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초 록
림프계의 구성 요소인 림프관은 암세포와 면역세포의 주요 이동 통로로, 염증이나 전이와 같은 여러 질병들과 밀접하게 연결되어 있다. 하지만 지금까지 복잡한 체내 림프관 환경을 모사하는 적절한 체외 림프관 모델의 부재로 인하여 암의 전이 상황에서 림프관 기능의 기제를 밝히는데 어려움이 많았다. 따라서 이 연구에서는 미세 유세 소자를 이용해 관류가능한 관으로 이루어진 3차원 림프 전이 모델을 제시한다.
사람 유래의 림프내피세포와 섬유아세포, 그리고 림프 세포의 성장을 촉진시키는 여러 성장 인자들을 이용하면 미세 유체 소자 내에서 림프관이 만들어졌다. 또한 림프관 형성은 수압차에 의해 유도되는 적절한 속력의 유동에 의해 더욱 촉진되었다. 림프관은 림프내피세포와 섬유아세포가 각각 다른 채널에 패터닝될 때만 관류되었으며, 관류된 림프관은 기저막 구성 등 체내 림프관의 기본적인 특징들을 갖고 있음이 확인되었다. 림프관은 관류된 이후 성장 인자가 없는 배지에서도 5일 이상 기존의 상태를 유지했으며, 미세 유체 소자에 주입된 암세포는 관류된 림프관 내로 들어가 내벽에 안정적으로 달라붙었다. 이 연구는