Single‐molecule DNA curtain assay

Chapter 3 High‐throughput single‐molecule imaging system using nanofabricated trenches and

3.5 Experimental methods

3.5.3 Single‐molecule DNA curtain assay

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For protein mapping, EcoRI^E111Q was labeled with quantum dots (Qdots) to visualize its binding to λ‐DNA in EcoRI buffer (40 mM Tris‐HCl [8.0], 100 mM NaCl, and 2 mM MgCl₂, 1.6% glucose, 0.1X gloxy, and 1 mM DTT).

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Na Young Cheon, Graduate student

Last updated 2022.12.07

Department of Biological Sciences

Ulsan National Institute of Science and Technology Room 605, Building 110, UNIST-gil 50

Eonyang-eup, Ulju-gun, Ulsan, South Korea

Lab Phone: (82) 52-217-2587 Mobile phone: (82) 10-9360-3579 E-mail: nycheon@unist.ac.kr

EDUCATION & RESEARCH EXPERIENCE

Combined M.S./Ph.D. course 2019 ~ 2023

B.S. in School of Life Science 2019

UNIST(Ulsan National University of Science and Technology), South Korea Advisor: Ja Yil Lee, Ph.D.

PUBLICATIONS

1. Na Young Cheon, (2022), Biophysical and biochemical approaches for R-loop sensing mechanism. Gene Expression.

2. Na Young Cheon, (2022), Studying R-loop recognizing proteins using single-molecule DNA curtain technique and electrophoretic mobility shift assay, Methods in Molecular Biology.

3. Hyun Je Kang*, Na Young Cheon*, (2020) TonEBP recognizes R-loops and initiates m6A RNA methylation for R-loop resolution, Nucleic Acids Research, 49(1):269- 284, DOI: 10.1093/nar/gkaa1162

4. Yujin Kang*, Na Young Cheon*, Jongjin Cha*, (2020) High-throughput single-molecule imaging system using nanofabricated trenches and fluorescent DNA-binding proteins.

Biotechnology and Bioengineering. 117(6):1640-1648. DOI: 10.1002/bit.27331.

5. Na Young Cheon, (2019) Single-molecule visualization reveals the damage search mechanism for the human NER protein XPC-RAD23B, Nucleic Acids Research. 47(16):8337-8347, DOI: 10.1093/nar/gkz629

*: these authors contributed equally to each work

143 AWARDS & PRESENTATIONS

1. Asan Foundation scholarship 2020~Present - Biomedical graduate scholarship

2. 2021 Young Bio Research Award 2021 - Red Ribbon Award

3. 2020 KPS(Korean Physics Society) Fall Meeting 2020 - Excellent oral presentation award

4. 2020 Institute for Basic Science, Center for Genomic Integrity Award 2020 5. 2020 Annual meeting of the Biophysical Society(BPS) 2020 - Platform presentation

6. 2019 KPS(Korean Physics Society) Spring Meeting 2019 - Oral presentation

7. 2018 International conference of KSMCB 2018 (Korean Society for Molecular and Cellular Biology)

- Excellent poster award

8. 2018 KPS(Korean Physics Society) Fall Meeting 2018 - Excellent poster award

9. UNIST excellent scholarship (Overseas scholarship) 2016

REFERENCES

Professor Ja Yil Lee School of Life Sciences

Ulsan National Institute of Science and Technology Room 601-6, Building 110, UNIST-gil 50

Eonyang-eup, Ulju-gun, Ulsan, South Korea Tel: (82) 52-217-3069

E-mail: biojayil@unist.ac.kr

144

Acknowledgement

5년의 연구실 생활 동안 물심양면으로 이끌어 주신 이자일 교수님, 정말 감사드립니다. 교수님으로부터 과학자로서 연구에 임하는 태도와 마음가짐에 대해 배웠습니다. 605호의 첫 박사 학생으로서 항상 연구 실의 미래와 후배들에 대한 책임을 마음에 새기고 더욱 정진하도록 하겠습니다.

또한 공동연구 지도에 힘써 주신 명경재 교수님, Orlando Schärer 교수님, 박사과정 동안 해 주신 진심 어 린 지도와 조언은 저의 진로와 연구에 큰 도움이 되었습니다.

이 외에도 제 논문을 심사에 참여해 주신 이창욱 교수님, 이광록 교수님께 감사드립니다. 졸업논문에 대 한 값진 조언으로 저의 과학적 시야를 넓혀 주셔서 앞으로의 연구에 큰 도움이 될 것입니다.

연구실 생활의 원동력이 되었던 유진 언니, 수빈이들, 소영이와 영서, 그리고 각자의 자리에서 최선을 다 하고 있는 친구들에게도 감사함을 전합니다. 좋은 사람들과 함께 라면 어떤 힘든 일도 즐겁게 헤쳐 나 갈 수 있음을 여러분 덕분에 배웠습니다.

특히 유진 언니는 제가 보고 싶더라도 울지 말고 언제든 저를 찾아오세요!

마지막으로 항상 저를 지지해 주고 응원해 주는 가족들에게도 감사드립니다. 여러분의 도움에 보답할 수 있는 연구자가 되도록 최선을 다하겠습니다.

앞으로도 제가 어디에 있든 우리 BioCheMP의 출신임을 잊지 않고, 연구실의 발전을 진심으로 바라겠습 니다.