O-
!"#$"% &'()
*+&,- ./012!3456!" ! /7 8#9$%&:'(4;!<=) !"% &'(
)>*+,-7 ?@ABCD.$/7 E CD>*&'()0*FGH,GI'J "% K /7 O-0*FGH,GI'&'()0J7 L1 M2N3O"E44#P!Q.% 567
8A. awamoriO"0@FRS1,T9:
U4VDP!Q.7 ;<!O-WXY9:X=
ZNO">[#\7 ?.]"O-WXY9:
X= ZU4V@A;V^B;.% C_7 0@
FRS1,T(GAI)O-WXY9: 4]"O- WXY9:X= Z`!Da;.%
1. O-
8EFbO" GAI 7 NGHcId'e 'fVgJUO"KLhij'(A1ῌV469),Z=45RSk MNflm30RSkMNfSerThr#"9 :WXhij' (Gp-I: A470ῌV514), ;!CGHd'e' nOhij'(A515ῌR615)#"%Gp-ISer/ThrPQ O-Y(o'k,p1q3Nf#"9:RWX;
!"% 56Gp-IPQSrV<`TsSrYGAV ZU;7 Vt8&:uSrYGAVWv .% Ww7 Gp-IV36RSkMNfxXyY;.GA Z4#/7 [,\!]tU "E4V[z;
.% Z#SrYGAV&:u.^7 &'() _{`|"+,-G}~'Bipabcde rfE"E47 Gp-I yY/GA >*
g/..;\D| !#E4h .% ;.P
!7 Gp-Iijhij'#" GAIkGHhij'V g/..u7 #GAI4;!V<.
Ol$"%.7Gp-IVyY;.SrYGAd 'e'U 40m;7Gp-IVn`&'d2
<`SrYGA!oU 4o0J .% .Vt8O-WXY9:X=Sr!
GAI9$%V&:u79:pq;.GAVu.% N-WXY9:4r#/O-WXY9:GAI N
#\7 04d'e'UN3O"E44
#P.%
2. O- !
O-WXY9:N-WXY9:4r#/7 /
rg "9s=tZtQ\r#"% uvO-WX Y9:wVx:<1q5Nf#"o'k,p :7Aspergillusy;<1q3Nf#"x:<
;.o'k,p:t7 pzm0@F,p7 1)
,p<Z=94;!E4> !"% 9&'(
)9:X= Z4 V4O".67 v
$"A. nidulansO-WXY9:X= ZU4V{
.% 7 A. nidulansO-WXY9:X=|&}(Ser/
ThrDol-P-MannoseSer/Thr-MannoseDol-P)VKLO
"protein:O-9-mannosyltransferase (Pmt)V),'J;7 3Tpmt9$%(pmtA,pmtB,pmtC)ZtV4;
.% pmtA,pmtB,pmtC9$%Zt~7 +,-L1
&'()$"40 .% A. nidulans! pmtA,pmtB,pmtCP!7 <0p&:
;!"E47 ;$"E4h .%
7 PMT"9:X=}\9aC}VKL O"a-o'kG@1'p1,Ta-1)G@
1'p1,TVF,hO"40 "s3Tmnt 9$%(mntA,mntB,mntC), 2Tgct9$%(gctA,gctB)V)
,'J;7 ZtV4;.% mntgct9$%
<IIY&'()ZtV=;7?@A-L 1O"<40 .% mntA,mntC=0p
&:O";7 mntB=pHp
&:;!/7`f#l h .%gctA gctB&:pm7 Z=f&:;!.%
3. pmtA"#$
A. nidulanspmtA9$%Vos¡¢/£;.
pmtA£VZU;7 o9$% UVDP.% pmtA
£7 in vitroPmt 4zv6¤ m;.% .7 pmtA£F¥,¦j§zv
40¤bm;7 %j<zv20¤*Rq
;.% .7 £;Fr#;Zt
7 ;¨©1O"ªJEP!.%
£[,WXO"«g#¬
#:SJ6 .% [,=VWv.W w7pmtA£7[,fCV#Ob-1,3/1,6-0@
®'pzvq;!/7 f©V 6"
l@V#Oa-0@®'pg;!.% O#|m7 £
[,¡J;!/7 pmtA#[,Zt=.
¢$"E44#P.% 7 n£¯¤°±¥U
"¦4pmtA£&'()e²j@Vz
³;.% k©!7 e[,7 [,1O"&
'()e²j@ [ .% PmtA&'(
)#7 o'kG@J}f$"[,´[,
&'() 7 07 $"L1#lV µ¶!"E4h "·§1¸% pmtA9$%£"
r#:Y¨©¹Eª/º«Q"E4t7
£[,F»¼'pgO"E47 PmtA
¬6_µ ½J®¯¬ % 23
cell wall in- tegrity mechanism
!"#A. awamori$%&pmtA ' () *' (+),- A. awamori pmtA' (.A. nidulans pmtA' (+ / 0123- *456(7 .89:;- A. awamori pmtA+ GAIO-<=1>.?@AB33
@ACPmtAGAIO-<=1> D%
E)F- G- GAIHI!"# pmtA+ GAI.$&%J/? GAI%.
PmtA O-1<=>..K& ./%E )'3L-
ῌ ῏ ῎ ῍
>.()8MN,*OP Q+ R STUV WXRYZ[ \,]/^-_)
`- E.3$9 *K&/a0%
L # >Vb'6cdecY.%Gf12 3!4gh .#O-<=1>=V, .i O-<=1> D 75)jk4l\, )%L6m%L)b'n- "#pmtA +. I!"# .OP$9
>10op6O-<=1>cdecY
op7q8
4gh.rs%t%tu9tghu:Vvt;<w=
:txy1zt$ j{-& !9G #)>
|-4gh)?j V})~%-fn a@
A)9G-rs%t%tu9tghu1zt$
BCDE/)G G-
A)9G-rs%t%tu9tghu1z t$$FGH IJKHD?%-G 4gh`.1zt$ tf%tu6t%
/LM: !9 N OL3P
G Q HR=gh STH %%t
U<H V%tW$XH Il KwonH.
%/ ¡)%-fn D?PG 4gh)¢
n£L¤%/a@)9G-rs%tYZ
¥¦ [\]§CDE/)G 4gh^
j A)9G-rs%tYZ WV%t
¨_©COL3PG G- ªK/
gh«¬)9G-c%tAnanda M. Chakrabarty CD?PG ®¯4°A±`?f
%G-rs%t%tu9tghu=:²t$ ³´
TCµ?maPG ῑ1 PmtA8O-R[8£6m#¶