1.
!"#$%
&'( ! ")#*
+,- .# /0*12314$$%556%7 '8 9:&0;<=>?'@$(A)*8+
7( B! $,- C.- /DE01F 2G7HH+ IJK31L4&MN 5$ !0;<=>6A"'8&
'( 7 8OP9Saccharomyces cerevisiaeQJRS
;T:U;P9Yarrowia lipolytica17 !$V"' W%!(
2.
(1) !"#$%&
")#$Q7 *+,X2TYZ?*
!< !$J[W6\'( ]B^B*+,8_
`!#= <>&'*+,a?b$ ! ]\@c\de78 9JK !Af;
gB"'hC)&%!( D ")#=<?*
+,$9\e$Af&SNARE2TYZid\
'j2TYZ8E`Q[ < !Af;$5 56'I8FB]\7!( B! Gk#HISNARE 2TYZ17!AJ5e SNARE2TYZ !<
2lmn'*+,@c]o'I8p' I8Kq]\7!(
9JKLrH 7 8OP9HIMN SNARE2TYZOstuvwx>17 yX
<HI<z{ z{HI<#
3PQ#=< !@c|Rin vitro}
!( 9~ 9\e@c$556'v-SNAREt-
SNARESc8)e5[I\eSNARE2TY
Z8@c]o'hTHI8K]\!U1(I\
e~ 5ev-SNAREHIhelixt-SNARES cHIhelix89\eVW X
Y-"'I$J[Z8poe\
@c"'7K #=@c$V"'[;H7]\
!( ]e$ I\e3Pt-SNAREScP9="
v-SNARE[K'SNARE2TYZOstuv
wx>17@c *\]7
SNARE2TYZOsc6o8?7^c$@c8
I'$_ ?7^c$@c7I)e 5$ v-SNAREt-SNAREOsc6o$J[@c Af;8gB]\'IK!(
(2) '(
+`5e]\Q[
c ! A} vvT 8a$]
\'I$J[b;84$]\7'(IK
c9'@$Vc&AJ8du7'$
_ !?A?vvT$7(A)
*8+]\7'( I AB
eU"' ¡8f7I? g
<2"'I8¢h&'I8£¤e\'( 7
¥&AJi¦8OP917I\ejkl$m[
Os n&'vT !
$V"' W%!(
oC§$pQ[ Zq=
qXHr_s$E`7'( /1¨©ª{2«S
¬T(PE)")#nvT&[8OP9
$Q7$&'( ®5e]B^B P$Q7PE#=qt$u%p"'I8Kq ]\Q[ A$##$Q7#oPE#v
¯°8W6\p!8 PEr_sp-±4$$55 6'=qX !hC)&%!( 9I wxe PE$Af&$~c"'²Ro09-90198 (Ro)yz 8OP9QJR{P9Schizosaccharomyces pombe
#q$E`"'PE¯° #$Q7Vb=
qt$+7]\'PE8#|³AB}³$ ;*´
Xqa~8"'IK!U2(]e$¥g
µ¶17!AJ5e P·ATPase&'Dnf 1QJR Dnf2#2TYZ&'Ros38 #$Q7
qt$~8!PE=qa !$556'IKq
!( B! P9¥gµ¶¸Z3¹T5eRoº;¶
+Q[ I\eAJ$J[#oPEr_s p$556'2TYZ8)e5$'H³7
)1 v-SNAREt-SNAREScY
*
x»¼ ½U 29
!"# $%&'(
#PE')*+,-./01(PC)2 (#3456 7 )89 ( PE:PC& ;<=>
8?@A BC (#DEF
" &'(#PE:PCGC
HIJ7 ! 9 K
LM3"6 !N)8 !N@OPOQ56 3 6R!J"N6
3.
Y. lipolyticaS:#T$UR%&' V
W X(()9Y %&*+Z[,"6W;' \]KJ^-_N&OQ Y. lipolytica
*+Z"6./O.0 12TW
`/abcP450 (P450ALK)3dJ45_N ! )eRf6S _@g@RVW&&'(#9h 7ij_N#(6J )eRX89:RW
;')5#;kM<l=>fJ?m_NHIS@O Q56
AnS Y. lipolytica'(#.0oX83
d45_NP450ALK1pq ALK1>f?m2 (#34( rcpspBCDARE1t;
bHLHu3dv&WwYas 1Yas 2x(y"6
Yas1Yas2SE;z" ARE1t;"#ALK1 3dv&W !JR56{F3|_}~
Yas2t;"#Yas1ῌYas2)3dv&WGH wYas39IJ"6 !Nw$%&'
cS ;&+l=3d?m`K 3dwOQIno4')*Ino2,3dL?wOQOpi1 OQ! M&' ;BNO(
PJR56
S QRRS T K T
UM<l= O4`N69OQ8 V
W68XR5 Y8@"6QR
Z[\] ^)8B_"B@ n"#
`5(6y@"6QRῌaK!]
mb_"B@ VeOcRRY
d@"(6y@"6QR efgh] O
B_"B@ @6 5(6y@"6QiR
¡[gj] M<l= ¢]k^)8mb _"B@ S M<l= R cRRl)6@9OQ8 m£ 7¤ OB (6"@ nSo¥Memorial Sloan-Kettering Cancer CenterO4`N69OQ8 5 pq8
@"6fColumbiaRJames E. Rothman] R*
fHeidelbergRThomas H. So¨llner] mb_"
B@ @6 ¦I _A#(6y@"6f§]
B_"B@ }¨R8@"6J rds
tuy_(@"6vS©WªQ«nw¬x[¬®
] R*«¯8@"6¢] mb_"B
@ 2 &'M<§y]PEz$M<°^
M<B{(±pO²"6nS§y|PEJz
$"#(n³{(±p´Sk @6}(
±pqµaSb/¶µ/²
3 Yas1, Yas2, Yas3)·3d?m