講 演 要 旨 集 - 日本農芸化学会中四国支部

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᪝ᮇ㎨ⰹ໩Ꮥఌ୯ᄿᅗᨥ㒂㻃

➠䠃䠊ᅂㅦⁿఌ㻃

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ㅦ㻃 ⁿ㻃 さ 㻃 ᪠㻃 㞗 㻃

㻃 㻃 㻃 㻃 㻃 㻃

᪝᫤䠌㻕㻓㻓㻚 ᖳ䠇᭮ 㻔㻕 ᪝䟺ᅰ䟻㻃 㻔㻖 ᫤㛜ఌ㻃 ሔᡜ䠌┬❟ᗀᓞኬᏕᗀᓞ䜱䝧䝷䝕䜽㻃

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日本農芸化学会中四国支部

1 

᪝ᮇ㎨ⰹ໩Ꮥఌ୯ᄿᅗᨥ㒂➠䠃䠊ᅂㅦⁿఌ䟺ౚఌ䟻㻃

ུ㈳ㅦⁿ䝿≁ืㅦⁿ㻃 䟺㻔㻖䠌㻓㻓䡐㻔㻗䠌㻘㻘䚮㻕㻔㻗㻖 ኬㅦ⩇ᐄ䟻㻃

㻕㻓㻓㻚 ᖳᗐ㎨ⰹ໩Ꮥዜທ㈳ུ㈳ㅦⁿ䟺㻔㻖㻝㻓㻓䡐㻔㻖㻝㻖㻓䟻㻃

ࠔකྙᠺ⏍∸࡞࠽ࡄࡾ࢓ࢪࢤࣜࣄࣤ㓗࢛࣋ࣜ࢞ࢨࢱ࣭ࢭࡡⓆ⌟ㄢ⟿ᶭᵋ࡛⏍⌦ᶭ⬗ࡡ よ ᪺ࠕ ▴ᕖ Ꮟ༡㸝ᓞ᰷ኬᏕ⏍∸㈠″ ⛁Ꮥ㒂㸞

ᗑ㛏 Ờᆊ ᏺ᫋㸝ᗀᓞኬ㝌࣬⏍∸ᅥ⛁Ꮥ◂✪⛁㸞

㻕㻓㻓㻚 ᖳᗐᨥ㒂ዜທ㈳᤭୙ᘟ䟺㻔㻖㻝㻖㻓䡐㻔㻖㻝㻗㻓䟻㻃

㻕㻓㻓㻚 ᖳᗐᨥ㒂ዜທ㈳ུ㈳ㅦⁿ䟺㻔㻖㻝㻗㻓䡐㻔㻗㻝㻕㻘䟻㻃

㸦㸣 ࠔ⥲⹰ࡡఆ┸ ࣬ᑋ࿤โᚒᶭᵋ࡞㛭ࡌࡾ໩Ꮥ⏍∸ᏕⓏ◂✪ࠕ ἑ㔕 ᙁ㸝㫵ཱིኬᏕ㎨Ꮥ㒂㸞

ᗑ㛏 ᲻ ಘᐰ㸝㫵ཱིኬ㝌࣬㏻ྙ㎨Ꮥ◂✪⛁㸞

㸧㸣 ࠔ᳔∸⢶ࢰࣤࣂࢠ㈻⢶㙈ࡡ௥ㅨᶭᵋ࡛࢓ࣝࣜࢣࣤ⢶㙈ࡡᵋ㏸࣬ᶭ⬗ よᯊࠕ ๑⏛ ᜠ㸝ᕖᓧ༈⛁ኬᏕ⾠⏍Ꮥ㸞

ᗑ㛏 ⛼ᇄ ㈶஦㸝ᒱᒜኬ㝌࣬⮤↓⛁Ꮥ◂✪⛁㸞

㸨㸣 ࠔ'FHLOOXV ᒌ⣵Ⳟ⏜ᮮࡡ᩺ぞ ⎌≟஫⢶⏍ᠺ㓕⣪࡞㛭ࡌࡾ◂✪ࠕ

ῳ㑌 ක㸝 㸝ᰬ㸞ᯐཋ⏍∸໩Ꮥ◂✪ᡜ◂✪ࢬࣤࢰ࣭⢶㈻ ◂✪㒂㛓㸞

ᗑ㛏 ⚗⏛ ᜠῺ㸝㸝ᰬ㸞ᯐཋ⏍∸໩Ꮥ◂✪ᡜ㸞

≁ืㅦⁿ㻋㻔㻗㻝㻕㻘䡐㻔㻗㻝㻘㻘㻌㻃

ࠔᮄᮇ᳔∸࠾ࡼࡡ⏍⌦Ὡᛮ∸ ㈻ࡡฦ㞫ࠕ

㯦ᰏ ḿ඼㸝┬❟ᗀᓞኬᏕ⏍࿤⎌ሾᏕ㒂㸞

ᗑ㛏 ᩺⨶ ၻ⾔㸝┬❟ᗀᓞኬᏕ⏍࿤⎌ሾᏕ㒂㸞

ୌ⯙ㅦⁿ䟺㻔㻘㻝㻔㻘䡐㻔㻚㻝㻖㻓䟻㻃

㸝&ఌሔ㸯 ୯ㅦ⩇ᐄࠉ'ఌሔ ୯ㅦ⩇ᐄࠉ(ఌሔ ୯ㅦ⩇ᐄ㸞

᠋ばఌ䟺㻔㻚䠌㻗㻘䡐㻔㻜䠌㻓㻓䚮ఌሔ䠌┬❟ᗀᓞኬᏕᗀᓞ䜱䝧䝷䝕䜽㣏ᇸ䟻㻃

ུ㈳ㅦⁿ࣬≁ืㅦⁿ࣬フ㆗ဤఌ࣬࣬࣬ ኬㅦ⩇ᐄ

ୌ⯙ㅦⁿ &ఌሔ࣬࣬࣬ ୯ㅦ⩇ᐄ 'ఌሔ࣬࣬࣬ ୯ㅦ⩇ᐄ (ఌሔ࣬࣬࣬ ୯ㅦ⩇ᐄ

᠋ばఌ࣬࣬࣬࣬࣬࣬࣬࣬㣏ᇸ

ᙲဤఌ࣬࣬࣬࣬࣬࣬࣬࣬ᩅ⫩◂✪Ჯ㸦 㝭 ఌ㆗ᐄ

ኬㅦ⩇ᐄ

㣏ᇸ

&ఌሔ ఆ៹ᐄ

'ఌሔ (ఌሔ

3 

ୌ⯙ㅦⁿࣈࣞࢡ࣑ࣚ㸝Ⓠ⾪ ฦ㸡㈻␪㸧ฦ㸞

㸱&

ఌሔ㸯 ୯ㅦ⩇ᐄ㸳

ᗑ㛏 ௯ᏺ᰷ ⷽ㸝㎾␝ኬᕝ࣬⏍໩㸞

15:15 㹳

A 1

Vibrio choleraeࢣࢿ࣑ࡡࣝࢹࣞࣤ㡷ᇡ࡞࠽ࡄࡾኣᵕᛮ࡛᩺ぞmsDNAࡡᵋ㏸よᯊ ې஬୕ ྀ⨶Ꮔ^㸦ࠉ㔕㑒 ⨶Ṅ^㸦ࠉ▴⏛ Ὂ஦㑳^㸦ࠉ⠓⏛ ⣟⏠^㸧ࠉᓞᮇ ᩒ^㸦 㸝^㸦ᗀᓞኬ㝌࣬⏍∸ᅥ࣬㣏ဗ⾠⏍㸡^㸧ᒱᒜ⌦ኬ࣬⌦࣬⮣ᗃ⏍࿤㸞

15:27 㹳

A 2

ይ⇍Ⳟ⏜ᮮࣄ࢛ࢲࣤ⏍ྙᠺ⣌㓕⣪࡞㛭ࡌࡾ◂✪̽9KHUPXV WKHUPRSKLOXV⏜ᮮ0&5&ྙ ᠺ㓕⣪ࡡ㐿ఎᏄ᥀⣬࡛㓕⣪Ὡᛮ᳠ฝ̽

ې❉⏛ 㧏⚽ࠉୖ㔕 ᝙ᖲࠉ࿰ἠ ይゝ 㸝㫵ཱིኬᕝࠉ⏍ᚺᕝ㸞

15:39 㹳

A 3

᚜⏍∸࡞࠽ࡄࡾࢬࣝࣤ㓗㑇ඔ࡞㛭୙ࡌࡾ㐿ఎᏄࡡ᥀⣬࡛よᯊ

㛓⬝ ⏜ᕵᏄࠉ℀⏛ ᑠ྿ࠉې㜨ཾ ฺᩝ

㸝┬❟ᗀᓞኬ࣬⎌ሾ⛁Ꮥ㸞

15:51 㹳

A 4

&HLILWKLRGFHLOOXVIHUURR]LIFQVࡡ୹さ࡝࣭࣎ࣛࣤࢅࢤ࣭ࢺࡌࡾ㐿ఎᏄRPSࡡⓆ⌟࡞

ཀྵ࡯ࡌ⎌ሾᅄᏄࡡᙫ㡢

ې࣓ࣀࢴ࣒ࢺ ࣏ࣤࢨࣖࣜࠉ㔘ᑹᚽⰶࠉᮙᑹ ๙ࠉ୕ᮟୌ㞕 㸝ᒱᒜኬ㝌࣬⮤↓࣬ࣁ࢕࢛㸞

ᗑ㛏 㜨ཾ ฺᩝ㸝┬❟ᗀᓞኬ࣬⎌ሾ⛁Ꮥ㸞

16:03 㹳

A 5 Functional Role of Domain III of Cry Insecticidal Proteins from Bacillus thuringiensis ې Mohammad Tofazzal Hossain Howlader, Yumiko Ishida, Akiko Nakaguchi, Keiko Oka,

Kouji Ohbayashi, Masashi Yamagiwa, Tohru Hayakawa, and Hiroshi Sakai (Graduate School of Natural Science and Technology, Okayama University)

16:15 㹳

A 6

Shewanella violaceaࢨࢹࢠ࣑ࣞc₅࡞࠽ࡄࡾࢩࢪࣜࣆ࢔ࢺ⤎ྙࡡᙲ๪

ې➁୯ ⪯ࠉ୔ᮇᮄ ⮫Ᏻ 㸝ᗀኬ㝌⏍∸ᅥ㸞

16:27 㹳

A 7

ฦ⿛㓕ẍࡡ々ᩐ⭯㈇㏳ࢰࣤࣂࢠ㈻ᑻᅹ࡞࠽ࡄࡾࢪࢷ࣭ࣞࣜࡡ㔔さᛮ

ᒷᇖ ▩Ꮔࠉې➁ᕖ ⷽ 㸝㤮ᕖኬ࣬㎨࣬ᚺ⏕⏍∸㸞

16:39 㹳

A 8

^{ฝⰾ㓕ẍ࡞࠽ࡄࡾ}(*3-.8㛣ࡡ㒂న≁␏Ⓩ⤄ᥦ࠻ࡡຝ⋙୕᪴ን␏ᰬࡡよᯊ

ېᰏᮇ ᨼᙪࠉᮿᓧ ᾀ᪺ࠉ⛑㔕 ⌮஄

㸝⚗ᒜኬ࣬⏍࿤ᕝ࣬⏍∸ᕝ㸞

ᗑ㛏 ᓞᮇ ᩒ㸝ᗀᓞኬ㝌࣬⏍∸ᅥ࣬㣏ဗ⾠⏍㸞

16:51 㹳

A 9

᪝ᮇᾇ῕ᾇ⎌ሾ࠾ࡼࡡ᭯⏕᚜⏍∸ࡡฦ㞫

⚗ᮇ ೸ᾀࠉې௯ᏺ᰷ ⷽ 㸝㎾␝ኬᕝ࣬⏍໩ᕝ㸞

17:03 㹳

A 10

^{㧏ᗐይሲཿ⣵Ⳟ}-FORFUHXOFMFSRQLHF73&࣒࣭࣎ࣛࣚࢭධࢦࣇࣗࢼࢴࢹࡡኬ㔖Ⓠ⌟ཀྵ

ࡦࢦࣇࣗࢼࢴࢹ㛣┞பష⏕ࡡよᯊ ېᮿ࿝ ᘧஒࠉ⚗ᮇ ᐰ᪺ࠉ௯ᏺ ᰷ⷽ

㸝㎾␝ኬᕝ࣬⏍໩㸞

5 

㸱'

ఌሔ ୯ㅦ⩇ᐄ㸳

ᗑ㛏 ఫࠍᮄ ೸㸝ᗀᓞᅗ㝷Ꮥ㝌ኬᏕ ࣁ࢕࢛࣬ࣛࢦ࢕ࢠࣜᏕ⛁㸞

15:15 㹳

B 1

࢞ࢗ࢕ࣆ࣭ࣜࢵ࡞࠽ࡄࡾ⣵⬂ባ௥ㅨ࡞࠾࠾ࢂࡾ㓕⣪㜴ᐐࢰࣤࣂࢠ㐿ఎᏄࡡⳞ࢙ࣛࢨࢰ࣭

ᚺ➽

ېᶣᮇ ࿰៿ࠉථ⯢ ᾀᖲ (┬❟ᗀᓞኬ࣬⏍࿤⎌ሾ)

15:27 㹳

B 2

ẴᏇࡡⓆ㐡࡞㛭ࢂࡾࢨࣞ࢕ࢽࢻࢫࢻ㐿ఎᏄࡡⓆ⌟โᚒ㡷ᇡࡡよᯊ

ې㉝ᆊ ⿩ஒࠉᕖྡྷ ㄌࠉ୯ᕖ ᙁ 㸝ᓞ᰷ኬ࣬⥪⛁ࢬࣤࢰ࣭ࠉ^㸧⏍∸㈠″㸞

15:39 㹳

B 3

^{࢞ࣔࣅ࣭ࣚࣛᆵᏽ㔖5(7}⿞⨠࡞ࡻࡾ㐿ఎᏄ⤄ᥦ࠻ࢹ࣓ࢗࣞࢤࢨࡡᏽ㔖᮪௲ࡡ᳠ゞ

ې㇇⏛ ᏭᇱỜ^㸦ࠉ✕ᒜ ᾀ^㸧ࠉᮙᮟ කỄ^㸦ࠉᆊ⏛ ࡆࡍ࠻^㸧ࠉཿ஬ ⪵^㸨ࠉᶪ⏛ ࿰⨶^㸨ࠉ Ờᆊ ᏺ᫋^㸩ࠉ⡷ㆺ Ằ㞕^㸧

㸝^㸦ᗀᓞ┬❟⥪ᢇ◂ಕ⎌ࢬࠉ^㸧ᅗ❟⾠◂ࠉ^㸨㣏⥪◂ࠉ^㸩ᗀᓞኬ㝌⏍∸ᅥ⛁Ꮥ㸞

ᗑ㛏 ථ⯢ ᾀᖲ㸝┬❟ᗀᓞኬ࣬⏍࿤⎌ሾ㸞

15:51 㹳

B 4

ࢨࣞ࢕ࢽࢻࢫࢻ࡞࠽ࡄࡾ࢓ࣇࢨࢩࣤ㓗ࠉࢩࣔࢪ࣓ࣤ㓗࣒ࢲࣜㄇᑙẴᏇ㛚ཾࢨࢡࢻࣜఎ㐡

⤊㊪̿ࣈࣞࢷ࢕ࣤ࣌ࢪࣆ࢒ࢰ࣭ࢭ 㸶ࡡᙲ๪̿

ې㰳⸠ ├Ẇࠉ୯ᮟ ᏿╡ࠉୖ▴ 㟱᫓ࠉᮟ⏛ ⰶ⾔

㸝ᒱᒜኬᏕኬᏕ㝌⮤↓⛁Ꮥ◂✪⛁㸞

16:03 㹳

B 5

Antioxidant defense mechanisms of proline and glycinebetaine in tobacco suspension cells against salt stress-induced oxidative damage

ېMd. Anamul Hoque, Mst. Nasrin Akhter Banu, Eiji Okuma, Katsumi Amako*, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

Graduate School of Natural Science and Technology, Okayama University, *Faculty of Nutrition, Kobe Gakuin University㸞

16:15 㹳

B 6

᳔∸ࡡࣈࣞࣛࣤ⏍ྙᠺ࣬௥ㅨ⤊㊪ࡡよ᪺࡛⪇ሲᛮ⋋ᚋ࡞㛭ࡌࡾ◂✪

ې㧏ᶣ ⿩᫓ࠉ᳔ᆀ ὊᏄࠉ❟▴ ⬗ᏄࠉỜᆊ ᏺ᫋

㸝ᗀኬ㝌࣬⏍∸ᅥ⛁Ꮥ◂✪⛁㸞

16:27 㹳

B 7

ࢰࣁࢤ࡞࠽ࡄࡾ࢓ࢪࢤࣜࣄࣤ㓗⏍ྙᠺ⤊㊪࡛⏍⌦ᶭ⬗ࡡよ᪺

ېᆊᮇ ┷྿ࠉ⚗Ễ ୌᠺࠉỜᆊ ᏺ᫋

㸝ᗀኬ㝌࣬⏍∸ᅥ⛁Ꮥ◂✪⛁㸞

ᗑ㛏 ᲻Ễ ງ㸝┬❟ᗀᓞኬ࣬⏍࿤⎌ሾ㸞

16:39 㹳

B 8

^{㉰ይ⇍࢓࣭࢞࢓}8XOIRORGXVWRNRIFLLVWUFLQ ⏜ᮮ1࢓ࢪࣂࣚ࢟ࣤ㓗࢛࢞ࢨࢱ࣭ࢭࡡ=

⥲⤎ᬏᵋ㏸よᯊ

ېὰ஬ ୌష^㸦ࠉ⡷⏛ ୌᠺ^㸦ࠉḀᗖ ᫋ᙢ^㸦ࠉኬᓞᨼ஁^㸧

㸝^㸦ᚠᓞኬ㝌࣬ࢮࢨ࢛ࢷࢠࢿࢦ࢕࢙ࣤࢪ◂✪㒂ࠉ^㸧ஐᕗኬ㝌࣬㎨Ꮥ◂✪㝌㸞

16:51 㹳

B 9

㕪㗨ࢪࣚࢡࢅ⏕࠷ࡒකྙᠺ⣵Ⳟ࡞ࡻࡾỀ㈻Ὧ໩࡛⌓㓗ሲࡡ⁈ฝ

㹄⣵ᕖ 㞕ୌࠉᕖᮇ బኯࠉ➁㔕 ೸ḗࠉఫࠍᮄ ೸ࠉ㐪⸠ ᨼ㑳 㸝ᗀᓞᅗ㝷Ꮥ㝌ኬᏕ ࣁ࢕࢛࣬ࣛࢦ࢕ࢠࣜᏕ⛁㸞

17:03 㹳

B 10

Ⓠක᚜⏍∸ᅖᏽ໩ࢲࢴࣈࢅ⏕࠷ࡒ࢛ࣤࢦ࢕ࢹ'4) ゝῼ

ې‹ཾ Ᏻ᪺ࠉᒜᓧ ┷༡ࠉ㜨ཾ ฺᩝ

㸝┬❟ᗀᓞኬ࣬⎌ሾ⛁Ꮥ㸞

17:15 㹳

B 11

࢜࢞Ṿ࢓ࢠ࢓࣭࣍ࣜ࡞ࡻࡾఴ๨Ὡᛮỗἶࡡ΅ᐖ

㐪⸠ ╤ᕦ{ࠉ➁㔕 ೸ḗ{ࠉఫࠍᮄ ೸{ࠉᴃ ᨼ᪺t

㸝^㸦ᗀᓞᅗ㝷Ꮥ㝌ኬᏕ ࣁ࢕࢛࣬ࣛࢦ࢕ࢠࣜᏕ⛁㸰^㸧㸝᭯㸞࢓ࢠ࢓ࢷࢠࢿࢪ㸞

7 

㸱(

ఌሔ ୯ㅦ⩇ᐄ㸳

ᗑ㛏 㛏ᑹ ์⏠㸝┬❟ᗀᓞኬ࣬⏍࿤⎌ሾ㸞

15:15 㹳

C 1

^{㭔༵Ⓣ࢓ࣜࣇ࣐ࣤࡡ8}໩㸝⇍Ꮽᏽ໩㸞ᶭᵋࡡよ᪺

ې▴୷ 㝧⾔ࠉ௿⸠ ୌᠺࠉᮿෞ ├ฺ

ᒜཾኬ࣬㎨࣬⏍∸ᶭ⬗

15:27 㹳

C 2

12HWKLRQLQHȚ1^FVHࡡὩᛮ୯ᚨṟᇱ(^V࡫ࡡን␏ᑙථ࡞ࡻࡾᇱ㈻≁␏ᛮࡡᨭን ېᕝ⸠ ኬⶮࠉ⏛ᮟ 㝧ࠉᒺ ┷ኯ㑳ࠉ℟ᮇ ᪺⏍ࠉ⛼ᇄ ㈶஦

㸝ᒱᒜኬ㝌࣬⮤↓⛁Ꮥࠉሲ㔕⩇⿿ⷾ㸞

15:39 㹳

C 3

ࣃࢹኬ⭘⒬⣵⬂ᰬ࡞࠽ࡄࡾ⢋⭯࣑ࢲࣤMUCࡡⓆ⌟࡞ཀྵ࡯ࡌ㣏ဗ᢫ฝᾦࡡᙫ㡢

Ⓣ♼ ಆᖶ

㸝ࢿ࣭ࢹࣜࢱ࣑ΰᚨዥᏄኬ࣬ெ㛣⏍Ὡ࣬㣏ဗᰜ㣬㸞

ᗑ㛏 ྚ㔕 ᬓ஄㸝┬❟ᗀᓞኬ࣬⏍࿤⎌ሾ㸞

15:51 㹳

C 4

≁␏Ⓩ⣵⬂᥾ഭ⺦Ⓣ㈻ 22N)ࡡష⏕ᶭᵋ ུᐖమཀྵࡦ⣵⬂හᚺ➽ࡡ᥀⣬

ېᮿᮟ ♰௒ࠉᑚሪ ᪸ᘧࠉṂ㒂 ⪵ࠉ༐ ⳝኰࠉ᪡ᕖ ᚥࠉ㒿஬ ⿩ ᒱᒜኬ㝌࣬⮤↓⛁Ꮥࠉ㎾␝ኬ࣬⏍∸⌦ᕝࠉಘᕗኬ࣬㎨

16:03 㹳

C 5

㣏㣭ᛮࣄࢰ࣐ࣤ㸷6࡞ࡻࡽኬ⭘⤄⧂࡞࠽࠷࡙Ⓠ⌟ንິࡌࡾᅄᏄࡡ⥑⨮Ⓩ᥀⣬

ې㫵ᐓ ᅽ᜽ࠉኬ⏷ ᬓ⨶ࠉ♼⏛ ┷ᘢࠉ▦୯ ぞ஄ࠉຊ⸠ ⠂஁

(ᗀᓞኬ㝌㹺⏍∸ᅥ⛁Ꮥ)

16:15 㹳

C 6

DHA⤎ྙᆵࣛࣤ⬙㈻ࡡᢘ⾉ᑚᯀὩᛮ໩ᅄᏄ(PAF)ష⏕

ې㔘⏛ ㍜஄ࠉ஁ᒜ ᚥࠉ⩒⏛ ᑠᙢ 㸝ങ๑໩ᠺऒ㸞

ᗑ㛏 ▦୯ ぞ஄㸝ᗀᓞኬ㝌㹺⏍∸ᅥ⛁Ꮥ㸞

16:27 㹳

C 7

ࣆ࣭ࢺ࣎ࣛࣆ࢘ࢿ࣭ࣜࡡⷾ∸௥ㅨ㓕⣪ࡡష⏕࡞ཀྵ࡯ࡌᙫ㡢

ېᮄᮟ ཪ㤮ࠉ௿᮶ ⚵஄ࠉἴኣ㔕 ງ 㸝ᒱᒜኬ㝌࣬༈ṉⷾ㸞

16:39 㹳

C 8

࣎ࣛࣆ࢘ࢿ࣭ࣜ㢦ࡡᾐ໩⟮ྺ཭ິឺ࡛ᢘ㓗໩ὩᛮⓆ⌟ࡡフ౮

ېཋᮇ ┷㔓ࠉ㔕ᆀᮇ ࿰Ꮟ¹ࠉ⏍㥎 ᬓᏄ¹ࠉṂ⸠ ᚠ⏠

(┬❟ᗀᓞኬ㝌࣬⥪ྙᏕ⾙ࠉ¹ᗀᓞ┬ኬ࣬⏍∸㈠″)

16:51 㹳

C 9

ΰ㒿㔂㏸᫤࡞ふᐳࡈࡿࡾ㓕ẍ࣐ࢹࢤࣤࢺࣛ࢓ࡡᙟឺን໩ࡡណ⩇ࡡよᯊ

ې໪ᇄ ᾀᚷࠉⲠᮄ ⩇㞕ࠉ⯢ᡖ ⪌ୌࠉୖ㣜 ொ 㸝㸝≺㸞㒿⥪◂ࠉᗀኬ㝌⏍∸ᅥ⛁Ꮥ◂✪⛁㸞

17:03 㹳

C 10

᚜⏍∸ንᥦ࡞ࡻࡾ࢛࣭ࣛࣇ஦ḗ௥ㅨ⏐∸ࡡ㧏ᶭ⬗໩

ې♼ᓧ ᾀࠉཋ⏛ Ⴢᗀ^㸟ࠉᑚᯐ ஁⨶^㸟ࠉொᡖ⏛ ↯ᙢ 㸝ᒱᒜኬ㝌⮤↓㸡^㸟ᒱᒜኬ㎨㸞

ུ㈳ㅦⁿ࣬≁ืㅦⁿ

ㅦ ⁿ さ ᪠

ᖳᗐ㎨ⰹ໩Ꮥዜທ ㈳ུ㈳ㅦⁿ

කྙᠺ⏍∸࡞࠽ࡄࡾ࢓ࢪࢤࣜࣄࣤ㓗࢛࣋ࣜ࢞ࢨࢱ࣭ࢭ ࡡⓆ⌟ㄢ⟿ᶭᵋ࡛⏍⌦ᶭᵋࡡよ᪺

ᓞ᰷ኬᏕ⏍∸㈠ ″⛁Ꮥ㒂⏍࿤ᕝᏕ⛁ ▴ᕖᏏ༡

㓗⣪Ⓠ⏍ᆵࡡකྙᠺࢅ⾔࠹᳔∸ࡷ⸬㢦ࡢࠉ⣵⬂හ㓗⣪⃨ᗐ࠿㟸ᖏ࡞㧏ࡂὩᛮ㓗⣪⛸

㸝 748㸞࠿ᐖ᪾࡞⏍ᠺࡌࡾࠊࡐࡡࡒࡴකྙᠺ⏍∸ࡢ≺⮤ࡡ 748 ௥ㅨ⣌࡛ࡊ࡙࢓ࢪࢤࣜࣄ

ࣤ㓗ࢡࣜࢰࢲ࢛ࣤࢦ࢕ࢠࣜࢅⓆ㐡ࡈࡎࠉ 748 ⃨ᗐࢅཚᐠ࡞ㄢ⟿ࡊ࡙࠷ࡾࠊ࢓ࢪࢤࣜࣄ

ࣤ㓗࢛࣋ࣜ࢞ࢨࢱ࣭ࢭ㸝 &5=㸞ࡢࠉࡆࡡࢦ࢕ࢠࣜࡡ㘵㓕⣪࡛ࡊ࡙⣵⬂හ -

4

௥ㅨࡡ୯ᚨ

Ⓩᙲ๪ࢅᢰࡖ࡙࠷ࡾࠊᮇ◂✪࡚ࡢකྙᠺ⏍∸ࡡ &5= ࢅ㐿ఎᏄ࡚ࣝ࣊ࣜよᯊࢅ㐅ࡴࠉࡐ ࡡ⏍⌦ᶭ⬗ࡡ よ᪺ࢅ⾔ࡖࡒࠊ௧ୖ࡞ࡐࡡᴣ さࢅ㏑࡬ࡾࠊ

㸦㸣 &5= ࡡࢠ࣭ࣞࣤ໩࡛කྙᠺ⏍∸㛣࡚ࡡฦᕱ

࣭ࣗࢡࣝࢻ &5= ࡞ᑊࡌࡾ࣓ࢿࢠ࣭ࣞࢻࣜᢘమࢅࣈ࣭ࣞࣇ࡛ࡊ᳔࡙∸࡛⸬㢦࠾ࡼ &5= ࡡ ࢠ࣭ࣞࣤ໩࡞ᠺຉࡊࠉකྙᠺ⏍∸࡞࠽ࡄࡾ &5= ࡡฦᕱࢅ㐿ఎᏄⓆ⌟࡚᪺ࣝ࣊ࣜࡼ࠾࡞

ࡊࡒࠊ᳔∸࡚ࡢⴝ⥫మࠉ࣐ࢠࣞ࣍ࢸ࢔࣭ࠉ⣵⬂ ㈻࡞࢓࢕ࢮࢧ࢕࣑࠿ᑻᅹࡌࡾࡡ࡞ᑊࡊࠉ

⸬㢦࡚ࡢ࢓࢕ࢮࢧ࢕࣑ࡢᏋᅹࡎࡍ࣭ࣗࢡࣝࢻࡷࢠ࡚ࣞࣝࣚࡢ⣵⬂㈻ ࡞ࠉࢠ࣐ࣚࢺ࣓ࢻ ࢪ࡚ࡢⴝ⥫మࡡࢪࢹ࣏ࣞࡡࡲ࡞ᑻᅹ࠿㝀ࡼࡿ࡙࠷ࡒࠊ&5= ࡡฦᕱࡢࠉකྙᠺ⏍∸㛣࡞

࠽ࡄࡾ -

4

௥ㅨᶭᵋࡡ㐢࠷࡛ᐠ᥃࡞㛭౿ࡊ࡙࠷ࡾࡆ࡛࠿᪺ࡼ࠾࡞࡝ࡖࡒࠊ

㸧㸣㐽ᢝⓏࢪࣈࣚ࢕ࢨࣤࢡ࡞ࡻࡾⴝ⥫మᆵ &5= Ⓠ⌟ㄢ⟿ᶭᵋࡡⓆぜ

࣌ࢗࣝࣤࢮࢗⴝ⥫మ࡞ᑻᅹࡌࡾࢲࣚࢤ࢕ࢺ⭯⤎ྙᆵ &5=W&5=࠽ࡻࡦࢪࢹ࣏ࣞྊ⁈ᆵ

&5= ࡢࠉ̓ᮆ ❻㡷ᇡࡡ㐽ᢝⓏࢪࣈࣚ࢕ࢨࣤࢡ࡞ࡻࡽⓆ⌟ㄢ⟿ࢅུࡄࡾࡆ࡛ࢅⓆぜࡊࡒࠊ

㐿ఎᏄ&5=..୕ࡡ㐽ᢝⓏࢪࣈࣚ࢕ࢨࣤࢡ࡞㛭ࢂࡾࢨࢪ㡷ᇡྜྷᏽ࡞ᠺຉࡌࡾ࡛࡛ࡵ࡞ࠉ ࢹࣚࣤࢪᅄᏄ࡛ࡡ ┞பష⏕よᯊ࠾ࡼࠉࡆࡡ㐽ᢝⓏࢪࣈࣚ࢕ࢨࣤࢡࡢකྙᠺ⤄⧂≁␏Ⓩ

࡝ W&5= ࡡ⏐⏍࡞ᶭ⬗ࡊ࡙࠷ࡾࡆ࡛ࢅよ ᪺ࡊࡒࠊ

㸨㸣ᙟ ㈻㌹ᥦ᳔∸ࢅ⏕࠷ࡒ &5= ࢓࢕ࢮࢧ࢕࣑ࡡ⏍⌦ᶭ⬗ フ౮

W&5= 㐛๨Ⓠ⌟ࢰࣁࢤ㸝9S9&5㸞ࡢ㢟ⴥ࡝ක࣬㓗໩ࢪࢹࣝࢪ⪇ᛮࢅ♟ࡊࡒࠊ㔕⏍ᰬ࡛

ࡡẒ㍉࡞ࡻࡽࠉක࣬㓗໩ࢪࢹࣝࢪ㝸ᐐࡡิ᭿ さᅄࡢⴝ⥫మ୯ࡡ࢓ࢪࢤࣜࣄࣤ㓗ྱ㔖ࡡ

఩ୖ࡛ⴝ⥫మᆵ &5= ࡡ኶Ὡ࡞࠵ࡾࡆ࡛ࢅᥞ၌ࡊࠉ 9S9&5 ࡡ⪇ᛮ⬗ࡢࢪࢹࣝࢪิ᭿ṹ 㝭࡚኶Ὡࡊࡒⴝ⥫మᆵ &5= ࡡ┞⿭࡞ࡻࡽ⋋ᚋࡈࡿࡒࡆ࡛ࢅ᪺ࡼ࠾࡞ࡊࡒࠊ

⣵⬂ ㈻ᆵ &5= ࡡ⏍⌦ᶭ⬗࡞ࡗ࠷࡙ࡵ᩺ࡒ࡝▩ぜࢅᚋࡒࠊ &5= Ὡᛮࢅ㔕⏍ᰬࡡ⣑ ࡱ࡚

఩ୖࡈࡎࡒ⣵⬂ ㈻ᆵ &5= Ⓠ⌟ᢒโࢰࣁࢤᇰ㣬⣵⬂ࡢࠉ⇍ࡷሲฌ⌦࡞ࡻࡾ⎌ሾࢪࢹࣝࢪ

࡞ᑊࡊ࡙ⴥࡊ࠷⪇ᛮ⬗ࡡቌᙁ࠿ヾࡴࡼࡿࡒࠊࡐࡡさᅄࡢࣝࢺࢴࢠࢪᚺ➽ᛮ࢞ࢻ࣭ࢭࡡ Ὡᛮ໩࠽ࡻࡦࢪࢹࣝࢪᚺ➽ᛮ㐿ఎᏄ⩄ࡡⓆ⌟୕᪴࡞࠵ࡾࡆ࡛࠾ࡼࠉ⣵⬂ ㈻ᆵ &5= ࡢ⣵

⬂හࣝࢺࢴࢠࢪࢨࢡࢻࣜㄢ⟿ࢅ௒ࡊࡒࢪࢹࣝࢪᚺ➽ᛮ࡞ࡵᶭ⬗ࡊ࡙࠷ࡾࡆ࡛ࢅᥞ၌ࡊ

ࡒࠊ

10 

ᖳᗐᨥ㒂ዜທ ㈳ུ㈳ㅦⁿ

⥲⹰ࡡఆ┸࣬ᑋ࿤โᚒᶭᵋ࡞㛭ࡌࡾ໩Ꮥ⏍∸ᏕⓏ◂✪

㫵ཱིኬᏕ㎨Ꮥ㒂 ἑ㔕 ᙁ

࠘⫴ᬊ࡛┘Ⓩ࠙⥲⹰

(FHQRUKFGILWLVHOHJFQV

ࡢ⏍⫩⎌ሾࡡᝇ໩࡞ᚺ➽ࡊ࡙ୌ᫤Ⓩ࡞⏍

⫩ࢅೳḾࡊᖺ⹰᭿࡚ఆ ┸ࡌࡾࠊ⎌ሾさᅄࡡ㸦ࡗ࡛ࡊ࡙⥲⹰࠿ฦἢࡌࡾఆ ┸ㄇᑙ∸

㈻ ࠿▩ࡼࡿ࡙࠷ࡾࠊࡱࡒ9,+

β

ᵕࢨࢡࢻࣜ࡝ࡼࡦ࡞࢕ࣤࢪࣛࣤᵕࢨࢡࢻࣜ࠿ࡆࡡᖺ

⹰ఆ ┸ࢅโᚒࡌࡾࡆ࡛࠿᪺ࡼ࠾࡞࡝ࡖ࡙࠷ࡾࠊࡈࡼ࡞ࡆࡡ࢕ࣤࢪࣛࣤᵕࢨࢡࢻࣜࡢ

ᠺ⹰ᑋ࿤ࢅโᚒࡌࡾࡆ࡛࠿ึ᪺ࡊ࡙࠽ࡽ⌟ᅹ࡚ࡢ࢕ࣤࢪࣛࣤᵕࢨࢡࢻࣜ࡞ࡻࡾᑋ࿤

โᚒࡢິ∸⛸࡞භ㏳࡚࠵ࡾࡆ࡛࠿▩ࡼࡿ࡙࠷ࡾࠊ

ᠻࠍࡢ⥲⹰࠿⏐⏍ࡌࡾ∸ ㈻ࢅᶭ㍂ ࡛ࡊ࡙ఆ┸࣬ᑋ࿤โᚒࡡࠔථງᶭᵋࠕࢅ᪺ࡼ࠾࡞

ࡌࡾࡆ࡛ࢅ┘ Ⓩ࡛ࡊ࡙࢕ࣤࢪࣛࣤᵕฦᏄࡡྜྷᏽ࣬ᶭ⬗よᯊࢅ⾔࠹࡛භ࡞ఆ┸ㄇᑙ

∸ ㈻ࡡ໩ᏕⓏ࣬⏍∸ᏕⓏ≁ᛮࢅ᪺ࡼ࠾࡞ࡊࡒࠊ

࠘࢕ࣤࢪࣛࣤᵕฦᏄࡡྜྷᏽ࠙ᠻࠍࡢ㐅⾔୯ࡡࢣࢿ࣑ࣈࣞࢩ࢘ࢠࢹ᝗ሒࡻࡽ࢕ࣤࢪࣛ

ࣤᵕ㐿ఎᏄࡡ㒼าࢅ᢫ฝࡊ7&(* Ἢ࡞ࡻࡽධ㛏 H)3& ࡡࢠ࣭ࣞࢼࣤࢡࢅ⾔ࡖࡒ

㸝 (HLQVXOLQ㸞 ࠊ ࡈࡼ࡞᥆ᏽ࢓࣐ࢿ㓗㒼าࡻࡽ㧏ḗᵋ㏸ࢅ࣓ࢸࣛࣤࢡࡊဳ

஘ິ∸ࡡ࢕ࣤࢪࣛࣤ .,+. ࡛ࡡ㧏ḗᵋ㏸࡛Ẓ㍉ࡊࡒࠊ ུᐖమヾㆉ㟻ࡡẒ㍉࡞ࡻࡽ⥲

⹰ࡡ࢕ࣤࢪࣛࣤᵕฦᏄ࡛ဳ஘ິ∸ࡡࡵࡡ࡛ࡢஹཨὩᛮ࠿࡝࠷ࡆ࡛࡝࡜ࢅ♟ြࡊࡒࠊ

࠘ᶭ⬗ よᯊ࠙73& ᖱῦ࡝ࡼࡦ࡞㐿ఎᏄ◒ቪ࡞ࡻࡽ୕オ ࢕ࣤࢪࣛࣤᵕ㐿ఎᏄࡡᶭ⬗よᯊ

ࢅ⾔ࡖࡒࠊఆ ┸ㄇᑙ∸㈻ 㸝ୖオཤ↯㸞Ꮛᅹୖ࡞࠽ࡄࡾఆ ┸⋙࡝ࡼࡦ࡞ᑋ࿤ᘇ㛏᮪௲ୖ

࡚ࡡᠺ⹰ᑋ࿤ࢅῼᏽࡊ୕ オ࢕ࣤࢪࣛࣤᵕฦᏄࡢ࢓ࣤࢰࢥࢼࢪࢹ࡛ࡊུ࡙ᐖమࢨࢡࢻ

ࣜࢅ΅ᑛࡈࡎఆ ┸࡝ࡼࡦ࡞ᑋ࿤ᘇ㛏ࢅㄇᑙࡌࡾྊ⬗ᛮࢅ♟ࡊࡒࠊࡆࡿࡢ࢕ࣤࢪࣛ

ࣤᵕฦᏄ࠿࢓ࣤࢰࢥࢼࢪࢹ࡛ࡊ࡙ᶭ⬗ࡌࡾࡆ࡛ࢅ♟ࡊࡒ᭩ิࡡౚ࡚࠵ࡾࠊࡱࡒ OL [LQJ ,+5 Ἢ࡞ࡻࡽ᫤✭㛣ⓏⓆ⌟ࣂࢰ࣭ࣤࡡንິࢅよ ᯊࡊ᭩ࡵᙁງ࡝࢓ࣤࢰࢥࢼࢪ

ࢹ࡚࠵ࡾ (HLQVXOLQ ࡡ⭙㒂♼⤊⣵⬂࡞᪂ࡄࡾⓆ⌟࠿ᑋ࿤โᚒ࡞㔔さ࡚࠵ࡾྊ⬗ᛮࢅ

♟ࡊࡒࠊࡈࡼ࡞ (HLQVXOLQ ࡡ⭙㒂࡚ࡡⓆ⌟ࡢ )&++4=4 ㌹෕ᅄᏄ࡞౪Ꮛࡌ

ࡾࡆ࡛ࢅ♟ࡊࡒࠊ

࠘ఆ ┸ㄇᑙ∸ ㈻ࡡ≁ᛮ࠙ᠻࠍࡢ⥲⹰࠿మአ࡞ฦἢࡌࡾఆ┸ㄇᑙ∸ ㈻ࡡ⢥⿿ࢅ㐅ࡴࡾ

㐛⛤࡚ฦἢ∸୯࡞ᠺ⹰ᑋ࿤ᘇ㛏Ὡᛮࢅぜ ฝࡊࡒࠊὩᛮ∸㈻ࡢฦᏄ㔖 ⛤ᗐࡡ㧏

ᴗᛮỀ⁈ᛮ∸ ㈻࡚࠵ࡾ࡛᥆ᏽࡈࡿࡾࠊᮇ⢊⢥⿿∸ࡢఆ ┸ㄇᑙὩᛮࡡࡲ࡝ࡼࡍᑋ࿤ᘇ㛏

Ὡᛮࢅ᭯ࡊ࡙࠽ࡽࡐࡡᑋ࿤ᘇ㛏Ὡᛮࡢ )&+ ࡞౪Ꮛࡌࡾࡆ࡛ࢅ᪺ࡼ࠾࡞ࡊࡒࠊ

2007 ᖳᗐᨥ㒂ዜທ ㈳ུ㈳ㅦⁿ

᳔∸⢶ࢰࣤࣂࢠ㈻⢶㙈ࡡ௥ㅨᶭᵋ࡛࢓ࣝࣜࢣࣤ⢶㙈ࡡᵋ㏸࣬ᶭ⬗ よᯊ

ᕖᓧ༈ኬ࣬⾠⏍ ๑⏛ ᜠ

ฦ໩ᠺ㛏୯ࡡ᳔∸⣵⬂࡞ࡢ㸡㐗㞫ᆵࡡ࢓ࢪࣂࣚ࢟ࣤ⤎ྙᆵ⢶㙈㸝

N-

ࢡࣛ࢜ࣤ㸞࠿

µM

⃨ᗐ࡚Ꮛᅹࡌࡾࡆ࡛࠿▩ࡼࡿ࡙࠷ࡾࠊᮇ◂✪࡚ࡢ㸡ࡆࡿࡼ㐗㞫

N-

ࢡࣛ࢜ࣤࡡᶭ⬗よ᪺

◂✪ࡡୌ⎌࡛ࡊ࡙㸡

(1)

࢕ࢾᇰ㣬⣵⬂୯ࡡ㐗㞫

N-

ࢡࣛ࢜ࣤཀྵࡦ⢶⺦Ⓣ ㈻⢶㙈ࡡᵋ㏸≁

ᛮ よᯊ㸡

(2)

⢶㙈㐗㞫ࢅྒྷࡾ࢙ࣤࢺ

-β-N-

࢓ࢬࢲࣜࢡࣜࢤࢦ࣐ࢼࢱ࣭ࢭ

(ENGase)

ࡡᶭ

⬗≁ᛮ よᯊ㸡㐿ఎᏄࡡྜྷᏽ࡛Ⓠ⌟⣌ࡡᵋ⠇㸡⣵⬂හཀྵࡦ⤄⧂ฦᕱよᯊࢅ⾔ࡖࡒࠊࡱࡒ㸡

᳔∸࢓ࣝࣜࢣࣤ࡞⤎ྙࡌࡾᢘཋᛮ

N-

ࢡࣛ࢜ࣤࡡඞ␷Ὡᛮࢅ᪺ࡼ࠾࡞ࡌࡾ┘Ⓩ࡚㸡࢓ࣝ

ࣜࢣࣤ⢶㙈ࡡᵋ㏸≁ᛮよ ᯊ࡛⣵⬂ᛮඞ␷࡞㛭ࢂࡾ⏍⌦Ὡᛮ࡞ࡗ࠷࡙ࡡ᳠⣬ࢅ⾔ࡖࡒࠊ

(1) イネ培養細胞に存在する遊離N-グリカンの構造特性解析ᴆ

㑇ඔᮆ❻࡞

GlcNAc1

ṟᇱ᭯ࡌࡾࣀ࢕࣏ࣤࢿ࣭ࢪᆵ⢶㙈࠿

µM

⃨ᗐ࡚⣵⬂හ࡞Ꮛᅹࡌ

ࡾୌ᪁㸡㑇ඔᮆ ❻࡞

GlcNAc2

ṟᇱࢅ᭯ࡌࡾ᳔∸ 々ྙᆵ⢶㙈࡛ࣀ࢕࣏ࣤࢿ࣭ࢪᆵ⢶㙈࠿

nM

⃨ᗐ࡚⣵⬂አ࡞ฦἢࡈࡿ࡙࠷ࡾࡆ࡛ࢅ᪺ࡼ࠾࡞ࡊࡒࠊࡆࡡࡆ࡛ࡢ㸡஦⛸㢦ࡡ⢶㙈 㐗㞫㓕⣪㸝

ENGase

࡛

PNGase

㸞࠿ᑻᅹሔᡜ࡛⏍⌦Ⓩណ⩇ࢅ␏࡞ࡊ࡙⢶㙈௥ㅨ࡞㛭୙ࡌ

ࡾࡆ࡛ࢅ♟ࡌࡵࡡ࡚࠵ࡖࡒࠊ

(2) イネ ENGase の機能特性，遺伝子同定，細胞内及び組織分布解析ᴆ

࢕ࢾᇰ㣬⣵⬂࠾ࡼ

ENGase

ࢅ⢥⿿ᚃ㸡

ESI-MS

ฦᯊ࡞ࡻࡽහ㒂࢓࣐ࢿ㓗㒼าࢅỬᏽ ࡊ㸡ࡐࡡ㒼า᝗ሒࢅࡵ࡛࡞ᮇ㓕⣪࡛㧏࠷┞ ྜྷᛮࢅ᭯ࡌࡾᶭ⬗ᮅ▩㐿ఎᏄ⏐∸ࢅ๪ࡽฝ ࡊࡒࠊ ルᙔ㐿ఎᏄࢅ

E. coli

࡞ࡻࡽⓆ⌟ࡈࡎࡒ࡛ࡆࢀ㸡

ENGase

Ὡᛮࢅ☔ヾࡌࡾࡆ࡛࠿

࡚ࡀ㸡᳔∸

ENGase

㐿ఎᏄࢅྜྷᏽࡌࡾࡆ࡛࡞ᠺຉࡊࡒࠊୌ᪁㸡ඞ␷⤄⧂᯹ⰅἪ࡞ࡻࡽ

ENGase

࠿࢕ࢾᐁ⏍⫿㍂ ୯ࡡฦ⿛⤄⧂ࡡ⣵⬂㈻හ࡞ᙁࡂⓆ⌟ࡊ࡙࠷ࡾࡆ࡛ࢅ᪺ࡼ࠾࡞

ࡊࡒࠊ௧୕ࡡ⤎ᯕࡢ㸡᩺⏍ࢰࣤࣂࢠ ㈻ࡡဗ ㈻⟮⌦⣌࡞࠽ࡄࡾ

ENGase

ࡡ㔔さ ࡝⏍⌦ᶭ

⬗ࢅ♟ြࡌࡾࡵࡡ࡛ᛦࢂࡿࡾࠊ

(3) 植物アレルゲンに結合する抗原性N-グリカンの免疫活性ᴆ

ࢪ࢟ⰴ⢂࢓ࣝࣜࢣࣤ

(Cry j 1, Jun a 1)

࡞⤎ྙࡌࡾᢘཋᛮ⢶㙈ࡡᵋ㏸≁ᛮ᳔࡛∸ᢘཋ

ᛮ⢶㙈ࡡඞ␷Ὡᛮ࡞ࡗ࠷࡙ࡡ よᯊࢅ⾔ࡖࡒࠊࡐࡡ⤎ᯕ㸡ࡆࡿࡼⰴ⢂࢓ࣝࣜࢣࣤ࡞ࡢࣜ

࢕ࢪ

a

࢙ࣅࢹ࣭ࣈࢅ᭯ࡌࡾ᳔∸ᢘཋᛮ⢶㙈࠿⤎ྙࡌࡾࡆ࡛ࢅ᪺ࡼ࠾࡞ࡌࡾ࡛࡛ࡵ࡞㸡

᳔∸ᢘཋᛮ⢶㙈࠿

Th2

⣵⬂ࡡ

IL-4

⏐⏍ࢅ᭯ណ࡞ᢒโࡌࡾࡆ࡛ࢅぜฝࡊࡒࠊ

ᮇ◂✪ࢅ⾔࠹࡞࠵ࡒࡽࡇᣞᑙ㡤࠷ࡒᒱᒜኬᏕኬᏕ㝌⮤↓⛁Ꮥ◂✪⛁ᩅ᤭ ᮄᮟྚఘ

඙⏍㸡ᒱᒜኬᏕኬᏕ㝌༈ṉⷾ⥪ྙ◂✪⛁෶ᩅ᤭ ᒱ㔕ක༡඙⏍㸡ࡂࡼࡊࡀష㝟ኬᏕㅦᖅ ᮄᮟ୒㔓Ꮔ඙⏍㸡 ࡐࡊ࡙⌟ᅹࡡ୕ྒྷ࡚࠵ࡾᕖᓧ༈⛁ኬᏕᩅ᤭ ኬᵫ๙ᕦ඙⏍࡞វㅨ⏞ࡊ

୕ࡅࡱࡌࠊ ࡐࡡ௙ኣࡂࡡභྜྷ◂✪⩽ࡡ᪁ࠍ࡞ࡵࡆࡡሔࢅ࠽ೇࡽࡊ࡙࠽ ♡⏞ࡊ୕ࡅࡱࡌࠊ

12 

2007 ᖳᗐᨥ㒂ዜທ ㈳ུ㈳ㅦⁿ

'FHLOOXV

ᒌ⣵Ⳟ⏜ᮮࡡ᩺ぞ⎌≟஫⢶⏍ᠺ㓕⣪࡞㛭ࡌࡾ◂✪

ᰬᘟఌ ♣ᯐཋ⏍∸໩Ꮥ◂✪ᡜ࣬◂✪ࢬࣤࢰ࣭࣬⢶㈻◂✪㒂㛓

ῳ㑌 ක

⃞⢂࠾ࡼ㓕⣪Ⓩ࡞⏍ᠺࡌࡾ᩺ぞ࢛ࣛࢥ⢶ࢅ᳠⣬ࡌࡾ㐛⛤࡚ࠉᮅ▩࢛ࣛࢥ⢶ࢅ⏍ᠺࡌ

ࡾὩᛮࢅᅰቫ⏜ᮮ⣵Ⳟ

'FHLOOXVHLUHXOFQV

&2 ᰬࡡᇰ㣬୕ΰ୯࡞ぜ ฝࡊࡒࠊᮅ▩࢛ࣛ

ࢥ⢶ࢅ༟㞫ࡊࠉᵋ㏸ࢅỬᏽࡊࡒ࡛ࡆࢀࠉ࣏ࣜࢹ࣋ࣤࢰ࢛࣭ࢪ࠿Ș ⤎ྙ࡚⎌≟໩ࡊ ࡒ᩺ぞ⎌≟஫⢶ࠉH^HOR̀ЍȘ

)

,OHSЍȘ

)

,OHSЍȘ

)

,OHSЍ Ș

)

,OHSЍȘ

)

,OHSЍb࡚࠵ࡖࡒࠊᮇ⢶㈻ ࢅ࢕ࢮࢦ࢕ࢠ࣏ࣞࣜࢹ࣋ࣤࢰ

࢛࣭ࢪ.(, ࡛࿤ྞࡊࡒࠊ

.(, ⏍ᠺ࡞㛭୙ࡌࡾ㓕⣪ࢅ⢥⿿ࡊࠉㅎᛮ㈻ࢅㄢ࡬ࡒࠊᮇ㓕⣪ࡢฦᏄ㔖 N)F 8)85&,*ࠉ᭩㐲 S-㹳ࠉ᭩㐲Ὼᗐ 㹳ΥࠉS- Ꮽᏽᛮ 㹳ࠉῺᗐᏭᏽᛮ

Υࡱ࡚P2 (F

Ꮛᅹୖ࡚ Υࡱ࡚࡚ࠉ㔔ྙᗐ ௧୕ࡡ࣏ࣜࢹ࢛ࣛࢥ⢶ࡷ⃞⢂࡞

ష⏕ࡊ .(, ࢅ⏍ᠺࡊࡒࠊ ᮇ㓕⣪ࡡ࣏ࣜࢹࣉࣈࢰ࢛࣭ࢪ࡫ࡡష⏕ࢅ⤊᫤Ⓩ࡞ふ ᐳࡊࡒ࡛

ࡆࢀࠉཬᚺิ᭿࠾ࡼ .(, ࡡ⏍ᠺ㸝⎌≟໩ཬᚺ㸞࡛࡛ࡵ࡞࣏ࣜࢹ࢛ࣛࢥ⢶༟నࡡ්㒼า ཬᚺ㸝ฦᏄ㛣 ㌹⛛ཬᚺ㸞࠿ࡲࡼࡿࡒࠊࡆࡿࡼࡡ⤎ᯕ࠾ࡼᮇ㓕⣪ࡢ᩺ぞ࡝ࢡࣜ࢜ࢿࢹࣚ

ࣤࢪࣆ࣭࢘ࣚࢭ࡚࠵ࡾࡆ࡛࠿᪺ࡼ࠾࡛࡝ࡽࠉ࢕ࢮࢦ࢕ࢠ࣏ࣞࣜࢹ࢛ࣛࢥ⢶ࢡࣜ࢜ࢿࢹ

ࣚࣤࢪࣆ࣭࢘ࣚࢭ.,9FVH࡛࿤ྞࡊࡒࠊ

.,9FVH ࡡୌḗᵋ㏸ࢅ᪺ࡼ࠾࡞ࡌࡾࡒࡴࠉᮇ㓕⣪㐿ఎᏄࢅࢠ࣭ࣞࢼࣤࢡࡊሲᇱ㒼าࢅ

Ửᏽࡊࡒࠊᮇ㓕⣪㐿ఎᏄࡢ GS ࠾ࡼ࡝ࡽࠉࢨࢡࢻࣜ࣋ࣈࢲࢺ ṟᇱࢅྱࡳ

࢓࣐ࢿ㓗࠾ࡼ࡝ࡾࢰࣤࣂࢠ ㈻ฦᏄ㔖 N)Fࢅࢤ࣭ࢺࡊ࡙࠷ࡾࡆ࡛࠿ࢂ࠾ࡖࡒࠊ ᮇ㓕⣪ࡡ࢓࣐ࢿ㓗㒼า୯࡞ࡢȘ࢓࣐࣭ࣚࢭࡷࢦ࢕ࢠࣞࢸ࢞ࢪࢹࣛࣤࢡࣜ࢜ࢿࢹࣚࣤ

ࢪࣆ࣭࢘ࣚࢭ㸝 (,9FVH㸞࡝࡜ࡡ㛣࡚ಕᏋࡈࡿ࡙࠷ࡾ ࣧᡜࡡභ㏳㡷ᇡ࠿Ꮛᅹࡊࠉᮇ㓕

⣪ࡢ͆Ș࢓࣐࣭ࣚࢭࣆ࢒࣐࣭͇ࣛ࡞ᒌࡌࡾࡆ࡛࠿♟ြࡈࡿࡒࠊ

⃞⢂㒂ฦฦよ∸ NJ ࠾ࡼ⣟ᗐ ࡡ .(, ࢅ NJ ㄢ⿿ࡊࠉLQ

[LWURᾐ໩ᛮࠉ

ຊ⇍Ꮽᏽᛮࠉ࣒࢕࣭ࣚࢺཬᚺᛮ࡝࡜ࢅㄢ࡬ࡒࠊࡐࡡ⤎ᯕࠉᮇ⢶㈻ࡢ㟸㑇ඔᛮ࡚࣒࢕ࣚ

࣭ࢺཬᚺࢅ♟ࡈࡍࠉ⪇⇍ᛮ࡞ඁࡿࡒ㞬ᾐ໩ᛮ⢶ ㈻࡚࠵ࡾࡆ࡛࠿ࢂ࠾ࡖࡒࠊࡱࡒࠉ⎌≟

ᵋ㏸ࢅࡵࡗᮇ⢶ ㈻ࡢࠉࢦ࢕ࢠࣞࢸ࢞ࢪࢹ࡛ࣛࣤྜྷᵕ࡞఩ฦᏄ໩ྙ∸ࡡໜ᥃ష⏕ࢅ᭯ࡊ

࡙࠽ࡽࠉ≁࡞㓉㓗࡞ᑊࡌࡾໜ᥃⬗࡞ඁࡿ࡙࠷ࡒࠊࡆࡡࡻ࠹࡝≁ᚡࢅ᭯ࡌࡾ .(, ࡢࠉ᩺

ぞ ⣪ᮞ࡛ࡊ࡙ኣ᪁㟻࡚ࡡ⏕㏭࠿᭿ᙽࡈࡿࡾࠊ

≁ ื ㅦ ⁿ

ᮄᮇ᳔∸࠾ࡼࡡ⏍⌦Ὡᛮ∸ ㈻ࡡฦ㞫

┬❟ᗀᓞኬᏕ⏍࿤⎌ሾᏕ㒂 㯦ᰏ ḿ඼

ཿࡂ࠾ࡼ᳔∸ࡢࣀ࣭ࣇ࠵ࡾ࠷ࡢ⏍࡛ⷾࡊ࡙ெ㢦ࡡ⏍Ὡࡷ⑄⑋἖⒢ ࡞ᗀࡂ⏕࠷ࡼࡿ࡙

ࡀࡒࠊࡐࡿࡢ᳔∸࠿ኣᙤ࡝㸧ḗ௥ㅨ⏐∸ࢅ⏍ྙᠺࡊ᳔∸మහ࡞⵫✒ࡊ࡙࠷ࡾ࠾ࡼ࡚࠵

ࡾࠊ⌟ᅹ⏕࠷ࡼࡿ࡙࠷ࡾ༈ⷾဗࡵ᳔∸㉫″ ࡚࠵ࡖࡒࡽࠉ⏍⌦Ὡᛮ᳔∸ᠺฦ࠾ࡼㄇᑙࡈ

ࡿࡒࡵࡡ࠿⏕࠷ࡼࡿ࡙࠷ࡾౚ࠿ኣ࠷ࠊྙᠺ༈ⷾဗࡵ᳔∸ࡡ⏍⌦Ὡᛮᠺฦࢅ࣭ࣛࢺ໩ྙ

∸࡛ࡊ࡙㛜Ⓠࡈࡿࡒࡵࡡ࠿ᑛ࡝ࡂ࡝࠷ࠊࡐࢆ࡝⏍⌦Ὡᛮ᳔∸ᠺฦࡡ◂✪ࡢࠉⲙᮇ᳔∸

࠿୯ᚨ࡞⾔ࢂࡿ࡙ࡀ࡙࠽ࡽࠉᮄᮇ᳔∸࡞ᑊࡌࡾ◂✪࠿Ẓ㍉Ⓩᑛ࡝࠷ࡡ࠿⌟≟࡚࠵ࡾࠊ ࡊ࠾ࡊ࡝࠿ࡼࠉྱ᭯ᠺฦࡡ⏍⌦Ὡᛮ∸ ㈻࡛ࡊ࡙ࡡྊ⬗ᛮ࡞ࡢ୦⩽࡚ᇱᮇⓏ࡞ᕣ࠿࡝࠷

࡛⩻࠻ࡼࡿࡾࠊࡐࡆ࡚ࠉᗀᓞ ┬ࢅ୯ᚨ࡛ࡊ࡙᤿㞗ࡊࡒᮄᮇ᳔∸࠾ࡼ⏍⌦Ὡᛮᠺฦࡡฦ 㞫࡛ᵋ㏸◂✪ࢅ⾔ࡖࡒࠊ

ࡐࡡ⤎ᯕࠉ࢜ࣕ

Torreya nuciferaࠉࢾࢫࢤ Thuja standishiiࠉࢦ࣠ࣚ Chamaecyparis

pisifera

ࠉ ࢹࢺ࣏ࢵ

Abies sachalinensis

࠾ࡼࡢࢡ࣑ࣚ㝟ᛮ⣵Ⳟ࡚࠵ࡾ㯜Ⰵࣇࢺࢗ⌣Ⳟࡷᯜ

ⲙⳞ࡞ᑊࡊ࡙ᢘⳞὩᛮࢅ᭯ࡌࡾࢩࢷࣜ࣋ࣤㄇᑙమࡷ㦭᰹ ㌹⛛ࡊࡒࣚࢿࢪࢰࣤㄇᑙమࢅࠉ ࢲࣔ࣍࢝ࣕ

Torreya nucifera var. radicans

࠾ࡼࡢࠉ⹰ṉࡷṉ࿔⑋ࡡཋᅄ࡛⩻࠻ࡼࡿ࡙࠷

ࡾཾ⭅⣵Ⳟ࡞ᑊࡊ࡙ᢘⳞὩᛮࢅᣚࡗࢩࢷࣜ࣋ࣤㄇᑙమࢅฦ㞫ࡊࡒࠊ

Ⓠ࢝ࣤࡡ㸧␊ ┘ࡡࢪࢷࢴࣈ࡚࠵ࡾࣈ࣓࣭ࣞࢨࣘࣤṹ㝭ࢅᢒโࡌࡾ∸㈻ࢅࢪࢠ࣭ࣛࢼ

ࣤࢡࡌࡾࡒࡴ࡞ࠉⓆ࢝ࣤࣈ࣓࣭ࣞࢰ࣭࡞ࡻࡽ࢝ࣤ⣵⬂࡞ᙟ㈻㌹ ᥦࡌࡾ BJ6 ⣵⬂ࢅ⏕࠷

ࡒᢘⓆ࢝ࣤࣈ࣓࣭ࣞࢰ࣭Ὡᛮ モ㥺ࢅᣞᵾ࡛ࡊ࡙Ὡᛮᠺฦࡡฦ㞫ࢅ⾔ࡖࡒ⤎ᯕࠉ࢜ࣛࣤ

Chaenomeles sinensis

࠾ࡼࡢࣛࢡࢻࣤ㒼⢶మཀྵࡦࢹࣛࢷࣜ࣋ࣤㄇᑙమࢅࠉ࢙ࢥࢿ࢞

Styrax japonica

࠽ࡻࡦࢨ࣓ࣞࢩ

Lindera triloba

࠾ࡼࡢࣛࢡࢻࣤㄇᑙమࢅὩᛮᠺฦ࡛ࡊ

࡙ฦ㞫ࡊࡒࠊ

ᙁ࠷ᢘ㓗໩Ὡᛮࢅ♟ࡊࡒࣀࣤࢿ࢞

Alnus japonica

࠾ࡼࡢࣄࣆ࢘ࢼࣜࣉࣈࢰࢿ࢕ࢺ࠿

ᢘ㓗໩∸㈻࡛ࡊ࡙ฦ㞫ࡈࡿࠉฦᏄ୯ࡡ࢜ࢷࢤ࣭ࣜᵋ㏸࠿Ὡᛮ࡞㔔さ ࡚࠵ࡾࡆ࡛࠿᪺ࡼ

࠾࡞࡝ࡖࡒࠊ

PC12 ⣵⬂࡞ᑊࡌࡾ♼⤊✲㉫ఘᘿὩᛮࢅᣞᵾ࡛ࡌࡾࢪࢠ࣭ࣛࢼࣤࢡ࡚ࣃࢿ࢞

Chamaecyparis obtusa

࡞ᙁ࠷Ὡᛮ࠿ヾࡴࡼࡿࠉฦ㞫ࡈࡿࡒࣛࢡࢻࣤㄇᑙమࡡୌ㒂࡞ᙁ࠷

Ὡᛮ࠿ヾࡴࡼࡿࡾ࡛භ࡞ࠉࣛࢡࢻ࡛ࣤࢬࢪ࢞ࢷࣜ࣋ࣤࡡ⦨ྙమ࡞ࡵᙁ࠷Ὡᛮ࠿ヾࡴࡼ

ࡿࡒࠊ

௧୕ࡡ⤎ᯕࠉ⏍⌦Ὡᛮ∸ ㈻᥀⣬ࡡࡒࡴࡡ⣪ᮞ࡛ࡊ࡙ᮄᮇ᳔∸࡞᭿ᙽ࠿ᣚ࡙ࡾࡆ࡛࠿

᪺ࡼ࠾࡞࡝ࡖࡒࠊ

ୌ ⯙ ㅦ ⁿ

ㅦ ⁿ さ ᪠

A 1

Vibrio choleraeࢣࢿ࣑ࡡࣝࢹࣞࣤ㡷ᇡ࡞࠽ࡄࡾኣᵕᛮ࡛᩺ぞmsDNAࡡᵋ㏸よᯊ ې஬୕ ྀ⨶Ꮔ^㸦ࠉ㔕㑒 ⨶Ṅ^㸦ࠉ▴⏛ Ὂ஦㑳^㸦ࠉ⠓⏛ ⣟⏠^㸧ࠉᓞᮇ ᩒ^㸦

㸝^㸦ᗀᓞኬ㝌࣬⏍∸ᅥ࣬㣏ဗ⾠⏍㸡^㸧ᒱᒜ⌦ኬ࣬⌦࣬⮣ᗃ⏍࿤㸞

࠘┘Ⓩ࠙⣵Ⳟࡡ㏣㌹෕㓕⣪ࡢ㸡⣵⬂හ࡚multicopy single-stranded DNA㸝msDNA㸞࡛࿣ࡣࡿࡾ

≁Ṟ࡝ RNA-DNA 々ྙమࡡྙᠺ࡞ᚪ㡪࡚࠵ࡾ࠿㸡ࡐࡡ⏍⌦Ⓩណ⩇ࡢ᪺ࡼ࠾࡞࡝ࡖ࡙࠷࡝࠷ࠊ

msDNAࢅࢤ࣭ࢺࡌࡾ㡷ᇡ㸝msr-msd㸞࡛㏣㌹෕㓕⣪㐿ఎᏄ㸝ret㸞ࡢ㸡ࣝࢹ࡛ࣞࣤ࿣ࡣࡿࡾ࠵ࡾ

⛸ࡡྊິᛮ㐿ఎᅄᏄࢅᵋᠺࡊ࡙࠷ࡾࠊࡆࡿࡱ࡚ࡡ◂✪࡚㸡Vibrio choleraeࡡࣝࢹࣞࣤ-Vc95ࡢ㸡 Ὦ⾔ᛮࢤࣝࣚࡡཋᅄⳞ࡚࠵ࡾ⾉ΰᆵ O1/O139 ᰬ࡞ࡢᏋᅹࡊ㸡⑋ཋᛮࡡ఩࠷ non-O1/non-O139

ᰬ࡞ࡢ⛝࡞ࡊ࠾Ꮛᅹࡊ࡙࠷࡝࠷ࡆ࡛࠿᪺ࡼ࠾࡞࡝ࡖ࡙࠷ࡾࠊࡱࡒ㸡non-O1/non-O139ᰬࡡࣝࢹ

ࣞࣤ㡷ᇡ࡞ࡢ㸡ࣝࢹࣞࣤࡡ௥ࢂࡽ࡞Ⳟᰬࡇ࡛࡞␏࡝ࡾ㛏ࡈࡡDNA᩷∞࠿᣼ථࡈࡿ࡙࠽ࡽ㸡ኣ

ࡂࡢ⣑100 bpࡡ⧖ࡽ㏁ࡊ㒼าࢅᣚࡗࡆ࡛࠿᪺ࡼ࠾࡞࡝ࡖ࡙࠷ࡾࠊࡆࡡࡆ࡛࠾ࡼ㸡V. choleareࡡ

ࣝࢹࣞࣤ㡷ᇡࡢ⤄ᥦ࠻ࡡ࣌ࢴࢹࢪ࣎ࢴࢹ࡚࠵ࡾྊ⬗ᛮ࠿♟ြࡈࡿ࡙࠷ࡾࠊᮇ◂✪࡚ࡢ㸡V.

choleraeࢣࢿ࣑࡞࠽࠷࡙ኣᵕᛮࡡぜࡼࡿࡾࣝࢹࣞࣤ㡷ᇡ࡞╌┘ࡊ㸡◂✪ࢅ⾔ࡖࡒࠊ

࠘⤎ᯕ࡛⩻ᐳ࠙non-O1/non-O139ᰬࡡࣝࢹࣞࣤ㡷ᇡࡡሲᇱ㒼าࢅỬᏽࡊ㸡O1/O139ᰬ࡛Ẓ㍉ࡌ

ࡾࡆ࡛࡚ࣝࢹࣞࣤࡡࢣࢿ࣑୕ࡡ᣼ථన⨠ࢅ᪺ࡼ࠾࡞ࡊࡒࠊࡈࡼ࡞㸡non-O1/non-O139ᰬࡡ࠹ࡔ 1ᰬ࠾ࡼ᩺ぞࡡmsDNA࡛ࣝࢹࣞࣤࢅⓆぜࡊ㸡Maxam-GilbertἪ࡞ࡻࡖ࡙msDNAࡡᵋ㏸ࢅ᪺ࡼ

࠾࡞ࡊࡒࠊࡱࡒ㸡ࣝࢹࣞࣤࡡሲᇱ㒼าよᯊࡡ⤎ᯕ㸡᩺ぞࡡࣝࢹࣞࣤࡢ㸡Salmonellaࡡࣝࢹࣞࣤ

-St85 ࡛㐿ఎᏄᵋᠺࡷሲᇱ㒼าࡵ㢦జࡊ࡙࠷ࡾࡆ࡛࠿᪺ࡼ࠾࡞࡝ࡖࡒࠊࡐࡊ࡙㸡᩺ࡒ࡝ࣝࢹࣞ

ࣤࡵࡆࡿࡱ࡚࡞▩ࡼࡿ࡙࠷ࡾࣝࢹࣞࣤ-Vc95࡛ྜྷࡋ㡷ᇡ࡞᣼ථࡈࡿ࡙࠷ࡾࡆ࡛࠿ࢂ࠾ࡖࡒࠊ௧

୕ࡡ⤎ᯕࡢ㸡ࣝࢹࣞࣤ㡷ᇡ࠿V. choleraeࢣࢿ࣑ࡡኣᵕᛮ࡞ᐞ୙ࡊ࡙࠷ࡾࡆ࡛ࢅ♟ြࡊ࡙࠷ࡾࠊ

A 2

ይ⇍Ⳟ⏜ᮮࣄ࢛ࢲࣤ⏍ྙᠺ⣌㓕⣪࡞㛭ࡌࡾ◂✪–Thermus thermophilus⏜ᮮKAPA ྙᠺ

㓕⣪ࡡ㐿ఎᏄ᥀⣬࡛㓕⣪Ὡᛮ᳠ฝ̽

ې❉⏛ 㧏⚽ࠉୖ㔕 ᝙ᖲࠉ࿰ἠ ይゝ 㸝㫵ཱིኬᕝࠉ⏍ᚺᕝ㸞

࠘┘Ⓩ࠙ࣄ࢛ࢲࣤࡢ྘⛸࢜ࣜ࣍࢞ࢨ࣭ࣚࢭࡡ⿭㓕⣪࡛ࡊ࡙᚜⏍∸ࡓࡄ࡚࡝ࡂ㧏➴⏍∸࡞࡛ࡖ࡙

ࡵᚪ㡪ࡡ⏍మහ∸㈻࡚࠵ࡾࠊᠻࠍࡢ᭩㎾ࠉ௑ࡱ࡚ධࡂሒ࿈ࡡ↋࠷ይ⇍Ⳟࡡࣄ࢛ࢲࣤ⏍ྙᠺ㓕⣪

ࢅぜฝࡊࡒࠊ௑ᅂࠉ㧏ᗐይ⇍Ⳟ T. thermophilus HB8 ᰬࡡࢣࢿ࣑ࢸ࣭ࢰ࣭࣊ࢪ࡚ࡢ Glycine acetyltransferase (GlyAcT) ࡛࢓ࢿࢷ࣭ࢨࣘࣤࡈࡿ࡙࠷ࡾTTHA1582࠿ࣄ࢛ࢲࣤ⏍ྙᠺ⣌㓕⣪ࡡ KAPAྙᠺ㓕⣪(KAPA synthase)Ὡᛮࢅ᭯ࡊ࡙࠷ࡾࡆ࡛ࢅぜฝࡊࡒࡡ࡚ሒ࿈ࡌࡾࠊ

࠘᪁Ἢ࡛⤎ᯕ࠙T. thermophilus HB8ࡡࢣࢿ࣑࡞ࢤ࣭ࢺࡈࡿ࡙࠷ࡾTTHA1582ࡢࣁ࢕࢛࢕ࣤࣆ࢚

࣏ࢷ࢔ࢠࢪ࡞࠽࠷࡙GlyAcT (EC: 2.3.1.29)࡛࢓ࢿࢷ࣭ࢨࣘࣤࡈࡿ࡙࠷ࡾ࠿ࠉ ᠻࠍࡢࣄ࢛ࢲࣤ

⏍ྙᠺ⣌㓕⣪ࡡKAPA synthase (EC: 2.3.1.47)࡞ᑊࡊ࡙ࡵ㧏࠷┞ྜྷᛮ⣑50%ࢅ♟ࡌࡆ࡛ࢅ

ぜฝࡊࡒࠊࡐࡆ࡚ࡆࡿࢅE. coliහ࡚⤄ᥦ࠻ࢰࣤࣂࢠ㈻࡛ࡊ࡙㧏Ⓠ⌟ࡈࡎ⢥⿿ࡊࠉ≁ᛮよ᪺ࢅモ

ࡲࡒࠊࡐࡡ⤎ᯕࠉᮇ㓕⣪ࡢGlyAcTὩᛮࡓࡄ࡚࡝ࡂࠉKAPA synthaseὩᛮࡵ᳠ฝ࡚ࡀࠉ୦Ὡᛮ

ࢅ♟ࡌิࡴ࡙ࡡౚ࡚࠵ࡖࡒࠊࡐࡡ⮫㐲Ὼᗐ࠽ࡻࡦ⇍Ꮽᏽᛮࡢ᪜▩㓕⣪࡛Ẓ࡬࡙᭩ࡵ㧏࠷ೋࢅ♟

ࡊࡒࠊ㻃 㻃

15 

A 3

᚜⏍∸࡞࠽ࡄࡾࢬࣝࣤ㓗㑇ඔ࡞㛭୙ࡌࡾ㐿ఎᏄࡡ᥀⣬࡛よᯊ

㛓⬝ ⏜ᕵᏄࠉ℀⏛ ᑠ྿ࠉې㜨ཾ ฺᩝ㸝┬❟ᗀᓞኬ࣬⎌ሾ⛁Ꮥ㸞

࠘┘Ⓩ࠙㎾ᖳࠉ⏐ᴏὩິࡻࡽᤴฝࡈࡿࡾ㔔㔘ᒌࡷ㔘ᒌᵕඔ⣪ࡡỗ᯹ࠉᅂ཭࠿ၡ㢗࡛࡝ࡖ࡙࠷ࡾࠊ ୯࡚ࡵ⎌ሾ୯࡞ᨲฝࡈࡿࡒࢬࣝࣤࡡᅂ཭࠿᭻ࡱࡿ࡙࠽ࡽࠉ᚜⏍∸ࡡ㓗໩㑇ඔཬᚺ࡞ࡻࡾࢬࣝࣤ

ᅂ཭Ἢ࠿Ἰ┘ࡈࡿ࡙࠷ࡾࠊࡊ࠾ࡊࠉ᚜⏍∸ࡡࢬࣝࣤ㓗࡞ᑊࡌࡾ㓗໩㑇ඔᶭᵋ࡞ࡗ࠷࡙ࡢレ⣵࡝

よ᪺࠿࡝ࡈࡿ࡙࠷࡝࠷ࠊࡐࡆ࡚ࠉᮇ◂✪࡚ࡢ᚜⏍∸ࡡࢬࣝࣤ㓗ࡡ௥ㅨ࡞㛭ࡌࡾ㐿ఎᏄࢅ᩺ぞ࡞

༟㞫ࡊࡒࢬࣝࣤ㓗㑇ඔⳞ࠾ࡼ᥀⣬ࡊࠉࢬࣝࣤ㓗࡞ᑊࡌࡾࣈ࣓࣭ࣞࢰ࣭ᛮ㐿ఎᏄࠉ㓗໩㑇ඔ࡞㛭

୙ࡌࡾ㐿ఎᏄࡡࢠ࣭ࣞࢼࣤࢡࠉよᯊࢅ⾔ࡖࡒࠊ

࠘᪁Ἢࠉཀྵࡦ⤎ᯕ࠙ࡱࡍࠉࢬࣝࣤ㓗࡞ᑊࡊ࡙ᚺ➽⬗ࢅ♟ࡌ㐿ఎᏄࡡ᥀⣬ࢅ⾔࠹ࡒࡴࠉࢬࣝࣤ㓗 㑇ඔⳞᰬࡡࢣࢿ࣑DNAࢅࣚࣤࢱ࣑ࢠ࣭ࣞࢼࣤࢡἪ࡞ࡻࡖ࡙࣭ࣝ࣎ࢰ࣭࣊ࢠࢰ࣭࡞⤄㎲ࡲࠉࢬ

ࣝࣤ㓗ࡡ᭯↋࡚Ⓠකࡵࡊࡂࡢ࿆ⰅཬᚺࢅẒ㍉ࡌࡾࡆ࡛࡚ࠉࢬࣝࣤ㓗ࡡ᭯↋࡚ን໩ࡡ࠵ࡾኬ⭘Ⳟ

ᰬ(SeP-5)ࢅ⋋ᚋࡊࡒࠊࡆࡡᰬ࠾ࡼ㐿ఎᏄ᩷∞ࡡよᯊࢅ⾔ࡖࡒ࡛ࡆࢀࠉ⣑6.0kbpࡡ᣼ථ᩷∞࠿

☔ヾࡈࡿࠉᑛ࡝ࡂ࡛ࡵ ࡗࡡ࢛࣭ࣈ࣭ࣤࣛࢸ࢔ࣤࢡࣆ࣭࣑ࣝ(ORF)࠿Ꮛᅹࡊࡒࠊࡐࡡ࠹ࡔࡥ࡛

ࡗࡡ47+ ୕Ὦᇡ࡞ࣈ࣓࣭ࣞࢰ࣭㒼า࡛ᛦࢂࡿࡾ㡷ᇡ࠿☔ヾࡈࡿࡒࠊࡱࡒࠉORF ࡚ࡢࠉ࠷ࡂࡗ

࠾ࡡࢬࣝࣤ㓗㑇ඔⳞ࡞࠽࠷࡙ࢬࣝࣤ㓗㑇ඔ࡞㛭୙࠿♟ြࡈࡿ࡙࠷ࡾ࣓ࣛࣇࢸࣤ㓕⣪࡛㧏࠷┞

ྜྷᛮ࠿☔ヾ࡚ࡀࡒࠊ

A 4

Acidithiobacillus ferrooxidansࡡ୹さ࡝࣭࣎ࣛࣤࢅࢤ࣭ࢺࡌࡾ㐿ఎᏄRPSࡡⓆ⌟࡞ཀྵ࡯ࡌ⎌

ሾᅄᏄࡡᙫ㡢

ې࣓ࣀࢴ࣒ࢺ ࣏ࣤࢨࣖࣜࠉ㔘ᑹ ᚽⰶࠉᮙᑹ ๙ࠉ୕ᮟ ୌ㞕㸝ᒱᒜኬ㝌࣬⮤↓࣬ࣁ࢕࢛㸞

㕪㓗໩⣵ⳞAcidithiobacillus ferrooxidansࡢ㸡ይ㓗ᛮࡡ໩Ꮥྙᠺ≺❟ᰜ㣬⣵Ⳟ࡚࠵ࡾࠊ㓗ᛮ⎌ሾ

࡞ࡈࡼࡈࡿ࡙࠷ࡾአ⭯ࡢ㸡⎌ሾን໩࡫ࡡᚺ➽࡞࠽࠷࡙㔔さ࡚࠵ࡾ࡛⩻࠻ࡼࡿࡾࠊᮇⳞࡡአ⭯ࡡ

୹さ࡝࣭࡛࣎ࣛࣤࡊ࡙ omp40 ࢰࣤࣂࢠ㈻࠿ྜྷᏽࡈࡿ࡙࠷ࡾࠊࡆࡡ࣭࣎ࣛࣤࡢ㸡ㇿ࡞ᖈ㞹ࡊࡒ

࢕࢛ࣤࢲࣔࣤࢾࣜࢅᙟᠺࡊ㸡ࣛࣤ㓗㣒㣱᮪௲ୖࡡ⣵⬂හ࡚ࡐࡡྱ㔖࠿ቌຊࡌࡾࡆ࡛࠿ሒ࿈ࡈࡿ

࡙࠷ࡾ࠿㸡ࣛࣤ㓗㣒㣱௧አࡡአ㒂⎌ሾࡡን໩࠿㸡omp40ࡡⓆ⌟࡞ཀྵ࡯ࡌᙫ㡢࡞ࡗ࠷࡙ࡢ᳠ゞࡈ

ࡿ࡙࠷࡝࠷ࠊ

omp40㐿ఎᏄࡡୌ㒂ࢅࣈ࣭ࣞࣇ࡞⏕࠷࡙㸡ሲ⃨ᗐ㸡ࣛࣤ㓗㣒㣱㸡ᇰᆀࡡ S-ን໩࠿RPSࡡ

Ⓠ⌟࡞ཀྵ࡯ࡌᙫ㡢ࢅࢿ࣭ࢧࣤよᯊ࡞ࡻࡖ࡙᳠ゞࡊࡒࠊomp40ࡡⓆ⌟ࡡὩᛮ໩ࡢ㸡ࣛࣤ㓗㣒㣱⣵

⬂௧አ࡞3F(O Ꮛᅹୖ࡚ᇰ㣬ࡊࡒ⣵⬂࡚ࡵふᐳࡈࡿࡒࠊKClࡢⓆ⌟ࢅὩᛮ໩ࡊࡒ࠿Na2SO4ࡢᙫ 㡢ࢅཀྵ࡯ࡈ࡝࠷ࡆ࡛࠾ࡼ㸡NaCl ࡞ࡻࡾⓆ⌟ࡡὩᛮ໩ࡢ Cl^-࡞ࡻࡾࡵࡡ࡚࠵ࡾ࡛᥆ῼࡈࡿࡒࠊ

ࡱࡒ㸡ᇰᆀࡡpH࠿2.5ࡻࡽ࢓ࣜ࢜ࣛഁ࡞࡝ࡾ࡛ࡐࡡⓆ⌟࠿Ὡᛮ໩ࡈࡿࡾࡆ࡛࠿᪺ࡼ࠾࡛࡝ࡖ ࡒࠊ

A 5 Functional Role of Domain III of Cry Insecticidal Proteins from Bacillus thuringiensis ې Mohammad Tofazzal Hossain Howlader, Yumiko Ishida, Akiko Nakaguchi, Keiko Oka, Kouji Ohbayashi, Masashi Yamagiwa, Tohru Hayakawa, and Hiroshi Sakai

(Graduate School of Natural Science and Technology, Okayama University)

Cry1C, one of lepidopteran-specific crystal proteins from

Bacillus thuringiensis exhibits

potent cytotoxicity against Sf9, an insect cell line from

Spodoptera frugiperda. Cry1Aa and

Cry4A which are lepidopteran- and dipteran-specific crystal proteins, respectively, exhibit no cytotoxicity towards Sf9 cells. When the domain III of Cry1C was replaced by the domain III of Cry1Aa or Cry4A, the resulting hybrid Cry1C proteins maintained the cytotoxicity to Sf9 cells, indicating that domain III of Cry1C can be functionally replaced from other Cry proteins which are closely or distantly related to Cry1C. It also suggested that domain III was not concerned with the specificity in recognizing Sf9 cells. Interestingly, insertion of a dipeptide Pro-Gly in between the domain II and domain III rendered cytotoxicity of the resulting Cry1C hybrid proteins markedly reduced or lost. This implied that domain III was involved in maintaining a functional structure of Cry1C.

A 6

Shewanella violaceaࢨࢹࢠ࣑ࣞc₅࡞࠽ࡄࡾࢩࢪࣜࣆ࢔ࢺ⤎ྙࡡᙲ๪

ې➁୯ ⪯ࠉ୔ᮇᮄ ⮫Ᏻ㸝ᗀኬ㝌⏍∸ᅥ㸞

࠘┘Ⓩ࣬⫴ᬊ࠙ ᮇ◂✪ࡡ┘Ⓩࡢ㸡⾪㢗Ⳟࢨࢹࢠ࣑ࣞc5㸝SVcytc5㸞࡞ᏋᅹࡌࡾSS⤎ྙࡡᙲ๪

ࢅ᪺ࡼ࠾࡞ࡌࡾࡆ࡛࡚࠵ࡾࠊSVcytc₅࡚ࡢ㸡Cys-59࡛Cys-62࠿ฦᏄහ࡚SS⤎ྙࢅᙟᠺࡊ࡙࠷

ࡾࠊ┞ྜྷ࡝ࢨࢹࢠ࣑ࣞ㹘ࡢ㸡௙ࡡShewanellaᒌࡷAzotobacterᒌ࡞ࡵぜࡼࡿ㸡ྜྷᵕ࡞SS⤎ྙࢅ

ᣚࡖ࡙࠷ࡾࠊࡊ࠾ࡊ㸡ࡆࡡSS⤎ྙ࠿⺦Ⓣ㈻ࡡᏭᏽᛮࡷᶭ⬗࡞࡜ࡡࡻ࠹࡞ᙫ㡢ࡌࡾࡡ࠾ࡢᮅ▩

࡚࠵ࡾࠊ

࠘᪁Ἢ࣬⤎ᯕ࠙ SVcytc₅ࡡCys-59࡛Cys-62ࢅࡐࡿࡑࡿAla࡞⨠ᥦࡊࡒC59A/C62Aን␏మࢅ

ኬ⭘Ⳟ࡚Ⓠ⌟ࡈࡎࡒࠊን␏మࡡྊっྺ཭㸡ළ஦Ⰵᛮ㸡࠽ࡻࡦᖏ☚ᛮNMRࢪ࣋ࢠࢹࣜࡢ㸡㔕⏍

మ࡛ୌ⮬ࡊࡒࠊࡈࡼ࡞㸡ን␏మࡡ㓗໩㑇ඔ㞹నࡵ㔕⏍మ࡛ୌ⮬ࡊࡒࡆ࡛࠾ࡼ㸡SS ⤎ྙ࠿㝎ཡ ࡈࡿ࡙ࡵ㸡ࣉ࣑࿔㎰ࡡᵋ㏸ࡷᶭ⬗࡞ᙫ㡢ࡊ࡝࠷ࡆ࡛࠿᪺ࡼ࠾࡞࡝ࡖࡒࠊࡊ࠾ࡊ㸡♟ᕣ㉦ᰕᆵ⇍

㔖ゝࢅ⏕࠷࡙⇍Ꮽᏽᛮࢅῼᏽࡊࡒ࡛ࡆࢀ㸡ን␏మࡢ㔕⏍మ࡞Ẓ࡬࡙ንᛮῺᗐ࠿ 24Υୖ࠿ࡾ࡝

࡜㸡Ꮽᏽᛮ࠿఩ୖࡊࡒࠊࡐࡡཋᅄࡢ࢙ࣤࢰࣜࣅ࣭࠿΅ᑛࡊࡒ࠾ࡼ࡚࠵ࡽ㸡SS ⤎ྙࢅᙟᠺࡌࡾ

Cysࡡ◪㯜ཋᏄࡡ㝎ཡ࡞ࡻࡽ㸡࿔㎰ࡡ࢓࣐ࢿ㓗ഁ㙈ࡡ⤎ྙᩐ࠿΅ᑛࡊ࡙࠷ࡾࡆ࡛࠿᪺ࡼ࠾࡞࡝

ࡖࡒࠊ

17 

A 7

ฦ⿛㓕ẍࡡ々ᩐ⭯㈇㏳ࢰࣤࣂࢠ㈻ᑻᅹ࡞࠽ࡄࡾࢪࢷ࣭ࣞࣜࡡ㔔さᛮ

ᒷᇖ ▩Ꮔࠉې➁ᕖ ⷽ㸝㤮ᕖኬ࣬㎨࣬ᚺ⏕⏍∸㸞

ࢪࢷ࣭ࣞࣜࡢ┷ᰶ⏍∸࡞ᗀࡂಕᏋࡈࡿࡒ⭯ࡡᵋᠺᠺฦࡡ㸦ࡗ࡚ࠉ⣵⬂⭯୕ࡡࣚࣆࢹ࡛࿣ࡣࡿ

ࡾ༇⏤࡞㧏ᐠᗐ࡞Ꮛᅹࡊ࡙࠷ࡾࠊ࢙ࣜࢥࢪࢷ࣭ࣞࣜࡷࢪࣆ࢔ࣤࢥ⢶⬙㈻࡝࡜࠿㧏ᐠᗐ࡚ᑻᅹࡌ

ࡾࣚࣆࢹ࠿᭩㎾࡞࡝ࡖ࡙ฝⰾ㓕ẍ࡞ࡵᏋᅹࡌࡾࡆ࡛࠿ሒ࿈ࡈࡿࠉ⭯ࢰࣤࣂࢠ㈻ࢅ⤄ࡲ㎲ࢆ࡚࢛

ࣜ࢝ࢾࣚ㛣ࡡ㍲㏞ࡷࢨࢡࢻࣜఎ㐡࡞㛭୙ࡊ࡙࠷ࡾྊ⬗ᛮ࠿ሒ࿈ࡈࡿ࡙࠷ࡾࠊୌ᪁ࠉฦ⿛㓕ẍࡵ

୹さ࡝ࢪࢷ࣭ࣞࣜࡢ࢙ࣜࢥࢪࢷ࣭࡚ࣞࣜ࠵ࡾࡆ࡛࠿ࢂ࠾ࡖ࡙࠷ࡾ࠿ࠉࡐࡡ⏍ྙᠺᶭᵋࡷ⏍⌦Ⓩ

ᙲ๪࡞ࡗ࠷࡙ࡢ࡮࡛ࢆ࡜▩ࡼࡿ࡙࠷࡝࠷ࠊࡐࡆ࡚ฦ⿛㓕ẍࢣࢿ࣑ࢸ࣭ࢰ࣭࣊ࢪ࠾ࡼฝⰾ㓕ẍࡡ

࢙ࣜࢥࢪࢷ࣭ࣞࣜྙᠺ⣌㐿ఎᏄERG㐿ఎᏄ࡛┞ྜྷᛮࡡ㧏࠷㐿ఎᏄࢅྜྷᏽࡊࠉ㐿ఎᏄ◒ቪᰬࢅ

ཱིᚋࡊ࡙よᯊࢅ⾔ࡖࡒࠊࡐࡡ⤎ᯕࠉฦ⿛㓕ẍerg6erg2࡝࡜ࡡ㐿ఎᏄ◒ቪᰬࡢ☔࠾࡞࢙ࣜࢥࢪ ࢷ࣭ࣞࣜ࠿᳠ฝࡈࡿ࡝࠷ࡆ࡛ࢅ☔ヾࡊࡒࠊฦ⿛㓕ẍerg6 ◒ቪᰬࡢࢨࢠࣞ࡫࢞ࢨ࣐ࢺࡷࢪࢰࢗ

ࣞࢪ࣎ࣛࣤ࡝࡜ࡡⷾ๠࡞ࡢវུᛮࢅ♟ࡌ࠿ࠉ࢓ࣤ࣌ࢷࣛࢨࣤ'ࡷࢻ࢕ࢪࢰࢲࣤ࡝࡜ࡡ࢙࣎ࣛࣤ

⣌ᢘ⏍∸㈻࡞ࡢ㧏࠷⪇ᛮࢅ♟ࡊࡒࠊฦ⿛㓕ẍ erg6 ◒ቪᰬࡢᾦ⬂ࢰࣤࣂࢠ㈻㍲㏞ࡷ࢙ࣤࢺࢦ࢕

ࢹ࣭ࢨࢪ࡝࡜࡞ࡢ␏ᖏࢅ♟ࡈ࡝࠷࠿ࠉ⣵⬂⭯ᑻᅹATPase࡚࠵ࡾPma1ࢰࣤࣂࢠࡷ࢓࣐ࢿ㓗ࢹ

ࣚࣤࢪ࣭࣎ࢰ࣭࡝࡜ࡡ⣵⬂⭯࡫ࡡᑻᅹ࠿㔕⏍ᰬ࡛ࡢ␏࡝ࡾࡆ࡛࠿᪺ࡼ࠾࡞࡝ࡖࡒࠊࡆࡡ⤎ᯕ࠾

ࡼฦ⿛㓕ẍ⣵⬂⭯ࢰࣤࣂࢠ㈻ࡵࡐࡡᑻᅹ࡞ࡢ࢙ࣜࢥࢪࢷ࣭ࣞࣜ࠿㔔さ࡝ᙲ๪ࢅᯕࡒࡊ࡙࠷ࡾ

ࡆ࡛࠿♟ြࡈࡿࡒࠊ

A 8

^{ฝⰾ㓕ẍ࡞࠽ࡄࡾ}CEN5-HIS3㛣ࡡ㒂న≁␏Ⓩ⤄ᥦ࠻ࡡຝ⋙୕᪴ን␏ᰬࡡよᯊ

ېᰏᮇ ᨼᙪࠉᮿᓧ ᾀ᪺ࠉ⛑㔕 ⌮஄㸝⚗ᒜኬ࣬⏍࿤ᕝ࣬⏍∸ᕝ㸞

ᠻࠍࡢࠉ᯹Ⰵమࡡᰶහ཭⣙࣒࢜ࢼࢫ࣑ࢅࠉS. cerevisiaeࡡCEN5-HIS3㛣ࡡ⤄ᥦ࠻ຝ⋙࠿୕᪴

ࡊࡒHCH6ን␏ᰬࢅ⏕࠷࡙よ᪺ࡊࡻ࠹࡛ࡊ࡙࠷ࡾࠊHCH6ን␏ᰬࡢࠉῺᗐវུᛮ࡚ࠉ᯹Ⰵమࡡ

ᰶහ㒼⨠࡛cell integrity࡞␏ᖏࢅ♟ࡊࠉᰶහ㒼⨠࡛⣵⬂㈻හ㒼⨠ࡡ㛣࡞ࢠࣞࢪࢹ࣭ࢠ࠿Ꮛᅹࡌ

ࡾྊ⬗ᛮ࠿♟ြࡈࡿࡒࠊࡱࡍࠉHCH6ᰬࡡን␏ࡡཋᅄ㐿ఎᏄࢅよ᪺ࡌࡾࡒࡴ࡞cell integrity⤊

㊪഼࡚ࡂ㐿ఎᏄࡡ◒ቪᰬࡡῺᗐវུᛮࢅㄢ࡬ࡒࠊࡐࡡ⤎ᯕࠉmpk1Δᰬࠉssd1Δᰬࠉ࠽ࡻࡦbck1Δ

ᰬࡡ ᰬ࠿Ὼᗐវུᛮࢅ♟ࡊࡒࠊࡆࡿࡼ ᰬࡡ࠹ࡔࠉssd1Δᰬࡡࡲ࠿HCH6ን␏ᰬ࡛ஹ㞟ࡊ࡙

ࡵῺᗐវུᛮࢅᅂᚗࡊ࡝࠾ࡖࡒࠊࡱࡒࠉHCH6ን␏ᰬ࡞㔕⏍ᆵSSD1㐿ఎᏄࢅᑙථࡌࡾࡆ࡛࡚

Ὼᗐវུᛮ࠿ᅂᚗࡊࡒࠊࡆࡿࡼࡡ⤎ᯕ࠾ࡼࠉSSD1࠿HCH6ᰬࡡን␏ࡡཋᅄ㐿ఎᏄ࡚࠵ࡾ࡛⩻

࠻ࡼࡿࡒࠊࡈࡼ࡞ࠉ⤄ᥦ࠻ຝ⋙ࡢࠉ-(- ን␏ᰬ࡞SSD1ࢅᑙථࡊࡒᰬ࡚ࡢ఩ୖࡊࠉࡱࡒࠉ㔕⏍

ᰬ࡚SSD1 ࢅ◒ቪࡊࡒᰬ࡚ࡢ୕᪴ࡊࡒࡆ࡛࠾ࡼࠉSSD1 ࠿ᰶහ㒼⨠࡞ࡵ㛭୙ࡊ࡙࠷ࡾࡆ࡛࠿♟

ြࡈࡿࡒࠊ௑ᚃࠉHCH6ን␏ᰬ࡛ssd1Δᰬ࡞࠽ࡄࡾᰶහᵋ㏸మࡡ㒼⨠࠾ࡼSSD1ࡡ᯹Ⰵమᰶහ 㒼⨠࡫ࡡ㛭୙ࢅ᪺ࡼ࠾࡞ࡊࡒ࠷ࠊ

A 9

᪝ᮇᾇ῕ᾇ⎌ሾ࠾ࡼࡡ᭯⏕᚜⏍∸ࡡฦ㞫

⚗ᮇ ೸ᾀࠉې௯ᏺ᰷ ⷽ㸝㎾␝ኬᕝ࣬⏍໩ᕝ㸞

㐛ཡ ᖳ㛣࡮࡜ࡡ₧Ề⯪ࡡୠ⏲Ⓩ࡝㛜Ⓠ࡞ࡻࡽࠉ῕ᾇ⏜ᮮࡡᵕࠍ࡝ࢦࣤࣈࣜࡡථᡥ࠿ᐖ᪾

࡞࡝ࡽࠉࡐࡿࡼࢦࣤࣈࣜ࠾ࡼᵕࠍ࡝᚜⏍∸ࡡฦ㞫࠿モࡲࡼࡿ࡙࠷ࡾࠊᮇ◂✪࡚ࡢࠉ᪝ᮇᾇዚᑶ ᾇᕃࡡỀ῕⣑3500m࠾ࡼࡊࢆ࠾࠷6500࡞ࡻࡖ࡙᤿ཱིࡈࡿࡒ῕ᾇᗇἶࢅ⏕࠷ࠉᖼࡗ࠾ࡡ᭯⏕᚜

⏍∸ࡡฦ㞫ࢅモࡲࡒࠊ

ᮇ῕ᾇᗇἶࡻࡽࠉࣈࣞࢷ࢓࣭ࢭ⏍⏐Ⳟࠉ࢓࣐࣭ࣚࢭ⏍⏐Ⳟࠉࡱࡒࡐࡿࡼ୦᪁ࡡ≁ᚡࢅ᭯ࡌࡾ

᚜⏍∸ࠉࡈࡼ࡞Paenibacillusᒌ⣵Ⳟ✽⣪ᅖᏽⳞ᚜⏍∸ࢅ༟㞫ࡊࠉࡐࡿࡼᛮ㈻࡞ࡗ࠷࡙ᐁ㥺ࢅ

⾔ࡖࡒࠊ༟㞫ࡈࡿࡒ᚜⏍∸ࡡ┞ᑊⓏ㓕⣪ὩᛮΥࠉΥࠉΥࠉΥࠉΥ➴ࠉ㢟᚜㙶ふᐳࠉ ୠ௥᫤㛣ࡡῼᏽΥࠉΥࠉΥࠉΥࠉΥࠉΥࠉΥࢅ⾔࠷ࠉࡆࡿࡼ᚜⏍∸ࢅ఩Ὼ⣵Ⳟ 㸝ይ෫Ⳟࠉ⪇෫Ⳟ㸞ࡷ୯Ὼ⣵Ⳟ࡫ࡡฦ㢦ࢅモࡲࡒࠊ⌟ᅹ 8U73&㐿ఎᏄࢅᣞᵾ࡛ࡊࡒ᚜⏍∸ࡡ 㐿ఎᏄよᯊࡵ⾔࠷ࠉᚋࡼࡿࡒ㐿ఎᏄ᝗ሒ࡞ᇱࡘ࠷ࡒ⣌⤣ᶖࢅషᠺࡊࠉฦ㞫Ⳟᰬࡡ⣌⤣㛭౿࡞ࡗ

࠷࡙よᯊ୯࡚࠵ࡽࠉࡆࡿࡼ⤎ᯕ࡞ࡗ࠷࡙ࡵెࡎ࡙ሒ࿈ࡌࡾࠊ

A 10

^{㧏ᗐይሲཿ⣵Ⳟ}Haloarcula japonica RNA࣒࣭࣎ࣛࣚࢭධࢦࣇࣗࢼࢴࢹࡡኬ㔖Ⓠ⌟ཀྵࡦࢦ

ࣇࣗࢼࢴࢹ㛣┞பష⏕ࡡよᯊ

ېᮿ࿝ ᘧஒࠉ⚗ᮇ ᐰ᪺ࠉ௯ᏺ᰷ ⷽࠉ㸝㎾␝ኬᕝ࣬⏍໩㸞

㧏ᗐይሲཿ⣵ⳞHaloarcula japonica TR-1ᰬࡢࠉ⏍⫩ࡡ⮫㐲ሲ⃨ᗐ࠿20㸚ࡡHaloarculaᒌ࡞

ฦ㢦ࡈࡿࡾཿ⣵Ⳟ࡚࠵ࡾࠊୌ⯙Ⓩ࡞ይሲ᚜⏍∸ࡡᣚࡗ㐿ఎᏄࡢࠉ㧏⃨ᗐࡡሲᏋᅹୖ࡚โᚒࡈࡿ

ࡾ㐿ఎᏄ⩄࠿♟ြࡈࡿ࡙࠷ࡾࡵࡡࡡࠉࡐࡡレ⣵࡝◂✪ౚࡢᑛ࡝࠷≟Ἓ࡞࠵ࡾࠊࡆࡡࡻ࠹࡝⫴ᬊ

࠾ࡼࠉይሲ᚜⏍∸ࡡ㐿ఎᏄⓆ⌟ᶭᵋࡡよ᪺ࠉࡱࡒ㐿ఎᏄⓆ⌟ࢨࢪࢷ࣑ࡡᚺ⏕࡛ࡊ࡙⪇ሲᛮ࣬ይ ሲᛮ㓕⣪ࡡ⏍⏐ࢅ┘ᣞࡊࠉࡐࡡࢪࢰ࣭ࢹ࡛ࡊ࡙RNA࣒࣭࣎ࣛࣚࢭࡡධࢦࣇࣗࢼࢴࢹ㐿ఎᏄࡡ ࢠ࣭ࣞࢼࣤࢡࢅ㛜ጙࡊࡒࠊ

ᮇⳞᰬࡡࢦࣇࣗࢼࢴࢹᩐࡢࠉ┷ᰶ⏍∸࡛ྜྷ⛤ᗐ࡚ࠉ10⛸௧୕ࡡࢦࣇࣗࢼࢴࢹ㐿ఎᏄ࡞ࡻࡽ

ᵋᠺࡈࡿ࡙࠷ࡾࡆ࡛࠿᪺ࡼ࠾࡛࡝ࡽᮇ◂✪࡚ࡢࠉࡱࡍᮇⳞᰬRNA࣒࣭࣎ࣛࣚࢭモ㥺⟮හ්ᵋ ᠺࢅ┘Ⓩ࡛ࡊࠉධࢦࣇࣗࢼࢴࢹ㐿ఎᏄࡡ㧏Ⓠ⌟࣊ࢠࢰ࣭ࡡᵋ⠇ࢅ⾔࠷ࠉኬ⭘Ⳟ࡞࠽ࡄࡾ㧏Ⓠ⌟

ᐁ㥺ࢅ⾔ࡖࡒࠊRNA࣒࣭࣎ࣛࣚࢭࢦࣇࣗࢼࢴࢹ㧏Ⓠ⌟࣊ࢠࢰ࣭ࡡᵋ⠇࡞࠽࠷࡙ࡢࠉ-LVWFJ

⢥⿿࠿ྊ⬗࡚ࠉࢰࣤࣂࢠ㈻Ⓠ⌟࡞ࢤ࣭ࣜࢺࢨࣘࢴࢠࢅฺ⏕ࡌࡾpCold࣊ࢠࢰ࣭ࢅ⏕࠷ࠉ┘Ⓩࢰ

ࣤࣂࢠ㈻ࡡ㧏Ⓠ⌟ᐁ㥺ࢅ⾔࠷ࠉNi²⁺࣑࢜ࣚ࡞ࡻࡽ⢥⿿ࢅ⾔ࡖࡒࠊRNA࣒࣭࣎ࣛࣚࢭࡡモ㥺⟮හ

්ᵋᠺࡡࡒࡴࡡ᝗ሒ࡛ࡌ࡬ࡂࠉኬ⭘Ⳟࢅ⏕࠷ࡒTwo-hybridἪ࡞ࡻࡽࠉࢦࣇࣗࢼࢴࢹ㛣┞பష

⏕ࢅよᯊࡊࡒࠊᮇኬఌ࡚ࡢࠉࡐࡡ┞பష⏕࡞ࡗ࠷࡙ࡵెࡎ࡙ሒ࿈ࡌࡾࠊ

19 

B 1

࢞ࢗ࢕ࣆ࣭ࣜࢵ࡞࠽ࡄࡾ⣵⬂ባ௥ㅨ࡞࠾࠾ࢂࡾ㓕⣪㜴ᐐࢰࣤࣂࢠ㐿ఎᏄࡡⳞ࢙ࣛࢨࢰ࣭ᚺ

➽

ېᶣᮇ ࿰៿ࠉථ⯢ ᾀᖲ(┬❟ᗀᓞኬ࣬⏍࿤⎌ሾ)

᳔∸⣵⬂ባࡡ◫㌶໩ࡢ⏍㛏ฦ໩࡞ఔ࠹㔔さ࡝⏍⌦⌟㇗࡚࠵ࡾ㸣࣋ࢠࢲࣤࡢୌḗ⣵⬂ባཀྵࡦ୯ ᒒ࡞Ꮛᅹࡊ㸡ࡐࡡ௥ㅨࡢ⣵⬂㛣ࡡ᥃╌࡞㛭ࢂࡾ⌟㇗࡞῕ࡂ㛭ࢂࡖ࡙࠷ࡾ㸣࣋ࢠࢲ࣒ࣤࢲ࢙ࣜࢪ ࢷ࣭ࣚࢭ㸝PME㸞ࡢ㸡ࡆࡡ௥ㅨࡡ㘵㓕⣪࡛ࡊ഼࡙ࡂ࠿㸡ࡆࡿࢅᙁࡂ㜴ᐐࡌࡾࢰࣤࣂࢠᅄᏄ㸝PME inhibitor;PMEI㸞࠿࢞ࢗ࢕ࣆ࣭ࣜࢵᯕᐁࡻࡽ㎾ᖳ༟㞫ࡈࡿࡒ㸣ᠻࠍࡢ㸡ࡆࡡࢰࣤࣂࢠࡡ⣵⬂ባ௥

ㅨ࡞㛭ࢂࡾᶭ⬗ࢅ᪺ࡼ࠾࡞ࡌࡾࡒࡴ㸡PMEI㐿ఎᏄࢅ༟㞫ࡊ㸡ࡐࡡິឺࢅฦᏄ⏍⌦ᏕⓏ࡞よᯊ ࡌࡾࡆ࡛ࢅモࡲࡒ㸣ࡆࡿࡱ࡚PMEI㐿ఎᏄࡡࣈ࣓࣭ࣞࢰ࣭㡷ᇡࢅ༟㞫ࡊ㸡ࡆࡡ㒼า୯࡞Ⳟ࢙ࣛ

ࢨࢰ࣭࡞ᚺ➽ࡌࡾ㒼าࢅぜ࠷ࡓࡊ࡙࠷ࡾ㸣ࡐࡆ࡚ᮇ◂✪࡚ࡢ㸡࢞ࢗ࢕ࣆ࣭ࣜࢵ⑋ཋⳞ㸧⛸࠾ࡼ

ㄢᩒࡊࡒ࢙ࣛࢨࢰ࣭ᾦ࡞ᑊࡌࡾ PMEI,ཀྵࡦࡆࡿࡱ࡚࢙ࣛࢨࢰ࣭ᚺ➽ᛮࡡሒ࿈ࡡ࠵ࡾ࣎ࣛ࢝ࣚ

ࢠࢵࣞࢻ࣭ࢭ࢕ࣤࣃࣄࢰ࣭㐿ఎᏄ(PGI)ࢅ⏕࠷࡙ࣛ࢓ࣜࢰ࢕࣑PCRἪ࡞ࡻࡽࡆࡿࡼ㐿ఎᏄࡡⓆ

⌟ᚺ➽ࢅㄢ࡬ࡒ㸣ࡐࡡ⤎ᯕ㸡PMEI㐿ఎᏄࡡⓆ⌟ࡢ㸡ᮇᐁ㥺࡚⏕࠷ࡒ㸧⛸ࡡ࢙ࣛࢨࢰ࣭࡞ᑊࡌ

ࡾᚺ➽࡞࠽࠷࡙㸡୦⩽࡛ࡵ᪺ࡼ࠾࡝ㄇᑙࡢふᐳ࡚ࡀࡍ㸡ᑊ↯༇࡛ࡡᕣ␏ࡢヾࡴࡼࡿ࡝࠾ࡖࡒ㸣

ࡱࡒ㸡ࡆࡿࡱ࡚࡞⑋ཋⳞ࢙ࣛࢨࢰ࣭࡞ᚺ➽ࡌࡾ࡛ሒ࿈ࡈࡿ࡙࠷ࡾPGI࡚ࡢ㸡᪺ࡼ࠾࡝Ⓠ⌟ቌຊ

࠿ぜࡼࡿ㸡PGI 㐿ఎᏄࡡ⑋ཋⳞ࢙ࣛࢨࢰ࣭࡞ᑊࡌࡾㄇᑙᛮ࠿࢞ࢗ࢕ⴝ࡞࠽࠷࡙ࡵ☔ヾࡈࡿࡒࠊ ࡆࡿࡻࡽᮇᐁ㥺⣌࡞࠽࠷࡙ࡢ㸡PMEIࡢ⑋ཋⳞ࡞ᑊࡌࡾ᪺☔࡝ᚺ➽ᛮࡢⓆᥱࡊ࡙࠷࡝࠷࡛♟ြ

ࡈࡿࡒ㸣

B 2

ẴᏇࡡⓆ㐡࡞㛭ࢂࡾࢨࣞ࢕ࢽࢻࢫࢻ㐿ఎᏄࡡⓆ⌟โᚒ㡷ᇡࡡよᯊ

ې㉝ᆊ ⿩ஒࠉᕖྡྷ ㄌࠉ୯ᕖ ᙁ㸝ᓞ᰷ኬ࣬⥪⛁ࢬࣤࢰ࣭ࠉ^㸧⏍∸㈠″㸞

ẴᏇࡢ᳔∸ࡡ࢝ࢪஹᥦ࡞࠽ࡄࡾ㔔さ࡝഼ࡀࢅࡊ࡙࠷ࡾࠊẴᏇࡢ㸧಴ࡡᏇ㎰⣵⬂࡚ᙟᠺࡈࡿࡾ

࠿ࠉࢨࣞ࢕ࢽࢻࢫࢻ࡞࠽࠷࡙ẴᏇࡡฦᕱࡷ㒼⨠ࠉᏇ㎰⣵⬂ࡡᙟឺ࡞㛭ࡌࡾን␏మ࠿༟㞫ࡈࡿࠉ よᯊ࠿㐅ࡴࡼࡿ࡙࠷ࡾࠊࡐࡿࡼࡡ࠹ࡔTOO MANY MOUTHS (TMM)ࠉSTOMATAL DENSITY AND

DISTRIBUTION1 (SDD1)ࠉMC79㐿ఎᏄࡢ࠷ࡍࡿࡵᏇ㎰⣵⬂⣌ㆍ(࣒ࣛࢪࢷ࣓࢕ࢺ-Ꮗ㎰ẍ⣵⬂

-Ꮗ㎰⣵⬂)࡚Ⓠ⌟ࡌࡾࡵࡡࡡⓆ⌟ࢰ࢕࣐ࣤࢡࡢ␏࡝ࡖ࡙࠽ࡽࠉࡆࡿࡼࡡⓆ⌟โᚒᶭᵋ࡞⮾࿝࠿

ࡵࡒࡿࡾࠊࡐࡆ࡚ᮇ◂✪࡚ࡢࠉᏇ㎰⣵⬂ࡡⓆ㐡ࢪࢷ࣭ࢩ࡞࠽࠷࡙ࡆࡿࡼࡡⓆ⌟ㄢ⟿࠿࡜ࡡࡻ࠹

࡞࡝ࡈࡿ࡙࠷ࡾࡡ࠾ㄢ࡬ࡾࡒࡴ࡞ࠉࢨࢪ㡷ᇡࡡྜྷᏽࢅモࡲࡒࠊ୕オࡡ㐿ఎᏄ࡞ࡗ࠷࡙ࣈ࣓࣭ࣞ

ࢰ࣭㡷ᇡࡡࢸ࣭ࣛࢨࣘࣤࢅ⾔࠷ࠉࣈ࣓࣭ࣞࢰ࣭::GFP,GUSࡡᡥἪࢅ⏕࠷࡙よᯊࢅ⾔ࡖࡒࠊࡐࡡ

⤎ᯕࠉTMM࡚ࡢ㛜ጙࢤࢺࣤ୕Ὦ200bpࡡࢤࣤࢪࢹࣚࢠࢹ࡞฽ࡾࡱ࡚࣒ࣛࢪࢷ࣓࢕ࢺ࡞࠽ࡄࡾ

Ⓠ⌟࠿ふᐳࡈࡿࠉSDD1࡚ࡢ㛜ጙࢤࢺࣤ୕Ὦ250bp࡞฽ࡾࡱ࡚Ꮗ㎰ẍ⣵⬂࡞࠽ࡄࡾⓆ⌟࠿ふᐳ ࡈࡿࠉMC79࡚ࡢ㛜ጙࢤࢺࣤ୕Ὦ0.5kbp࡞฽ࡾࡱ࡚྘⤄⧂࡞࠽ࡄࡾⓆ⌟࠿ふᐳࡈࡿࡒࠊࡆࡡ㡷 ᇡහ࡞ࢨࢪ㒼า࠿Ꮛᅹࡌࡾ࡛⩻࠻ࡼࡿࡒࠊ

B 3

^{࢞ࣔࣅ࣭ࣚࣛᆵᏽ㔖5(7}⿞⨠࡞ࡻࡾ㐿ఎᏄ⤄ᥦ࠻ࢹ࣓ࢗࣞࢤࢨࡡᏽ㔖᮪௲ࡡ᳠ゞ

ې㇇⏛ ᏭᇱỜ^㸦ࠉ✕ᒜ ᾀ^㸧ࠉᮙᮟ කỄ^㸦ࠉᆊ⏛ ࡆࡍ࠻^㸧ࠉཿ஬ ⪵^㸨ࠉᶪ⏛ ࿰⨶^㸨ࠉỜ ᆊ ᏺ᫋^㸩ࠉ⡷ㆺ Ằ㞕^㸧 㸝^㸦ᗀᓞ┬❟⥪ᢇ◂ಕ⎌ࢬࠉ^㸧ᅗ❟⾠◂ࠉ^㸨㣏⥪◂ࠉ^㸩ᗀᓞኬ㝌⏍∸ᅥ

⛁Ꮥ㸞

࠘┘Ⓩ࠙࢞ࣔࣅ࣭ࣚࣛᆵᏽ㔖5(7⿞⨠(LightCycler^®system; Roche௧ୖLCS)ࡢࠉࣇࣞࢴࢠᆵᏽ 㔖5(7⿞⨠࡛ࡢ␏࡝ࡽࠉ✭Ẵࢅ፳మ࡛ࡊ࡙Ὼᗐን໩ࢅ⾔࠷ࠉᑍ⏕ࡡ࢞ࣔࣅ࣭ࣚࣛࢲ࣭ࣖࣇࢅ౐

⏕ࡌࡾ⿞⨠࡚࠵ࡾࠊࡆࡿࡱ࡚࡞LCS࡞ࡻࡾ㐿ఎᏄ⤄ᥦ࠻(GM)ࢹ࣓ࢗࣞࢤࢨࡡᏽ㔖ฦᯊ࡞࠽࠷

࡙ࠉ௙ࡡ⿞⨠࡛᭯ណ࡞␏࡝ࡾฦᯊ⤎ᯕ࠿ᚋࡼࡿࡾၡ㢗Ⅴ࠿ᣞᦤࡈࡿ࡙࠷ࡒࠊᮇ◂✪࡞࠽࠷࡙

ࡢࠉ᢫ฝ)3&࡞㉰㡚ἴฌ⌦࡛โ㝀㓕⣪ฌ⌦ࡡ๑ฌ⌦ࢅఔࡖࡒLCS࡞ࡻࡾGMࢹ࣓ࢗࣞࢤࢨᏽ 㔖ἪࡡᨭⰃࢅ⾔ࡖࡒࠊ࠘᪁Ἢ࠙GM ࢹ࣓ࢗࣞࢤࢨ㸝MON810㸞ࢅモᩩ࡛ࡊࠉQIAGEN

Genomic-tip20/G࡞ࡻࡽDNA᢫ฝࢅ⾔ࡖࡒࠊ᢫ฝDNAࢅ㉰㡚ἴὑὯᵬ࡞ࡻࡽฌ⌦ࢅ⾔ࡖࡒᚃࠉ

EcoRϨ ฌ⌦ࢅ⾔ࡖࡒࠊᏽ㔖PCRཬᚺ᮪௲ࡢཉ⏍ຘ഼┤㏳▩Ἢ࡞ᚉ࠷ࠉῺᗐ᮪௲ࡢࠉ๑ንᛮ࡚

95Υ10ฦ㛣ಕᣚᚃࠉ1ࢦ࢕ࢠࣜࢅ95Υ15⛂Υ⛂ࠉ59Υ30⛂20Υ⛂࡚50ࢦ࢕ࢠ࡛ࣜࡊ

ࡒࠊ࠘⤎ᯕཀྵࡦ⩻ᐳ࠙᢫ฝ)3&࡛ᵾ‵ࣈࣚࢪ࣐ࢺDNAࡡࡐࡿࡑࡿࡡPCRຝ⋙㛣࡚᭯ណᕣ࠿ヾ

ࡴࡼࡿ࡙࠷ࡒ࠿ࠉ᢫ฝDNAࢅ㉰㡚ἴฌ⌦࡛โ㝀㓕⣪ฌ⌦࡚๑ฌ⌦ࡊࡒ⤎ᯕࠉPCRຝ⋙㛣࡚᭯

ណᕣࡢ♟ࡈࡿ࡝࠾ࡖࡒࠊᚉࡖ࡙ࠉࢹ࣓ࢗࣞࢤࢨ᢫ฝDNAࡡ㧏ḗᵋ㏸࠿ᏽ㔖ೋ࡞ᙫ㡢ࢅ୙࠻ࡾ

ྊ⬗ᛮ࠿♟ြࡈࡿࡒࠊ᢫ฝ)3&࡞๑ฌ⌦᧧షࢅຊ࠻ࠉ☔❟ࡊࡒ᮪௲࡞ࡻࡽࠉ᧻జΊྙ⢂మモᩩ

࠽ࡻࡦᵾ‵モᩩࡡΊථ⋙ῼᏽࢅ⾔ࡖࡒ࡛ࡆࢀࠉ┷ᗐ࠽ࡻࡦ⢥ᗐࡡⰃይ࡝ῼᏽೋ࠿ᚋࡼࡿࡒࠊ ῳ㑋ࡼ㣏⾠ヽ47,15-27(2006)

B 4

ࢨࣞ࢕ࢽࢻࢫࢻ࡞࠽ࡄࡾ࢓ࣇࢨࢩࣤ㓗ࠉࢩࣔࢪ࣓ࣤ㓗࣒ࢲࣜㄇᑙẴᏇ㛚ཾࢨࢡࢻࣜఎ㐡⤊

㊪̿ࣈࣞࢷ࢕ࣤ࣌ࢪࣆ࢒ࢰ࣭ࢭ2㸶ࡡᙲ๪̿

ې㰳⸠ ├Ẇࠉ୯ᮟ ᏿╡ࠉୖ▴ 㟱᫓ࠉᮟ⏛ ⰶ⾔㸝ᒱᒜኬᏕኬᏕ㝌⮤↓⛁Ꮥ◂✪⛁㸞

᳔∸ࡢஜ⇩ࢪࢹࣝࢪ࡞ࡈࡼࡈࡿࡾ࡛ࠉ࢓ࣇࢨࢩࣤ㓗㸝ABA㸞⏐⏍࠿ㄇᑙࡈࡿࡾࠊࡐࡊ࡙ࠉࡆࡡ ABA ࡢᏇ㎰⣵⬂හ࡚ࠉὩᛮ㓗⣪⛸㸝ROS㸞ࡡ⏐⏍ࠉ⣵⬂හ࢜ࣜࢨ࣑ࢗ࢕࢛⃨ࣤᗐࡡ୕᪴ࠉ࢓

ࢼ࢛ࣤࢲࣔࢾࣜࡡὩᛮ໩࡝࡜ࢅᘤࡀ㉫ࡆࡊࠉẴᏇ㛚ཾࢅㄇᑙࡌࡾࠊKwak ࡼ(2002)࡞ࡻࡖ࡙ࠉ

ࣉࢷࣞ୔㔖మSer/Thrࢰࣤࣂࢠ㈻⬲ࣛࣤ㓗໩㓕⣪㸝PP2A㸞ࡡㄢ⟿AࢦࣇࣗࢼࢴࢹࡡRCN1࠿ ABA ㄇᑙẴᏇ㛚ཾࢨࢡࢻࣜఎ㐡ࡡ࣎ࢩࢷ࢔ࣇ࣭ࣝ࢟ࣖࣝࢰ࣭࡛ࡊ࡙ࠉ⣵⬂හ࢜ࣜࢨ࣑ࢗ࢕࢛

⃨ࣤᗐࡡ୕᪴ࡻࡽࡵ୕Ὦ࡚ᶭ⬗ࡊ࡙࠷ࡾࡆ࡛࠿♟ࡈࡿࡒࠊୌ᪁ࠉABA ࡛ྜྷᵕ࡞ẴᏇ㛚ཾࢅㄇ ᑙࡌࡾࡆ࡛࠿▩ࡼࡿ࡙࠷ࡾࢩࣔࢪ࣓ࣤ㓗࣒ࢲࣜ㸝MJ㸞ࡢࠉABAㄇᑙẴᏇ㛚ཾࢨࢡࢻࣜఎ㐡⤊

㊪ࡡROS⏐⏍ࡻࡽ୕Ὦ࡚ࢠࣞࢪࢹ࣭ࢠࡊ࡙࠷ࡾࡆ࡛࠿ሒ࿈ࡈࡿࡒࠊࡐࡆ࡚ࠉᮇ◂✪࡚ࡢABAࠉ MJㄇᑙẴᏇ㛚ཾࢨࢡࢻࣜఎ㐡࡞࠽ࡄࡾRCN1ࡡᙲ๪ࢅࡈࡼ࡞よ᪺ࡌࡾࡆ࡛ࢅ┘Ⓩ࡛ࡊࠉࢨࣞ

࢕ࢽࢻࢫࢻࡡRCN1㐿ఎᏄࢿࢴࢠ࢓ࢗࢹን␏మ࡚࠵ࡾrcn1ን␏మࢅ⏕࠷࡙ᐁ㥺ࢅ⾔ࡖࡒࠊrcn1 ን␏మࡢࠉ௧๑ࡡሒ࿈㏳ࡽẴᏇ㛚ཾ࡞㛭ࡊ࡙ABA࡞㟸វུᛮࢅ♟ࡊࡒࠊࡱࡒࠉMJ࡞ࡵ㟸វུ

ᛮࢅ♟ࡌࡆ࡛࠿ฦ࠾ࡖࡒࠊࡈࡼ࡞ࠉROS⏐⏍㔖ࡡῼᏽ࡞ࡻࡽࠉrcn1ን␏మࡢABAࠉMJ ࡞ࡻ

ࡖ࡙Ꮗ㎰⣵⬂හ࡚ROSࢅ⏐⏍ࡊ࡝࠷ࡆ࡛࠿ฦ࠾ࡖࡒࠊࡆࡿࡼࡡࡆ࡛࠾ࡼࠉRCN1ࡢABAࠉMJ ㄇᑙẴᏇ㛚ཾࢨࢡࢻࣜఎ㐡⤊㊪࡞࠽࠷࡙ROS⏐⏍ࡡ୕Ὦ࡚ᶭ⬗ࡊ࡙࠷ࡾࡆ࡛࠿♟ြࡈࡿࡒࠊ

21 

B 5

Antioxidant defense mechanisms of proline and glycinebetaine in tobacco suspension cells against salt stress-induced oxidative damage

○Md. Anamul Hoque, Mst. Nasrin Akhter Banu, Eiji Okuma, Katsumi Amako*, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata

Graduate School of Natural Science and Technology, Okayama University, *Faculty of Nutrition, Kobe Gakuin University㸞

Plants accumulate proline and glycinebetaine to mitigate damaging effects of salt stress. We investigated effects of proline and glycinebetaine on the growth, lipid peroxidation and antioxidant enzyme activities in tobacco BY-2 suspension cells under salt stress. Both proline and glycinebetaine mitigated the inhibition of growth of BY-2 cells which was accompanied by an increased accumulation of proline and glycinebetaine under salt stress, and the mitigating effect of proline was more than that of glycinebetaine.

Salt stress increased lipid peroxidation and decreased superoxide dismutase, catalase and peroxidase activities. Exogenous proline or glycinebetaine reduced lipid peroxidation, and alleviated the reduction in catalase and peroxidase activities but not superoxide dismutase activity under salt stress. Furthermore, proline was found to be effective in reducing lipid peroxidation and alleviating the inhibition of catalase and peroxidase activities under salt stress. Neither proline nor glycinebetaine had any direct protective effect on NaCl-induced antioxidant enzyme activity. It is concluded that proline mitigates the detrimental effects of salt stress more than glycinebetaine because of its superior ability to increase the activities of antioxidant enzymes and to reduce lipid peroxidation.

B 6

᳔∸ࡡࣈࣞࣛࣤ⏍ྙᠺ࣬௥ㅨ⤊㊪ࡡよ᪺࡛⪇ሲᛮ⋋ᚋ࡞㛭ࡌࡾ◂✪

ې㧏ᶣ ⿩᫓ࠉ᳔ᆀ ὊᏄࠉ❟▴ ⬗ᏄࠉỜᆊ ᏺ᫋㸝ᗀኬ㝌࣬⏍∸ᅥ⛁Ꮥ◂✪⛁㸞

ࣈࣞࣛࣤ㸝5UR㸞ࡢ᳔∸࡚ࡢሲ࣬ஜ⇩࡝࡜ࡡ࢛ࢪ࣓ࢷ࢔ࢴࢠࢪࢹࣝࢪୖ࡞࠽࠷࡙⣵⬂හ࡞ኬ 㔖࡞⵫✒ࡊࠉᾈ㏩ᅸㄢ⟿ࢅ⾔࠹㐲ྙ⁈㈻࡛ࡊ࡙ᶭ⬗ࡊ࡙࠷ࡾࠊProࡢࠉࢡࣜࢰ࣐ࣤ㓗㸝Glu㸞࠾

ࡼ୯㛣మ࡚࠵ࡾΔ¹-pyrroline-5-carboxylate 㸝P5C㸞ࢅ⤊࡙ྙᠺࡈࡿࡾ࠿ࠉP5C synthetase 㸝P5CS㸞

࡛P5C reductase㸝P5CR㸞࡛࠷࠹㓕⣪࠿り፳ࡊ࡙࠷ࡾࠊୌ᪁ࠉProࡡ௥ㅨ⤊㊪ࡵ▩ࡼࡿ࡙࠽ࡽࠉ

Pro࠾ࡼGlu࡫࡛ฦよࡈࡿࡾ㝷࡞proline dehydrogenase㸝ProDH㸞࡛P5C dehydrogenase㸝P5CDH㸞

࡛࠷࠹㓕⣪࠿഼࠷࡙࠷ࡾࠊࡱࡒࠉGlu࠾ࡼࡡྙᠺ⣌ࡡ௙࡞ࠉ࢛ࣜࢼࢲࣤ㸝Orn㸞࠾ࡼࡡProྙᠺ

⤊㊪࠿Ꮛᅹࡊ࡙࠽ࡽࠉOrnithine-δ-aminotransferase㸝δ-OAT㸞࡛࠷࠹㓕⣪࠿り፳ࡊࠉP5C࡫࡛ྙ

ᠺࡈࡿPro࠿ྙᠺࡈࡿࡾࠊ

ᮇ◂✪࡚ࡢࠉࢰࣁࢤ࠽ࡻࡦ࢕ࢾᇰ㣬⣵⬂ࢅ⏕࠷࡙◂✪ࢅ⾔ࡖࡒࠊࢰࣁࢤᇰ㣬⣵⬂ࡡ ProDH 㐿ఎᏄࢅRNAiἪ࡞ࡻࡽᢒโࡌࡾ࡛Proྱ㔖࠿ቌຊࡊࡒࠊPro㧏ྱ㔖ᙟ㈻㌹ᥦࢰࣁࢤᇰ㣬⣵⬂

ࡢࠉሲ࠽ࡻࡦ࢛ࢪ࣓ࢷ࢔ࢴࢠࢪࢹࣝࢪ࡞ᑊࡊ࡙⪇ᛮࢅ♟ࡌ࡛࡛ࡵ࡞ࠉ⣵⬂ฦ⿛࠿Ὡᛮ໩ࡈࡿቌ Ṣ㏷ᗐࡵቌኬࡊࡒࠊࡆࡡ⤎ᯕ࡞ࡻࡽࠉ㐲ྙ⁈㈻࡚࠵ࡾProࡡ⵫✒࠿ሲ࠽ࡻࡦ࢛ࢪ࣓ࢷ࢔ࢴࢠࢪ ࢹࣝࢪ⪇ᛮࡡ⋋ᚋ࡞ࡗ࡝࠿ࡾࡆ࡛࠿᪺ࡼ࠾࡛࡝ࡽࠉຊ࠻࡙⣵⬂ᠺ㛏ࡡಀ㐅࡞ࡵࡗ࡝࠿ࡾྊ⬗ᛮ

࠿♟ࡈࡿࡒࠊ༟Ꮔⴝ࣓ࢸ᳔ࣜ∸࡚࠵ࡾ࢕ࢾᇰ㣬⣵⬂࡞࠽࠷࡙Pro῟ຊ᫤࡞ProDH࠿ࠉNaCl῟ ຊ᫤࡞P5CR࠿ࡐࡿࡑࡿⓆ⌟ቌຊࡊࡒࠊୌ᪁ࠉδ-OATࠉP5CDH࡞ࡗ࠷࡙ࡢProࡱࡒࡢNaCl῟ ຊ᫤࡞Ⓠ⌟ንິࡢࡊ࡝࠾ࡖࡒࠊ㻃

B 7

ࢰࣁࢤ࡞࠽ࡄࡾ࢓ࢪࢤࣜࣄࣤ㓗⏍ྙᠺ⤊㊪࡛⏍⌦ᶭ⬗ࡡよ᪺

ېᆊᮇ ┷྿ࠉ⚗Ễ ୌᠺࠉỜᆊ ᏺ᫋㸝ᗀኬ㝌࣬⏍∸ᅥ⛁Ꮥ◂✪⛁㸞

᳔∸࡞࠽ࡄࡾ࢓ࢪࢤࣜࣄࣤ㓗࡞ࡗ࠷࡙ࠉ⏍ྙᠺ⤊㊪ࡷ⏍⌦ᶭ⬗ࡡよ᪺࡞ࡳࡄ࡙ᵕࠍ࡝◂✪࠿

⾔ࢂࡿ࡙ࡀ࡙࠷ࡾ࠿ࠉ⌟ᅹ࡞࠽࠷࡙ࡵᮅよ᪺࡝㒂ฦ࠿ኣ࠷ࠊᮇ◂✪࡚⏍⌦ᶭ⬗࡞ࡗ࠷࡙ࠉ᳔∸

ࡡ୹さ࢓ࢪࢤࣜࣄࣤ㓗⏍ྙᠺ᭩⤂㓕⣪࡚࠵ࡾ L-galactono-1,4-lactone dehydrogenase(GalLDH)ࡡ

Ⓠ⌟ᢒโ࡛㐛๨Ⓠ⌟ࡡࡐࡿࡑࡿࡡᙟ㈻㌹ᥦ㸷㹎㸢㸧ࢅ⏕࠷ࠉᙟឺᏕⓏふᐳࢅ⾔ࡖࡒࠊࡐࡡ⤎ᯕࠉ 㐛๨Ⓠ⌟ᰬ࡞࠽࠷࡙ࡢ⣵⬂ྜྷኃࡡฆ㞗࠿ぜࡼࡿࠉⓆ⌟ᢒโ࡞࠽࠷࡙ࡢ⣵⬂ᙟឺࡡఘ㛏࠿ふᐳࡈ

ࡿࡒࠊࡈࡼ࡞ࠉ࢓ࢪࢤࣜࣄࣤ㓗ࢅᇰᆀ࡞῟ຊࡌࡾࡆ࡛࡚Ⓠ⌟ᢒโᰬࡡ⣵⬂ఘ㛏࠿ᢒโࡈࡿࡾല

ྡྷ࠿ࡲࡼࡿࡒࠊࡆࡡࡆ࡛࠾ࡼࠉ᳔∸࡞࠽࠷࡙࢓ࢪࢤࣜࣄࣤ㓗ࡢࠉ⣵⬂ฦ⿛ࡷ⣵⬂ఘ㛏࡛࠷ࡖࡒ

᳔∸ࡡᠺ㛏࡞ᙫ㡢ࢅཀྵ࡯ࡌࡆ࡛ྊ⬗ᛮ࠿♟ြࡈࡿࡒࠊ

ḗ࡞⏍ྙᠺ⤊㊪࡞㛭ࡊ࡙ࠉ㎾ᖳ࢞ࢗ࢕࠾ࡼ᩺ぞ࡞ࢠ࣭ࣞࢼࣤࢡࡈࡿࡒL-galactose-1-phosphate

phosphatase(GPPase)࡞ࡗ࠷࡙ࠉࢰࣁࢤࡡcDNAࢠ࣭ࣞࢼࣤࢡࢅ⾔ࡖࡒࠊࡐࡡ⤎ᯕ࢞ࢗ࢕ࡷࢨࣞ

࢕ࢽࢻࢫࢻࡡ,55FVH ࡛㧏࠷┞ྜྷᛮ࠿ࡲࡼࡿࠉࡱࡒInositol monophosphatase(IMP)࣓ࢲ࣭ࣆࢅ᭯

ࡊ࡙࠷ࡾࡆ࡛࠿ฦ࠾ࡖࡒࠊࡱࡒᠩ⃦ᇰ㣬㸷㹎㸢㸧࡚ࡢ࢓ࢪࢤࣜࣄࣤ㓗࡛GPPaseⓆ⌟࡛࡞┞㛭

࠿ぜࡼࡿࠉࡈࡼ࡞GPPaseⓆ⌟ᢒโᰬࢅよᯊࡊࡒ࡛ࡆࢀ࢓ࢪࢤࣜࣄࣤ㓗ྱ㔖ࡡ఩ୖലྡྷ࠿ࡲࡼ

ࡿࡒࠊࡊࡒ࠿ࡖ࡙GPPaseࡢ࢓ࢪࢤࣜࣄࣤ㓗ྱ㔖ࢅโᚒࡌࡾྊ⬗ᛮ࠿♟ြࡈࡿࡒࠊ

B 8

^{㉰ይ⇍࢓࣭࢞࢓}Sulfolobus tokodaii strain7⏜ᮮ1࢓ࢪࣂࣚ࢟ࣤ㓗࢛࢞ࢨࢱ࣭ࢭࡡ=⥲⤎ᬏᵋ

㏸よᯊ

ېὰ஬ ୌషࠉ⡷⏛ ୌᠺࠉḀᗖ ᫋ᙢ㸝ᚠᓞኬ㝌࣬ࢮࢨ࢛ࢷࢠࢿࢦ࢕࢙ࣤࢪ◂✪㒂㸞ࠉኬᓞ ᨼ஁㸝ஐᕗኬ㝌࣬㎨Ꮥ◂✪㝌㸞

࠘┘Ⓩ࠙NAD ࡢ⏍∸࠿⏍⫩ࡌࡾ୕࡚ᚪ㡪ࡡ⿭㓕⣪࡚࠵ࡽࠉࡐࡡ⏍ྙᠺ⤊㊪ࡢ⏍∸⏲࡞ᗀࡂᏋ ᅹࡌࡾࠊࡊ࠾ࡊ࡝࠿ࡼཋᰶ⏍∸ࡡde novo NAD⏍ྙᠺ⤊㊪ࡡ㛭㏻㓕⣪࡞ࡗ࠷࡙ࡢᵋ㏸ࡷཬᚺࡡ レ⣵࠿ᮅࡓ᪺ࡼ࠾࡞ࡈࡿ࡙࠷࡝࠷ࡵࡡ࠿ኣ࠷ࠊౚ࠻ࡣࠉࡆࡡ⤊㊪ࡡ➠ୌṹ㝭࡞న⨠ࡌࡾ 1࢓

ࢪࣂࣚ࢟ࣤ㓗࢛࢞ࢨࢱ࣭ࢭ(LAO)ࡢࠉኬ⭘Ⳟࡡࡲ࡚ࡊ࠾ᵋ㏸࠿よ࠾ࡿ࡙࠽ࡼࡍᵋ㏸᝗ሒ࠿இࡊ

࠷ࠊᠻࠍࡢࠉ㧏࠷⇍Ꮽᏽᛮࢅ♟ࡌLAO࠿㉰ይ⇍Ⳟ࡞Ꮛᅹࡌࡾࡆ࡛ࢅ᪜࡞᪺ࡼ࠾࡞ࡊ࡙࠷ࡾ࠿ࠉ ࡐࡡ⪇⇍ᛮ࡞ᐞ୙ࡌࡾ≁ᚡࡢ୘᪺࡚࠵ࡾࠊᮇ◂✪࡚ࡢࠉ㉰ይ⇍࢓࣭࢞࢓Sulfolobus tokodaii strain7

࡞ぜฝࡊࡒ⪇⇍ᛮLAOࡡX⥲⤎ᬏᵋ㏸よᯊࢅ⾔ࡖࡒࡡ࡚ሒ࿈ࡌࡾࠊ

࠘᪁Ἢ࣬⤎ᯕ࠙S. tokodaii strain7⏜ᮮLAO㐿ఎᏄࡡኬ⭘Ⳟ࡞࠽ࡄࡾⓆ⌟⣌ࢅᵋ⠇ࡊࡒࠊ⏐∸ࢅࠉ

⇍ฌ⌦ࠉButyl TOYOPEARL࣑࢜ࣚࢠ࣏ࣞࢹࢡࣚࣆ࢔࣭࡞ࡻࡽ⢥⿿ࡊࠉࢨࢴࢷ࢔ࣤࢡࢺࣞࢴࣈ

ⵠẴᣉᩋἪ࡚⤎ᬏ໩ࡈࡎࡒࠊX⥲⤎ᬏᵋ㏸よᯊࢅ⾔࠷ࠉ㔔ཋᏄྜྷᙟ⨠ᥦἪࢅ⏕࠷࡙2.09 Åࡡฦ よ⬗࡚ᵋ㏸Ửᏽ࡞ᠺຉࡊࡒࠊࡆࡿࡢ㉰ይ⇍Ⳟ⏜ᮮLAO࡛ࡊ࡙ࡢิࡴ࡙ࡡᵋ㏸よᯊౚ࡚࠵ࡾࠊ ኬ⭘Ⳟ⏜ᮮࡡLAO࡛Ẓ㍉ࡊࠉ⪇⇍ᛮ࡞ᐞ୙ࡌࡾ࡛⩻࠻ࡼࡿࡾ≁ᚡࢅよᯊࡊࡒࠊ

23 

B 9

㕪㗨ࢪࣚࢡࢅ⏕࠷ࡒකྙᠺ⣵Ⳟ࡞ࡻࡾỀ㈻Ὧ໩࡛⌓㓗ሲࡡ⁈ฝ

㹄⣵ᕖ 㞕ୌࠉᕖᮇ బኯࠉ➁㔕 ೸ḗࠉఫࠍᮄ ೸ࠉ㐪⸠ ᨼ㑳 㸝ᗀᓞᅗ㝷Ꮥ㝌ኬᏕ ࣁ࢕࢛࣬ࣛࢦ࢕ࢠࣜᏕ⛁㸞

⿿㕪ᡜ࠾ࡼᤴฝࡈࡿࡾ㕪㗨ࢪࣚࢡࡡ᭯ຝฺ⏕ࡡࡒࡴ࡞ࠉ㕪㗨ࢪࣚࢡࢅኣᏇ㈻໩ࠉ්↕ᠺࢅ⾔࠷ࠉ

ᤴỀฌ⌦࡞⏕࠷ࡼࡿࡾකྙᠺ⣵ⳞࠉRhodobacter sphaeroides Sࡡฆ㞗ᛮን␏ᰬ㸝㹈㸾㹈㸞ᰬࢅ⏕

࠷࡙ࠉ࢓ࣜ࢟ࣤ㓗ࢅ⿭ຐⓏ࡞౐⏕ࡊ࡙ᅖᏽ໩ࡊࠉࡱࡍỀ㈻Ὧ໩ࢅモࡲࡒࠊࡱࡒࠉᾇᇡࡡ⸬ሔ⫩

ᠺࡡࡒࡴ࡞ࠉᰜ㣬ሲࡡ⁈ฝࡡ᳠ゞࢅ⾔࡝ࡖࡒࠊ⣑1x3cmࡡኣᏇ㈻㕪㗨ࢪࣚࢡ ಴ࢅ 㹁ᐖ ࡡᐖჹ࡞ථࡿࠉெᕝୖỀ800mLࢅἸථࡊ࡙ࠉ㏳Ẵ㸝1vvm㸞ࡊࡗࡗࠉὯ໩ࢅ⾔࡝ࡖࡒࠊࢪࣚࢡ 25಴Ἰථࡊࡒࡵࡡࡢࠉ⣑4᪝ࡡὯ໩࡚㸸㹄㸹ࡢ⣑90%㝎ཡࡈࡿࡒࠊࣛࣤཀྵࡦ◢㓗ឺ✽⣪ࡵ⣑

70-80%㝎ཡࡈࡿࡒࠊୌ᪁ࠉ㕪㗨ࢪࣚࢡࡡࡲሔྙࡢࠉ㸸㹄㸹ࡢ⣑ 50%ࡊ࠾㝎ཡࡈࡿࡍࠉࣛࣤࡷ

✽⣪ࡵ࡮࡛ࢆ࡜㝎ཡࡈࡿ࡝࠾ࡖࡒࠊ㕪㗨ࢪࣚࢡ࡛කྙᠺ⣵Ⳟࡡ⤄ࡲྙࢂࡎ࡚ࠉỀ㈻Ὧ໩࠿ྊ⬗

࡝ࡆ࡛࠿᪺ࡼ࠾࡛࡝ࡖࡒࠊࡈࡼ࡞ࠉᾇỀ࡚ࡡỀ㈻Ὧ໩ᶭ⬗࡛ᰜ㣬ሲ⁈ฝ࡞ࡻࡾ⸬ሔࡡ⫩ᠺ࡝࡜

ࢅᛍ㢄࡞ࠉ㕪㗨ࢪࣚࢡ5-10 ಴ࢅ⏕࠷࡙ࠉྜྷࡋࡻ࠹࡞කྙᠺ⣵Ⳟࢅᅖᏽ໩ࡊࡒࡵࡡࢅ⏕࠷࡙ࠉ

ெᕝᾇỀ࡚ࡡᰜ㣬ሲࡡ⁈ฝࢅ᳠ゞࡊࡒࠊࡐࡡ⤎ᯕࠉ4᪝࡚ࠉකྙᠺ⣵Ⳟᅖᏽ໩ࡡࢪࣚࢡ࠾ࡼࡢ 㸸㹄㸹ࡷࣛࣤ㓗ሲࡡ⁈ฝ࠿ヾࡴࡼࡿࡒࠊ≁࡞⌓㓗ሲࡢ㕪㗨ࢪࣚࢡኣᏇ㈻ࡐࡡࡵࡡ࠾ࡼࡵ⁈ฝࡊࠉ කྙᠺ⣵Ⳟᅖᏽ໩࡚⁈ฝࡢࡈࡼ࡞ቌኬࡌࡾࡆ࡛࠿᪺ࡼ࠾࡛࡝ࡽࠉ㕪㗨ࢪࣚࢡ࡛කྙᠺ⣵Ⳟࡡᅖ ᏽ໩ᢰమ࠿ࠉ㧏⃨ᗐ࡞ỗࡿࡒỀࡡỀ㈻Ὧ໩ࡣ࠾ࡽ࡚࡝ࡂࠉẒ㍉Ⓩࡀࡿ࠷࡝ᾇᇡ࡚ࡡᰜ㣬ሲࡡ౩

⤝࡞ࡵຝᯕ࠿࠵ࡾྊ⬗ᛮ࠿ヾࡴࡼࡿࡒࠊ

B 10

Ⓠක᚜⏍∸ᅖᏽ໩ࢲࢴࣈࢅ⏕࠷ࡒ࢛ࣤࢦ࢕ࢹBODゝῼ

ې‹ཾ Ᏻ᪺ࠉᒜᓧ ┷༡ࠉ㜨ཾ ฺᩝ㸝┬❟ᗀᓞኬ࣬⎌ሾ⛁Ꮥ㸞

࠘┘Ⓩ࠙௑᪝ࠉ⎌ሾὯ໩࠿♣ఌⓏさ᭻ࡡ㧏࠷ᢇ⾙࡛ࡊ࡙⏐ᴏ⏲࠾ࡼヾ▩ࡈࡿ࡙࠷ࡾࠊ⎌ሾὯ໩

ࡡຝ⋙Ⓩ࡝ᐁ⾔ࠉᢍᥩ࡞ࡢỗ᯹⌟ሔࡡ㎷㏷࡝ࢬࣤࢨࣤࢡࡷ࣓ࢼࢰࣛࣤࢡᢇ⾙࠿୘ྊḖ࡚࠵ࡾࠊ ⁿ⩽ࡼࡢࠉࡆࡿࡱ࡚᚜⣵ຊᕝᢇ⾙࡛Ⓠක⣵Ⳟࢅ⏕࠷ࡒ᚜⏍∸࢓ࣝ࢕ࢲࢴࣈࡡష⿿ࢅ⾔࠷ࠉ'4) ゝῼ࡞㐲ࡊࡒࢲࢴࣈࢨࢪࢷ࣑ࡡ㛜Ⓠࢅ࠽ࡆ࡝ࡖ࡙ࡀࡒࠊᮇ◂✪࡚ࡢࠉ᭞࡞ྜྷࢲࢴࣈࢨࢪࢷ࣑ࢅ

⏕࠷ࡒ࢛ࣤࢦ࢕ࢹ࡞࠽ࡄࡾ᭯ᶭỗ⃦㸝BOD㸞ࡡ㎷㏷࡝᳠ฝ࡞ࡗ࠷࡙᳠ゞࡊࠉᐁ⎌ሾ࡞࠽ࡄࡾ㎷

㏷࡝'4) ゝῼࡡᐁ⾔ࠉපᏽἪ࡞ࡻࡾೋ࡛ࡡẒ㍉ࢅ⾔ࡖࡒࠊ

࠘᪁Ἢࠉཀྵࡦ⤎ᯕ࠙࢓ࢠࣛࣜᯀ㸝3cm x 7cm㸞࡞├ᙼ1000µm㸝῕ࡈ100µm㸞ࡡ࣭࣌ࣜࢅຊᕝ ࡊࠉⓆක⣵Ⳟࢅ࢓ࣜ࢟ࣤ㓗ࢣ࡚ࣜᅖᏽ໩ࡊࡒࠊࢲࢴࣈ࠾ࡼࡡ⏍∸Ⓠකࡢᕰ㈅ࡡࢸࢩࢰ࣒ࣜ࢜ࣚ

࡝࡜ࢅฺ⏕ࡊࡒ࢛ࣤࢦ࢕ࢹᑊᚺᆵ⠾᪾ῼᏽࢨࢪࢷ࣑ࢅ㛜Ⓠࡊࠉࢲࢴࣈ࠾ࡼࡡⓆකࢅ᧔ᙫࡊࡒࠊ

ࡱࡒࠉScionImage࡞ࡻࡾࢡࣝ࢕ࢪࢢ࣭ࣜよᯊ࡚Ⓠකᙁᗐࢅᩐೋ໩ࡊࠉBOD ೋࢅỬᏽࡊࡒࠊࡆ

ࡡࢨࢪࢷ࣑ࢅᐁ㝷࡞ᵕࠍ࡝࢛ࣤࢦ࢕ࢹ⎌ሾ࡞ᣚࡔ㎲ࡲࠉBOD ゝῼࢅ⾔ࡖࡒ࡛ࡆࢀࠉἑᕖࡷỗ Ềฌ⌦᪃ス࡝࡜ᐁ⎌ሾ㸝࢛ࣤࢦ࢕ࢹ㸞࡚ࡡ㎷㏷࡝BODῼᏽ࠿ྊ⬗࡚࠵ࡾࡆ࡛࠿☔ヾ࡚ࡀࡒࠊ

ࡱࡒࠉපᏽἪ࡚࠵ࡾBOD5Ἢ࡞ࡻࡾೋ࡛ࡡẒ㍉࡚ࡢ5㸚࠾ࡼ20㸚හࡡㄏᕣ࡞⏻ࡴࡼࡿࠉපᏽἪ

࡛ྜྷᵕ࡝⤎ᯕ࠿ᚋࡼࡿࡒࠊ

B 11

࢜࢞Ṿ࢓ࢠ࢓࣭࣍ࣜ࡞ࡻࡾఴ๨Ὡᛮỗἶࡡ΅ᐖ 㐪⸠ ╤ᕦ{ࠉ➁㔕 ೸ḗ{ࠉఫࠍᮄ ೸{ࠉᴃ ᨼ᪺t

㸝^㸦ᗀᓞᅗ㝷Ꮥ㝌ኬᏕ ࣁ࢕࢛࣬ࣛࢦ࢕ࢠࣜᏕ⛁㸰^㸧㸝᭯㸞࢓ࢠ࢓ࢷࢠࢿࢪ㸞

ὩᛮỗἶἪฌ⌦࡞࠽࠷࡙ࠉኣ㔖࡞ᤴฝࡈࡿࡾఴ๨ỗἶࡡฌ⌦࠿ၡ㢗࡛࡝ࡖ࡙࠷ࡾࠊ఩ࢤࢪࢹ࡚

⠾౼࡝ࢨࢪࢷ࣑࡚ఴ๨ỗἶࢅ΅ࡼࡌᢇ⾙ࡡ㛜Ⓠ࠿᭻ࡱࡿ࡙࠷ࡾࠊୌ᪁ࠉ࢜࢞ࡡṾࡢỀ㈻Ὧ໩ຝ ᯕ࠿࠵ࡾ࡛࠷࠹ࡆ࡛࠿⤊㥺Ⓩ࡞▩ࡼࡿ࡙࠷ࡾࠊᮇ◂✪࡚ࡢࠉὩᛮỗἶฌ⌦ࡡ㝷࡞࢜࢞Ṿࢅ◃ࡀ

↕ࡀᅖࡴࡒ࣭࣍ࣜ㸝࢓ࢠ࢓࣭࣍ࣜ㸞ࢅ῟ຊࡊࡒሔྙࠉ᚜⏍∸࠿࢜࢞Ṿ࣭࣍ࣜ࡞ྺ╌ࡊࠉ࠵ࡾ࠷

ࡢరࡼ࠾ࡡᠺฦࡡష⏕࡞ࡻࡽࠉὩᛮ໩ࡌࡾࡆ࡛࡞ࡻࡖ࡙ỗἶฦよࢅಀ㐅ࡊࠉὩᛮỗἶ࠿΅ᐖ໩

ࡌࡾྊ⬗ᛮࢅ᳠ゞࡊࡒࠊ㸩㹁ࡡὩᛮỗἶฌ⌦ᵬࢅモషࡊ࡙ࠉெᕝୖỀࢅ⏕࠷࡙ࠉᑊ↯ࠉ࢜࢞Ṿ

࢓ࢠ࢓࣭࣍ࣜࢅ㸩಴ࠉ㸭಴῟ຊࡊࡒ᮪௲࡚ࠉ㸧㐄㛣Ὡᛮỗἶࢅ㤾㣬໩ࡊࡒᚃࠉỗἶ΅ᐖ㸝MLSS㸞

࡛ይẴᛮ᚜⏍∸ࡡẒ⋙ࢅ᳠ゞࡊࡒࠊMLSSࡢᑊ↯࡞Ẓ࡬࡙࢜࢞Ṿ࣭࣍ࣜࢅථࡿࡒ࡮࠹࠿ࠉථࡿ

ࡾ಴ᩐ࡞ᑊᚺࡊ࡙ࠉ⣑2㸢3๪΅ᑛࡊࡒࠊฌ⌦ຝ⋙ࡢ࢜࢞Ṿ࣭࣍ࣜࡡ࠵ࡾ࡝ࡊ࡞ࡢᙫ㡢ࡈࡿ࡝

࠾ࡖࡒࠊὩᛮỗἶࡡ࿣ྺ㏷ᗐࢅẒ㍉ࡊࡒ࡛ࡆࢀࠉᑊ↯࡞Ẓ࡬࡙࠾ࡀṾ࣭࣍ࣜࢅථࡿࡒỗἶ࠿㧏

࠷࿣ྺ㏷ᗐࢅ♟ࡊࡒࠊࡱࡒࠉὩᛮỗἶ୯ࡡୌ⯙⣵Ⳟ࡞ࡗ࠷࡙ࠉይẴ᮪௲ࡷ᎒Ẵ᮪௲࡚ᇰ㣬ࡊࡒ

࡛ࡆࢀࠉ࢜࢞Ṿ࣭࣍ࣜࢅථࡿࡒ࡮࠹࠿ࠉይẴᛮ࡚ቌṢࡌࡾ⣵Ⳟ࠿ኣࡂ᳠ฝࡈࡿࡒࠊ࢜࢞Ṿ࣭࣍

ࣜࢅථࡿࡾࡆ࡛࡚ࠉ࡝ࢆࡼ࠾ࡡᠺฦ࠿ይẴᛮ⣵Ⳟࡡඁ඙ⓏቌṢࢅ࠹࡝࠿ࡊࠉይẴᛮฦよ࡞ࡻࡽ

஦㓗໩⅛⣪࡞ኣࡂ᭯ᶭ∸ࢅንᥦࡌࡾࡆ࡛࡚ࠉఴ๨ỗἶ΅ᐖຝᯕ࠿ྡྷ୕ࡌࡾലྡྷ࠿ふᐳࡈࡿࡒࠊ