐ
ﻓﯿ ﺰﯾ ﮑ ﯽ ﺷو ﯿﻤ ﯿﺎﯾ ﯽ ﯾ ﻧﺎﺴﮑ ﯽ ﻣ زوﺮﺑ ﯽ ﺪﻨﻫد ] 7 [.
ﯾ ﮑ ﯽ ﻢﻬﻣ زا ﺮﺗ ﯾ ﺴﮐا تارذﻮﻧﺎﻧ ﻦ ﯿ
ﺪ ور ي ﺴﺑ رد ﻪﮐ ﺖﺳا ﯿ
رﺎ ي زا
ﻘﻣ رد ﺎﻫرﻮﺸﮐ ﯿ
ﺘﻌﻨﺻ سﺎ ﯽ ﻟﻮﺗ ﯿ ﻣ هدﺎﻔﺘﺳا و ﺪ ﯽ
دﻮﺷ . ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ اﯾ
و ﻦ ﯾ ﮔﮋ ﯽ هرذﻮﻧﺎﻧ ﺴﮐا ﯿ ور ﺪ ي ناﻮﻨﻋ ﻪﺑ ﯾ
ﮑ ﯽ ﺮﺗدﺮﺑرﺎﮐﺮﭘ زا ﯾ
ﻦ
ﻧﺎﻧ اﺮﺑ تارذﻮ ي
ﺮﺘﮐﺎﺑ ﺎﺑ ﻪﻠﺑﺎﻘﻣ ي
ﺎﻫ ي مﺮﮔ ﻔﻨﻣ ﯽ درﻮﻣ ﺖﺒﺜﻣ و
ﺖﺳا ﻪﺘﻓﺮﮔ راﺮﻗ هدﺎﻔﺘﺳا .
ﻧﺎﮑﻣ ﯿ ﻠﺻا ﻢﺴ ﯽ ﺛﺄﺗ ﯿ ورﺮﺑ تارذﻮﻧﺎﻧ ﺮ ي
ﺮﺘﮐﺎﺑ ي ﺮﻃ زا ﺎﻫ ﯾ ﺳآ ﻖ ﯿ ﻪﺑ ﺐ ﺌﺗوﺮﭘ ،DNA
ﯿ ﺮﺨﺗ و ﻦ ﯾ د ﺐ ﯾ هراﻮ
ﻟﻮﻠﺳ ﯽ ﻣ ﯽ ﺪﺷﺎﺑ . ﺗاﺰﻠﻓ ﯽ ﻈﻧ ﯿ ور ،ﺲﻣﺮ ي ﻞﻣاﻮﻋ ﻪﻠﻤﺟ زا ﺖﻟﺎﺒﮐ ،
ﻣ ﺪﺿ داﻮﻣ ﯿ
ﺑوﺮﮑ ﯽ اﺮﺑ ﺮﺛﺆﻣ ي ﻤﮑﺗ ﯿ ﺎﺧ ﻞ ﺻ ﯿ ﺮﺘﮐﺎﺑ ﺪﺿ ﺖ ﯾﺎﯾ
ﯽ
ﻣ رﺎﻤﺷ ﻪﺑ تﺎﺟﻮﺴﻨﻣ ﯽ
ﺪﻧور ] 7[
.
ﻪﻌﻟﺎﻄﻣرد ﻧﺎﺧ ﯽ تارذﻮﻧﺎﻧ زا هدﺎﻔﺘﺳا ﺎﺑ ﻪﮐ ﺪﺷ هداد نﺎﺸﻧ نارﺎﮑﻤﻫو
ﻣ ﯽ ﺮﺘﮐﺎﺑ ﺪﺷر ناﻮﺗ ي
ﺎﻫ ي ﺑ ﯿ رﺎﻤ ي از ﯾ ﯽ نﻮﭼ ﺷﯿ
،ﻼﮕ ﺮﺷا ﯾ ﺸ ﯿ ﺎ
ﻓﺎﺘﺳاو ﯿ سﻮﮐﻮﮐﻮﻠ دﺮﮐرﺎﻬﻣار
] 1 [ . ﺎﺳ ﯾ ﺰﺘﻨﺳ تارذﻮﻧﺎﻧ ﮏﭼﻮﮐ ﺰ
اﺰﺳ ﻪﺑ ﺶﻘﻧ هﺪﺷ ﯾ
ﯽ ﻣ رد ﯿ ﻣﺪﺿ ﺮﺛا ناﺰ ﯿ
ﺑوﺮﮑ ﯽ ا ﯾ ا تارذﻮﻧﺎﻧ ﻦ ﯾ
ﺎﻔ
ﻣ ﯽ ﺪﻨﮐ ] 8 [.
ﻣز رد ﯿ ﻣﺪﺿ ﺮﺛا ﻪﻨ ﯿ
ﺑوﺮﮑ ﯽ ﻧﻮﮔﺎﻧﻮﮔ تﺎﻌﻟﺎﻄﻣ تارذﻮﻧﺎﻧ ﯽ
ﺖﺳا ﻪﺘﻓﺮﮔ ترﻮﺻ .
ﻨﭽﻤﻫ ﯿ ﻪﮐ ﻪﻧﻮﮕﻧﺎﻤﻫ ﻦ رد ﺪﺷ هرﺎﺷا
ﻣﺪﺿ ﺮﺛا صﻮﺼﺧ ﯿ
ﺑوﺮﮑ ﯽ ﮔ ﯿ ﺮﺳ هﺎ ﯾ ﺗﺎﻌﻟﺎﻄﻣ نآ هرﺎﺼﻋ و ﺶ ﯽ
ﻟ ﺖﺳا ﻪﺘﻓﺮﮔ ترﻮﺻ ﯿ
ا نﺎﻣﺰﻤﻫ ﺮﺛا ﻦﮑ ﯾ
ﻣﺪﺿ ﻞﻣﺎﻋ ود ﻦ ﯿ
بوﺮﮑ
ﺳرﺮﺑ ﯽ زا فﺪﻫ اﺬﻟ و ﺖﺳا هﺪﺸﻧ ا مﺎﺠﻧا
ﯾ ﺳرﺮﺑ ﺶﻫوﮋﭘ ﻦ ﯽ
ﺛﺎﺗ ﯿ ﺮ
ا ﻞﺑﺎﻘﺘﻣ ﯾ ور ﺮﺑ ﻞﻣﺎﻋ ود ﻦ ي
ﺧﺮﺑ ﯽ ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻤﻫا ﺰﺋﺎﺣ ﯿ رد ﺖ
دﻮﺑ اﺬﻏ ﺖﻌﻨﺻ .
2 - شورو داﻮﻣ ﺎﻫ
2 - 1 - روآ ﻊﻤﺟ ي
ﮔ ﯿ ندﺮﮐ ﮏﺸﺧو هﺎ
ﮔﯿ ﺮﺳ هﺎ ﯾ درا رد ﺶ ﯾ
ﺖﺸﻬﺒ 1394 درﻮﻨﺠﺑﺮﻬﺷرﺎﻬﺑ هﻮﮐ ﻪﻘﻄﻨﻣزا
ﻊﻤﺟ روآ ي ور ﺮﺑ و ي ﻤﺗ ﻪﭼرﺎﭘ ﯿ ر ﮏﺸﺧو ﺰ ﯾ
ﺎﺳ رد و ﻪﺘﺨ ﯾ
ﻪ
ﺒﺳﺎﻨﻣ ﯽ دﺮﮔ ﻦﻬﭘ ﯾ ﺪ ) ﺪﺷ دﺎﺑ ﺪﻗﺎﻓ ﯾ
ﺖﺑﻮﻃر و ﺪ .(
ﮔﯿ هﺪﺷ ﮏﺸﺧ هﺎ
ﺳآ هﺎﮕﺘﺳد ﻂﺳﻮﺗ ار ﯿ
ﻗﺮﺑ بﺎ ﯽ فﺮﻇ رد و هدﻮﻤﻧ ردﻮﭘ ﺗ ﯿ
رد هﺮ
هرﺎﺼﻋ نﺎﻣز ﺎﺗ ﻪﺘﺴﺑ ﮔﯿ
ﺮ ي راﺪﻬﮕﻧ ي ﺪﺷ .
2 - 2 - هرﺎﺼﻋ ﮔﯿ ﺮ ي
هرﺎﺼﻋ ﮔﯿ ﺮ ي ﺧ شور ﻪﺑ ﯿ
ا ﻪﺑ ،ﺖﻓﺮﮔ ترﻮﺻ نﺪﻧﺎﺴ ﯾ
رﻮﻈﻨﻣ ﻦ 50
ﮔ مﺮﮔ ﯿ رد ار هﺪﺷ دﺮﺧ ﮏﺸﺧ هﺎ ﯾ
ﻦﻟﺎﺑ ﮏ 1000 ﻣ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ي
رﯾ ور و ﻪﺘﺨ ي نآ 500 ﻣ ﯿﻠ ﯽ ﻟ ﯿ تﺪﻣ ﻪﺑ لﻮﻧﺎﺗا لﻼﺣ ﺮﺘ 72
ﺖﻋﺎﺳ
ﺪﺷ هدﺰﻤﻫ ﺖﻋﺎﺳ ود ﺮﻫ و ﻪﻓﺎﺿا .
ﮔ و لﻼﺣ طﻮﻠﺨﻣ ﺲﭙﺳ ﯿ
هﺎ
ﻓﺎﺻ ﻂﺳﻮﺗ ﯽ
ﺬﻏﺎﮐ ي زﺎﺳاﺪﺟ ي رﺎﺗور ﻂﺳﻮﺗ لﻼﺣو ي
ﻼﺧ ﺖﺤﺗ
) V-855Buchi (
فﺬﺣ دﺮﮔ ﯾ ﺎﻣد رد ﺲﭙﺳ و ﺪ ي
38 ﻪﺟرد
ﺘﻧﺎﺳ ﯿ ءﻼﺧ ﺖﺤﺗ نوآ رد داﺮﮕ )
Memmert-Germany (
ًﻼﻣﺎﮐ
نﻮﻣزآ ﺖﻬﺟ و ﮏﺸﺧ ﺎﻫ
ي ﺪﻌﺑ ي ﺷ فﺮﻇ رد ﯿ
ﻪﺸ ا ي ﺗ ﯿ هﺮ
راﺪﻬﮕﻧ ي دﺮﮔ ﯾ ﺪ ] 9 [.
2 - 3 - زﺎﺳ هدﺎﻣآ ي
ﺮﺘﮐﺎﺑ ﺖﺸﮐ ﯾﺎ
ﯾ ﯽ
ﻮﺳ ﯾﻪ ﺎﻫ ي ﻣ ﯿ ﺑوﺮﮑ ﯽ درﻮﻣ هدﺎﻔﺘﺳا ﻞﻣﺎﺷ ﻓﺎﺘﺳا ﯿ سﻮﮐﻮﮐﻮﻠ
سﻮﺋروا ) (PTCC1431 ﻼﻧﻮﻤﻟﺎﺳ ،
ﺮﺘﻧآ ﯾ ) ﺎﮑ (PTCC1709 و
ﺷﺮﺷا ﯿ ﻠﮐﺎ )ﯽ PTCC1399 (
دﻮﺑ . ﺖﻬﺟ ﻬﺗ ﯿﻪ ﺖﺸﮐ 24
،ﻪﺘﻋﺎﺳ
ﻂﺳﻮﺗ پﻮﻟ ﺮﺘﺳا ﯾ ﻞ زا ﺖﺸﮐ ردﺎﻣ راﺪﻘﻣ ي ﻪﺘﺷادﺮﺑ و ور ي ﺤﻣ ﯿ ﻂ
ﺖﺸﮐ ﺮﻟﻮﻣ ﻫ ﯿ نﻮﺘﻨ رﺎﮔآ ) كﺮﻣ نﺎﻤﻟآ ( ﻪﺑ ﺖﻟﺎﺣ ﻄﺧ ﯽ ﺖﺸﮐ هداد
ﺪﺷ و ﺲﭙﺳ ﻪﺑ ﺖﻟﺎﺣ ﻪﻧوراو رد رﻮﺗﺎﺑﻮﮑﻧا 37 ﻪﺟرد ﺘﻧﺎﺳ ﯽ داﺮﮔ ﻪﺑ
تﺪﻣ 24 ﺖﻋﺎﺳ راﺮﻗ و درﻮﻣ هدﺎﻔﺘﺳا رد نﻮﻣزآ ﺎﻫ ي ﻣ ﯿ ﺑوﺮﮑ ﯽ
دﺮﮔ ﯾ ﺪ . از ﻮﺳ ﯾﻪ ﺎﻫ ي قﻮﻓ ﺴﻧﺎﭙﺳﻮﺳ ﯿ نﻮ ﻣ ﯿ ﺑوﺮﮑ ﯽ ﺎﺑ شرﺎﻤﺷ
108
× 5/
1 ﻪﺑ شور تروﺪﮐ ﺠﻨﺳ ﯽ و ﻂﺳﻮﺗ دراﺪﻧﺎﺘﺳا ﻧﯿ ﻢ ﮏﻣ
ﺪﻨﻟرﺎﻓ ﻬﺗ ﯿﻪ ﺪﺷ . ] 10 [.
ﺴﻧﺎﭙﺳﻮﺳ ﯿ ﻧ دراﺪﻧﺎﺘﺳا نﻮ ﯿ
ز لﻮﻣﺮﻓ ﺎﺑ ﺪﻨﻟرﺎﻓ ﮏﻣ ﻢ ﯾﺮ
: 5/
0 ml BaCl2 /0048 M (1/ 175 w/v BaCl2. H2O) +99/ 5 ml H2SO4 0/18
2 - 4 - زﺎﺳ هدﺎﻣآ ي
هرذﻮﻧﺎﻧ ﺎﺑ هرﺎﺼﻋ لﻮﻠﺤﻣ
ﺖﻈﻠﻏ ﺎﺑ ردﺎﻣ لﻮﻠﺤﻣ ﻪﺑ mg/ml
400 ﻟﻮﻧﺎﺗا هرﺎﺼﻋ زا ﯽ
، ppm
1000 ﺴﮐا هرذ ﻮﻧﺎﻧ ﯿ
ورﺪ ي ﺪﺷ ﻪﻓﺎﺿا .
ا ﻪﺑ ﯾ ﺗﺮﺗ ﻦ ﯿ ﺖﻈﻠﻏﺮﻫ ﺐ
ﻬﺗدﻮﺧﺮﺗﻻﺎﺑ ﺖﻈﻠﻏزا ﯿ
ﻣ ﻪ ﯽ دﻮﺷ . ﺎﻫ ﺖﻗر ي هرﺎﺼﻋ ﻒﻠﺘﺨﻣ
ﻞﻣﺎﺷ mg/ml 400 - 200 - 100 - 50 - 25 - 5/
12 - 25 /6 - 125 / 3 -
5625 /1 - 78125 /0 زﺎﺳ هدﺎﻣآ ي دﺮﮔ ﯾ ﺪ ] 10 [.
2 - 5 - د رﺎﺸﺘﻧا شور ﯾ
ﮏﺴ
ﺎﭙﺳﻮﺳ اﺪﺘﺑا ﺮﺘﮐﺎﺑ نﻮﺴﻧ
ﯾﺎﯾ ﯽ ﻧ لدﺎﻌﻣ تروﺪﮐ ﺎﺑ ﯿ
ﻬﺗ ﺪﻨﻟرﺎﻓ ﮏﻣ ﻢ ﯿ
،ﻪ
ﺮﺘﺳا پآﻮﺳ زا هدﺎﻔﺘﺳا ﺎﺑ ﺲﭙﺳ ﯾ
ور ﻞ ي ﻠﭘ ﺢﻄﺳ ﯿ ﺎﻫ ﺖ ي وﺎﺣ ي
ﺤﻣ ﯿ ﻫ ﺮﻟﻮﻣ ﺖﺸﮐ ﻂ ﯿ
رﺎﮔآ نﻮﺘﻨ ) نﺎﻤﻟآ كﺮﻣ (
ﺪﺷ مﺎﺠﻧا ﺖﺸﮐ ﻞﻤﻋ .
دﯾ ﺬﻏﺎﮐ ﮏﺴ ي
ﺮﻄﻗ ﺎﺑ 6 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ﺐﻃ ﻦﺗﺎﭘ ﺖﮐﺮﺷ ﺖﺧﺎﺳ ) (
وﺎﺣ ي 15 ﻣﯿ ﻟوﺮﮑ ﯿ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ ﺮﺘ ﯽ
ﺖﻈﻠﻏ رد ﺎﻫ
ي mg/ml 200 -
250 - 300 ﻨﭽﻤﻫ ﯿ ﺎﻫ ﻪﻧﻮﻤﻧ ﻦ ي
وﺎﺣ ي 1000ppm هرذﻮﻧﺎﻧ
ﻪﺑ و ﻪﺘﺸﻏآ ور
ي ﻠﭘ ﯿ تﺪﻣ ﻪﺑ و ﻞﻘﺘﻨﻣ ﺖ 24
رﻮﺗﺎﺑﻮﮑﻧا رد ﺖﻋﺎﺳ ﺘﻧﺎﺳ ﻪﺟرد 37
ﯽ ﻪﻧﺎﺨﻣﺮﮔ داﺮﮔ راﺬﮔ
ي دﺮﮔ ﯾ ﺪ . د زا ﯾ ﺘﻧآ ﮏﺴ ﯽ
ﺑﯿ ﺗﻮ ﯿ ﻨﭘ ﮏ ﯽ ﺳ ﯿﻠﯿ د زاو ﺖﺒﺜﻣ لﺮﺘﻨﮐ ناﻮﻨﻋ ﻪﺑ ﻦ ي
ﺘﻣ ﯿ ﺎﺴﮐﻮﻔﻟﻮﺳ ﻞ ﯾ
ﺪ
ﻔﻨﻣ لﺮﺘﻨﮐ ناﻮﻨﻋ ﻪﺑ ﯽ
ﺪﺷ هدﺎﻔﺘﺳا .
راﺬﮔ ﻪﻧﺎﺨﻣﺮﮔ زا ﺲﭘ ي
ﺮﻄﻗ ،
هزاﺪﻧا درﻮﻣ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﮔﯿ
ﺮ ي ﺖﻓﺮﮔراﺮﻗ ]
11 [ .
2 - 5 - ﻌﺗ ﯿﯿ ﮔﺪﻧرادزﺎﺑ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦ
1
ﯽ ) (MIC
ﻣ شور ﻪﺑ ﯿ
ادوﺮﮑ ﯾ ثاﺮﺑ ﻦﺷﻮﻠ
راﺪﻘﻣ 200L
ﺎﻫ ﺖﻈﻠﻏ زا ي
ﺳﻮﻧﺎﻧ ﻒﻠﺘﺨﻣ ﯿ
ﻟﻮﻧﺎﺗا هرﺎﺼﻋ لﺎ ﯽ
) ﻬﺗ ﯿ ﺤﻣ رد هﺪﺷ ﻪ ﯿ
ﻫ ﺮﻟﻮﻣ ﺖﺸﮐ ﻂ ﯿ
ثاﺮﺑ نﻮﺘﻨ (
ﻬﺗ ﺖﻬﺟ اﺪﺘﺑا ﯿ
ﻪ
ردﺎﻣ لﻮﻠﺤﻣ )
ﺖﻈﻠﻏ ﺎﺑ mg/ml 400 ( ﻟﻮﻧﺎﺗا هرﺎﺼﻋ زا ﯽ
ﻬﺗ ﯿ و ﻪ
ﺲﭙﺳ ﺖﻈﻠﻏ ﺎﻫ ي ﺮﺳ ﯾﻟﺎ ﯽ 100-200) -
50 - 25 - 5/
12 - 25 / 6 -
125 / 3 - 5625 /1 - 78125 /0 ﻣ ﯿﻠ ﯽ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟ ﯿ ﺮﺘ ( ﻬﺗ ﯿ ﻪﺑ و ﻪ
ﺎﻫ ﮏﻫﺎﭼ ي ﻣ ﯿ ﻠﭘوﺮﮑ ﯿ ﺖ 96 ا ﻪﻧﺎﺧ ي ﺪﺷ ﻞﻘﺘﻨﻣ .
رد ﺮﺧآ ﻪﺑ ﻪﻤﻫ
ﺎﻫ ﮏﻫﺎﭼ 20μl
ﺴﻧﺎﭙﺳﻮﺳ ﯿ نﻮ ﻣ ﯿ ﺑوﺮﮑ ﯽ لﻮﻠﺤﻣ لدﺎﻌﻣ تروﺪﮐ ﺎﺑ
ﻧﯿ ﻢ ﮏﻣ ﺪﻨﻟرﺎﻓ دﺮﮔ ﻪﻓﺎﺿا ﯾ
ﺪ ] 10 .[
،ﺖﺒﺜﻣ لﺮﺘﻨﮐ ناﻮﻨﻋ ﻪﺑ
داﺪﻌﺗرد ي ﻪﻧﺎﺧزا ﺎﻫ ي ﻠﭘ ﯿ ﺖ 200 ﺎﻣ ﯾ ﻟوﺮﮑ ﯿ ﺤﻣزا ﺮﺘ ﯿ ﺖﺸﮐ ﻂ
ﻫﺮﻟﻮﻣ ﯿ ،ثاﺮﺑ نﻮﺘﻨ 20
ﻣﯿ ﻟوﺮﮑ ﯿ ﺴﻧﺎﭙﺳﻮﺳﺮﺘ ﯿ
ﺮﺘﮐﺎﺑ نﻮ ي
و 50
ﻣﯿ ﻟوﺮﮑ ﯿ ﺘﻧآﺮﺘ ﯽ ﺑﯿ ﺗﻮ ﯿ ﻨﭘ ﮏ ﯽ ﺳ ﯿﻠﯿ ﻦ ﺪﺷ ﻪﻓﺎﺿا .
اﺮﺑ ي ﻤﻃا ﯿ ا زا نﺎﻨ ﯾ ﻪﮑﻨ
لﻼﺣ هدﺎﻔﺘﺳا درﻮﻣ (DMSO)
ﮔﺪﻨﺸﮐ ﺮﺛا ﯽ
ور ﺮﺑ ي
ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﻪﻧﺎﺧ زا ،ﺪﺷﺎﺑ ﻪﺘﺷاﺪﻧ ﺎﻫ ﺎﻫ
ي وﺎﺣ ي 200
ﺎﻣ ﯾ ﻟوﺮﮑ ﯿ ﺤﻣ زا ﺮﺘ ﯿ ﻫﺮﻟﻮﻣ ﺖﺸﮐ ﻂ ﯿ
ﺮﺘﮐﺎﺑ و لﻼﺣ و ثاﺮﺑ نﻮﺘﻨ ي
ﻪﺑ
ناﻮﻨﻋ ) ﻔﻨﻣ لﺮﺘﻨﮐ ﯽ ( دﺮﮔ هدﺎﻔﺘﺳا ﯾ
ﺪ .
ﻠﭘﯿ ﺖ 96 تﺪﻣ ﻪﺑ ﻪﻧﺎﺧ 24
ﻪﻧﺎﺨﻣﺮﮔ رد ﺖﻋﺎﺳ 37
ﺘﻧﺎﺳ ﻪﺟرد ﯿ
داﺮﮕ
ﺪﺷ هدادراﺮﻗ .
ا زا ﺲﭘ ﯾ ﻪﻧﺎﺧ مﺎﻤﺗ ﻪﺑ تﺪﻣ ﻦ ﺎﻫ
ي ﻠﭘ ﺮﻫ ﯿ ﺖ 50
ﺎﻣ ﯾ ﻟوﺮﮑ ﯿ ﺮﺗ فﺮﻌﻣ زا ﺮﺘ ي
ﻨﻓﯿ ﻞ ﻟوزاﺮﺘﺗ ﯿ مﻮ اﺮﻠﮐ ﯾ ﺪ فﺮﻌﻣ) ﮕﻧر ﯽ
ﻣ ﺪﺷر ﯿ بوﺮﮑ ( ﻪﻓﺎﺿا تﺪﻣ ﻪﺑ ًادﺪﺠﻣ و 3
ﻪﻧﺎﺨﻣﺮﮔرد ﺖﻋﺎﺳ
ﺪﺷ هدادراﺮﻗ .
ﺲﭘ رد ،ﻪﻧﺎﺨﻣﺮﮔ زا جوﺮﺧ زا درﺮﻫ
ﯾ ﻪﺑ طﻮﺑﺮﻣ ﻒ
ﺖﻈﻠﻏ ﺎﻫ ي ﻒﻠﺘﺨﻣ ﯾ ﻟوا ،هرﺎﺼﻋ ﮏ ﯿ
ﺘﻈﻠﻏ ﻦ ﯽ ﮓﻧر نآ رد ﻪﮐ
ﮑﺸﺗ ﺰﻣﺮﻗ ﯿ ناﻮﻨﻋ ﻪﺑ ،ﺪﺷﺎﺑ هﺪﺸﻧ ﻞ ﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد MIC
] 12 [.
Table 1 Inhibition zones (mm) of different bacteria affected by Eremurus spectabiils extract
Treatment Extract
200 mg/ml
extract 250 mg/ml
extract 300mg/ml
penicillin positive control
DMSO
negative control Staphylococcus
aureus 0 0 0 31 0
Salmonella enterica 0 0 0 20 0
Escherichia coli 0 0 0 10 0
2 - 6 - ﻌﺗ ﯿﯿ ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦ ﯽ
) ( MBC
2راﺪﻘﻣ µl 10 ﺮﻫ زا ﯾ ﻣ زا ﮏ ﯿ ﻠﭘوﺮﮑ ﯿ ﺎﻫ ﺖ ﯾ ﯽ ﻪﮐ ﺮﺘﮐﺎﺑ نآرد ي
ﺪﺷر
و هدﺮﮑﻧ ﻐﺗ ﯿﯿ ﺮ ﺤﻣ ﻪﺑ ار دﻮﺑ ﻪﺘﻓﺮﮕﻧ ترﻮﺻ ﮓﻧر ﯿ
ﻫ ﺮﻟﻮﻣ ﻂ ﯿ نﻮﺘﻨ
رﺎﮔآ ) نﺎﻤﻟآ كﺮﻣ (
زا ﺲﭘو ﻞﻘﺘﻨﻣ 24
رد نوآ ﺖﻋﺎﺳ ﺎﻣد
ي 37
ﺘﻧﺎﺳ ﻪﺟرد ﯽ
ﻠﭘ ،داﺮﮔ ﯿ زا ﺎﻫ ﺖ ﺮﻈﻧ
ﺮﺘﮐﺎﺑ ﺪﺷر ي ﺳرﺮﺑ ﯽ دﺮﮔ ﯾ ﺪ .
ﺘﻈﻠﻏ ﯽ ﺎﻫ لﻮﻠﺳ ﻪﮐ هرﺎﺼﻋ زا ي
ﻣ ﯿ ﺑوﺮﮑ ﯽ ناﻮﻨﻋ ﻪﺑ ،ﺪﻫد ﺶﻫﺎﮐ ار
ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد MBC ﺪﺷ
] 12 [.
3 - ﺎﺘﻧ ﯾ ﺚﺤﺑ و ﺞ
ﺴﮐا هرذﻮﻧﺎﻧ ﯿ
ور ﺪ ي اراد ي ﺮﺘﮐﺎﺑ ﺪﺿ ﺮﺛا ﯾﺎﯾ
ﯽ ور ﺮﺑ ي ﺮﺘﮐﺎﺑ ي ﺎﻫ ي
ﻔﻨﻣ مﺮﮔ و ﺖﺒﺜﻣ مﺮﮔ ﯽ
ﺮﺘﮐﺎﺑ و هدﻮﺑ ي
ﺎﻫ ي ﺳﺎﺴﺣ ﺖﺒﺜﻣ مﺮﮔ ﯿ
ﺖ
ﺮﺗﻻﺎﺑ ي ﺴﮐا هرذﻮﻧﺎﻧ ﻪﺑ ﺖﺒﺴﻧ ﯿ
ورﺪ ي ﺪﻧا ﻪﺘﺷاد ]
13 [ . ﮑﺳﺎﺗ ﯿ و ﻦ
ﻟﺎﻌﻓ نارﺎﮑﻤﻫ ﯿ
ﺘﻧآ ﺖ ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﺘﻧآو ﯽ ﻣﯿ ﺑوﺮﮑ ﯽ هرﺎﺼﻋ ﺎﻫ ي ﻒﻠﺘﺨﻣ
ﺎﻫ گﺮﺑ ي ﺮﺳ ﯾ ﺎﺷﺎﻤﺗ ﺶ ﯾ ﯽ زرا ار ﯾﺑﺎ ﯽ ﺪﻧدﺮﮐ . ﺎﺘﻧ ﯾ ﮐﺎﺣ ﺞ ﯽ ا زا ﯾ ﻦ
ﺑآ هرﺎﺼﻋ ﻪﮐ دﻮﺑ ﯽ
ﺮﺘﮐﺎﺑ ﺪﺿ ﺮﺛا ﯾﺎﯾ
ﯽ ﺮﺑ ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ
ﺖﺳا ﻪﺘﺷاد ]
14 [ . ﻘﺤﺗ ﻖﺒﻃ ﯿ ﻧﺎﻌﻨﮐ تﺎﻘ ﯽ ورﺮﺑ ﺶﻧارﺎﮑﻤﻫ و ي
صاﻮﺧ ﺘﻧآ ﯽ ﺮﺘﮐﺎﺑ ﯾ ﻟﻮﻧﺎﺘﻣ هرﺎﺼﻋ لﺎ ﯽ
ﮔﯿ ﺮﺳ هﺎ ﯾ ﻣ ﻞﻣاﻮﻋﺮﺑ ﺶ ﯿ
ﺑوﺮﮑ ﯽ
اﺬﻏ ﺺﺧﺎﺷ ﯾ
ﯽ ﺎﺘﻧو ﯾ ا ﺞ ﯾ ﺛﺎﺗ ﺶﻫوﮋﭘ ﻦ ﯿ
ﮐﺮﺗﺮ ﯿ ﺮﺳ هرﺎﺼﻋ ﺐ ﯾ
ﺎﺑ ﺶ
ﺑ هرذﻮﻧﺎﻧ ﯿ ﺛﺎﺗ زا ﺮﺘﺸ ﯿ ﺮﻫ ﺮ ﯾ دﻮﺑ ﺎﻬﻧآ زا ﮏ ]
4[
.
3 - 1 - ﺎﺘﻧ ﯾ د رﺎﺸﺘﻧا ﺞ ﯾ
ﮏﺴ هرﺎﺼﻋ هرذ ﻮﻧﺎﻧ
ﻟﻮﻧﺎﺗا ﯽ ﺮﺳ ﯾ ﺶ
2. Minimum bactericidal concentration
ﯾ ﮑ ﯽ زا هدﺎﺳ ﺮﺗ ﯾ ﺮﺗدﺎﻤﺘﻋا ﻞﺑﺎﻗ و ﻦ ﯾ
ﺖﺴﺗ ﻦ ﺎﻫ ي ﻌﺗ ﯿﯿ ﺻﺎﺧ ﻦ ﯿ ﺖ
ﻣ ﺪﺿ ﯿ ﺑوﺮﮑ ﯽ د رﺎﺸﺘﻧا شور ﯾ
ﮏﺴ ﯾ ﺑﺮﮐ شور ﺎ ﯽ
ﻣ ﺮﺋﻮﺑ ﯽ ﺪﺷﺎﺑ .
اﯾ ﻃ رد شور ﻦ ﯽ
ﺎﻫ لﺎﺳ ي دﺎﻤﺘﻣ ي ﻪﻌﻟﺎﻄﻣ درﻮﻣ هدﺮﺘﺴﮔ رﻮﻃ ﻪﺑ
ﺖﺳا ﻪﺘﻓﺮﮔراﺮﻗ .
ارد ﯾ اﺮﺑ ﺖﺴﺗ ﻦ ي
ﻌﺗ ﯿﯿ ﺻﺎﺧ ﻦ ﯿ ﻣ ﺪﺿ ﺖ ﯿ ﺑوﺮﮑ ﯽ
د فاﺮﻃا ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ زا ﯾ
ﻣ هدﺎﻔﺘﺳا ﮏﺴ ﯽ
دﻮﺷ . ﺎﺑ هزاﺪﻧا ﻪﺑ ﻪﺟﻮﺗ
د فاﺮﻃا ﻪﻟﺎﻫ ﯾ
ﮔﺪﻧرادزﺎﺑ ترﺪﻗ ﮏﺴ ﯽ
ﻣ ﯿ ﻧﺎﮔراوﺮﮑ ﯿ درﻮﻣ ﻢﺴ
ﺳرﺮﺑ نﻮﻣزآ ﯽ
ﻣ ﯽ ددﺮﮔ . ﻘﺤﺗ ﯿ رﺎﺟ ﻖ ي ورﺮﺑ ي ﻪﺳ ﺮﺘﮐﺎﺑ ي
ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ (PTCC1431)
) ﺖﺒﺜﻣ مﺮﮔ ( ود و
ﺮﺘﮐﺎﺑ ي ﻔﻨﻣ مﺮﮔ ﯽ ﺮﺘﻧا ﻼﻧﻮﻤﻟﺎﺳ ﯾ
ﺎﮑ (PTCC 1709) و
ﺷﺮﺷا ﯿ ﺎ
ﻠﮐ ﯽ (PTCC 1399) ﺪﺷر مﺪﻋ ﻪﻟﺎﻫو ﺪﺷ مﺎﺠﻧا
اﺮﺑ ي ا ﯾ ﻪﺳ ﻦ
ﺮﺘﮐﺎﺑ ي ﺳرﺮﺑ ﯽ دﺮﮔ ﯾ ﺪ . ﺎﺘﻧ ﯾ ﺖﻈﻠﻏ رد ﻪﮐ داد نﺎﺸﻧ ﺞ ﺎﻫ
ي 200 -
250 - 300 ) ﻣﯿ ﻠ ﯽ مﺮﮔ / ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ ( ﻫ ،راﺮﮑﺗ ﻪﺳ ﺎﺑ ﯿ
مﺪﻋ ﻪﻟﺎﻫ ﻪﻧﻮﮔ ﭻ
اﺮﺑ ﺪﺷر ي دﺮﮕﻧ هﺪﻫﺎﺸﻣ هرﺎﺼﻋ ﯾ
ﻟ ﺪ ﯿ هرذﻮﻧﺎﻧ زا هدﺎﻔﺘﺳا زا ﺲﭘ ﻦﮑ
ﺴﮐا ﯿ ورﺪ ي ﺖﻈﻠﻏ ﺎﺑ 1000ppm
ﻟﻮﻧﺎﺗا هرﺎﺼﻋرد ﯽ
مﺪﻋ ﻪﻟﺎﻫ ،
ﺪﺷ هﺪﻫﺎﺸﻣ ﺪﺷر )
لوﺪﺟ 2 .(
ﺑﯿ ﺮﺘﺸ ﯾ ﻪﺑ طﻮﺑﺮﻣ ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﻦ
ﺮﺘﮐﺎﺑ ي ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ )
23 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( ﺮﺘﻤﮐ و ﯾ ﻪﻟﺎﻫ ﻦ
ﻪﺑ طﻮﺑﺮﻣ ﺪﺷر مﺪﻋ ﺷﺮﺷا
ﯿ ﻠﮐﺎ ) ﯽ 7 ﻣ ﯿﻠ ﯽ ﺮﺘﻣ ( هﺪﻨﻫد نﺎﺸﻧ ﻪﮐ دﻮﺑ
ﮔﺪﻧرادزﺎﺑ ترﺪﻗ ﯽ
ﺑ ﯿ ﺮﺘﮐﺎﺑ رد هرذﻮﻧﺎﻧ ﺮﺘﺸ ي
ﻪﺑ ﺖﺒﺴﻧ ﺖﺒﺜﻣ مﺮﮔ
ﻔﻨﻣ مﺮﮔ عاﻮﻧا ﯽ
دﻮﺑ . ﻨﭽﻤﻫ ﯿ ﺘﻧآ ﻦ ﯽ ﺑ ﯿ ﺗﻮ ﯿ ﻨﭘ ﮏ ﯽ ﺳ ﯿﻠﯿ ﻪﮐ ﻦ ناﻮﻨﻋ ﻪﺑ
ﺮﺘﮔرﺰﺑ ،ﺖﺳا هﺪﺷ ﻪﺘﻓﺮﮔ ﺮﻈﻧ رد ﺖﺒﺜﻣ لﺮﺘﻨﮐ ﯾ
ار ﺪﺷر مﺪﻋ ﻪﻟﺎﻫ ﻦ
ﺮﺘﮐﺎﺑ ﻪﺳﺮﻫرد ي
داد نﺎﺸﻧ دﻮﺧزا .ا
ﯾ ا ﻪﺑ توﺎﻔﺗ ﻦ ﯾ
ﻟد ﻦ ﯿ ﻪﮐ ﺖﺳا ﻞ
ﮐﺮﺗ ﯿ ﻟ تﺎﺒ ﯿﭙﯿ ﺪ ي ﻟﻮﻠﺳ ءﺎﺸﻏ ﯽ
ﺮﺘﮐﺎﺑ ي ا ﺷﺮﺷ ﯿ ﻼﮐﺎ ي ﺑﯿ زا ﺮﺘﺸ
ﮐﺮﺗ ﯿ ﻟ تﺎﺒ ﯿﭙﯿ ﺪ ي ﻟﻮﻠﺳ ءﺎﺸﻏ ﯽ
ﺮﺘﮐﺎﺑ ي ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ
ﺖﺳا ] 15 [.
Table2 Inhibition zones (mm) of different bacteria affected by nanofluid including Eremurus
spectabiils extract and 1000 ppm ZnO nanoparticles
Treatment Nanofluid nanofluid nanofluid
penicillin positive control
DMSO Negative
control
Staphylococcus aureus 23 22 23 33 0
Salmonella enterica 10 10 10 21 0
Escherichia coli 7 7 7 11 0
3 - 2 - ﻌﺗ ﯿﯿ ﻦ و MIC MBC
ﺳرﺮﺑ ﯽ ﺳﻮﻧﺎﻧ ﺮﺛا ﯿ ﻟﻮﻧﺎﺗا هرﺎﺼﻋ لﺎ ﯽ
ﺮﺳ ﯾ ور ﺶ ي ﺮﺘﮐﺎﺑ ﻪﺳ ي
ﻓﺎﺘﺳا ﯿ ﺷﺮﺷا ،سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ﯿ
ﻠﮐ ﺎ ﯽ ﺮﺘﻧآ ﻼﻧﻮﻤﻟﺎﺳ و ﯾ
ﺎﮑ نﺎﺸﻧ ﻣ
ﯽ ﺑ ﻪﮐ ﺪﻫد ﯿ
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻦ ﯽ
ﻪﺳ ﺪﺷر ﺮﺘﮐﺎﺑ
ي توﺎﻔﺗ
رادﺎﻨﻌﻣ ي دراد دﻮﺟو )
لوﺪﺟ 3 .(
Table 3 MIC and MBC of nanofluid including Eremurus spectabiils extract and 1000 ppm ZnO nanoparticles on different bacteria
Bacterial strains nanofluid including Eremurus spectabiils
extract ZnO Ethanol exract of eremurus
MIC MBC MIC MBC
Staphylococcus aureus 12.5 c 25 b 50 100
Salmonella enterica 100 a 100 a 200 400
Escherichia coli 25 b 100 a 50 400
*Data are the average of three plicate. Date with different letters differ significantly (p<0.05).
ﮔﺪﻨﻨﮐرﺎﻬﻣ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
ﺳﻮﻧﺎﻧ ﯿ ور لﺎ ي ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻪﺑ رﻮﮐﺬﻣ
ﺗﺮﺗ ﯿ ﺐ 5/
12 ، 25 و 100 ﻣ ﯿﻠ ﯽ ﻣﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﻌﺗ ﺮﺘ ﯿﯿ ﺪﺷ ﻦ . ﻧﺎﻌﻨﮐ ﯽ و
نارﺎﮑﻤﻫ ) 2015 ( ﮔﺪﻧرادزﺎﺑ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﯽ
اﺮﺑ ار ي ﺮﺘﮐﺎﺑ ي ﺎﻫ ي
ﺷﺮﺷا ﯿ ﻠﮐﺎ ﯽ ﺮﺘﻧاﻼﻧﻮﻤﻟﺎﺳ ، ﯾ
ﻓﺎﺘﺳا و ﺎﮑ ﯿ
سﻮﺋروا سﻮﮐﻮﮐﻮﻠ 50
mg/ml اﺮﺑو ي ﺮﺘﮐﺎﺑ ﺳﺎﺑي ﯿ سﻮﺋﺮﺳ سﻮﻠ 12/5mg/ml
شراﺰﮔ
ﺪﻧدﻮﻤﻧ . ﻨﭽﻤﻫ ﯿ ﮔﺪﻨﺸﮐ ﺖﻈﻠﻏ ﻞﻗاﺪﺣ ﻦ ﯽ
اﺮﺑ ي ﺮﺘﮐﺎﺑ ي ﺎﻫ ي
ﺷﺮﺷا ﯿ ﻠﮐﺎ ﯽ ﺮﺘﻧآﻼﻧﻮﻤﻟﺎﺳ و ﯾ
،ﺎﮑ 400 ﻣ ﯿﻠﯿ ﻣ ﺮﺑ مﺮﮕ ﯿﻠ ﯽ ﻟ ﯿ اﺮﺑ و ﺮﺘ ي
ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻓﺎﺘﺳا ﯿ ﺳﺎﺑو سﻮﺋروا سﻮﮐﻮﮐﻮﻠ ﯿ
سﻮﻠ سﻮﺋﺮﺳ 100
ﻣﯿ ﻠ ﯽ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟ ﯿ ﻌﺗ ﺮﺘ ﯿﯿ ﺪﺷ ﻦ .
شراﺰﮔ ﺎﻫ ﯾ ﯽ زا تاﺮﺛا ﺪﺿ ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ و ﺪﺿ و ﯾ ﺳوﺮ ﯽ ﻮﻧﺎﻧ داﻮﻣ
دﻮﺟو ﻪﮐ اﺮﭼ دراد ﺎﻤﺗ
ﯾ ﻞ ﻻﺎﺑ ﯾ ﯽ ﻪﺑ ﺶﻨﮐاو ﺎﺑ لﻮﮑﻟﻮﻣ ﺎﻫ ي ز ﯾ ﺘﺴ ﯽ
ﺪﻧراد ﺐﺒﺳو ﻏﯿ لﺎﻌﻓﺮ نﺪﺷ ﺎﻬﻧآ ﻣ ﯽ ﺪﻧدﺮﮔ ﺎﻬﻧردو ﯾ ﺖ وﯾ سوﺮ ﯾﺎ
ﺮﺘﮐﺎﺑ ي ار زا ﺑﯿ ﻦ ﻣ ﯽ ﺪﻧﺮﺑ ] 16 [ . ﻧﺎﮑﻣ ﯿ ﻟﺎﻤﺘﺣا ﻢﺴ ﯽ ﻣ ﺪﺿ ﺮﺛا ﯿ ﺑوﺮﮑ ﯽ
ﺴﮐا تارذﻮﻧﺎﻧ ﯿ
ور ﺪ ي ﻣ ﯽ ذ دراﻮﻣ ﺪﻧاﻮﺗ ﯾ
ﺪﺷﺎﺑ ﻞ : 1
( ﺎﻘﻟا ي ﺴﮐا سﺮﺘﺳا ﯿ
ﺗاﺪ ﯿ ﻟد ﻪﺑﻮ ﯿ ﻟﻮﺗ ﻞ ﯿ دار ﺪ ﯾ لﺎﮑ ﺎﻫ ي ﺴﮐا ﯿ نﮋ
،لﺎﻌﻓ ا ﺶﻨﮐاو ﯾ دار ﻦ ﯾ لﺎﮑ ﺎﻫ ي ﺴﮐا ﯿ ﺎﺑ لﺎﻌﻓ نﮋ ﺌﺗوﺮﭘ ،DNA
ﯿ ﻦ ﺎﻫ
ﻟ و ﯿﭙﯿ ﺘﻧ ردو ﺎﻫﺪ ﯿ لﻮﻠﺳ گﺮﻣ ﻪﺠ .
2 ( ﺑ زا ﯿ ارآ ﻦﺘﻓر ﻦ ﯾ
ﻟد ﻪﺑ ﺎﺸﻏ ﺶ ﯿ
ﻊﻤﺠﺗ ﻞ ﺎﺸﻏرد تارذﻮﻧﺎﻧ ي
ﺮﺘﮐﺎﺑ ي ﻨﭽﻤﻫو ﯿ لﻮﻠﺳ نورد رد ﺎﻬﻧآ ﻊﻤﺠﺗ ﻦ
3 ( نﺪﺷدازآ ﯾ
نﻮ ﺎﻫ ي ور ي ﺎﺸﻏ ﻪﺑ لﺎﺼﺗا ﺎﺑ ﻪﮐ ي
ﻣﯿ ﻧﺎﮔراوﺮﮑ ﯿ ﻢﺴ ﻣ ﺪﺿ ﺮﺛا لﺎﻤﻋا ﺐﺒﺳ ﺎﻫ ﯿ
ﺑوﺮﮑ ﯽ ﻣ ﯽ ﺪﻧﻮﺷ
ا ﺎﺑ ﯾ ﻤﺳ لﺎﺣ ﻦ ﯿ
ﺴﮐا تارذ ﻮﻧﺎﻧ ﺖ ﯿ
ور ﺪ ي ﻘﺘﺴﻣ ﯿ نﺪﺷ دراو ﻪﺑ ﺎﻤ
ﻤﻧ هداد ﺖﺒﺴﻧ لﻮﻠﺳ نورد ﻪﺑ ﺎﻬﻧآ ﯽ
دﺰﻧ سﺎﻤﺗ ﻪﮑﻠﺑ دﻮﺷ ﯾ
ﺎﻬﻧآ ﮏ
ﻐﺗ ﺐﺟﻮﻣ لﻮﻠﺳ ﺎﺑ ﯿﯿ
ﺤﻣ رد ﺮ ﯿ ﺮﺘﮐﺎﺑ ﻂ ي اﺰﻓا ﺎﺑ و هﺪﺷ ﯾ
ﻟﻼﺣ ﺶ ﯿ ﺖ
ﺰﻠﻓ ﯾ ﻟﻮﺗ ﺎ ﯿ دار ﺪ ﯾ لﺎﮑ ﺎﻫ ي ﺴﮐا ﯿ ﺎﻬﻧ رد لﺎﻌﻓ نﮋ ﯾ
ﺳآ ﺚﻋﺎﺑ ﺖ ﯿ
ﻪﺑ ﺐ
ﻣ ﺎﺸﻏ ﯽ ﺪﻧﻮﺷ . ﺶﻫوﮋﭘ ﺎﻫ ي ﻧﺎﮑﻣ دﺪﻌﺘﻣ ﯿ
ﻢﺴ ﺎﻫ ي ﻟﺎﻤﺘﺣا ﯽ و ﺶﻨﮐاو
لﺎﻌﻔﻧاو ﻞﻌﻓ ﺎﻫ
ي ﺎﻫ لﻮﮑﻟﻮﻣوﺮﮐﺎﻣ ﺎﺑ داﻮﻣﻮﻧﺎﻧ ي
ﺑ ﯿ ژﻮﻟﻮ ﯾ ﮑ ﯽ ا ار ﯾ ﻦ
ﭘ رﻮﻃ ﯿ ﻣ دﺎﻬﻨﺸ ﯽ داﻮﻣ ﻮﻧﺎﻧ ﻪﮐ ﺪﻨﻨﮐ ﯾ
ﺎﻫ نﻮ ﯾ ﯽ ﻣ دازآ ﯽ ﺎﺑ ﻪﮐ ﺪﻨﻨﮐ
ﺗ هوﺮﮔ ﯿ لﻮ ) (-SH ﺗوﺮﭘ ﯿ ﺎﻫ ﻦ ي ﺮﺘﮐﺎﺑ لﻮﻠﺳ ﺢﻄﺳﺮﺑ دﻮﺟﻮﻣ ي
ﺎﻫ
ﻣ ﺶﻨﮐاو ﯽ ﺪﻨﻫد .ا ﯾ ﺒﻗ ﻦ ﯿ ﺗوﺮﭘ ﻞ ﯿ ﻦ ﻟﻮﻠﺳ ءﺎﺸﻏ زا ﺎﻫ ﯽ
ﺮﺘﮐﺎﺑ ي ﻪﺑ
ﺑ ﺖﻤﺳ ﯿ ﮔﺪﻣآﺮﺑ نوﺮ ﯽ
اﺬﻏ داﻮﻣ لﺎﻘﺘﻧا ﺐﺟﻮﻣ و ﻪﺘﺷاد ﯾ
ﯽ زا
دﯾ ﻣ لﻮﻠﺳ هراﻮ ﯽ
ﺪﻧﻮﺷ . ا داﻮﻣﻮﻧﺎﻧ ﯾ ﺌﺗوﺮﭘ ﻦ ﯿ ﻦ ﻏ ار ﺎﻫ ﯿ ،هدﺮﮐ لﺎﻌﻓﺮ
ﺬﭘذﻮﻔﻧ ﯾﺮ ي ﻟﻮﻠﺳ گﺮﻣ ﺚﻋﺎﺑ مﺎﺠﻧاﺮﺳو هداد ﺶﻫﺎﮐ ار ءﺎﺸﻏ ﯽ
ﻣ ﯽ
ﺪﻧﻮﺷ ] 16 [.
ﺴﮐا هرذﻮﻧﺎﻧ درﻮﻣ رد ﯿ
ور ﺪ ي ﻣ ﯽ اراد ﻪﮐ ﺖﻔﮔ ناﻮﺗ ي
ﺮﺛا
ﺮﺘﮐﺎﺑﺪﺿ ﯾﺎﯾ ﯽ ورﺮﺑ ي ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﻔﻨﻣ مﺮﮔو ﺖﺒﺜﻣ مﺮﮔ ﯽ
رد و هدﻮﺑ
اﯾ ﻣ ﻦ ﯿ ﺳﺎﺴﺣ ﺖﺒﺜﻣ مﺮﮔ عاﻮﻧا نﺎ ﯿ
ﺮﺗﻻﺎﺑ ﺖ ي ﺮﺘﮐﺎﺑ ﻪﺑ ﺖﺒﺴﻧ ي
ﺎﻫ ي
مﺮﮔ ﻔﻨﻣ ﯽ ﻟﺪﺑ ﻪﮐ ﻪﺘﺷاد ﯿ
د دﻮﺟو ﻞ ﯾ
ﺟرﺎﺧ هراﻮ ﯽ
ﺮﺘﮐﺎﺑ رد ي ﺎﻫ ي
ﻔﻨﻣ مﺮﮔ ﯽ ﺖﺳا . ﺧﺮﺑ ﺮﺑﺎﻨﺑ ﯽ د نﺪﺷ خارﻮﺳ تﺎﻌﻟﺎﻄﻣ ﯾ
ﺮﺘﮐﺎﺑ هراﻮ ي
ﺳو ﻪﺑ ﯿ ﺴﮐا تارذ ﻮﻧﺎﻧ ﻪﻠ ﯿ
ﺰﻠﻓ ﺪ ي لﻮﻠﺳ نورد ﻪﺑ تارذﻮﻧﺎﻧ دورو و
ﻣ ﯽ ﻣﺪﺿ ﺮﺛا لﺎﻤﻋا رد ﺪﻧاﻮﺗ ﯿ
ﺑوﺮﮑ ﯽ ﺪﺷﺎﺑ ﻪﺘﺷاد ﺶﻘﻧ ]
17 [.
ﺎﺘﻧ ﯾ نﺎﺸﻧ هﺪﺷ مﺎﺠﻧا ﺶﻫوﮋﭘ زا ﻞﺻﺎﺣ ﺞ ﺮﺘﮐﺎﺑ داد
ي ﺖﺒﺜﻣ مﺮﮔ
) ﻓﺎﺘﺳا ﯿ سﻮﺋروا سﻮﮐﻮﮐﻮﻠ (
ﺮﺘﮐﺎﺑ ﻪﺑ ﺖﺒﺴﻧ ي
ﺎﻫ ي ﻔﻨﻣ مﺮﮔ ﯽ
) ﻼﻧﻮﻤﻟﺎﺳ ﺮﺘﻧا ﯾ ا و ﺎﮑ ﺷﺮﺷ ﯿ ﻠﮐﺎ ﯽ ( ﺳﺎﺴﺣ ﯿ ﺑ ﺖ ﯿ ﺮﺘﺸ ي ﻪﺑ ﺖﺒﺴﻧ
ﺳﻮﻧﺎﻧ ﯿ وﺎﺣ لﺎ ي هرﺎﺼﻋ ﮔﯿ ﺮﺳ هﺎ ﯾ داد نﺎﺸﻧ ﺶ )
لوﺪﺟ 3 .(
مﺎﻤﺗ
ﻮﺳ ﯾ ﺎﻫ ﻪ ي ﻣ ﯿ ﺑوﺮﮑ ﯽ ﺮﺘﻤﮐ ﺖﻣوﺎﻘﻣ نﻮﻣزآ درﻮﻣ ي
ﻮﻧﺎﻧ ﺮﺑاﺮﺑ رد
ﺳ ﯿ ﺎﻘﻣ رد لﺎ ﯾ ،ﺪﻨﺘﺷاد هرﺎﺼﻋ ﺎﺑ ﻪﺴ اﺮﺑﺎﻨﺑ
ﯾ نﺎﻣﺰﻤﻫ هدﺎﻔﺘﺳا ﻦ ﻮﻧﺎﻧ زا
هرذ ﺎﺑ ZnO ﯾ ﮑ ﯽ زا ﻆﻓﺎﺤﻣ ﺎﻫ ي اﺬﻏ ﯾ ﯽ ﺪﻨﻧﺎﻣ ﺲﻧﺎﺳا ﺎﻫ ي ﮔ ﯿ ﻫﺎ ﯽ
ﻣ ﯽ ناﻮﻨﻋ ﻪﺑ ﺪﻧاﻮﺗ ﯾ
ﮏ ور ﯾ دﺮﮑ ﺮﺛﺆﻣ رد اﺰﻓا ﯾ ﺻﺎﺧ ﺶ ﯿ ﺘﻧآ ﺖ ﯽ
ﺮﺘﮐﺎﺑ ﯾﺎﯾ ﯽ ﺲﻧﺎﺳا و هرﺎﺼﻋ ﺎﻫ
ي ﮔ ﯿ ﻫﺎ ﯽ آرﺎﻤﺷ ﻪﺑ ﯾ ﺪ .
5 - ﻊﺑﺎﻨﻣ
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Antimicrobial Effects of Nano-fluids Based on Eremurus spectabiils Extract and Zinc Oxide Nanoparticles
Tavakkol, M. 1, Mohamadi Sani, A.2, Farahbakhsh, I.1
1. Department of Food Science & Technology, Quchan Branch, Islamic Azad University, Quchan, Iran 2. Young Researchers and Elite Club, Quchan Branch, Islamic Azad University, Quchan, Iran
(Received: 2016/06/15 Accepted: 2017/07/11)
This study aimed to evaluate the antimicrobial effect of nano-fluids based on Eremurus spectabiils extracts against Staphilococcus aureus, Escherichia coli and Salmonella enterica in in-vitro condition.
The extract was obtained by maceration. Disc diffusion method was used for determining the antibacterial activity of the extract at three concentrations: 200, 250, 300 mg/ml and zinc oxide nanoparticles at 1000 ppm. Results showed no inhibition zones for the alone extract while ZnO nanoparticles led to clear zone up to 23mm. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was determined using micro broth dilution method. The highest MIC and MBC was gained for the extract containing ZnO nanoparticles respectively at 12.5 and 25 mg/ml for Staphilococcus aureus. According to the findings, although the ethanolic extract had no inhibitory activity, yet addition of nanoparticles improved the antimicrobial activity which enables the extract to be used as a natural preservative in food and pharmaceutical industries.
Keywords: Antimicrobial activity, Eremurus spectabiils, Zinc oxide
Corresponding Author E-Mail Address: [email protected]
