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Journal of Entomological Society of Iran 2017, 37 (3),293304

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Biochemical properties of digestive proteases of melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)

Moloud Gholamzadeh-Chitgar^1&*, Mohammad Ghadamyari² & Rohangiz Ghanbarinezhad² 1- Plant Protection Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran & 2-Department of Plant Protection, Faculty of Agricultural Science, University of Guilan, Rasht, Iran.

*Corresponding author, Email: b_gh.chitgar60@yahoo.com; m.gholamzadeh@areeo.ac.ir

Abstract

Melon ladybird, Epilachna chrysomelina (Fabricius) (Col.: Coccinellidae)is a major pest of cucurbitaceous plants by feeding on leaves at larval and adult stages. In this study, biochemical properties of digestive proteases of the melon ladybird were studied in its alimentary canal Enzyme activity in the digestive systems of 2^nd, 3^rd and 4^th larval instars and adults showed that the highest specific enzyme activities occurred in 3^rd instar larvae while no significant differences observed in adults.The optimal pH and temperature for protease activity in alimentary canal were found 5 and 30°C, respectively. The inhibitory effect of EDTA, TLCK, TPCK, PMSF and Iodoacetate were determined 44.32, 26.05, 22.27, 19.04 and 18.93% in the gut of E. chrysomelina, respectively. With respect to the highest inhibition rate in proteolytic activity caused by Iodoacetate, cysteine proteinases are considered as the main proteases in the midgut of the melon ladybird. Zymogram pattern in the native gel indicated that the protease enzyme had one isoform in the alimentary canal of the pest.

Key Words: melonladybird, inhibitor, protease Received: 9 March 2017, Accepted: 18 September 2017

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