The Effect of Eight Weeks of Voluntary and Forced Endurance Training and Royal Jelly Consumption on NF-κB Gene Expression and Antioxidant Agents in Trimethyltin-Treated Alzheimer's Rats

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The Effect of Eight Weeks of Voluntary and Forced Endurance Training and Royal Jelly Consumption on NF-κB Gene Expression and Antioxidant Agents in

Trimethyltin-Treated Alzheimer's Rats

Awat Hasanloei¹ , Khalid Mohamadzadeh Salamat²^ , Seyed Ali Hosseini³

, Samad Akbarzadeh⁴

1. Department of Physical Education and Sports Sciences, Faculty of Humanities, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. E-mail: [email protected]

2. Corresponding Author, Department of Physical Education and Sports Sciences, Faculty of Humanities, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. E-mail:[email protected]

3. Department of Physical Education and Sport Sciences, Faculty of Humanities, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran. E-mail: [email protected]

4. Department of Clinical Biochemistry, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran. E- mail:[email protected]

Article Info ABSTRACT

Article type:

Research Article

Article history:

Received:

13 February 2022 Received in revised form:

4 July 2022 Accepted:

6 July 2022 Published online:

22 September 2022

Keywords:

alzheimer's, antioxidant, endurance Training, royal jelly, NF-κB.

Introduction:Alzheimer's disease causes hippocampal degeneration, memory loss, and dementia. However, the effect of voluntary and forced endurance training and royal jelly consumption on this disease is not clear.

In this study, the effect of eight weeks of voluntary and forced endurance training and consumption of Royal Jelly (RJ) on NF-κB gene expression and antioxidant factors were investigated in trimethyltin (TMT)-treated Alzheimer’s rats.

Methods: After induction of Alzheimer's disease with TMT, 60 male rats were randomly divided into eight groups, including: first and last week slaughtered Alzheimer's rats control, voluntary training (VT), forced swimming training (ST), sham, voluntary training + RJ consumption (VT + RJ), forced swimming training + RJ Consumption (ST + RJ), and Royal Jelly Consumption (RJ) groups. Also, 12 rats were placed equally in the first and last week slaughtered healthy control groups. The VT and ST groups were placed in a spinning wheel and a special swimming pool for rodents respectively, for eight weeks, and three 60-minute sessions per week using the overload principle. The RJ groups peritoneally received 100 mg/kg/day of royal jelly for eight weeks. One-way ANOVA was used to statistically analyze the values of Malondialdehyde (MDA), Total Antioxidant Capacity (TAC), Beta 2 Microglobulin (B2m), and NF-κB.

Results: TAC gene expression in the hippocampal tissue of ST and VT groups was significant compared with the healthy group (P = 0.02), but MDA gene expression (P=0.165), B2m (P=0.060), and NF-κB (P=0.069) were not significant.

Conclusion: In general, voluntary and forced endurance training and Royal Jelly consumption improve the antioxidant gene expression in the hippocampal tissue of TMT-treated Alzheimer's rats.

Cite this article: Hasanloei A., Mohamadzadeh Salamat Kh., Hosseini SA., & Akbarzadeh, S. The effect of eight weeks of voluntary and forced endurance training and royal jelly consumption on NF-κB gene expression and antioxidant agents in trimethyltin-treated Alzheimer's rats. Journal of Sport Biosciences.2022; 14 (2): 87-102.

DOI: http//doi.org/10.22059/jsb.2022.337245.1505

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ExtendedAbstract

Introduction

Alzheimer's disease causes hippocampal degeneration, memory loss, and dementia. It has been shown that oxidative stress plays an important role in the development of this disease. However, the effect of voluntary and forced training and royal jelly consumption on this disease is not clear. This study aimed to investigate the effect of eight weeks of voluntary and forced endurance training and Royal Jelly (RJ) consumption on NF-κB gene expression and antioxidant factors in trimethyltin (TMT) -treated Alzheimer’s rats.

Methods

In this experimental study, 60 male rats were selected and divided into eight groups, including (1) first week killed Alzheimer’s control, (2) last week killed Alzheimer’s control, (3) rotary wheel Voluntary Training (VT), (4) forced Swimming Training (ST), (5) Sham (Royal Jelly Solvent, Physiological Serum), (6) Voluntary Training + Royal Jelly consumption (VT + RJ), (7) forced Swimming Training + Royal Jelly consumption (ST + RJ), and (8) Royal Jelly consumption (RJ). To investigate the effects of Alzheimer's disease induction on the research variables, 12 rats were placed in the healthy control group killed in the first week and the healthy control group killed in the last week. The VT group was placed on the rotary wheel for eight weeks, and three 60- minute sessions per week. The distance traveled every day was recorded in meters per minute by the odometer device. The ST group performed swimming training in the swimming tub for rodents with the same duration using the principle of overload.

To perform swimming training, rats trained for 5 minutes in the first week, and at the end of this period, the time reached up to 60 minutes. It is worth mentioning that the duration of training for each week was increased by 10 minutes compared with the previous week. The RJ group received 100 mg/kg/day of royal jelly intraperitoneally for eight weeks.

Results

To statistically analyze the values of Malondialdehyde (MDA), Total Antioxidant Capacity (TAC), Beta-2 Microglobulin (B2m), and Nuclear Factor kappa-light-chain- enhancer of activated B cells (NF-κB), one-way ANOVA was used with a significance level of 0.05. The TAC gene expression in the hippocampus tissue of ST and VT groups was significant (P=0.02), but the MDA (P=0.165), B2m (P=0.060), and NF-κB (P=0.069) genes expression were not significant. In general, voluntary and forced endurance training and royal jelly consumption improve the antioxidant gene expression in the hippocampal tissue of Alzheimer's rats treated with TMT. The antioxidant effects of endurance training along with royal jelly consumption are more favorable than endurance training and royal jelly consumption separately.

Conclusion

In general, voluntary and compulsory exercise training and RJ improve the expression of antioxidant genes in the hippocampal tissue of AD rats, and the antioxidant effects of endurance training and exercise training and consumption of royal jelly were more favorable than consumption of royal jelly alone.

Ethical Considerations

Compliance with ethical guidelines: In this research, all the ethical principles of working with animals were carried out according to the Helsinki Convention.

Funding: This research was done without financial support.

Authors’ contribution: The authors contributed equally to the present study

.

Conflict of interest: There is no conflict of interest.

Acknowledgments: We would like to express our gratitude to the Vice-Chancellor of Research affairs of Islamic Azad University, Sanandaj, and Marvdasht Branch, and all persons who have helped us to conduct this research.

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ا ﺮﺑ لﺎﯾور لژ فﺮﺼﻣ ﺎﺑ هاﺮﻤﻫ يزاﻮﻫ ﻦﯾﺮﻤﺗ ﺮﺛ ﺺﺧﺎﺷ

يﺎﻫ ﯽﺘﻧآ ﯽﻧاﺪﯿﺴﮐا ترﻮﺋآ گﺮﺧﺮﺳ ﺖﻓﺎﺑ رد

شﻮﻣ ﯽﻣﻮﺘﮐروا ﯽﯾاﺮﺤﺻ يﺎﻫ ﺪﺷ

ة ﺖﺑﺎﯾد ﻪﺑ ﻼﺘﺒﻣ

ﯽﯾﻮﻠﻨﺴﺣ تاوآ ، 1

ﻟﺎﺧ ﯿ ﺖﻣﻼﺳ هدازﺪﻤﺤﻣ ﺪ

2

، 

ﯽﻠﻋ ﺪﯿﺳ ﯽﻨﯿﺴﺣ

،3

هدازﺮﺒﮐا ﺪﻤﺻ

4

1 . ﺖﯿﺑﺮﺗ هوﺮﮔ ﺪﮑﺸﻧاد ،ﯽﺷزرو مﻮﻠﻋ و ﯽﻧﺪﺑ ة

ناﺮﯾا ،جﺪﻨﻨﺳ ،ﯽﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،جﺪﻨﻨﺳ ﺪﺣاو ،ﯽﻧﺎﺴﻧا مﻮﻠﻋ .

:ﻪﻣﺎﻧﺎﯾار [email protected]

2 . ﺴﻣ هﺪﻨﺴﯾﻮﻧ ﺆ لو هوﺮﮔ ، ﺪﮑﺸﻧاد ،ﯽﺷزرو مﻮﻠﻋ و ﯽﻧﺪﺑ ﺖﯿﺑﺮﺗ ة

،جﺪﻨﻨﺳ ،ﯽﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،جﺪﻨﻨﺳ ﺪﺣاو ،ﯽﻧﺎﺴﻧا مﻮﻠﻋ .ناﺮﯾا

:ﻪﻣﺎﻧﺎﯾار [email protected]

3.

هوﺮﮔ ﺪﮑﺸﻧاد ،ﯽﺷزرو مﻮﻠﻋ و ﯽﻧﺪﺑ ﺖﯿﺑﺮﺗ ة

،ﯽﻧﺎﺴﻧا مﻮﻠﻋ .ناﺮﯾا ،ﺖﺷدوﺮﻣ ،ﺖﺷدوﺮﻣ ﺪﺣاو ،ﯽﻣﻼﺳا دازآ هﺎﮕﺸﻧاد

:ﻪﻣﺎﻧﺎﯾار

@yahoo.com 57

alihoseini_

4 . ﯿﺷﻮﯿﺑ هوﺮﮔ ﺪﮑﺸﻧاد ،ﯽﻨﯿﻟﺎﺑ ﯽﻤ ة

.ناﺮﯾا ،ﺮﻬﺷﻮﺑ ،ﺮﻬﺷﻮﺑ ﯽﮑﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﯽﮑﺷﺰﭘ :ﻪﻣﺎﻧﺎﯾار

[email protected]

ﻪﻟﺎﻘﻣ تﺎﻋﻼﻃا هﺪﯿﮑﭼ

:ﻪﻟﺎﻘﻣ عﻮﻧ ﯽﺸﻫوﮋﭘ ﻪﻟﺎﻘﻣ

رﺎﺗ ﯾ ﺦ رد ﯾ ﺖﻓﺎ : 24 / 11 / 1400

:يﺮﮕﻧزﺎﺑ ﺦﯾرﺎﺗ 13

/ 04 / 1401

رﺎﺗ ﯾ ﺦ ﺬﭘ ﯾ شﺮ : 15 / 04 / 1401

رﺎﺗ ﯾ ﺦ رﺎﺸﺘﻧا : 31 / 06 / 1401

ﺪﯿﻠﮐ هژاو :ﺎﻫ

،ﺮﻤﯾاﺰﻟآ ﺘﻧآ ﯽ ﺴﮐا ﯿ ناﺪ

،

،ﯽﺘﻣﺎﻘﺘﺳا تﺎﻨﯾﺮﻤﺗ

،لﺎﯾور لژ .NF-κB ﻪﻣﺪﻘﻣ

: ﻞﻘﻋ لاوز و ﻪﻈﻓﺎﺣ ﻦﺘﻓر ﺖﺳد زا و ﭗﻣﺎﮐﻮﭙﯿﻫ ﻞﯿﻠﺤﺗ ﺐﺟﻮﻣ ﺮﻤﯾاﺰﻟآ يرﺎﻤﯿﺑ ﯽﻣ

دﻮﺷ تﺎﻨﯾﺮﻤﺗ ﺮﺛا لﺎﺣ ﺮﻫ ﻪﺑ .

يرﺎﻤﯿﺑ ﻦﯾا ﺮﺑ لﺎﯾور لژ فﺮﺼﻣ و يرﺎﺒﺟا و يرﺎﯿﺘﺧا ﯽﺷزرو ﻪﺑ

ﻨﺷور ﯽ ﺺﺨﺸﻣ .ﺖﺴﯿﻧ ﺶﻫوﮋﭘ ﻦﯾا رد ﺮﯿﺛﺄﺗ

ﻪﺘﻔﻫ ﺖﺸﻫ

لﺎﯾور لژ فﺮﺼﻣ و يرﺎﺒﺟا و يرﺎﯿﺘﺧا ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ )

نژ نﺎﯿﺑ ﺮﺑ (RJ NF-κB

ﻞﻣاﻮﻋ و ﯽﺘﻧآ ﯽﻧاﺪﯿﺴﮐا شﻮﻣ يﺎﻫ

يﺮﻤﯾاﺰﻟآ ﯽﯾاﺮﺤﺻ ﺮﺗ ﺎﺑ هﺪﺷ

ﻦﯿﺗ ﻞﯿﺘﻣ ي )

.ﺪﺷ ﯽﺳرﺮﺑ (TMT

:ﺶﻫوﮋﭘ شور ﺎﺑ ﺮﻤﯾاﺰﻟآ يﺎﻘﻟا زا ﺲﭘ

،TMT 60 ﺮﻧ ﯽﯾاﺮﺤﺻ شﻮﻣ ﺮﺳ ﻪﺑ

ترﻮﺻ ﻞﻣﺎﺷ هوﺮﮔ ﺖﺸﻫ ﻪﺑ ﯽﻓدﺎﺼﺗ

يﺮﻤﯾاﺰﻟآ لﺮﺘﻨﮐ ﺪﺷ

ة ﺘﻔﻫ رﺎﺘﺸﮐ ﮥ

،ﺮﺧآ و لوا يرﺎﯿﺘﺧا ﻦﯾﺮﻤﺗ

ﺎﻨﺷ يرﺎﺒﺟا ﻦﯾﺮﻤﺗ ،(VT) + يرﺎﯿﺘﺧا ﻦﯾﺮﻤﺗ ،ﻢﺷ ،(ST)

لﺎﯾور لژ فﺮﺼﻣ (VT+ RJ)

ﻦﯾﺮﻤﺗ ، ) لﺎﯾور لژ فﺮﺼﻣ + يرﺎﺒﺟا ST+ RJ

لﺎﯾور لژ فﺮﺼﻣ و ( )

ﻢﯿﺴﻘﺗ (RJ

.ﺪﻧﺪﺷ 12 رد ﺮﮕﯾد شﻮﻣ ﺮﺳ هوﺮﮔ

يﺎﻫ ﺘﻔﻫ رﺎﺘﺸﮐ ﻢﻟﺎﺳ لﺮﺘﻨﮐ ﮥ

.ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﺮﺧآ و لوا هوﺮﮔ

يﺎﻫ ﻦﯾﺮﻤﺗ وVT ﻪﺑST

تﺪﻣ ﻪﺑ ﻪﺴﻠﺟ ﺮﻫ و ﻪﺘﻔﻫ رد ﻪﺴﻠﺟ ﻪﺳ ،ﻪﺘﻔﻫ ﺖﺸﻫ تﺪﻣ 60

هدﺎﻔﺘﺳا ﺎﺑ ﻪﻘﯿﻗد ﻞﺻا زا

ﻪﻓﺎﺿا رﺎﺑ ﻪﺑ ﺐﯿﺗﺮﺗ و راود خﺮﭼ رد

ﺮﺨﺘﺳا ةﮋﯾو .ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﺎﻨﺷ نﺎﮔﺪﻧﻮﺟ هوﺮﮔ

يﺎﻫ فﺮﺼﻣ ﻪﺘﻔﻫ ﺖﺸﻫ تﺪﻣ ﻪﺑRJ

100mg/kg/day ار

ﻪﺑ ترﻮﺻ

ﺖﻓﺎﯾرد ﯽﻗﺎﻔﺻ ﺮﮐ

ياﺮﺑ .ﺪﻧد ﻪﯾﺰﺠﺗ ﻞﯿﻠﺤﺗو ﺪﯿﺋﺪﻟآ يد نﻮﺌﻟﺎﻣ ﺮﯾدﺎﻘﻣ يرﺎﻣآ )

،(MDA ﯽﺘﻧآ ﺖﻧاﺪﯿﺴﮐا مﺎﺗ ) (، TAC

ﻦﯿﻟﻮﺑﻮﻠﮔوﺮﮑﯿﻣ 2

ﺎﺘﺑ ) و (B2m NF-κB زا ANOVA ﻪﻓﺮﻄﮑﯾ

.ﺪﺷ هدﺎﻔﺘﺳا

ﻪﺘﻓﺎﯾ :ﺎﻫ نژ نﺎﯿﺑ هوﺮﮔ ﭗﻣﺎﮐﻮﭙﯿﻫ ﺖﻓﺎﺑ ردTAC

وST ﻢﻟﺎﺳ هوﺮﮔ ﻪﺑ ﺖﺒﺴﻧVT

ﺎﻨﻌﻣ راد دﻮﺑ ) 02 /0 نﺎﯿﺑ ﺎﻣا ،(P=

نژ )MDA 165 /0 ،(P=

B2m 060) /0 و(P=

NF-κB ) 069 /0 (P=

ﺎﻨﻌﻣ راد .دﻮﺒﻧ

ﻪﺠﯿﺘﻧ :يﺮﯿﮔ ﻪﺑ رﻮﻃ يرﺎﺒﺟا و يرﺎﯿﺘﺧا ﯽﺘﻣﺎﻘﺘﺳا تﺎﻨﯾﺮﻤﺗ ﯽﻠﮐ نژ نﺎﯿﺑ لﺎﯾور لژ فﺮﺼﻣ و

ﺘﻧآ ﯽ ﺴﮐا ﯿ ناﺪ ﺎﻫ ﺖﻓﺎﺑ رد ار

شﻮﻣ ﭗﻣﺎﮐﻮﭙﯿﻫ يﺮﻤﯾاﺰﻟآ يﺎﻫ

ﺎﺑ هﺪﺷ دﻮﺒﻬﺑTMT

ﯽﻣ ﺪﻨﺸﺨﺑ .

دﺎﻨﺘﺳا : ﯽﯾﻮﻠﻨﺴﺣ ، تاوآ

؛ ﺖﻣﻼﺳ هدازﺪﻤﺤﻣ ،

ﺪﯿﻟﺎﺧ ؛ ﯽﻠﻋ ﺪﯿﺳ ،ﯽﻨﯿﺴﺣ

؛ و هدازﺮﺒﮐا ، ﺪﻤﺻ . ﺮﯿﺛﺄﺗ نﺎﯿﺑ ﺮﺑ لﺎﯾور لژ فﺮﺼﻣ و يرﺎﺒﺟا و يرﺎﯿﺘﺧا ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ ﺖﺸﻫ

نژ NF-κB ﻞﻣاﻮﻋ و ﯽﺘﻧآ ﯽﻧاﺪﯿﺴﮐا ﻦﯿﺗ ﻞﯿﺘﻣ يﺮﺗ ﺎﺑ هﺪﺷ يﺮﻤﯾاﺰﻟآ ﯽﯾاﺮﺤﺻ يﺎﻫ شﻮﻣ ﻪﯾﺮﺸﻧ .

ﯽﺷزرو ﯽﺘﺴﯾز مﻮﻠﻋ .

1401 ؛ 14 ) 2(

: 87-102 .

2022.337245.1505

jsb.

/ 10.22059 http//doi.org/

DOI:

ﺎﻧ :ﺮﺷ ،ناﺮﻬﺗ هﺎﮕﺸﻧاد هﺪﮑﺸﻧاد

ﯽﺷزرو مﻮﻠﻋ و

.ﯽﺘﺳرﺪﻨﺗ

.نﺎﮔﺪﻨﺴﯾﻮﻧ©

(4)

90 نﺎﺘﺴﺑﺎﺗ ،مود ةرﺎﻤﺷ ،ﻢﻫدرﺎﻬﭼ ةرود ،ﯽﺷزرو ﯽﺘﺴﯾز مﻮﻠﻋ

1401

ﻪﻣﺪﻘﻣ ﺮﻤﯾاﺰﻟآ يرﺎﻤﯿﺑ )

ﻪﮐ ،(AD

ﻪﺑ رﻮﻃ و ﻪﻈﻓﺎﺣ نداد ﺖﺳد زا ،ﯽﻋﺎﻤﺘﺟا و يﺮﮑﻓ دﺮﮑﻠﻤﻋ ﯽﺠﯾرﺪﺗ ﺖﻣﺎﺧو ﺎﺑ لﻮﻤﻌﻣ ا

،ﺖﺳا هاﺮﻤﻫ ﯽﺘﺧﺎﻨﺷ لﻼﺘﺧ

) ﺪﯿﺋﻮﻠﯿﻣآ ﺎﺘﺑ يﺎﻫﺮﻣﻮﮕﯿﻟا ﯽﻟﻮﻠﺳ ﯽﺒﺼﻋ ﺮﺛا ﺎﺑ ﺺﺨﺸﻣ (Aβ

ﯽﻣ دﻮﺷ )

,

1 2(

ﻪﺑ ﻼﺘﺒﻣ نارﺎﻤﯿﺑ و ﻪﻈﻓﺎﺣ AD

ﺗ ﯽﯾﺎﻧاﻮ يﺎﻫ زا ار دﻮﺧ ﯽﺘﺧﺎﻨﺷ

ﺖﺳد زا رﺎﺘﻓر رد ﺪﯾﺪﺷ تاﺮﯿﯿﻐﺗ ﻖﯾﺮﻃ ﯽﻣ

ﺪﻨﻫد نﺎﻬﺟ ﺖﯿﻌﻤﺟ ﯽﯾﻮﺳ زا . ﻪﺑزور

زور ﺮﺗﺮﯿﭘ ﯽﻣ دﻮﺷ دروآﺮﺑ . ﯽﻣ دﻮﺷ ﻪﮐ 2/

6 ﯽﯾﺎﮑﯾﺮﻣآ نﻮﯿﻠﯿﻣ

65 ﯽﮔﺪﻧز ﺮﻤﯾاﺰﻟآ ﻞﻘﻋ لاوز ﺎﺑ زوﺮﻣا ﺮﺗﻻﺎﺑ و ﻪﻟﺎﺳ ﯽﻣ

ﺪﻨﻨﮐ هدز ﻦﯿﻤﺨﺗ . ﯽﻣ

ﻮﺷ د ﻪﮐ 35 ز نﻮﯿﻠﯿﻣ و دراد دﻮﺟو نﺎﻬﺟ ﺮﺳاﺮﺳ رد ﻞﻘﻋ لاو

ﺶﯿﭘ ﯽﻨﯿﺑ ﯽﻣ دﻮﺷ لﺎﺳ ﺎﺗ 2030 ﻪﺑ 65 ﺪﺳﺮﺑ ﺮﻔﻧ نﻮﯿﻠﯿﻣ )

3(

. ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ ﻪﻠﻤﺟ زا ﯽﻔﻠﺘﺨﻣ ﻞﻣاﻮﻋ ﺢﻄﺳ ،زﺎﻧژورﺪﯿﻫد تﺎﺘﮐﻻ ،

ﯽﺘﻧآ ناﺪﯿﺴﮐا ،

ﺳ ﺶﻫﺎﮐ ،تﺎﻣﺎﺗﻮﻠﮔ ﻪﺑ ﻪﺘﺴﺑاو ﺖﯿﻤﺳ ﺶﯾاﺰﻓا نداد ﺖﺳد زا ﺎﯾ ﺶﻫﺎﮐ ،ﻦﯿﻧﻮﺗوﺮﺳ و ﻦﯿﻟﻮﮐ ﻞﯿﺘﺳا ﺢﻄ

نورﻮﻧ يﺎﻫ زا ﯽﺷﺎﻧ ﺐﯿﺳآ ،ﻦﯿﻣﺎﭘود

لﺎﮑﯾدار يﺎﻫ ﻪﺑ ﺰﻐﻣ ﺖﻓﺎﺑ بﺎﻬﺘﻟا و دازآ ﺪﻧراد ﺶﻘﻧ يرﺎﻤﯿﺑ ﻦﯾا دﺎﺠﯾا رد ﯽﺑﺎﻬﺘﻟا ﻞﻣاﻮﻋ دﻮﺟو ﻞﯿﻟد

) 4(

ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ . ﺲﮑﻌﻨﻣ

ةﺪﻨﻨﮐ مﺪﻋ

ﯽﻣﻮﻤﻋ تاﺮﻫﺎﻈﺗ ﻦﯿﺑ لدﺎﻌﺗ ﻪﻧﻮﮔ

يﺎﻫ نﮋﯿﺴﮐا لﺎﻌﻓ

(ROS)

ياﺮﺑ ﯽﮑﯾژﻮﻟﻮﯿﺑ ﻢﺘﺴﯿﺳ ﮏﯾ ﯽﯾﺎﻧاﻮﺗ و ﻢﺳ

ﯽﯾادز نﺎﺳآ ﻪﻄﺳاو يﺎﻫ ﺎﯾ لﺎﻌﻓ

.ﺖﺳا نآ زا ﯽﺷﺎﻧ ﺐﯿﺳآ ﻢﯿﻣﺮﺗ ﺘﻧآ ﯽ ﺴﮐا ﯿ ناﺪ ﺎﻫ رﺎﻬﻣ ار ﺶﯾﺎﺴﮐا ﻪﮐ ﺪﻨﺘﺴﻫ ﯽﺗﺎﺒﯿﮐﺮﺗ ﯽﻣ

ﺪﻨﻨﮐ ﯽﯾﺎﯿﻤﯿﺷ ﺶﻨﮐاو ﮏﯾ ، ﻣ

ﯽ ﺪﻧاﻮﺗ لﺎﮑﯾدار يﺎﻫ و دازآ ﺶﻨﮐاو ﺎﻫ ي ﺠﻧز ﯿ هﺮ ا ي

ﻪﺑ ﺖﺳا ﻦﮑﻤﻣ ﻪﮐ ﺪﻨﮐ دﺎﺠﯾا لﻮﻠﺳ

يﺎﻫ .ﺪﻧﺎﺳﺮﺑ ﺐﯿﺳآ تادﻮﺟﻮﻣ ﺘﻧآ

ﯽ ﺴﮐا ﯿ ناﺪ ﺎﻫ ﯽﯾ ﻣ ﻦﯿﻣﺎﺘﯾو ﺪﻨﻧﺎ (ﮏﯿﺑرﻮﮑﺳا ﺪﯿﺳا)C

ﻦﯾا ﺖﺳا ﻦﮑﻤﻣ

ﺶﻨﮐاو ﺎﻫ تﺎﻧاﻮﯿﺣ و نﺎﻫﺎﯿﮔ ،ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ ندﺮﮐ لدﺎﻌﺘﻣ ياﺮﺑ .ﺪﻨﻨﮐ رﺎﻬﻣ ار ﺳ

ﯿ ﻢﺘﺴ ﺎﻫ ي ﭘﯿ ﭽ ﯿ هﺪ ا ي زا ﺘﻧآ ﯽ ﺴﮐا ﯿ ناﺪ ﺎﻫ نﻮﯿﺗﺎﺗﻮﻠﮔ ﺪﻨﻧﺎﻣ

ﺖﯿﻓﺮﻇ و زاﺪﯿﺴﮐاﺮﭘ ﯽﺘﻧآ

ﺖﻧاﺪﯿﺴﮐا ) مﺎﺗ

ﻆﻔﺣ ار (TAC

ﯽﻣ ﺪﻨﻨﮐ ) 5(

رد ﻪﮐ ﺪﻧروﺎﺑ ﻦﯾا ﺮﺑ نﺎﻘﻘﺤﻣ . ﺐﯿﺳآ

ﯽﺳﺎﻨﺷ ﺖﯿﻟﺎﻌﻓ ﺪﻨﻧﺎﻣ ﯽﻠﻣاﻮﻋ ،AD

ﺶﯾاﺰﻓا ،ﺎﯿﻠﮔوﺮﮑﯿﻣ ﻦﯾﺎﮐﻮﺘﯾﺎﺳ

ﺶﯾاﺰﻓا ﺎﺑ يرﺪﻨﮐﻮﺘﯿﻣ ﯽﺒﺼﻋ تﻻﻼﺘﺧا ،يﺰﻐﻣ ﯽﻗوﺮﻋ تﻻﻼﺘﺧا ،ﯽﺑﺎﻬﺘﻟا يﺎﻫ ﺎﺘﺑ ﻞﺑﺎﻘﺘﻣ ﺶﯾاﺰﻓا و ROS

) ﺪﯿﺋﻮﻠﯿﻣآ

β

و (A

هﺮﮔ يﺎﻫ يﺮﺒﯿﻓ ﯽﺒﺼﻋ

) NFTs

ﺐﺟﻮﻣ (

نﻮﯿﺳﻼﯾرﻮﻔﺴﻓﺮﭙﯾﺎﻫ ﻦﯿﺌﺗوﺮﭘ

ﻮﺋﺎﺗ ﯽﺴﭘﺎﻨﯿﺳ ﺐﯿﺳآ و1

ﯽﻣ دﻮﺷ ) 6(

.ﺑ ياﺮ لﺎﺜﻣ

لﻮﻠﺳ يﺎﻫ ﻪﻠﻤﺟ زا ،ﻞﻘﻋ لاوز ﻪﺑ ﻼﺘﺒﻣ داﺮﻓا يﺰﻐﻣ ﺐﯿﺳآ زا يﺪﻫاﻮﺷ ،AD

لﺎﮑﯾدار يﺎﻫ نﺎﺸﻧ ار دازآ ﻣ

ﯽ ﺪﻫد ﻪﺑ . ﺮﻈﻧ ﻣ ﯽ ﺪﺳر لﺎﮑﯾدار يﺎﻫ

زآ زا ﻪﮐ ﺪﻧﺰﻐﻣ رد ﺪﯿﺋﻮﻠﯿﻣآ ﻊﻤﺠﺗ ﻞﯾﻻد زا دا وﯾ

ﮔﮋ ﯽ ﺎﻫ ي .ﺖﺳاAD

ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ يﺎﻫﺮﮕﻧﺎﺸﻧ زا ﯽﮑﯾ يد نﻮﺌﻟﺎﻣ

) ﺪﯿﺋﺪﻟآ (MDA

ﺐﯿﮐﺮﺗ ﺎﺑ

ﯽﻤﺳا لﻮﻣﺮﻓ و ﮏﯿﻧﺎﮔرا

CH2 (CHO) 2

ﺖﺳا ؛ ﻊﯾﺎﻣ ﺑ ﯽ ﮓﻧر ﺎﺑ ﯾ ﮏ ناﻮﻨﻋ ﺎﺑ ﻪﮐ ﺖﺳا ﯽﺸﻨﮐاو رﺎﯿﺴﺑ ﺐﯿﮐﺮﺗ خر enol

ﻣ ﯽ ﺪﻫد نﻮﺌﻟﺎﻣ .

يد ﺖﯿﻌﺿو ﺎﺑ طﺎﺒﺗرا رد ﺪﯿﺋﺪﻟآ ﺘﻧآ

ﯽ ﺴﮐا ﯿ ﻧاﺪ ﯽ ﯽﻧﺎﻣز و ﺪﯿﻟﻮﺗ ﻪﮐ دار ﯾ لﺎﮑ ﺎﻫ ي ﺶﯾاﺰﻓا نﺪﺑ رد دازآ ﻣ

ﯽ ﯾ ﺪﺑﺎ ﻪﺑ رﻮﻃ ﺪﯿﻟﻮﺗ ﯽﻌﯿﺒﻃ ﯽﻣ

دﻮﺷ نﻮﻟﺎﻣ .

يد ﺪﯿﺋﺪﻟآ ﻪﻠﻤﺟ زا ﺺﺧﺎﺷ ﺎﻫ ي ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ ﻢﻬﻣ ﺖﺳا

ﻪﮐ هزاﺪﻧا ﮔﯿ ﺮ ي ﯽﻣ دﻮﺷ ) 7(

. قﻼﻃا ﯽﺗﺎﺒﯿﮐﺮﺗ ﻪﺑTAC

ﯽﻣ دﻮﺷ ﻆﻔﺣ ﻪﺑ ردﺎﻗ ﻪﮐ

ﺳ ﯿ ﻢﺘﺴ ﺎﻫ ي ﺮﺑاﺮﺑ رد ﯽﮑﯾژﻮﻟﻮﯿﺑ رﺎﺛآ

ﺮﻀﻣ ﺪﻨﺘﺴﻫ نژوﺮﺘﯿﻧ وROS

ﺐﯿﮐﺮﺗ ﻦﯾا . ﯽﯾﺎﻧاﻮﺗ ﻒﯿﺻﻮﺗ ياﺮﺑ ﻪﮐ ﺖﺳا ﯽﺣﻼﻄﺻا

ﺘﻧآ ﯽ ﺴﮐا ﯿ ناﺪ ﺎﻫ رد

ندﺮﮐ ﺰﯿﻤﺗ ياﺮﺑ ﻒﻠﺘﺨﻣ يﺎﻫاﺬﻏ لﺎﮑﯾدار

يﺎﻫ و نﻮﺧ رد دازآ ﺮﻀﻣ لﻮﻠﺳ

ﺎﻫ ﻪﺑ ﯽﻣ رﺎﮐ دور )7 ( ﻦﯾﺪﻨﭼ . ﻖﯿﻘﺤﺗ ﺖﯿﻟﺎﻌﻓ ﻦﯿﺑ طﺎﺒﺗرا

NF-κB

و ﺑﯿ رﺎﻤ ي ﺎﻫ ي ﺪﻨﻧﺎﻣ ﯽﺒﺼﻋ ) نﻮﺴﻨﯿﮐرﺎﭘ ، AD

) نﻮﺘﮕﻨﯿﺘﻧﺎﻫ و (PD

نﺎﺸﻧ ار (HD

هداد ﺪﻧا ﺎﻬﻧآ . نﺎﯿﺑ مﺪﻋ ﻪﮐ ﺪﻧدﺮﮐ لﺎﻌﻓ

زﺎﺳ ي

NF-κB

رد

لﻮﻠﺳ ﺎﻫ ي ﺪﻧور رد ﯽﺒﺼﻋ ﯽﺘﻈﻓﺎﺤﻣ ﺶﻘﻧ ﯽﺒﺼﻋ ﺮﺨﺗ

ﯾ ﺐ ﺪﻨﻨﮐ ة ﻦﯾا ﺑﯿ رﺎﻤ ي ﺎﻫ دراد ) 1(

. نوﺰﻓا ،ﻦﯾا ﺮﺑ

NF-κB

ﻈﻓﺎﺣ رد ﺖﺳا ﻦﮑﻤﻣ ﮥ

تﺪﻣﺪﻨﻠﺑ زا ﯽﺷﺎﻧ نژ ﺳ

ﯿ لﺎﻨﮕ ﺎﻫ ي ،لﺎﻨﮕﯿﺳ لﺎﻘﺘﻧا رد ﻪﮐ ،ﺪﺷﺎﺑ ﻪﺘﺷاد ﺶﻘﻧ ﯽﺴﭘﺎﻨﯿﺳ ﻞﮑﺷ

ﮔﯿ ﺮ ي و ﻪﻈﻓﺎﺣ زﺎﺴﻟﺪﻣ ي ﯽﻤﻬﻣ ﺶﻘﻧ ﯽﺒﺼﻋ

دراد ) 8(

. ﺮﮕﯾد تﺎﻘﯿﻘﺤﺗ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ مﺎﺠﻧا

ﻪﺘﻓﺮﮔ شﻮﻣ يور ﺘﺨﯾراﺮﺗ يﺎﻫ

ﮥ

TG2576

ﺶﯾاﺰﻓا ،

NF-κB

نﺎﻤﻫ رد زﻮﺘﭘﻮﭘآ و لﻮﻠﺳ

ﺎﻫ ﺖﻓﺎﯾ ﻪﺑ ،ﺪﺷ

ﻦﯾا ﺎﻨﻌﻣ ﻪﮐ لﺎﻌﻓ زﺎﺳ ي

NF-κB

ﺪﻫد ﺶﯾاﺰﻓا ار ﯽﺒﺼﻋ زﻮﺘﭘﻮﭘآ ﺖﺳا ﻦﮑﻤﻣ )

9(

) ﯽﻨﻤﯾا ﺺﻘﻧ ﺮﯿﺴﻣ . ﺮﯿﺴﻣ (IMD

ردTNF

شﻮﻣ ﺎﻫ ﻂﺳﻮﺗ

ﺮﺘﮐﺎﺑ ي ﺎﻫ ي ﯽﺧﺮﺑ و ﯽﻔﻨﻣ ﺳﺎﺑ

ﯿ ﻞ ﺎﻫ ي لﺎﻌﻓ ﺖﺒﺜﻣ ﯽﻣ

دﻮﺷ ﻞﺧاد رﻮﺘﭘادآ ﻦﯿﺌﺗوﺮﭘ ﯽﻨﻤﯾا دﻮﺒﻤﮐ . ﯽﻟﻮﻠﺳ

) ﻨﻣاد ﺎﺑ (Imd

ﮥ ﺎﺑ ﻂﺒﺗﺮﻣ گﺮﻣ

Fas

(dFADD)

ﻪﺒﺷ و زﺎﭙﺳﺎﮐ

ced-3/Nedd2

) گﺮﻣ ﺎﺑ ﻂﺒﺗﺮﻣ

DREDD

ﻪﮐ ( ارImd

ﻣ ﯽ ﺪﻓﺎﮑﺷ نﻮﯿﺳﺎﻨﯿﺘﯿﺋﻮﮐ ﯽﺑﻮﯾ ﺎﺑ ﺲﭙﺳ ،دراد ﻞﻣﺎﻌﺗ ،

لﺎﻌﻓK63

ﯽﻣ دﻮﺷ ﺪﺷر رﻮﺘﮐﺎﻓ نﺪﺷ لﺎﻌﻓ ﻪﺑ ﺮﻣا ﻦﯾا . ﺪﺒﺗ

ﯾ ﻞ ﺪﻨﻨﮐ ة زﺎﻨﯿﮐ ﺎﺘﺑ 1 ) ﺮﺠﻨﻣ (TAK1

ﯽﻣ دﻮﺷ ﺪﻨﻨﮐرﺎﻬﻣ دﻮﺧ ﻪﮐ ة

ﻼﻣD

ﺮﺘﺳﺎﮔﻮﻧ

زﺎﻨﯿﮐ زﺎﻨﯿﮐ ﺲﮑﻠﭙﻤﮐ وβ

γ (dmIKKßγ)

لﺎﻌﻓ ار ﻣ ﯽ ﺪﻨﮐ لﺎﻌﻓ ﻦﯾا . لﺎﻘﺘﻧا ﻪﺑ يزﺎﺳ

NF-kB

ﯽﺴﯾﻮﻧور رﻮﺘﮐﺎﻓ

Rel

ﻪﺘﺴﻫ ﻪﺑ و ﯽﺴﯾﻮﻧور

نژ ) ﯽﺑوﺮﮑﯿﻣﺪﺿ ﺪﯿﺘﭙﭘ يﺎﻫ ﯽﻣ ﺮﺠﻨﻣ ﻦﯿﺴﯾﺮﺘﭙﯾد ﺪﻨﻧﺎﻣ (AMP

رﻮﻣﻮﺗ زوﺮﮑﻧ رﻮﺘﮐﺎﻓ ﺮﯿﺴﻣ .دﻮﺷ

α )α

- رد (TNF M. musculus

ﻂﺳﻮﺗ

1. Tau

(5)

ﺺﺧﺎﺷ ﺮﺑ لﺎﯾور لژ ﺎﺑ هاﺮﻤﻫ يزاﻮﻫ ﻦﯾﺮﻤﺗ ﺮﺛا ﯽﺘﻧآ يﺎﻫ

...گﺮﺧﺮﺳ ﺖﻓﺎﺑ رد ﯽﻧاﺪﯿﺴﮐا

91

) ﺎﻔﻟآ TNF TNF-α

لﺎﻌﻓ ( ﯽﻣ دﻮﺷ ﻪﮐ ﮔﯿ هﺪﻧﺮ ﺎﻫ ي هﺪﻧرﺬﮔ

R1 (TNF-R1)

لﺎﻌﻓ و ﻞﺼﺘﻣ ار ﻣ

ﯽ ﺪﻨﮐ ) هﺪﻧﺮﯿﮔ ﻞﻣﺎﻌﺗ ﻦﯿﺌﺗوﺮﭘ و و (RIP

ﺪﻧﺮﯿﮔ ة ﺖﻣﺪﺧ ﻪﺑ ارTNF

ﻣ ﯽ ﮔﯿ دﺮ رﻮﺘﮐﺎﻓ .

TRAF2

ﻦﯿﺌﺗوﺮﭘ زا لﺎﻌﻓ

هﺪﺷ زﺎﻨﯿﮐ زﺎﻨﯿﮐ نژﻮﺘﯿﻣ ﺎﺑ 3

)

MEKK3

هدﺎﻔﺘﺳا ( ﻣ

ﯽ ﺪﻨﮐ زﺎﻨﯿﮐ ﻦﯾا و

ﺰﯿﻧ ﺑﻮﻧ ﻪﺑ ﮥ دﻮﺧ ﺪﻨﻨﮐرﺎﻬﻣ ة ﮐﯿ زﺎﻨ ﺲﮑﻠﭙﻤﮐ وß γ

(IKKßγ)

لﺎﻌﻓ ار ﻣ ﯽ ﺪﻨﮐ ﺑﺎﺟ ﻪﺑ ﻪﮐ ﻪ ﯽﺴﯾﻮﻧور يﺎﻫرﻮﺘﮐﺎﻓ ﯽﯾﺎﺟ

NF-κB p50

و

RelA

ﺮﺠﻨﻣ ﯽﻣ دﻮﺷ ﺪﻧراد ﺶﻘﻧ بﺎﻬﺘﻟا و ﯽﻨﻤﯾا رد ﻪﮐ نژ ﻦﯾﺪﻨﭼ نﺎﯿﺑ .

، ،ﺎﻬﻧآ زا ﺖﯾﺎﻤﺣ ﺎﺑ

NF-κB

ﺐﺟﻮﻣ رد زﻮﺘﭘﻮﭘآ وﺮﭘ ﺶﯾاﺰﻓا وTNF

iNOS

شﻮﻣ ﭗﻣﺎﮐﻮﭙﯿﻫ رد ﯽﻣ ﻦﯿﺴﮐﻮﺗورﻮﻧ ضﺮﻌﻣ رد يﺎﻫ

.دﻮﺷ

هوﻼﻋ دراﻮﻣ ﺪﯿﻔﻣ ﺶﻘﻧ ﺮﺑ هﺪﺷدﺎﯾ

رد ﺑﯿ رﺎﻤ ي ﺎﻫ ي ﯾﺬﻐﺗ ﺶﻘﻧ ﻪﺑ نﺎﻘﻘﺤﻣ ،ﯽﺒﺼﻋ ﻢﺘﺴﯿﺳ ﮥ

و ﺐﺳﺎﻨﻣ ﺘﻧآ

ﯽ ﺴﮐا ﯿ ناﺪ ﺎﻫ ي هرﺎﺷا ﯽﻌﯿﺒﻃ

ﯽﻣ ﺪﻨﻨﮐ ) لﺎﯾور لژ . (، RJ

ﻪﮐ ﺖﺳا ﻞﺴﻋ رﻮﺒﻧز لﻮﺼﺤﻣ تﺪﻣ

ﺖﺳﺎﻫ ﻪﺑ رﻮﻃ رد ﯽﺘﻨﺳ ﺳ ﯿ ﻢﺘﺴ ﺎﻫ ي هدﺎﻔﺘﺳا ﯽﯾﺎﯿﺳآ و ﯽﯾﺎﭘورا ﯽﮑﺷﺰﭘ ﯽﻣ

دﻮﺷ .

ﻪﺑ ﺮﻈﻧ ﻣ ﯽ ﺪﺳر ﻪﺑ RJ

ﻪﺒﺷ ﺖﯿﻟﺎﻌﻓ ﻞﯿﻟد رد ار ﻦﯿﻟﻮﺴﻧا ﻪﺑ ﺖﻣوﺎﻘﻣ ،ﯽﻨﯿﻟﻮﺴﻧا

ﺑﯿ نارﺎﻤ دﻮﺒﻬﺑ يﺮﻤﯾاﺰﻟآ ﻣ

ﯽ ﺪﺸﺨﺑ هوﻼﻋ . لﺎﯾور لژ ،ﻦﯾا ﺮﺑ

لﺎﮑﯾدار يﺎﻫ ﯽﻧاﻮﯿﺣ لﺪﻣ ﺰﻐﻣ رد ار نﻮﯿﻧآ ﺪﯿﺴﮐا ﺪﻨﻧﺎﻣ دازآ ﯽﺜﻨﺧAD

ﻣ ﯽ ﺪﻨﮐ ﻦﯾا رد . ﻪﻨﯿﻣز نﺎﺸﻧ نﺎﻘﻘﺤﻣ ، هداد

ﺪﻧا ﻪﮐ 100 و 200 ﻣ ﯿﻠ ﯽ مﺮﮔ

نزو مﺮﮔﻮﻠﯿﮐ ﺮﻫ يازا ﻪﺑ نﺪﺑ

تﺪﻣ ﻪﺑRJ

14 ﻦﯿﻓوﺮﺗورﻮﻧ ﺢﻄﺳ ،زور ﺶﯾاﺰﻓا ار ﻪﻈﻓﺎﺣ و ﺎﻫ

ﻣ ﯽ ﺪﻫد دﻮﺒﻬﺑ ار يﺮﯿﮔدﺎﯾ ، ﻣ

ﯽ ﺪﺸﺨﺑ ﺐﯿﺳآ و

شﻮﻣ ﺰﻐﻣ ﺖﻓﺎﺑ رد ﯽﺸﯾﺎﺴﮐا ﯽﯾاﺮﺤﺻ يﺎﻫ

ﺶﻫﺎﮐ ار ﯽﻨﻄﺑ ﻞﺧاد ﯽﻘﯾرﺰﺗ ﻦﯿﺳﻮﺗوزﻮﺘﭘﺮﺘﺳا زا ﯽﺷﺎﻧAD

ﻣ ﯽ ﺪﻫد ) 10 ( .

دﻮﺟو ﺎﺑ ﯽﺳرﺮﺑ تاﺮﯿﺛﺄﺗ ًﻻﺎﻤﺘﺣا ،تﻼﺧاﺪﻣ ﻦﯾا زا ﮏﯾﺮﻫ بﻮﻠﻄﻣ ﻪﻌﻟﺎﻄﻣ

ا ي ﯽﺳرﺮﺑ ياﺮﺑ ﺮﯿﺛﺄﺗ

تﻻﻮﺼﺤﻣ و ﯽﺷزرو تﺎﻨﯾﺮﻤﺗ ﻞﺑﺎﻘﺘﻣ

ﯽﺘﻧآ ﯽﻧاﺪﯿﺴﮐا ﺮﺑ ﯽﻌﯿﺒﻃ

ﺺﺧﺎﺷ يﺎﻫ ﯽﺘﻧآ ﯽﻧاﺪﯿﺴﮐا ﯽﺴﯾﻮﻧور ﻞﻣﺎﻋ و

Nf-kB

رد ﻧ مﺎﺠﻧاAD

ﺘﻓﺮﮕ ﯽﻧﺪﺑ تﺎﻨﯾﺮﻤﺗ ﻞﻣﺎﻌﺗ ،لﺎﺣ ﻦﯾا ﺎﺑ .ﺖﺳا ﻪ

رد ﻪﻨﻣاد ﺎﻫ ي لﺎﯾور لژ زا هدﺎﻔﺘﺳا و ﻒﻠﺘﺨﻣ ﺐﺟﻮﻣ

ﯽﺘﮐﺮﺣ لدﺎﻌﺗ دﻮﺒﻬﺑ )

11 ( نژ نﺎﯿﺑ ﺶﻫﺎﮐ و وAβ

γ-secretase

) 12 ( شﻮﻣ رد يﺎﻫ

ﻪﺑ ﻼﺘﺒﻣ لﺎﯾور لژ ﺎﺑ ﺎﻨﺷ يرﺎﺒﺟا و يرﺎﯿﺘﺧا ﯽﺘﻣﺎﻘﺘﺳا تﺎﻨﯾﺮﻤﺗ ،ﺮﮕﯾد فﺮﻃ زا .ﺪﺷAD

ﺛﺄﺗ ﯿﺮ ي ﺶﯾاﺰﻓا ﺮﺑ وBDNF

ردNGF

شﻮﻣ ﺎﻫ ي

ﻪﺑ ﻼﺘﺒﻣ ﺖﺷاﺪﻧAD

) 1(

. ﻪﺑ ﺮﻫ لﺎﺣ شور ﺎﻫ ي نﺎﻣرد و يﺮﯿﮕﺸﯿﭘ ياﺮﺑ ﯽﻔﻠﺘﺨﻣ .ﺖﺳا هﺪﺷ شراﺰﮔAD

وراد و ﯽﯾاﺬﻏ ﻢﯾژر ﺪﻧﺪﻘﺘﻌﻣ نﺎﺳﺎﻨﺷرﺎﮐ هزوﺮﻣا ﻪﺑ

ﺎﻬﻨﺗ ﯽﯾ ﻪﺑ ﻼﺘﺒﻣ نارﺎﻤﯿﺑ رد تﻻﻼﺘﺧا لﺮﺘﻨﮐ و نﺎﻣرد ياﺮﺑ ﻪﮑﻠﺑ ،ﺖﺴﯿﻧ ﯽﻓﺎﮐ AD

ﻣﺎﻧﺮﺑ ﻪﺑ ﺪﯾﺎﺑ ﺰﯿﻧ شزرو ﮥ

ﻧازور ﮥ نارﺎﻤﯿﺑ دﻮﺷ ﻪﻓﺎﺿا AD

. ،لﺎﺣ ﻦﯾا ﺎﺑ شور

ﺎﻫ ي ﺮﺛﺆﻣ ﻮﻨﻫ رد ز شور ﺎﻫ ي ﺎﻣا ،هﺪﺸﻧ ﺖﻓﺎﯾ يدﺎﻬﻨﺸﯿﭘ تﺎﻘﯿﻘﺤﺗ

شزرو ﻪﮐ ﺖﺳا هداد نﺎﺸﻧ ﺮﯿﺧا ﻪﺑ

ناﻮﻨﻋ ﻠﺧاﺪﻣ ﮏﯾ ﮥ

ﻏ ﯿ ﻤﺟﺎﻬﺗﺮ ﯽ ضراﻮﻋ زا يﺮﯿﮔﻮﻠﺟ رد رد يﺮﯿﮔدﺎﯾ و ﻪﻈﻓﺎﺣ دﻮﺒﻬﺑ وAD

لﺪﻣ ﺎﻫ ي

يرﺎﻤﯿﺑ ﻦﯾا ﯽﻧاﻮﯿﺣ ﺮﺛﺆﻣ

ﺖﺳا ) 13 ( ﻪﺑ . ﺮﻈﻧ ﻣ ﯽ ﺪﺳر (نﺪﯾود و ﺎﻨﺷ ،ﯽﺘﻣوﺎﻘﻣ) ﯽﺷزرو تﺎﻨﯾﺮﻤﺗ ﺐﺟﻮﻣ

ﺖﯿﻓﺮﻇ ﺶﯾاﺰﻓا ﯽﺘﻧآ

ﯽﻧاﺪﯿﺴﮐا

)

،SOD

،TAC

ﻦﯿﻓوﺮﺗورﻮﻧ ،(GTX

،ﺪﯿﺴﮐا ﮏﯾﺮﺘﯿﻧ ،ﯽﺒﺼﻋ ﻢﺘﺴﯿﺳ رد نﻮﺧ نﺎﯾﺮﺟ ،ﯽﻄﯿﺤﻣ يﺎﻫ ﺌﺗوﺮﭘ

ﯿ ﻦ ﺎﻫ ي ﯽﺴﯾﻮﻧور لﻮﺌﺴﻣ ﻪﺘﺴﻫ

ا ي و ،

ﯽﻧاﻮﯿﺣ لﺪﻣ رد يﺮﯿﮔدﺎﯾ و ﻪﻈﻓﺎﺣ ﺶﯾاﺰﻓا ﻦﯿﻨﭽﻤﻫ و ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ ،ﺪﯿﺋﻮﻠﯿﻣآ ﺎﺘﺑ ﺢﻄﺳ ﺶﻫﺎﮐ ﯽﻣAD

دﻮﺷ )

,

13 14 ( ﺞﯾﺎﺘﻧ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ .

هد تﺎﻘﯿﻘﺤﺗ نارﺎﮑﻤﻫ و ﯽﮔرﺰﺑ

ﺛﺄﺗ ﯿ تاﺮ ﻪﺑ ﻼﺘﺒﻣ نارﺎﻤﯿﺑ رد ﺎﻨﺷ يرﺎﺒﺟا و يرﺎﯿﺘﺧا ﯽﻨﯾﺮﻤﺗ شور ود يورAD

46 شﻮﻣ ﺮﺳ اﺮﺤﺻ

ﯽﯾ ﻪﺑ ﻪﮐ

ﻞﻣﺎﺷ ﺮﺳ ﺖﺸﻫ هوﺮﮔ ﺖﻔﻫ 1.

،RJ

2.

،يرﺎﯿﺘﺧا ﻦﯾﺮﻤﺗ 3.

ﺎﻨﺷ يرﺎﺒﺟا ﻦﯾﺮﻤﺗ

، 4.

ﺎﺑ يرﺎﺒﺟا شزﻮﻣآ ،RJ

5.

ﺎﺑ يرﺎﯿﺘﺧا ﻦﯾﺮﻤﺗ ،RJ

6.

و ،لﺮﺘﻨﮐ 7.

ﺴﻠﺟ ﻪﺳ ﻪﮐ ﻢﺷ ﮥ

60 د ﻗﯿ ﻪﻘ ا ي ﺪﻧدﺮﮐ ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ ﺖﺸﻫ تﺪﻣ ﻪﺑ ﻪﺘﻔﻫ رد

، ﺎﻨﺷ يرﺎﺒﺟا و يرﺎﯿﺘﺧا تﺎﻨﯾﺮﻤﺗ ﻪﮐ داد نﺎﺸﻧ

فﺮﺼﻣ ﺎﺑ نﺎﻣﺰﻤﻫ شﻮﻣ رد ﻪﻈﻓﺎﺣ دﻮﺒﻬﺑ ﺮﺑ ﻞﺑﺎﻘﺘﻣ تاﺮﯿﺛﺄﺗRJ

ﻪﺑ ﻼﺘﺒﻣ يﺎﻫ درادAD

) 1(

ﺖﺸﻫ ﺮﺛا نارﺎﮑﻤﻫ و يداﺮﻣ . ) ﻦﯾﺮﻤﺗ ﻪﺘﻔﻫ

ﺞﻨﭘ

شﻮﻣ و نادﺮﮔ خﺮﭼ يور يرﺎﯿﺘﺧا ﺖﯿﻟﺎﻌﻓ (ﻪﺘﻔﻫ رد ﻪﺴﻠﺟ تﺪﻣ ﻪﺑ ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ هوﺮﮔ يﺎﻫ

ﺖﺸﻫ ﻪﺘﻔﻫ ﺮﻫ) ﻪﺘﻔﻫ ﺞﻨﭘ

ﺖﯿﻟﺎﻌﻓ (ﻪﺴﻠﺟ

ﻦﯿﮐﻮﻟﺮﺘﻨﯾا حﻮﻄﺳ ﺮﺑ ﻞﯿﻣدﺮﺗ يور يزاﻮﻫ ﻢﻈﻨﻣ 6

شﻮﻣ رد نوﺮﺘﺳﻮﺘﺴﺗ و رﺪﻨﺳ ﻪﺑ ﻼﺘﺒﻣ ﯽﯾاﺮﺤﺻ يﺎﻫ

و ﯽﻠﭘ ناﺪﻤﺨﺗ م ﻪﺴﯾﺎﻘﻣ ار ﮏﯿﺘﺴﯿﮐ

ﺘﻧ .ﺪﻧدﺮﮐ يﺎﻫرﻮﺘﮐﺎﻓ ﺮﺑ ﯽﺘﻣﺎﻘﺘﺳا و يرﺎﯿﺘﺧا ﻦﯾﺮﻤﺗ ﻪﮐ داد نﺎﺸﻧ ﺞﯾﺎ تر نزو و نوﺮﺘﺳﻮﺘﺴﺗ ،IL-6

ﺎﻫ ﺮﯿﺛﺄﺗ دراد ﯽﻧﺎﺴﮑﯾ )

15 ( مﺎﺠﻧا ﯽﻫﺎﮔ .

تﺪﺷ ﯽﻣ اﺪﯿﭘ يرﺎﺒﺟا ﻞﮑﺷ ﯽﺘﻗو و ﺖﺳا ﻞﮑﺸﻣ نارﺎﻤﯿﺑ ﻦﯾا ياﺮﺑ ﻦﯾﺮﻤﺗ نﻮﮔﺎﻧﻮﮔ يﺎﻫ ﯽﻣ دﺎﺠﯾا ﻦﻣﺰﻣ سﺮﺘﺳا ،ﺪﻨﮐ

و ﺪﻨﮐ ﺐﺟﻮﻣ ﺛﺄﺗ ﯿ تاﺮ

يور ﺮﺑ ﯽﺤﺷﺮﺗ ﯽﻧﻮﻣرﻮﻫ ﺖﻓﺎﺑ

ﺎﻫ ي ﭗﻣﺎﮐﻮﭙﯿﻫ )

,

16 17 ( ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﺞﯾﺎﺘﻧ

رد نارﺎﮑﻤﻫ و شزﻮﭘ تﺎﻘﯿﻘﺤﺗ ﮥﻨﯿﻣز

يﺎﻨﺷ ﻦﻣﺰﻣ سﺮﺘﺳا رﺎﺛآ

ﺮﺑ ﻒﻠﺘﺨﻣ تﺎﺴﻠﺟ داﺪﻌﺗ ﺎﺑ يرﺎﺒﺟا ﻠﺣﺮﻣ

ﮥ زﺎﻓ ﯽﻧﺎﯾﺎﭘ ﺖﻤﺴﻗ و زﺎﻓﺮﺘﻨﯾا 2

ﻦﯿﻟﺎﻣﺮﻓ نﻮﻣزآ رد

، تﺎﺴﻠﺟ داﺪﻌﺗ رد ﻦﻣﺰﻣ يﺎﻨﺷ سﺮﺘﺳا 3

، 5 و

10 ﺮﺑ تﺎﺴﻠﺟ داﺪﻌﺗ ﺶﯾاﺰﻓا و هﺪﺷ درد يﺎﻫرﺎﺘﻓر نﺪﺷ ﯽﻧﻻﻮﻃ ﺐﺒﺳ زور ﺎﻫرﺎﺘﻓر

ي ﻠﺣﺮﻣ رد درد ﮥ

زﺎﻓﺮﺘﻨﯾا ﺮﯿﺛﺄﺗ ﺎﻨﻌﻣ راد ﻪﮐ ﺖﺳا ﻪﺘﺷاد ي

ﻣ ﯽ ﺪﻧاﻮﺗ ﺮﺛا زا ﯽﺷﺎﻧ ﻧﻻﻮﻃ

ﯽ تﺪﻣ ﺮﺑ ﺎﻫرﺎﮐوزﺎﺳ ي

ﺪﺷﺎﺑ ﻪﻠﺣﺮﻣ ﻦﯾا رد درد ﯽﻠﯾﺪﻌﺗ .

رﺎﺑرد تﺎﻋﻼﻃا ﺖﯾدوﺪﺤﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ة

ﻪﺑ شزرو عاﻮﻧا تاﺮﯿﺛﺄﺗ شزرو هﮋﯾو

ﯽﺸﯾﺎﺴﮐا رﺎﺸﻓ و بﺎﻬﺘﻟا ﺮﺑ يزاﻮﻫ يﺎﻫ ﻧﻮﻣرﻮﻫ و

ﯽ ﻪﺑ ﺮﻈﻧ

ﯽﻣ ﯽﻣ و ﺪﻧراد يرﺎﯿﺘﺧا ﻞﮑﺷ ﻪﮐ ﯽﺗﺎﻘﯿﻘﺤﺗ ﻦﯿﻨﭼ مﺎﺠﻧا ﻪﮐ ﺪﺳر ﺟﻮﺗ ﺎﺑ .ﺖﺳا يروﺮﺿ ،ﺪﻧﻮﺷ ﻊﻗاو ﺮﺛﺆﻣ ﺪﻨﻧاﻮﺗ

ﻪﺑ ﻪ اﯾ ﻪﮑﻨ ﻪﺑ ﯽﮐﺪﻧا تﺎﻘﯿﻘﺤﺗ

(6)

92

ﻋ نﺎﺘﺴﺑﺎﺗ ،مود ةرﺎﻤﺷ ،ﻢﻫدرﺎﻬﭼ ةرود ،ﯽﺷزرو ﯽﺘﺴﯾز مﻮﻠ 1401

ﯽﺳرﺮﺑ ﺮﯿﺛﺄﺗ ﻪﺑ ﻼﺘﺒﻣ داﺮﻓا رد ﯽﻧﻮﻣرﻮﻫ و ﯽﺑﺎﻬﺘﻟا يﺎﻫﺮﮐرﺎﻣ ﺮﺑ نادﺮﮔ خﺮﭼ يور يرﺎﯿﺘﺧا تﺎﻨﯾﺮﻤﺗ ﻪﺘﺧادﺮﭘ AD

ﺪﻧا و ﺮﯿﺛﺄﺗ تﺎﻨﯾﺮﻤﺗ ﺎﺑ نآ

ﯽﻣ (يرﺎﺒﺟا) ﯽﺘﻣﺎﻘﺘﺳا ﺿرﺎﻋ ياراد داﺮﻓا ياﺮﺑ ﺪﻧاﻮﺗ

ﮥ ﺪﺷﺎﺑ ﺖﯿﻤﻫا ﺰﺋﺎﺣAD

، ﻦﯾاﺮﺑﺎﻨﺑ تاﺮﯿﺛﺄﺗ ود ﻦﯾا ﻞﺑﺎﻘﺘﻣ ﻪﻠﺧاﺪﻣ

ﯽﺘﻣﺎﻘﺘﺳا تﺎﻨﯾﺮﻤﺗ)

زﻮﻨﻫ (يرﺎﺒﺟا و يرﺎﯿﺘﺧا ﻪﺑ

ﺑﻮﺧ ﯽ ﻪﺋارا ﺐﻟﺎﻄﻣ ﻪﺑ ﺖﯾﺎﻨﻋ ﺎﺑ .ﺖﺳا هﺪﺸﻧ كرد و يرﺎﯿﺘﺧا ﻦﯾﺮﻤﺗ عﻮﻧ ود ﺎﯾآ ﻪﮐ ﺖﺳا ﻦﯾا ﺶﻫوﮋﭘ لاﺆﺳ ،هﺪﺷ

فﺮﺼﻣ ﺎﺑ هاﺮﻤﻫ (يرﺎﺒﺟا) ﯽﺘﻣﺎﻘﺘﺳا ﺮﺑRJ

ﺰﻧآ ﯾ ﻢ ﺎﻫ ي ﯽﺘﻧآ ﯽﻧاﺪﯿﺴﮐا ﯽﺴﯾﻮﻧور رﻮﺘﮐﺎﻓ و

NF-κB

شﻮﻣ ﭗﻣﺎﮐﻮﭙﯿﻫ ﺖﻓﺎﺑ رد ﯽﯾاﺮﺤﺻ يﺎﻫ

زا ﯽﺷﺎﻧAD

ﺮﯿﺛﺄﺗTMT

؟ﺮﯿﺧ ﺎﯾ دراد

شور ﺶﻫوﮋﭘ ﯽﺳﺎﻨﺷ

ﻦﯾا رد ﻖﯿﻘﺤﺗ ،ﯽﺑﺮﺠﺗ 60 ﻢﻟﺎﺳ ناﻮﺟ ﺮﻧ ﯽﯾاﺮﺤﺻ شﻮﻣ

Sprague Dawley

ﻨﻣاد ﺎﺑ ﮥ ﯽﻨﺳ 8 - 10 ﻨﻣاد و (غﻮﻠﺑ ﻦﺳ) ﻪﺘﻔﻫ ﮥ

نزو 200 ﺎﺗ

220 رد ﺎﻬﻧآ .ﺪﻧﻮﺷ رﺎﮔزﺎﺳ دراﺪﻧﺎﺘﺳا ﻂﯾاﺮﺷ رد ﺎﺗ ﺪﻧﺪﺷ ﻞﻘﺘﻨﻣ تﺎﻧاﻮﯿﺣ هﺎﮕﺸﯾﺎﻣزآ ﻪﺑ ﻪﺘﻔﻫ ﮏﯾ تﺪﻣ ﻪﺑ و هﺪﺷ ﻪﯿﻬﺗ مﺮﮔ ﺲﻔﻗ

ﺎﻫ ي فﺎﻔﺷ

ﯽﻠﭘ تﺎﻨﺑﺮﮐ فﺎﻄﻌﻧا ﺬﭘ ﯾﺮ ) بﻮﻠﻄﻣ يﺎﻣد ، 20

ﺎﺗ 24 ﺟرد ﮥ ﺘﻧﺎﺳ ﯽ داﺮﮔ دوﺪﺣ ﯽﺒﺴﻧ ﺖﺑﻮﻃر ،(

55 ﺎﺗ 65 ﺧﺮﭼ ،ﺪﺻرد ﮥ

ﯽﯾﺎﻨﺷور و ﯽﮑﯾرﺎﺗ 12

ﻋﺎﺳ .ﺪﻧﺪﺷ يراﺪﻬﮕﻧ اﺬﻏ و بآ ﻪﺑ دازآ ﯽﺳﺮﺘﺳد و ﻪﺘ نﺎﯾﺎﺷ

ﻪﮐ ﺖﺳا ﺮﮐذ ﯽﻣﺎﻤﺗ

ﻦﯾا رد تﺎﻧاﻮﯿﺣ ﺎﺑ رﺎﮐ ﯽﻗﻼﺧا لﻮﺻا ﻖﯿﻘﺤﺗ

ترﺎﻈﻧ ﺖﺤﺗ

ﺘﯿﻤﮐ ﮥ .ﺖﺳا هدﻮﺑ ﺖﺷدوﺮﻣ ﺪﺣاو ﯽﻣﻼﺳا دازآ هﺎﮕﺸﻧاد ،ﯽﻫﺎﮕﺸﯾﺎﻣزآ تﺎﻧاﻮﯿﺣ ﺎﺑ رﺎﮐ قﻼﺧا ﺎﺘﺑ ﺶﯾاﺰﻓا ﺪﻫﺎﺷ ﻦﯿﺘﻠﯿﺘﻣ يﺮﺗ ﻖﯾرﺰﺗ زا ﺲﭘ

رد ﻮﺋﺎﺗ و ﺪﯿﺋﻮﻠﯿﻣآ رد ﯽﻋﺎﺨﻧ يﺰﻐﻣ ﻊﯾﺎﻣ يﺎﻤﺳﻼﭘ

شﻮﻣ ﺎﻫ ﯽﻠﺻا ﻞﻣاﻮﻋ زا ﻪﮐ ﻢﯾﺪﺷ ﻪﺑ

دﻮﺟو نﺪﻣآ ردAD

شﻮﻣ ﺖﺳﺎﻫ . ﻦﯿﻨﭽﻤﻫ ﻪﺑ رﻮﻈﻨﻣ

نﺪﺷ يﺮﻤﯾاﺰﻟآ زا نﺎﻨﯿﻤﻃا شﻮﻣ

ﺎﻫ ﮏﯾ شﻮﻣ رد يﺮﻤﯾاﺰﻟآ ﯽﻨﯿﻟﺎﺑ ﻢﺋﻼﻋ يﺮﺳ ﺪﻨﻧﺎﻣ ﯽﯾاﺮﺤﺻ يﺎﻫ

1.

ﻪﺸﻋر ﺎﻫ ي ﯽﻧﻼﻀﻋ ،1

2.

يﺎﻣد ﺶﯾاﺰﻓا

،نﺪﺑ 3.

،ﯽﻨﯿﺑ و ﻢﺸﭼ زا يﺰﯾﺮﻧﻮﺧ 4.

،عﻮﻬﺗ ﺖﻟﺎﺣ 5.

و ﺞﻨﺸﺗ 6.

ﭘ ﯿ ﭻ بﺎﺗو ﺎﻫ ي هﺪﻫﺎﺸﻣ ﯽﻣد ﺷ

ﺪ ) 18 ( . ﻪﺑ ،ﻢﺘﺸﻫ زور رد ﺲﭙﺳ 48

شﻮﻣ

ﻪﺑ ترﻮﺻ ﻞﺧاد ﯽﻗﺎﻔﺻ 100 ﻣ ﯿﻠ ﯽ مﺮﮔ ) ﻦﯿﺗ ﻞﯿﺘﻣ يﺮﺗ ﺪﺷ ﻖﯾرﺰﺗ نﺪﺑ نزو مﺮﮔﻮﻠﯿﮐ يازا ﻪﺑ (TMT

) 19 ( زا ﺲﭘ و رﺎﻬﭼ

زا نﺎﻨﯿﻤﻃا زور

ﺮﯿﺛﺄﺗ شﻮﻣ ،ﭗﻣﺎﮐﻮﭙﯿﻫ يور ﺮﺑ ﻞﻣﺎﮐ ياراد يﺎﻫ

ﻪﺑAD

رﻮﻃ هوﺮﮔ ﺖﺸﻫ ﻪﺑ ﯽﻓدﺎﺼﺗ ﺶﺷ

:ﺪﻧﺪﺷ ﻢﯿﺴﻘﺗ ﯽﯾﺎﺗ 1

. ﺘﻔﻫ لﺮﺘﻨﮐ ﮥ

،لوا 2.

لﺮﺘﻨﮐ

ﺘﻔﻫ ﮥ ،ﺮﺧآ 3.

يرﺎﯿﺘﺧا ﻦﯾﺮﻤﺗ ،(راود خﺮﭼ) VT

4.

ﺎﻨﺷ ﻦﯾﺮﻤﺗ) يرﺎﺒﺟا ﻦﯾﺮﻤﺗ هوﺮﮔ (،ST

5 . (يژﻮﻟﻮﯾﺰﯿﻓ مﺮﺳ ،لﺎﯾور لژ لﻼﺣ) ﻢﺷ

، 6 .

لﺎﯾور لژ فﺮﺼﻣ ﺎﺑ هاﺮﻤﻫ يرﺎﯿﺘﺧا ﻦﯾﺮﻤﺗ )

VT+ RJ

( ، 7.

لﺎﯾور لژ فﺮﺼﻣ ﺎﺑ هاﺮﻤﻫ يرﺎﺒﺟا ﻦﯾﺮﻤﺗ )

ST+ RJ

و ( 8.

.لﺎﯾور لژ فﺮﺼﻣ

نﺎﯾﺎﺷ ﺖﺳا ﺮﮐذ ﻪﺑ

رﻮﻈﻨﻣ ﯽﺳرﺮﺑ تاﺮﯿﺛﺄﺗ ﺎﻘﻟا ي ﺮﺑAD

ﻐﺘﻣ ﯿ ﺎﻫﺮ ي ﻖﯿﻘﺤﺗ ﻪﺑ ناﻮﻨﻋ هوﺮﮔ يﺎﻫ لﺮﺘﻨﮐ 12 ﯽﯾاﺮﺤﺻ شﻮﻣ ﺮﺳ ﻪﺑ

هوﺮﮔ يﺎﻫ

ﺘﻔﻫ ﻢﻟﺎﺳ لﺮﺘﻨﮐ ﮥ

ﺘﻔﻫ ﻢﻟﺎﺳ لﺮﺘﻨﮐ و لوا ﮥ

ﻦﯿﻨﭽﻤﻫ .ﺪﻧﺪﺷ ﻢﯿﺴﻘﺗ ﺮﺧآ تر ﻦﺳ نﻮﭼ

ﺎﻫ ﺲﭘ اﺪﺣ ﻦﯾﺮﻤﺗ ﻞﮑﺗوﺮﭘ زا ﻪﺑ ﺮﺜﮐ

18 ﻪﺘﻔﻫ ﻣ ﯽ ﺳر ﯿﺪ

،

در ﺲﭘ ة دوﺪﺤﻣ رد ﺎﻬﻧآ ﯽﻨﺳ ة

شﻮﻣ ﺖﻓﺮﮔ راﺮﻗ ﻎﻟﺎﺑ ناﻮﺟ يﺎﻫ )

20 (.

و يرﺎﯿﺘﺧا ﯽﺘﻣﺎﻘﺘﺳا ﻦﯾﺮﻤﺗ ﻞﮑﺗوﺮﭘ ﺎﻨﺷ يرﺎﺒﺟا

تﺪﻣ ﻪﺑ ﻪﺴﻠﺟ ﺮﻫ و ﻪﺘﻔﻫ رد ﻪﺴﻠﺟ ﻪﺳ ،ﻪﺘﻔﻫ ﺖﺸﻫ تﺪﻣ ﻪﺑ يرﺎﯿﺘﺧا تﺎﻨﯾﺮﻤﺗ هوﺮﮔ 120

راود خﺮﭼ رد ﻪﻘﯿﻗد ﺑرد

ﻪﺘﺴ ﺖﻓﺎﺴﻣ و ﺪﻨﺘﻓﺮﮔ راﺮﻗ

ﯽﻃ زور ﺮﻫ رد هﺪﺷ ﺐﺴﺣﺮﺑ

ﺖﺒﺛ هﺎﮕﺘﺳد ﻂﺳﻮﺗ ﻪﻘﯿﻗد ﺮﺑ ﺮﺘﻣ ﺪﺷ

) 1(

. اﺰﯿﻣ و دﻮﺑ ﺮﮕﺷرﺎﻤﺷ ﻪﺑ ﺰﻬﺠﻣ نادﺮﮔ خﺮﭼ ﯽﻃ ﺖﻓﺎﺴﻣ ن

ﺪﺷ ة تر ﺮﻫ

ﯽﻣ ﺖﺒﺛ ار ﯽﻃ ﺖﻓﺎﺴﻣ ناﺰﯿﻣ .دﺮﮐ

ﺪﺷ ة ﺗ يﺎﻫزور مﺎﻤﺗ رد ،رﺮﻘﻣ ﺖﻋﺎﺳ سأر تر ﺮﻫ شرﺎﻤﺷ راود خﺮﭼ ﺮﺘﻧﺎﮐ ار ﺖﻓﺎﺴﻣ ﻦﯾا ؛ﺪﺷ ﺖﺒﺛ ﻖﯿﻘﺤ

رود ﺮﺧآ رد .دﻮﺑ هدﺮﮐ ة

هرﺎﻣآ ﺮﯾﺎﺳ و دراﺪﻧﺎﺘﺳا فاﺮﺤﻧا ﻦﯿﮕﻧﺎﯿﻣ ﯽﻨﯾﺮﻤﺗ يﺎﻫ

ﺮﻈﻧدرﻮﻣ ﻤﺗ يﺎﻫزور ﻞﮐ داﺪﻌﺗ ﻪﺑ ﺖﯾﺎﻨﻋ ﺎﺑ .ﺪﺷ ﻪﺒﺳﺎﺤﻣ ﯽﻨﯾﺮ

خﺮﭼ ﺎﻫ تر داﺪﻌﺗ ﺎﺑ ﺎﻬﻧآ ﯽﻧاﻮﺨﻤﻫ و راود ي ﺢﺒﺻ نﺎﻣﺰﻤﻫ ﻦﯾﺮﻤﺗ ﻞﮑﺗوﺮﭘ ،ﺎﻫ

ﺖﻋﺎﺳ ﺎﻫ 8

ﺎﺗ 10 اﺮﺟا ﻪﺑ ﻣرد ﯽ ﺪﻣآ نﺎﺷرﺎﯿﺘﺧا ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﻪﮐ

مﺎﺠﻧا ار ﺖﯿﻟﺎﻌﻓ ﻣ

ﯽ ﺪﻧداد تر ﺪﺷ ﺺﺨﺸﻣ ﻖﯿﻘﺤﺗ نﺎﯾﺎﭘ رد ، ﻦﯿﮕﻧﺎﯿﻣ ﺎﺑ ﺎﻫ

1000 ار يرﺎﯿﺘﺧا ﺖﯿﻟﺎﻌﻓ ،ﺮﺘﻣ راود خﺮﭼ يور

ﻪﺘﺴﺑرد ﺪﻧداد مﺎﺠﻧا

) 21 (.

شﻮﻣ رد ﯽﯾاﺮﺤﺻ يﺎﻫ هوﺮﮔ

يﺎﻫ ار ﺎﻨﺷ تﺎﻨﯾﺮﻤﺗ يرﺎﺒﺟا ﻪﺑ

ترﻮﺻ ﭘﯿ ﺶ هﺪﻧور رد ﻪﺘﻔﻫ رد ﻪﺴﻠﺟ ﻪﺳ ،ﻪﺘﻔﻫ ﺖﺸﻫ تﺪﻣ ﻪﺑ ، يﺎﻨﺷ ناو

تر نداد راﺮﻗ ﺎﺑ و ﺪﻧداد مﺎﺠﻧا نﺎﮔﺪﻧﻮﺟ صﻮﺼﺨﻣ ﺖﯿﻟﺎﻌﻓ ﻪﺑ راداو نﺪﺸﻧ ﻪﻔﺧ زا يﺮﯿﮔﻮﻠﺟ ياﺮﺑ ﺎﻨﺷ ناو رد ﺎﻫ

ﻣ ﯽ ﺪﻧﺪﺷ . ياﺮﺑ تﺎﻨﯾﺮﻤﺗ مﺎﺠﻧا

1 . Muscle Tremors

(7)

93

ﺘﻔﻫ رد ﺎﻨﺷ ﮥ

شﻮﻣ لوا تﺪﻣ ﻪﺑ ﯽﯾاﺮﺤﺻ يﺎﻫ

ﺞﻨﭘ ﺘﺧادﺮﭘ ﻦﯾﺮﻤﺗ ﻪﺑ ﻪﻘﯿﻗد ﺪﻨ

ﺘﻔﻫ و ﮥ ﻪﺑ نﺎﻣز تﺪﻣ ﻦﯾا ﺮﺧآ 60

.ﺪﯿﺳر ﻪﻘﯿﻗد نﺎﯾﺎﺷ

ﺳا ﺮﮐذ ﺖ

ﻦﯾﺮﻤﺗ تﺪﺷ ﺮﺑ ﻖﯿﻘﺤﺗ ﻦﯾا رد ﻪﮐ ﮐﺄﺗ

ﯿﺪ هﺪﺸﻧ

،

،كﻼﻣ ﻪﮑﻠﺑ ﻞﺻا زا هدﺎﻔﺘﺳا ﺎﺑ ﻪﮐ ﺖﻓﺮﮔ راﺮﻗ ﻦﯾﺮﻤﺗ نﺎﻣز تﺪﻣ سﺎﺳاﺮﺑ ﻦﯾﺮﻤﺗ ﻢﺠﺣ

ﻪﻓﺎﺿا رﺎﺑ ﺘﻔﻫ ﻪﺑ ﺖﺒﺴﻧ ﻪﺘﻔﻫ ﺮﻫ رد ﻦﯾﺮﻤﺗ نﺎﻣز تﺪﻣ ﮥ

ﻞﺒﻗ 10 ﺪﺷ ﻪﻓﺎﺿا ﻪﻘﯿﻗد )

22 ( .

لوﺪﺟ 1 . ﻣﺎﻧﺮﺑ ﮥ تﺎﻨﯾﺮﻤﺗ ﻧدﻮﻣزآ ﯽ ﺎﻫ ي هوﺮﮔ ﺎﻨﺷ ناو رد يرﺎﺒﺟا ﯽﺘﻣﺎﻘﺘﺳا تﺎﻨﯾﺮﻤﺗ

ﻢﺘﺸﻫ ﻢﺘﻔﻫ ﻢﺸﺷ ﻢﺠﻨﭘ مرﺎﻬﭼ مﻮﺳ مود لوا ﻪﺘﻔﻫ

60 60 60 45 35 25 15 5 (ﻪﻘﯿﻗد)ﻦﯾﺮﻤﺗ نﺎﻣز

لوﺪﺟ 2 . ﻮﺤﻧ ة ﺎﻨﺷ يرﺎﺒﺟا و يرﺎﯿﺘﺧا ﻦﯾﺮﻤﺗ عﻮﻧ ود ياﺮﺟا

ﺖﯿﻟﺎﻌﻓ ﻞﮐ نﺎﻣز تﺪﻣ ﺖﯿﻟﺎﻌﻓ ﻪﺑ ندﺮﮐ راداو شور لﺮﺘﻨﮐة ﻮﺤﻧ ﺖﯿﻟﺎﻌﻓ ﻢﺠﺣ ﻦﯾﺮﻤﺗ عﻮﻧ 2

ﺖﻋﺎﺳ

(دﻮﺑ يرﺎﯿﺘﺧا ﺖﯿﻟﺎﻌﻓ ﺪﯿﻔﻣ نﺎﻣز)

ﻈﻔﺤﻣ رد نداد راﺮﻗ ﮥ

خﺮﭼ ﻪﺘﺴﺑ نادﺮﮔ

ﺖﯿﻟﺎﻌﻓ نﺎﻣز تﺪﻣ سﺎﺳاﺮﺑ ﺖﯿﻟﺎﻌﻓ

يرﺎﯿﺘﺧا (نادﺮﮔ خﺮﭼ رد ﺖﻣﺎﻘﺘﺳا ود) 60

ﻪﻘﯿﻗد يرﺎﺒﺟا ﺖﯿﻟﺎﻌﻓ

و صﻮﺼﺨﻣ يﺎﻨﺷ ناو رد نداد راﺮﻗ ﺎﻨﺷ مﺎﺠﻧا ﻪﺑ رﻮﻈﻨﻣ ﻪﻔﺧ زا يﺮﯿﮔﻮﻠﺟ

نﺪﺷ

ﺖﯿﻟﺎﻌﻓ نﺎﻣز تﺪﻣ سﺎﺳاﺮﺑ

ﺖﯿﻟﺎﻌﻓ يرﺎﺒﺟا

(صﻮﺼﺨﻣ ناو رد ﺎﻨﺷ)

لﺎﯾور لژ فﺮﺼﻣ و ﻪﯿﻬﺗ هوﺮﮔ يﺎﻫ ﻪﻧازور ﻪﺘﻔﻫ ﺖﺸﻫ تﺪﻣ ﻪﺑ لﺎﯾور لژ فﺮﺼﻣ

1

mg.kg-

100 نآ زا ﻪﺑ ار ترﻮﺻ ﺖﻓﺎﯾرد ﯽﻗﺎﻔﺻ ﺮﮐ

.ﺪﻧد ﻪﺑ رﻮﻈﻨﻣ ﯿﻬﺗ ﮥ رﺎﺼﻋ ة لژ

لﺎﯾور 10 رد لﺎﯾور لژ مﺮﮔ 1000

ﯽﺳ ﻪﺘﺨﯾر ﺰﯾﺎﻧﻮﯾد ﺮﻄﻘﻣ بآ ﯽﺳ هﺪﺷ

تﺪﻣ ﻪﺑ طﻮﻠﺨﻣ و 16

يﺎﻣد رد ﺖﻋﺎﺳ 50

ﺟرد ﮥ ﯽﺘﻧﺎﺳ ﻪﺑﻮﮑﻧا داﺮﮔ

،ﺪﺷ يﺎﻣد رد و ﺪﺷ هداد رﻮﺒﻋ ﯽﻓﺎﺻ زا لﻮﻠﺤﻣ ﺲﭙﺳ 4

ﺟرد ﮥ ﯽﺘﻧﺎﺳ يراﺪﻬﮕﻧ داﺮﮔ ﺷ

زود ﺎﺑ ﻖﯾرﺰﺗ مﺎﮕﻨﻫ و ﺪ

1

mg.kg-

100 ﻪﺑ ترﻮﺻ

ﺪﺷ ﻖﯾرﺰﺗ ﯽﻗﺎﻔﺻ )

23 (.

ﻦﯾا رد ﻖﯿﻘﺤﺗ دﺎﻔﺘﺳا ياﺮﺑ ة

،لﺎﯾور لژ زا ﻪﻧازور 250

ﻣ ﯿﻠ ﯽ مﺮﮔ يﺎﻣد زا ﻪﻠﺻﺎﻓﻼﺑ لﺎﯾور لژ 21

- ﺟرد ﮥ ﺘﻧﺎﺳ ﯽ داﺮﮔ ﯽﺣاﺮﺟ يﻮﻗﺎﭼ ﺎﺑ

رد و هﺪﺷ جرﺎﺧ 3

ﻣﯿ ﻠ ﯽ ﻟﯿ ﺮﺘ ﻪﺑ ،ﺮﮑﯿﺷ ﻂﺳﻮﺗ ﺎﻬﻧآ لﻼﺤﻧا زا نﺎﻨﯿﻤﻃا زا ﺲﭘ ﻪﻠﺻﺎﻓﻼﺑ .ﺪﺷ ﻞﺣ ﻦﯿﻟﺎﺳ لﺎﻣﺮﻧ هوﺮﮔ

يﺎﻫ وRJ ST + RJ

تر نزو فﻼﺘﺧا ﻦﺘﻓﺮﮔ ﺮﻈﻧ رد ﺎﺑ و ﻢﯿﺴﻘﺗ شﻮﻣ ﺮﻫ ﻪﺑ ﺎﻫ

1

mg.kg-

100 ﻣ ﯿﻠ ﯽ مﺮﮔ ﺪﺷ ﻖﯾرﺰﺗ لﺎﯾور لژ لﻮﻠﺤﻣ نﺪﺑ نزو مﺮﮔﻮﻠﯿﮐ رد

,

)4

,

12 24 (.

48 ﺴﻠﺟ ﻦﯾﺮﺧآ زا ﺲﭘ ﺖﻋﺎﺳ ﮥ

،ﻦﯾﺮﻤﺗ شﻮﻣ ﺎﻫ ﺎﺑ 10 ) ﻦﯿﻣﺎﺘﮐ ﺪﺻرد 50

ﻣ ﯿﻠ ﯽ مﺮﮔ و (نﺪﺑ نزو مﺮﮔﻮﻠﯿﮐ رد 2

) ﻦﯾزﻼﯾز ﺪﺻرد 10

ﻣﯿ ﻠ ﯽ مﺮﮔ (نﺪﺑ نزو مﺮﮔﻮﻠﯿﮐ رد ﺑ

ﯽ شﻮﻫ مﺎﻤﺗ ﺲﭙﺳ .ﺪﻧﺪﺷ ﺖﻤﺴﻗ

ﺎﻫ ي ﺖﻓﺎﺑ ﺎﻫ ي جاﺮﺨﺘﺳا نﺎﺼﺼﺨﺘﻣ ﻂﺳﻮﺗ ﭗﻣﺎﮐﻮﭙﯿﻫ ﺪﺷ

بﻮﺗﻮﯾﺮﮐ رد و

رد يﺎﻣد رد ﺮﺘﺸﯿﺑ ﯽﺳرﺮﺑ ياﺮﺑ و ﺪﻨﺘﻓﺮﮔ راﺮﻗ ﻊﯾﺎﻣ نژوﺮﺘﯿﻧ 70

- ﺟرد ﮥ ﺘﻧﺎﺳ ﯽ داﺮﮔ نژ نﺎﯿﺑ .ﺪﻧﺪﺷ هﺮﯿﺧذ

NF-κB

، شور ﺎﺑB2M Real

Time PCR

هزاﺪﻧا ﮔﯿ ﺮ ي .ﺪﺷ

(8)

94

شور ﺎﺑ نژ نﺎﯿﺑ RT-qPCR

ياﺮﺑ هزاﺪﻧا ﮔﯿ ﺮ ي نﺎﯿﺑ ناﺰﯿﻣ

NF-κB

و زا ﺲﭘ وB2M

ﻦﮕﻤﻫ زﺎﺳ ي ،ﭗﻣﺎﮐﻮﭙﯿﻫ ﺖﻓﺎﺑ ﺗوﺮﭘ ﻖﺒﻃRNA

) ﺪﺷ جاﺮﺨﺘﺳا ﺖﮐﺮﺷ ﻞﮑ

Qiagen,

Germany

رد رﻮﻧ بﺬﺟ يﺮﺘﻣﻮﺘﻓوﺮﺘﮑﭙﺳا شور .(

260 صﻮﻠﺧ ﯽﺑﺎﯾزرا ياﺮﺑ ﺮﺘﻣﻮﻧﺎﻧ ﺨﺘﺳا زا ﺲﭘ .ﺪﺷ هدﺎﻔﺘﺳاRNA

جاﺮ صﻮﻠﺧ ﺎﺑRNA

،بﻮﻠﻄﻣ ﺖﻈﻠﻏ و ) ﺪﺷ ﺰﺘﻨﺳ هﺪﻧزﺎﺳ ﻞﮑﺗوﺮﭘ ﻖﺒﻃ cDNA

Fermentas, USA

ﺲﭙﺳ و ( مﺎﺠﻧا ياﺮﺑ هﺪﺷﺰﺘﻨﺳ cDNA

ﺲﮑﻋ ﻞﻤﻌﻟا

ﯾﻮﻧور اﺪﺘﺑا .ﺪﺷ هدﺎﻔﺘﺳا سﻮﮑﻌﻣ ﯽﺴ ﯽﻣﺎﻤﺗ

يﺎﻫﺮﮔزﺎﻏآ ﺣاﺮﻃ

ﯽ ﺪﺷ ة ﻤﻫ ﻪﺑ طﻮﺑﺮﻣ ﮥ

نژ ﺎﻫ ﯽﺳرﺮﺑ ﺪﻧﺪﺷ ﺲﭙﺳ و ﺑ نﺎﯿ نژ ﺎﻫ شور زا هدﺎﻔﺘﺳا ﺎﺑ

توﺎﻔﺗ سﺎﺳاﺮﺑ ﯽﺒﺴﻧ ﯽﻤﮐ وCt

q-RT PCR

ﺪﺷ ﯽﺳرﺮﺑ .

ﯽﻤﮐ ﺲﭙﺳ لﻮﻣﺮﻓ زا هدﺎﻔﺘﺳا ﺎﺑ ﺎﻫﺮﯿﻐﺘﻣ ﺢﻄﺳ يزﺎﺳ

2 - ﻧاΔΔCT

مﺎﺠ نﺎﯾﺎﺷ .ﺖﻓﺮﮔ ﯽﺒﺴﻧ نﺎﯿﺑ ﻪﮐ ﺖﺳا ﺮﮐذ

نژ ﺎﻫ ﮏﯾ زا هدﺎﻔﺘﺳا ﺎﺑ

ﯽﻠﺧاد لﺮﺘﻨﮐ نژ هدﺎﻔﺘﺳا درﻮﻣ يﺎﻫﺮﮔزﺎﻏآ .ﺪﺷ ﻪﺴﯾﺎﻘﻣ ﻢﻟﺎﺳ لﺮﺘﻨﮐ هوﺮﮔ نژ ﺎﺑ نآ نﺎﯿﺑ ﺲﭙﺳ و ﻪﺴﯾﺎﻘﻣ B2m

ﺖﯾﺎﺳ زا هدﺎﻔﺘﺳا ﺎﺑ ﺰﯿﻧ

PUBMED

زا هدﺎﻔﺘﺳا ﺎﺑ ﺮﮔزﺎﻏآ ﯽﯾارﺎﮐ و هﺪﺷ ﯽﺣاﺮﻃ مﺮﻧ

راﺰﻓا .ﺪﺷ ﯽﺑﺎﯾزرا ﺖﯾﺎﺳ رد دﻮﺟﻮﻣ

لوﺪﺟ 1 . سﻮﮑﻌﻣ و ﻮﻠﺟ ﻪﺑ ور يﺎﻫﺮﻤﯾاﺮﭘ ﯽﻟاﻮﺗ نژ

ﺎﻫ ي Nf-KB و ﺶﻨﮐاو ياﺮﺑB2m Real-time PCR

Gene Forward (5’-3’) Reverse (5’-3’) Product

Size, bp

B2m 5’-CGTGCTTGCCATTCAGAAA-3’ 5’-ATATACATCGGTCTCGGTGG-3’ 244

NF-κB 5’-CAGGAAGAATGTCGCGCCTT-3’ 5’-AAGCCATTCTCCTGATGTCCG-3’ 134

MDA 5’-CATTGAGAATGTCGCGTCGG-3’ 5’-CCGCAATTCTCCTGATGTCCG-3’ 251

TAC 5’-CGTTGAGAATGTCGCGTCAA-3’ 5’-TTGCCATTCTCCTGATGTCCG-3’ 132

شور هزاﺪﻧا ﮔﯿ ﺮ ي يد نﻮﻟﺎﻣ ﺪﯿﺋﺪﻟآ

يد نﻮﻟﺎﻣ ﺪﯿﺋﺪﻟآ

) ( MDA

) ﺖﯿﮐ زا هدﺎﻔﺘﺳا ﺎﺑ

ZellBio,Cat.No.ZB-MDA-A96A.Gmbh. Germany

( نﺎﻤﻟآ ﺖﺧﺎﺳ ﯽﺳرﺮﺑ

ﺪﺷ

.

ياﺮﺑ هزاﺪﻧا ﮔﯿ ﺮ ي ﻪﺑMDA

ناﻮﻨﻋ ﺖﺴﯾوا و گﻮﯿﺑ شور زا ﯽﺑﺮﭼ نﻮﯿﺳاﺪﯿﺴﮐاﺮﭘ ﺺﺧﺎﺷ لﻮﮑﻟﻮﻣ ﻦﯿﺑ ﺶﻨﮐاو زا شور ﻦﯾا رد .ﺪﺷ هدﺎﻔﺘﺳا1

تﺎﺒﯿﮐﺮﺗ ﻪﮐ ﺪﯿﺳا ﮏﯾرﻮﺘﯾرﺎﺑﻮﯿﺗ لﻮﮑﻟﻮﻣ ﺎﺑMDA

ﮓﻧرﺰﻣﺮﻗ دﺎﺠﯾا

ﻣ ﯽ ﺪﻨﮐ

، هدﺎﻔﺘﺳا ﻣ ﯽ دﻮﺷ ﮏﯿﺘﺳاوﺮﻠﮐ يﺮﺗ زا ﯽﻟﻮﻠﺤﻣ اﺪﺘﺑا ﺐﯿﺗﺮﺗ ﻦﯾا ﻪﺑ . ،2

ﮏﯾﺮﻠﮐ ﺪﯿﺳا و ﺪﯿﺳا ﮏﯾرﻮﺘﯿﺑرﺎﺑﻮﯿﺗ تﺪﻣ ﻪﺑ و هﺪﺷ طﻮﻠﺨﻣ يرﺎﮐ ﻪﻧﻮﻤﻧ ﻪﺑ ﺺﺨﺸﻣ ﺖﺒﺴﻧ ﺎﺑ و ﻪﯿﻬﺗ3

10 ﺎﺗ 15 هداد راﺮﻗ يرﺎﻣ ﻦﺑ رد ﻪﻘﯿﻗد

ﻣ ﯽ ﺪﺷ تﺪﻣ زا ﺪﻌﺑ . ﻌﺗ

ﯿﯿ ﻦ هﺪﺷ ﻦﺑ زا طﻮﻠﺨﻣ و جرﺎﺧ يرﺎﻣ

ﻪﻠﺻﺎﻓﻼﺑ ﺪﺷ دﺮﺳ بآ زا هدﺎﻔﺘﺳا ﺎﺑ

رود رد و ه 2500

ﻔﯾﺮﺘﻧﺎﺳ ﯿ ژﻮ ﻣ ﯽ ﻮﺷ د . ﺲﭘ زا

ﻔﯾﺮﺘﻧﺎﺳ ﯿ ژﻮ ﻪﺑ ﻣارآ ﯽ ﯾﻻ ﮥ ﺖﯿﺳوﺮﺘﯾرا زا ﯽﯾور ﺮﺘﻣﻮﻧﺎﻧ جﻮﻣ لﻮﻃ رد يﺮﺘﻣﻮﺗﻮﻓﻮﺘﮑﭙﺳا هﺎﮕﺘﺳد زا هدﺎﻔﺘﺳا ﺎﺑ و هﺪﺷ اﺪﺟ ﺎﻫ

532 بﺬﺟ تﺪﺷ

هﺪﻧاﻮﺧ يرﻮﻧ ﻣ

ﯽ ﺪﺷ ﺞﯾﺎﺘﻧ . ﻪﺑ ترﻮﺻ .ﺪﺷ نﺎﯿﺑ ﻦﯿﺑﻮﻠﮔﻮﻤﻫ مﺮﮔ رد لﻮﻣﻮﻧﺎﻧ

1 . Buege & Aust

2 . Trichloroacetic Acid

3 . chloric Acid

(9)

95

شور هزاﺪﻧا ﮔﯿ ﺮ ي ﺖﯿﻓﺮﻇ ﺘﻧآ ﯽ ﺴﮐا ﯿ ناﺪ ﺎﻤﺳﻼﭘ مﺎﺗ

ﺖﯿﻓﺮﻇ ﺘﻧآ ﯽ ﺴﮐا ﯿ ﺖﻧاﺪ ) ﺎﻤﺳﻼﭘ مﺎﺗ ( TAC

) ﺖﯿﮐ زا هدﺎﻔﺘﺳا ﺎﺑ

ZellBio,Cat.No.ZB-TAC-A96.Gmbh. Germany

( نﺎﻤﻟآ ﺖﺧﺎﺳ

ﺪﺷ ﯽﺳرﺮﺑ شور ﻦﯾا رد .

3-ethylbenzthiazoline suphonate

) زاﺪﯿﺴﮐاﺮﭘ ﺎﺑ

metmyoglobin

ﺪﯿﻟﻮﺗ ﺖﻬﺟ نژورﺪﯿﻫ ﺪﯿﺴﮐاﺮﭘ و (

نﻮﯿﺗﺎﮐ لﺎﮑﯾدار

3-ethylbenzthiazoline suphonate

نﻮﯿﺳﺎﺑﻮﮑﻧا ﻣ

ﯽ دﻮﺷ ﯽﺑآ ﮓﻧر ﺪﯿﻟﻮﺗ ﻞﺻﺎﺣ ﮥﺠﯿﺘﻧ . -

ﻪﮐ ﺖﺳا يﺪﻨﻧﺎﻣ ﺰﺒﺳ

ًﺎﺘﺒﺴﻧ

جﻮﻣ لﻮﻃ رد و راﺪﯾﺎﭘ 600

ﻞﺑﺎﻗ لﻮﻣﻮﻧﺎﻧ هزاﺪﻧا

ﮔﯿ ﺮ ي ﺖﺳا . ﺘﻧآ ﯽ ﺴﮐا ﯿ ناﺪ ﯽﻧﻮﺧ ﮥﻧﻮﻤﻧ رد دﻮﺟﻮﻣ ﻟﻮﺗ زا

ﯿﺪ و ﺖﻈﻠﻏ ناﺰﯿﻣ ﻪﺑ ﻪﺟﻮﺗ ﺎﺑ ﮓﻧر

يﺮﯿﮔﻮﻠﺟ دﻮﺧ ترﺪﻗ ﻣ

ﯽ ﺪﻨﮐ .

شور يرﺎﻣآ

ﻪﺑ رﻮﻈﻨﻣ ﻪﯾﺰﺠﺗ تﺎﻋﻼﻃا ﻞﯿﻠﺤﺗو ﺮﻈﻧدرﻮﻣ

ﻪﻘﺒﻃ ،ﻒﯿﺻﻮﺗ ياﺮﺑ ﯽﻔﯿﺻﻮﺗ رﺎﻣآ زا .ﺪﺷ هدﺎﻔﺘﺳا ﯽﻃﺎﺒﻨﺘﺳا و ﯽﻔﯿﺻﻮﺗ رﺎﻣآ زا تاﺮﻤﻧ ﻢﯿﻈﻨﺗ و يﺪﻨﺑ

ﺮﯾدﺎﻘﻣ ﻦﯿﻨﭽﻤﻫ .ﺪﺷ هدﺎﻔﺘﺳا رادﻮﻤﻧ و لوﺪﺟ ﻢﺳر ،دراﺪﻧﺎﺘﺳا فاﺮﺤﻧا ،ﻦﯿﮕﻧﺎﯿﻣ ﮥﺒﺳﺎﺤﻣ ﻖﯾﺮﻃ زا مﺎﺧ ﻐﺘﻣ

ﯿ ﺎﻫﺮ درﻮﻣ ي هزاﺪﻧا ﮔﯿ ﺮ ﺳﻮﺗ ي ﻂ

ﺺﺧﺎﺷ ﯽﻃﺎﺒﻨﺘﺳا رﺎﻣآ رد .ﺪﺷ ﻒﯿﺻﻮﺗ ﯽﮔﺪﻨﮐاﺮﭘ و يﺰﮐﺮﻣ يﺎﻫ ﯽﻌﯿﺒﻃ ﻊﯾزﻮﺗ ﯽﺳرﺮﺑ ياﺮﺑ

هداد ﺎﻫ وﺮﯿﭘﺎﺷ نﻮﻣزآ زا -

ياﺮﺑ و ﮏﻠﯾو

ﻪﯾﺰﺠﺗ ﻞﯿﻠﺤﺗو هداد ﺎﻫ ) ﺲﻧﺎﯾراو ﻞﯿﻠﺤﺗ نﻮﻣزآ زا

ANOVA

ﻪﺑ ﻦﯿﻨﭽﻤﻫ .ﺪﺷ هدﺎﻔﺘﺳا ﻪﻫاﺮﮑﯾ ( ﮥﺴﯾﺎﻘﻣ رﻮﻈﻨﻣ

ﻪﺑود ود ي هوﺮﮔ ﺎﻫ نﻮﻣزآ زا

t

ﻪﯾﺰﺠﺗ .ﺪﺷ هدﺎﻔﺘﺳا ﻞﻘﺘﺴﻣ زا هدﺎﻔﺘﺳا ﺎﺑ ﻞﯿﻠﺤﺗو

مﺮﻧ راﺰﻓا

SPSS 22

يرادﺎﻨﻌﻣ ﺢﻄﺳ رد 05

/0

α=

.ﺖﻓﺮﮔ مﺎﺠﻧا

ﻪﺘﻓﺎﯾ ﺶﻫوﮋﭘ يﺎﻫ

حﻮﻄﺳ ،TAC

، B2M

وMDA NF-κB

ﭗﻣﺎﮐﻮﭙﯿﻫ ﺖﻓﺎﺑ رد شﻮﻣ

ﺎﻫ ﻪﺑ يﺎﻫرادﻮﻤﻧ رد ﺐﯿﺗﺮﺗ 1

ﺎﺗ 4 .ﺖﺳا هﺪﺷ شراﺰﮔ

ﻞﮑﺷ 1 ﺮﯿﻐﺘﻣ رﺎﯿﻌﻣ فاﺮﺤﻧا و ﻦﯿﮕﻧﺎﯿﻣ . NF-κB

رد هوﺮﮔ يﺎﻫ ﺮﯿﻐﺘﻣ ياﺮﺑ ﯽﻧﺎﻣرد NF-κB

+ (ﺎﻨﺷ) يرﺎﺒﺟا شزرو هوﺮﮔ رد ﻦﯿﮕﻧﺎﯿﻣ ﻦﯾﺮﺘﺸﯿﺑ * .

لﺎﯾور لژ فﺮﺼﻣ 113

/ 1 29447± ﺘﻔﻫ ﻦﯿﮕﻧﺎﯿﻣ ﻦﯾﺮﺘﻤﮐ** .دﻮﺑ ﮥ

هوﺮﮔ رد ﺮﺧآ 434AD

/ 0 429± / 25 .دﻮﺑ

*

**

0 5 10 15 20 25 30 35

NFKB(MEAN)

ﺎھ هوﺮﮔ

(10)

96

ﻞﮑﺷ 2 ﺮﯿﻐﺘﻣ رﺎﯿﻌﻣ فاﺮﺤﻧا و ﻦﯿﮕﻧﺎﯿﻣ . ﺮﯿﻐﺘﻣ ياﺮﺑ ﯽﻧﺎﻣرد يﺎﻫ هوﺮﮔ ردB2M

(ﺎﻨﺷ ) يرﺎﺒﺟا ﻦﯾﺮﻤﺗ هوﺮﮔ رد ﻦﯿﮕﻧﺎﯿﻣ ﻦﯾﺮﺘﺸ ﯿﺑ * .B2M 092

/ 6

±

012 / 3 1 (ﺎﻤﻧوراد) ﻢﺷ هوﺮﮔ رد ﻦﯿﮕﻧﺎﯿﻣ ﻦﯾﺮﺘﻤﮐ ** .دﻮﺑ 568

/ 2 0 41 ± / 25 .دﻮﺑ

ﻞﮑﺷ 3 ﺮﯿﻐﺘﻣ رﺎﯿﻌﻣ فاﺮﺤﻧا و ﻦﯿﮕﻧﺎﯿﻣ . هوﺮﮔ ردMDA

ﺮﯿﻐﺘﻣ ياﺮﺑ ﯽﻧﺎﻣرد يﺎﻫ هوﺮﮔ ﺮﺧآ ﮥﺘﻔﻫ رد ﻦﯿﮕﻧﺎﯿﻣ ﻦﯾﺮﺗﻻﺎﺑ * .MDA

6297AD 12771±

لوا ﮥﺘﻔﻫ ﻢﻟﺎﺳ هوﺮﮔ رد ﻦﯿﮕﻧﺎﯿﻣ ﻦﯾﺮﺘﻤﮐ** و دﻮﺑ 2024

5633± .دﻮﺑ