In vitro Anti-Trichomonas Effects of the Essential Oil and Nano-emulsion of Rosmarinus officinalis on Trichomonase

(1)

₁₁₈

In vitro Anti-Trichomonas Effects of the Essential Oil and Nano-emulsion of Rosmarinus officinalis on Trichomonase

vaginalis

Hajar Ziaei Hezarjaribi¹, Najmeh Nadali²,

Majid Saeidi³, Zohreh Momeni⁴, Masoud Soosaraei⁵,

Mahdi Fakhar¹, Oghol Niaz Jorjani⁶

1 Associate Professor , Department of Parasitology, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran

2 MSc Student in Parasitology, Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

3 Professor, Department of Pharmaceutics, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

4 Assistant Professor, Department of Microbiology, Faculty of Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran

5 PhD Student in Parasitology, Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

6 Assistant Professor, Department of Parasitology, Laboratory Science Research Center, Faculty of Paramedical, Golestan University of Medical Sciences, Gorgan, Iran

(Received Jan 17, 2017, Accepted August 28, 2017)

Abstract

Background and purpose:There is an increasing rate of drug resistance against trichomoniasis and current drugs used in treatment of this disease have many side effects, therefore, attempts have been made to explore alternative drugs favorably from natural sources. The present study aimed at evaluating the anti- Trichomonas effect of essential oil and that of nano-emulsion of Rosmarinus officinalis on the growth of Trichomonas vaginalis (T. vaginalis) in vitro.

Materials and methods: T. vaginalis reference strains were cultured in modified TYM medium. The components of rosemary essential oil were determined by gas chromatography. In addition, cytotoxicity effects of essential oils and nano-emulsions prepared from this plant were evaluated against macrophage cell line (J774.A.1). Serial concentrations of 10, 25, 50, and 100 (μg/mL) were prepared from rosemary essential oil and nano-emulsion. Then growth inhibition rate of the parasites in different concentrations was done in triplicate within 1, 2, and 3 hours.

Results: Rosemary essential oil and nano-emulsion had an inhibitory effect on the growth of Trichomonas and concentration of 100 μg/mL showed growth inhibition effect of 96.1% and 100%

throughout 1,2 and 3 hours, respectively. Additionally, cytotoxicity assay against macrophage revealed no toxicity on the cells at 100 μg/mL.

Conclusion:According to the suitable anti-trichomonas activity of rosemary, this plant could be considered as a good candidate in treatment of Trichomonas infection, after complementary studies on animal model.

Keywords:Trichomonas vaginalis, Rosmarinus officianalis, essential oils, nano-emulsion, in vitro

J Mazandaran Univ Med Sci 2017; 27(153): 118 - 122 (Persian).

[ Downloaded from jmums.mazums.ac.ir on 2022-11-06 ]

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هرود هرﺎﻤﺷ ﻢﺘﻔﻫ و ﺖﺴﯿﺑ 153

ﺮﻬﻣ لﺎﺳ 1396 ) 122 - 118 (

نارﺪﻧزﺎﻣ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻪﻠﺠﻣ

هرﺎﻤﺷ ،ﻢﺘﻔﻫ و ﺖﺴﯿﺑ هرود 153

ﺮﻬﻣ ، 1396 119

ﺮﺗ ﺪﺿ تاﺮﺛا ﯾ

ﺎﻧﻮﻣﻮﮑ ﯽﯾ

ﺴﻟﻮﻣا ﻮﻧﺎﻧ و ﺲﻧﺎﺳا ﯿ

نﻮ ﮔ ﯿ هﺎ رﺎﻣزر ي ﺮﺑ

ﺮﺗ ﯾ سﺎﻧﻮﻣﻮﮑ ژاو

ﯾ ﻟﺎﻨ ﯿ ﺲ اﺮﺷ رد ﯾ

ﻂ ﺎﻣزآ ﯾ ﻫﺎﮕﺸ ﯽ

ﺿ ﺮﺟﺎﻫ ﯿﺎ ﯽﯾ ﺮﺟ راﺰﻫ ﯾﺒ

1ﯽ

ﻠﻋدﺎﻧ ﻪﻤﺠﻧ ﯽ 2

ﺠﻣ ﯿ ﺪ ﻌﺳ ﯿ ﺪ ي 3

هﺮﻫز ﻨﻣﻮﻣ ﯽ 4

دﻮﻌﺴﻣ اﺮﺳﻮﺳ ﯽﯾ 5

ﺪﻬﻣ ي

6رﺎﺨﻓ

ﻞﻏوا ﻧﯿ زﺎ ﻧﺎﺟﺮﺟ

7ﯽ

هﺪﯿﮑﭼ

ﻪﻘﺑﺎﺳ :فﺪﻫ و اﺰﻓا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﯾ

ﺶ وراد ﺖﻣوﺎﻘﻣ دراﻮﻣ ﯽﯾ

ﺒﻧﺎﺟ ضراﻮﻋ و ﯽ

ﺷﺎﻧ ﯽ وراد فﺮﺼﻣ زا ﺎﻫ

ي ار ﯾ ﺞ ﺑ رد ﯿ رﺎﻤ

يﺳرﺮﺑ فﺪﻫ ﺎﺑ ،ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ اﺬﻟ .ﺖﺳايروﺮﺿﯽ ﯿﻌﺒﻃ ﺎﺸﻨﻣ ﺎﺑهﮋﯾو ﻪﺑ ﻦﯾﺰﮕﯾﺎﺟيوراد ﻦﺘﻓﺎﯾياﺮﺑ شﻼﺗ،ﺲﯾزﺎﯿﻧﻮﻣﻮﮑﯾﺮﺗ ﯽــﺣاﺮﻃﯽﻫﺎﮕــﺸﯾﺎﻣزآﻂﯾاﺮــﺷ ردﺲﯿﻟﺎﻨﯾژاوسﺎﻧﻮﻣﻮﮑﺮﯾﺗ ﺮﺑيرﺎﻣزرهﺎﯿﮔنﻮﯿﺴﻟﻮﻣا ﻮﻧﺎﻧ و ﺲﻧﺎﺳاﯽﯾﺎﻧﻮﻣﻮﮑﯾﺮﺗ ﺪﺿ تاﺮﺛا

ﯽ .ﺪﺷ

شور و داﻮﻣ :ﺎﻫ

ا رد ﯾ ﻦ ﺑﺮﺠﺗ ﻪﻌﻟﺎﻄﻣ ﯽ

اﺪﻧﺎﺘﺳا شﻮﺳ ﺪﺷ مﺎﺠﻧا ﯽﻨﺗ نوﺮﺑ ترﻮﺻ ﻪﺑ ﻪﮐ ﺮﺗ در

ﯾ سﺎﻧﻮﻣﻮﮑ ژاو

ﯾ ﻟﺎــﻨ ﯿ ﺲ

ردياﺮــﺑ و ﺺﺨﺸﻣﯽﻓاﺮﮔ ﻮﺗﺎﻣوﺮﮐ زﺎﮔ ﺎﺑيرﺎﻣزر ﺲﻧﺎﺳا رد دﻮﺟﻮﻣتﺎﺒﯿﮐﺮﺗ .ﺪﺷ هداد ﺖﺸﮐﻪﺘﻓﺎﯾﺮﯿﯿﻐﺗ^TYM ﺖﺸﮐﻂﯿﺤﻣ ﻌﺗ ﯿﯿ ﻦ ﻤﺳ ﯿ ﺖ ﻟﻮﻠﺳ ﯽ

، لﻮﻠﺳ زا ﺎﻫ ي هدر ژﺎﻓوﺮﮐﺎﻣ J774.A.1

زرا .ﺪﺷ هدﺎﻔﺘﺳا ﯾﺑﺎ

ﯽ ﻬﺗ ﺎــﺑ ﻞﮕﻧا ﺪﺷر رﺎﻬﻣ ناﺰﯿﻣ ــﯿ

ﻪ ﺖــﻈﻠﻏ ﺎــﻫ

ي 3 و2 ،1ﯽــﻃيرﺎﻣزرنﻮﯿﺴﻟﻮﻣا ﻮﻧﺎﻧ و ﺲﻧﺎﺳا زاﺮﺘﻟﯿﯽﯿﻠﻣ ﺮﺑ مﺮﮔوﺮﮑﻣﯿ100 ،50 ،25 ،10ﯽ ﯾﻟﺎﺮﺳ راﺮــﮑﺗ رﺎــﺑ ﻪــﺳ ﺎــﺑ ﺖﻋﺎــﺳ

.ﺪﺷ مﺎﺠﻧا ﻪﺘﻓﺎﯾ :ﺎﻫ ﺴﻟﻮﻣا ﻮﻧﺎﻧ و ﺲﻧﺎﺳا ﯿ

نﻮ رﺎﻣزر ي رﺎﻬﻣ ﺮﺛا ي ﺮﺗ ﺪﺷر ﺮﺑ ﯾ سﺎﻧﻮﻣﻮﮑ ﺖﻈﻠﻏ رد و ﺖﺷاد 100

ﻣﯿ وﺮﮑ ﻣ ﺮــﺑ مﺮﮔ ﯿــﻠ ﯽ ﻟﯿ ﺮــﺘ

رد ﻦــﯾا ردنﻮﯿــﺴﻟﻮﻣا ﻮﻧﺎﻧ و ﺲﻧﺎﺳا لﺎﮑﺷاﻦﯿﻨﭼﻢﻫ .داد نﺎﺸﻧ ار ﺪﺷر رﺎﻬﻣ ﺪﺻرد100 و ﺪﺻرد96/1 ﺐﯿﺗﺮﺗ ﻪﺑ ﺖﻋﺎﺳ ﻪﺳ لﻮﻃ ﺖــﻈﻠﻏ

ور ﺮﺑ ي ژﺎﻓوﺮﮐﺎﻣ ﻫ ﺎﻫ ﯿ ﭻ ﻤﺳ ﻪﻧﻮﮔ ﯿﺘ ﯽ ﻪﺘﺷاﺪﻧ ﻧا ﺪ .

ﯾﺮﺗ ﺪﺿ بﻮﻠﻄﻣ تاﺮﺛا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ:جﺎﺘﻨﺘﺳا ﺎﻧﻮﻣﻮﮑ

ﯽﯾ ﺴﻟﻮﻣا ﻮﻧﺎﻧ و ﺲﻧﺎﺳا ﯿ

نﻮ ﮔﯿ هﺎ رﺎﻣزر ي اﯾ ﻦ ﮔﯿ

،هﺎ ﻣ ﯽ ﻪﺑ ﺪﻧاﻮﺗ ﺪﻧﺎﮐ ناﻮﻨﻋ ــﯾ

ﺪ .ﺪﺷﺎﺑ حﺮﻄﻣ ﯽﻧاﻮﯿﺣ لﺪﻣ يور ﺮﺑﯽﻧﺎﻣرد تاﺮﺛاﯽ ﯾﺑﺎزرا ﺖﻬﺟﯽﺒﺳﺎﻨﻣ

:يﺪﯿﻠﮐ يﺎﻫ هژاو

ﺮﺗ ﯾ سﺎﻧﻮﻣﻮﮑ ژاو

ﯾ ﻟﺎﻨ ﯿ

،ﺲ رﺎﻣزر ي

، ﺴﻟﻮﻣا ﻮﻧﺎﻧ ،ﺲﻧﺎﺳا ﯿ

،نﻮ ﻨﺗ نوﺮﺑ ﯽ

ﻪﻣﺪﻘﻣ

ﯾﺮﺗ ﻧﻮﻣﻮﮑ ﯿ زﺎ ﯾ ﺲ ژاو ت ﻞﻣﺎﻋ ﺎﺑ ﯾ

ﻟﺎﻨ ﯿ ﺲ ﯾ ﮑ ﯽ ﺎﺷ زا

ﯾﻊــﺴﻨﺟ هار زا ﻪــﻠﻘﺘﻨﻣياﻪــﺘﺧﺎﯾﮏــﺗ يﺎــﻫيرﺎــﻤﺑﯿﻦﯾﺮــﺗ ﯽ ــﻧﻮﻣﻮﮑﯾﺮﺗعﻮﯿﺷ ﻒﻠﺘﺨﻣ تﺎﻌﻟﺎﻄﻣ سﺎﺳا ﺮﺑ .()1ﺖﺳا سﺎ

ژاو ﯾ ﻟﺎﻨ ﯿ ﺲ ژاو ت) ﯾ ﻟﺎﻨ ﯿ ﺲ ا رد ( ــﯾ ناﺮ ــﺑ ادوﺪــﺣ ﯿ ﻦ 37 -

1 ياﺮــﺑﯽﺑﺎــﺨﺘﻧايوراد .( )2دﻮــﺷﯽﻣ دروآﺮﺑ ﺪﺻرد نﺎــﻣرد

اﯾ ﻦ ﺑﯿ رﺎﻤ ي ﻧوﺮﺘﻣ ﯿ لوزاﺪ ﻣ ﯽ ﻟو ﺪﺷﺎﺑ ﯽ ﮐﺎــﺣ تﺎــﺷراﺰﮔ

ﯽ

:لﻮﺌﺴﻣ ﻒﻟﻮﻣ رﺎﺨﻓ يﺪﻬﻣ

- رﺎﺳ ي ﮐﯿ ﺮﺘﻣﻮﻠ 18 ﻫﺎﮕﺸﻧاد ﻊﻤﺘﺠﻣ ،دﺎﺑآ رﺰﺧ هدﺎﺟ ﯽ

ﭘﯿ ﺮﺒﻣﺎ ﮑﺷﺰﭘ هﺪﮑﺸﻧاد ، ﻢﻈﻋا ﯽ

[email protected] Email:

1 . رﺎﯿﺸﻧاد ، ﺳﺎﻨﺷ ﻞﮕﻧا هوﺮﮔ ﯽ

، ﻘﺤﺗ ﺰﮐﺮﻣ ﯿ تﺎﻘ ﺑﯿ ژﻮﻟﻮ ي ﻟﻮﻠﺳ ﯽ ﻟﻮﮑﻟﻮﻣ و ﯽ

، ﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﯽ

رﺎﺳ ،نارﺪﻧزﺎﻣ ي

، اﯾ ناﺮ

ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد،ﯽﮑﺷﺰﭘ هﺪﮑﺸﻧاد،ﯽﯾﻮﺠﺸﻧادتﺎﻘﯿﻘﺤﺗﻪﺘﯿﻤﮐ،ﯽﺳﺎﻨﺷ ﻞﮕﻧا ﺪﺷراﯽﺳﺎﻨﺷرﺎﮐيﻮﺠﺸﻧاد .2 رﺎﺳ ،نارﺪﻧزﺎﻣ

ي

، اﯾ ناﺮ

ناﺮاﯾ،يرﺎﺳ ،نارﺪﻧزﺎﻣﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﯽﯾوراد مﻮﻠﻋتﺎﻘﯿﻘﺤﺗ ﺰﮐﺮﻣ،ﺲﮑﯿﺗﻮﯿﺳﻮﮐﺎﻣرﺎﻓ هوﺮﮔ ،دﺎﺘﺳا .3 4 . دﺎﺘﺳا ﯾ رﺎ ، ﻣ هوﺮﮔ ﯿ وﺮﮑ ﺑﯿ ژﻮﻟﻮ ي

، ﺎﮕﺸﻧاد ،مﻮﻠﻋ هﺪﮑﺸﻧاد ﻣﻼﺳا دازآ ه

ﯽ ،جﺮﮐ ﺪﺣاو ،جﺮﮐ اﯾ ناﺮ

،ﯽﮑﺷﺰﭘ هﺪﮑﺸﻧاد،ﯽﯾﻮﺠﺸﻧادتﺎﻘﯿﻘﺤﺗﻪﺘﯿﻤﮐ،ﯽﺳﺎﻨﺷ ﻞﮕﻧايﺮﺘﮐديﻮﺠﺸﻧاد .5 ﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد

ﯽ رﺎﺳ ،نارﺪﻧزﺎﻣ ي

، اﯾ ناﺮ

ناﺮﯾا ،نﺎﮔﺮﮔ ،نﺎﺘﺴﻠﮔﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧادﯽﻫﺎﮕﺸﯾﺎﻣزآ مﻮﻠﻋتﺎﻘﯿﻘﺤﺗ ﺰﮐﺮﻣ،ﯽﮑﺷﺰﭘﯽﺳﺎﻨﺷ ﻞﮕﻧا هوﺮﮔ ،رﺎﯾدﺎﺘﺳا6.

: ﺖﻓﺎﯾرد ﺦﯾرﺎﺗ 28

/ 10 / 1395 : تﺎﺣﻼﺻا ﺖﻬﺟ عﺎﺟرا ﺦﯾرﺎﺗ 28

/5 / 1396 : ﺐﯾﻮﺼﺗ ﺦﯾرﺎﺗ 6/

6 / 1396

2 / 5

(3)

ﺮﺗ ﺪﺿ تاﺮﺛا ﯾ

ﺎﻧﻮﻣﻮﮑ ﯽﯾ ﺎﻧ و ﺲﻧﺎﺳا ﺴﻟﻮﻣا ﻮﻧ ﯿ نﻮ ﮔﯿ هﺎ رﺎﻣزر ي

120 نارﺪﻧزﺎﻣ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﻪﻠﺠﻣ

هرﺎﻤﺷ ،ﻢﺘﻔﻫ و ﺖﺴﯿﺑ هرود

153 ﺮﻬﻣ ، 1396

وراد ﺖــﻣوﺎﻘﻣ ﺪــﺷر ﻪﺑ ور ﺪﻧور زا ﯽــﯾ

، ﺒﻧﺎــﺟ ضراﻮــﻋ ﯽ

رادرﺎﺑ لوا ﻪﻫﺎﻣ ﻪﺳ رد ﺎﺻﻮﺼﺧ) نآ ناواﺮﻓ ي

نﺎــﻘﻘﺤﻣ (

ﻮﺠﺘﺴﺟ ﻪﺑ ﺎﺗ ﺖﺷاد نآ ﺮﺑ ار ي

وراد ﯽﯾ ﺒﻧﺎﺟ ضراﻮﻋ ﺎﺑ ﯽ

ﺘﻤﮐ نازرا ،ﺮ ﺪــﻧزادﺮﭙﺑ سﺮﺘــﺳد رد و ﺮﺗ )3

لﺎــﺳ رد .( ﺎــﻫ ي

ﺧا ﯿﺮ ﮔ زا هدﺎﻔﺘﺳا ﯿ

نﺎﻫﺎ وراد ﯽﯾ درﻮﻣ راﺮــﻗ نﺎﻘﻘﺤﻣ ﻪﺟﻮﺗ

ﺖﺳا ﻪﺘﻓﺮﮔ )4

ز تﺎﻌﻟﺎﻄﻣ نﻮﻨﮐﺎﺗ .( ﯾ

دﺎ ي تاﺮــﺛا درﻮــﻣ رد

ﺪﺿ ﮔ ﯽﯾﺎﻧﻮﻣﻮﮑﯾﺮﺗ ﯿ

نﺎﻫﺎ ﺖــﺳا ﻪــﺘﻓﺮﮔ مﺎــﺠﻧا ﻒــﻠﺘﺨﻣ )8

، 5 .(

يرﺎﻣزر ) (Rosmarinus officianalis L زا ﺖﺳا ﯽﻫﺎﯿﮔ

ﺘﯾﺪﻣ ﯽﻣﻮــﺑ ﻪــﮐ عﺎــﻨﻌﻧ هداﻮﻧﺎﺧ ﺰــﯿﻧ ناﺮــﯾا رد و هدﻮــﺑ ﻪــﻧاﺮ

ﯽﻣ ﺖﺸﮐ ﻧﺎﻃﺮﺳ ﺪﺿ صاﻮﺧ .دﻮﺷ ﯽ

، ﺑﺎــﻬﺘﻟا ﺪﺿ ﯽ

، ﺪــﺿ

ﯽﺘﻧآ و ﯽﺑوﺮﮑﯿﻣ ﺑﻮــﺧ ﻪــﺑ ﯽــﯾوراد هﺎــﯿﮔ ﻦــﯾا ﯽﻧاﺪﯿﺴﮐا

ﯽ

ﺖﺳا هﺪﺷ ﻪﺘﺧﺎﻨﺷ )

10

، 9 . ( لﺎﺳ رد ﺪﺿ تاﺮﺛا ﻪﺑ ﺮﯿﺧا يﺎﻫ

ﻠﮕﻧا ﯽ لﺎﮑﺷا ﯽﯾﻮﻧﺎﻧ ﮔﯿ نﺎــﻫﺎ وراد ﻒــﻠﺘﺨﻣ ﯽــﯾ

ﻪــﻠﻤﺟ زا

هﮋــﯾو ﻪــﺟﻮﺗ ﺎﻫﻮﯿــﺴﻟﻮﻣاﻮﻧﺎﻧ ﻮﻧﺎــﻧ اﺮــﯾز .ﺖــﺳا هﺪــﺷ يا

نﻮﯿﺴﻟﻮﻣا ﻪﺑ ﺎﻫ ،نﻮﺧ شدﺮﮔ رد وراد ﺖﯿﻫﺎﻣ ﻆﻔﺣ رﻮﻈﻨﻣ

ﻦﺘﺧﺎﺳ دوﺪﺤﻣ ياﺮــﺑ ﯽــﺻﺎﺼﺘﺧا ﻞﺤﻣ ﻪﺑ وراد ﯽﺳﺮﺘﺳد

رﻮﻣ و ﻞﻤﺤﺗ ﻞﺑﺎﻗ راﺪﻘﻣ نﺪﻧﺎﺳر و ﺮﺛا لﺎﻤﻋا نآ رد زﺎﯿﻧ د

ﯽﻣ رﺎﮐ ﻪﺑ ﻞﺤﻣ ﺪﻧور

) 11 .( رد ﺐــﻟﺎﻄﻣ ﻪــﺑ ﻪﺟﻮﺗ ﺎﺑ عﻮﻤﺠﻣ

اﺮﺑ ﺮﺿﺎﺣ ﻪﻌﻟﺎﻄﻣ رد ﺖﻔﮔ ﺶﯿﭘ ي

ﻟوا ﯿ ﻦ ﺪــﺿ تاﺮــﺛا رﺎــﺑ

ژاو ت ﯾ ﻟﺎــﻨ ﯿ ﺲ ــﺴﻟﻮﻣا ﻮﻧﺎــﻧ و ﺲﻧﺎــﺳا لﺎﮑــﺷا ﯿ

نﻮ ﮔ ــﯿ هﺎ

رﺎــﻣزر ي اﺮــﺷ رد ﯾ ﻂ ﺎﻣزآ ﯾ ﻫﺎﮕــﺸ ﯽ ــﻣ ــﺳرﺮﺑ درﻮ ﯽ

راﺮــﻗ

.ﺖﻓﺮﮔ

ﺎﻫ شور و داﻮﻣ

ا رد ﯾ ﻦ ﺑﺮﺠﺗ ﻪﻌﻟﺎﻄﻣ ﯽ

نوﺮﺑ ترﻮﺻ ﻪﺑ ﻪﮐ ،ﺪﺷ مﺎﺠﻧا ﯽﻨﺗ

ژاو ت دراﺪﻧﺎﺘﺳا شﻮﺳ ﯾ

ﻟﺎﻨ ﯿ ﺲ ) ﯽﺳﺮﺘﺳد هرﺎﻤﺷ Ku

551910,1 (

ﺑ زا هﺪﺷ اﺪﺟ ﯿ

رﺎﻤ ﮏﻧﺎﺑ رد دﻮﺟﻮﻣ)

ﻪﻧﻮﻤﻧ ﻞﮕﻧا هﺎﮕﺸﯾﺎﻣزآ ﯽﻠﮕﻧا يﺎﻫ هﺪﮑﺸﻧاد ﯽﺳﺎﻨﺷ

ﯽﮑﺷﺰﭘ ﺤﻣ رد ،( يرﺎﺳ ﯿ

ﻂ ﻧﺰﮔا ﯿ ﮏ (Trypticase-

Yeast-Maltose ) TYM complete ﺷ حﻼﺻا

هﺪ رد و

ﺎﻣد ي 37 ﺪﺷ هداد ژﺎﺳﺎﭘ و ﺖﺸﮐ ﻪﺟرد )

12 . ( ﮔ ﺲﻧﺎﺳا ﯿ هﺎ

رﺎﻣزر ي زﺎﺳوراد ﺖﮐﺮﺷ زا ي

رﺎﺑ ﯾ ﺞ ﻮﻧﺎﻧ و ﺲﻧﺎﺳا

ﺴﻟﻮﻣا ﯿ

،نﻮ زﺎﺳوراد هوﺮﮔ رد ي

ﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﯽ

ﺖﻈﻠﻏ و ﻪﯿﻬﺗ نارﺪﻧزﺎﻣ ﯽﻟﺎﯾﺮﺳ يﺎﻫ

10 ، 25 ، 50 و 100

ﻣﯿ وﺮﮑ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ﺴﻟﻮﻣا ﻮﻧﺎﻧ و ﺲﻧﺎﺳا لﺎﮑﺷا ﯿ

نﻮ

هدﺎﻣآ زﺎﺳ ي ﺴﻟﻮﻣا ﻮﻧﺎﻧ .ﺪﺷ ﯿ

نﻮ ﺎﻫ ي ﺎﺑ بآ رد ﻦﻏور

ﻧزو ﺖﺒﺴﻧ زا هدﺎﻔﺘﺳا ﯽ

1 نﺎﭙﺳا و ﺲﻧﺎﺳا ﺪﺻرد 60

و

ﻮﺗ ﯿﯾ ﻦ 80 ﻧﻮﺳ شور ﻪﺑ ﯿ

ﺳﺎﮑ ﯿ نﻮ ﻬﺗ ﯿﻪ و تﺪﺷ .ﺪﺷ

نﺎﻣز ﺎﻫ ي ﺳرﺮﺑ ﻒﻠﺘﺨﻣ ﯽ

ﺎﻬﻧ رد و ﯾ ﺖ ﻬﺑ ﺖﻟﺎﺣ ﯿ ﻪﻨ ﺮﻈﻧ زا

ﺴﻧﺎﺘﭘ و هزاﺪﻧا ﯿ

ﻞ ﺪﺷ بﺎﺨﺘﻧا ﺎﺗز )

13 اﺰﺟا.( ي ﻋ هﺪﻤ

ﮑﺸﺗ ﯿ ﻞ رﺎﻣزر ﺲﻧﺎﺳا هﺪﻨﻫد ي

ﺳا ﺎﺑ هدﺎﻔﺘ ﻨﮑﺗ زا ﯿ ﮏ زﺎﮔ

ﻓاﺮﮔ ﻮﺗﺎﻣوﺮﮐ ﯽ

ﻌﺗ ﯿﯿ ﻦ دﺮﮔ ﯾﺪ ) 14 ﻮﻧﺎﻧ تارذ هزاﺪﻧا .(

ﺴﻟﻮﻣا ﯿ نﻮ ﻬﺗ ﯿﻪ ﺳو ﻪﺑ هﺪﺷ ﯿ

ﻪﻠ هﺎﮕﺘﺳد DLS (Dynamic

Light Scattering) ﻌﺗ

ﯿﯿ ﻦ دﺮﮔ ﯾ ﺪ هراﺪﻧا ﻪﮐ تارذ

6/

4 98± /7 ﺮﺘﻣﻮﻧﺎﻧ دﻮﺑ .

ﺲــﭘ ﺖــﻈﻠﻏ ﻪــﯿﻬﺗ زا ﻞــﺣاﺮﻣ مﺎــﻤﺗ ﯽﻟﺎﯾﺮــﺳ يﺎــﻫ

ﺎﻣزآ ﯾ ﺶ ــﺿ ﻪــﻌﻟﺎﻄﻣ سﺎــﺳا ﺮﺑ ﯿﺎ

ﯽﯾ و لﺎــﺳ رد نارﺎــﮑﻤﻫ

2017 ﺖــﻓﺮﮔ مﺎــﺠﻧا )

15 اﺮــﺑ .( ي ﺎﻘﻣ ﯾ ﻪــﺴ ﻣ ــﯿ ﮕﻧﺎ ﯿ ﻦ زا ﺎــﻫ

راو نﻮــﻣزآ ــﯾ

ﺲﻧﺎ ــﯾ ﮏ ﻪﺨــﺴﻧ و ﻪــﻓﺮﻃ 22

راﺰــﻓا مﺮــﻧ

ﺪﺷ هدﺎﻔﺘﺳاSPSS .

ﺪﺑ ﯾ ﻦ ژﺎﻓوﺮﮐﺎﻣ زا رﻮﻈﻨﻣ ﺎﻫ

ي هدر ﻟﻮﻠــﺳ ﯽ J774.A.1

ﺠﻨﺳ ﮓﻧر شور ﺎﺑ و هﺪﺷ هدﺎﻔﺘﺳا ﯽ

ﺖﯿﻤــﺳ ناﺰﯿﻣMTT

ـــﻧ و ﺲﻧﺎـــﺳا ﯽﻟﻮﻠـــﺳ ـــﺴﻟﻮﻣا ﻮﻧﺎ

ﯿ نﻮ رﺎـــﻣزر ي ﻪﺒـــﺳﺎﺤﻣ

دﺮﮔ ﯾﺪ ) 16 .(

ﺚﺤﺑ و ﺎﻫ ﻪﺘﻓﺎﯾ

ﺎﺘﻧ ﯾ ﺞ ﺶﯿﺑ ﻪﮐ داد نﺎﺸﻧ ﻪــﺑ ﺪــﺷر رﺎــﻬﻣ ناﺰــﯿﻣ ﻦﯾﺮــﺗ

ﻨﻌﻣ رﻮﻃ ﯽ ﺖﻈﻠﻏ رد ،يراد 100

ﻣﯿ وﺮﮑ ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮــﺘ

ﺗﺮﺗ ﻪــﺑ ــﯿ ﺐ 1 / 96 و 100 دﻮــﺑ مﻮــﺳ ﺖﻋﺎــﺳ رد ﺪــﺻرد

) 0001 /0

>

(P ﻢــﻫ . ــﻨﭼ ﯿ ﻦ ﺎﻘﻣ ﯾ ﻪــﺴ ﺖــﻈﻠﻏ 100 ﻣﯿ وﺮــﮑ

ﻣ ﺮﺑ مﺮﮔ ﯿﻠ ﯽ ﻟﯿ ﺮﺘ ﻧﺎﺳا ــﺴﻟﻮﻣا ﻮﻧﺎــﻧ و ﺲ ﯿ

نﻮ ﮔ ــﯿ هﺎ رﺎــﻣزر ي

رﺎــﻣزر ﺲﻧﺎــﺳا مود و لوا ﺖﻋﺎــﺳ رد ﻪﮐ داد نﺎﺸﻧ ي

ﺮــﺛا

ﺶﯿــﺑ يرﺎﻬﻣ ــﺴﻟﻮﻣا ﻮﻧﺎــﻧ ﻪــﺑ ﺖﺒــﺴﻧ يﺮــﺗ

ﯿ نﻮ رد ﺎــﻣا دراد

ﺴﻟﻮﻣا ﻮﻧﺎﻧ مﻮﺳ ﺖﻋﺎﺳ ﯿ

نﻮ دﻮﺧ يرﺎﻬﻣ ﺮﺛا ﺲﻧﺎﺳا زا ﺮﺘﻬﺑ

ﺧا تﺎﻌﻟﺎﻄﻣ .داد مﺎﺠﻧا ار ﯿﺮ

ﮐﺎﺣ ﯽ ﮔ ﻪــﮐ ﺖﺳا نآ زا ــﯿ

هﺎ

رﺎﻣزر ي ﮐﺮﺗ ﻦﺘﺷاد ﻪﻄﺳاو ﻪﺑ ﯿ

تﺎﺒ ﻠﻨﻓ ﯽ

، ﻣ ﯽ زا رد ﺪــﻧاﻮﺗ

ﺑﯿ ﻦ ﻧﻮﻔﻋ ﻞﻣاﻮﻋ ندﺮﺑ ﯽ

ﺒﻗ زا ــﯿ ﻞ ﺮﺘﮐﺎــﺑ ي ﻞــﮕﻧا و چرﺎــﻗ ،

(4)

153 ﺮﻬﻣ ، 1396

ﻔﻣ ــﯿ ﺪ ﺪــﺷﺎﺑ ) 18

، 17 .( ﻧ ﺮــﺿﺎﺣ ﻪــﻌﻟﺎﻄﻣ رد ــﯿ

،ﺰ ﺮــﺑ هوﻼــﻋ

ﻤﻫا ﻢــﻫ نﺎــﻣز ،ﺖــﻈﻠﻏ ــﯿ

ﺖ اﺰــﻓا ﺎــﺑ و ﺖــﺷاد ﯾ

ﺶ نﺎــﻣز

ﻣ ،تروﺎﺠﻣ ﯿ

ناﺰ ﮔﺪﻨﺸﮐ ﯽ و دﻮــﺑ ﺖــﺒﺜﻣ لﺮــﺘﻨﮐ ﺎــﺑ ﻪﺑﺎﺸﻣ

ﺑﯿ ﺶ ﺮﺗ ﯾ ﻦ ﻪــﻌﻟﺎﻄﻣ ﻪــﺑ ﺖﺒــﺴﻧ ﻪــﮐ دﻮــﺑ مﻮﺳ ﺖﻋﺎﺳ رد ﺮﺛا

ﺿ ﯿﺎ ﯽﯾ لﺎــﺳ رد ﺶﻧارﺎﮑﻤﻫ و 2006

ﻧﻻﻮــﻃ نﺎــﻣز ﯽ

ﺮــﺗ ي

ﻣ ﯽ ﻪــﮐ ﺪﺷﺎﺑ اــﯾ ﻦ ــﻣ ﺮــﻣا ﯽ ﻻد ﺪــﻧاﻮﺗ ــﯾ ﻞ ــﻔﻠﺘﺨﻣ ﯽ ﻪﺘــﺷاد

ﺪﺷﺎﺑ )5 ا ﺖﺳا ﻦﮑﻤﻣ .( ﯾ

ﻦ ﺷﺎﻧ فﻼﺘﺧا ﯽ

ﮔرﺎــﮐ ﻪﺑ زا ﯿﺮ

ي

و هرﺎﺼﻋ ﻒﻠﺘﺨﻣ لﺎﮑﺷا ﯾﺎ

و ﺲﻧﺎــﺳا ــﯾﺎ ــﺴﻟﻮﻣا مﺮــﻓ ﯿ

نﻮ

ﮔﯿ نﺎــﻫﺎ وراد ﯽــﯾ هدروآﺮــﻓ ﻻﺎــﻤﺘﺣا و ﺪــﺷﺎﺑ ﺎــﻫ

ي ﺪــﺿ

ﺮﺗ ﯾ ﺎــﻧﻮﻣﻮﮑ ﯽﯾ ﮔ هﺪــﺷ دازآ ﯿ

نﺎــﻫﺎ هرﺎــﺼﻋ مﺮــﻓ رد قﻮــﻓ

ﻟﻮﻧﺎﺘﻣ ﯽ ﺑﯿ ﺶ ﺮﺗ ﺪﺷﺎﺑ نآ ﺲﻧﺎﺳا مﺮﻓ زا )

19 نﺎــﻤﻫ .( رﻮــﻃ ي

ا رد ﻪﮐ ﯾ ﻦ ﻧ حﺮﻃ ﯿﺰ ﺴﻟﻮﻣا ﻮﻧﺎﻧ ﺮﺛا ﯿ

نﻮ ﮔﯿ هﺎ رﺎــﻣزر ي ﺮــﺘﻬﺑ

رد .دﺮــﮐ ﻞﻤﻋ نآ ﺲﻧﺎﺳا مﺮﻓ زا ــﯾ

ﮏ ــﺳرﺮﺑ ﯽ ﺪــﺿ ﺮــﺛا

ﺮﺗ ﯾ ﺎﻧﻮﻣﻮﮑ ﯽﯾ ﮔ ﯿ هﺎ ﻟﺎﮐوا ﯿ سﻮﺘﭙ زا ﺪﻌﺑ ﺪﺷر زا ﺖﻌﻧﺎﻤﻣ رد

24 د ﻪـــﻌﻟﺎﻄﻣ رد و ﺖﻋﺎـــﺳ ﯾ

ﺮـــﮕ ي زا ﺪـــﻌﺑ 72 ﺎـــﺳ ﺖﻋ

ﺖﺳا هﺪﺷ شراﺰﮔ )

19

، 18 ﻢــﻫ.(

ــﻨﭼ ﯿ ﻦ ﮔ ﻪــﻧﻮﮔ ــﯿ هﺎ درﻮــﻣ ﻄﻣ

ﻪﻌﻟﺎ ﺖﻤﺴﻗ زا هدﺎﻔﺘﺳا و ﺎــﻫ

ي ﮔ ﻒــﻠﺘﺨﻣ ــﯿ

هﺎ ،گﺮــﺑ )

ﻏ و ﻞﮔ ﯿ هﺮ ﻧ ( ﯿﺰ ور ﺮــﺑ ي ﻣ ﺪــﺿ صاﻮــﺧ ﯿ

ــﺑوﺮﮑ ﯽ ﺛﺎــﺗ ﯿﺮ

دراد ) 14

، 10 .( ﻪﺑ رﻮﻃ ﻠﮐ ﯽ ﺎﺠﻧآزا ﯽﯾ تﺎﻌﻟﺎﻄﻣ سﺎﺳا ﺮﺑ ﻪﮐ

ﻣ ﺪــﺿ صاﻮــﺧ ﻒﻠﺘﺨﻣ ﯿ

ــﺑوﺮﮑ ﯽ

، ﻧﺎﻃﺮــﺳ ﺪــﺿ ﯽ

ــﺘﻧآ و ﯽ

ﺴﮐا ﯿ ﻧاﺪ ﯽ رﺎﻣزر ﺲﻧﺎﺳا ي

ﺎﺗ ﯿﯾ ﺪ اﺮﺑﺎــﻨﺑ ،ﺖــﺳا هﺪــﺷ ﯾ

ﻦ ﺎــﺑ

ﺮﺗ ﺪﺿ بﻮﻠﻄﻣ تاﺮﺛا ﻪﺑ ﻪﺟﻮﺗ ﯾ

ﺎﻧﻮﻣﻮﮑ ﯽﯾ ﮔ ــﯿ هﺎ رﺎــﻣزر ي

اﺮﺷ رد ﯾ ﻂ ﺎﻣزآ ﯾ ﻫﺎﮕﺸ ﯽ

، زرا ﯾﺑﺎ ﯽ ﻧﺎﻣرد تاﺮﺛا ﯽ

اﯾ ﻦ ﮔﯿ هﺎ

لﺪﻣ يور ﺮﺑ ﺻﻮﺗ ﯽﻧاﻮﯿﺣ يﺎﻫ

ﯿﻪ ﻣ ﯽ دﻮﺷ ) 17

، 10

، 4 (.

يراﺰﮕﺳﺎﭙﺳ

اﯾ ﻦ ﻘﺤﺗ حﺮــﻃ ﻞــﺻﺎﺣ ﻪــﻟﺎﻘﻣ ﯿ

ﺗﺎــﻘ ﯽ هرﺎﻤــﺷ 1543 و

ﺸﺨﺑ ﯽ ﺎﭘ زا ﯾ نﺎ ﺳﺎﻨﺷرﺎﮐ ﻪﻣﺎﻧ ﯽ

ﺷرا ﻠﻋدﺎــﻧ ﻪــﻤﺠﻧ ﺪ ﯽ

ﺖــﺳا

ﺰﻫ ﻪﮐ ﯾ ﻪﻨ ﺎــﻫ ي ﻘﺤﺗ ﺖــﻧوﺎﻌﻣ ﻂــﺳﻮﺗ نآ ﯿ

تﺎــﻘ روﺎــﻨﻓ و ي

ﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ﯽ

ﻣﺎﺗ نارﺪﻧزﺎﻣ ﯿ

ﻦ .ﺖﺳا هﺪﺷ

References

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2. Ziaei Hezarjaribi H, Fakhar M, Shokri A, Hosseini Teshnizi S,Sadough A,Taghavi M. Trichomonas vaginalis infection among Iranian general population of women: a systematic review and meta-analysis. Parasitol Res 2015;114(4):1291-1300.

3. Forna F, Gulmezoglu AM. Interventions for treating trichomoniasis in women.

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(2):CD000218.

4. Silva NC, Fernandes Júnior A.

Biological properties of medicinal plants: a review of their antimicrobial

activity. J Venom Anim Toxin Incl Trop Dis. 2010;16(3):402-413.

5. Ziaei H, Azadbakht M, Abdollahi F, Shabankhani B. Effect of Methanolic extracts of Artemisia aucheri Boiss, Zataria multiflora Boiss and Myrtus communis L. on Trichomonas vaginalis (In Vitro). J Gorgan Uni Med Sci 2006; 8 (1) :34-38.(persian) 6. Sarkari B, Tadayon H, Askarian S,

Farnia E, Askarian M. In Vitro anti- Trichomonas activity of Freula assafoetida and garlic extracts. J Gorgan Uni Med Sci 2009; 11 (3) :13- 17.(persian)

7. Rafieian M, Hejazi SH, Yusefi HA, Yektaian N, Shirani-Bidabadi L. Effect of Achillea millefolium, Artemisia absinthium & Juglans regia leaves

4 / 5

(5)

ﺮﺗ ﺪﺿ تاﺮﺛا ﯾ

ﺎﻧﻮﻣﻮﮑ ﯽﯾ ﺎﻧ و ﺲﻧﺎﺳا ﺴﻟﻮﻣا ﻮﻧ ﯿ نﻮ ﮔﯿ هﺎ رﺎﻣزر ي

153 ﺮﻬﻣ ، 1396

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